scholarly journals Contamination of cattle feed with molds and mycotoxins

2013 ◽  
Vol 67 (1-2) ◽  
pp. 129-138 ◽  
Author(s):  
Vesna Krnjaja ◽  
Zeljko Novakovic ◽  
Ljiljana Stojanovic ◽  
Dusica Ostojic-Andric ◽  
Nikola Stanisic ◽  
...  

The total number of potentially toxigenic molds (fungi), total aflatoxins, zearalenone (ZON), and deoxynivalenol (DON), as well as the joint appearance of ZON and DON have been investigated in 67 samples of cattle feed (concentrate (n=21), silage of whole maize plant (n=18), beet pulp (n=4), brewer's malt (n=2), alfalfa and grass (n=1), alfalfa hay (n=12), meadow hay (n=7), pea and oat hay (n=1), and red clover hay (n=1) originating from private farms from 10 districts of the Republic of Serbia. The total number of fungi per 1 g feed ranged from 0 (silage of brewer?s malt) to 12 x 104 (concentrate). Eight fungi genus species have been identified: Acremonium, Alternaria, Aspergillus, Fusarium, Mucor, Penicillum, Rhizopus and Trichoderma. The presence of ZON (100%) was established in all the examined cattle feed samples, while 98.5% samples were contaminated with total aflatoxins and 92.5% samples were DON positive. The joint appearance of ZON and DON was established in 92.5% samples. ZON was present in the highest average concentration in the sample of alfalfa and grass silage (2477.5 ?g kg-1) and in the lowest in beet pulp silage samples (64.9 ?g kg-1). Total aflatoxins were established in the highest average concentration in the pea and oat hay silage sample (7.9 ?g kg-1) and in the lowest average concentration in beet pulp silage samples (1.6 ?g kg-1). DON was detected in the highest average concentration in concentrate samples (694.2 ?g kg-1) and in the lowest average concentration in the red clover hay sample (11.0 ?g kg-1), while DON was not detected in brewer's malt silage samples (0.0 ?g kg-1). In all the examined cattle feed samples, between moisture content (up to 20%) and the concentration of examined mycotoxins, a negative correlation was established (r=-0.26) with total aflatoxins and a positive correlation with ZON (r=0,36) and DON (r=0,60). Furthermore, a positive correlation (r=0.22) was established between ZON and DON concentrations.

2018 ◽  
Vol 8 (2) ◽  
pp. 21
Author(s):  
Hendrival Hendrival ◽  
KHAIDIR KHAIDIR ◽  
AULIA AFZAL ◽  
RAHMANIAH RAHMANIAH

The selection rice of resistant to pests S. oryzae can be done through the utilization of local paddy germplasm. The study aims to determine the level of resistance of local upland rice Aceh to against pest infestation S. oryzae. The results showed that the local paddy rice from upland Aceh has a level resistance from resistance until moderately to susceptible to pest infestations S. oryzae. The rice from varieties Depet classified in the category-resistant, while varieties Putih, Toa, and Bontok classified in the moderate category. Local rice upland Aceh from varieties Pulo Aceh and Tajuk classified in the category of moderate to susceptible to pest attack S. oryzae during storage rice. Results of correlation analysis showed that resistance rice local upland Aceh affected by moisture content (r = 0.864*; P<0.05). Results of correlation analysis showed that positive correlation non significant between resistance with characteristic dimensions of rice such as length (r = 0.106; P> 0.01), negative correlation non significant with wide (r = -0.339; P>0.01), and a positive correlation non significant with ratio (r = 0.167; P>0.01). The content of protein and moisture content of the rice had a positive correlation with the population of adult S. oryzae (moisture content: r = 0.928**; protein: r = 0.884** P<0.01), the percentage of perforated rice (moisture content: r = 0.872* P<0.05; protein: r = 0.945** P<0.01), and the percentage of rice powder (moisture content: r = 0.912**; protein: r = 0.951** P<0.01) during storage


2016 ◽  
Vol 56 (3) ◽  
pp. 541 ◽  
Author(s):  
J. Dijkstra ◽  
S. van Gastelen ◽  
E. C. Antunes-Fernandes ◽  
D. Warner ◽  
B. Hatew ◽  
...  

We quantified relationships between methane production and milk fatty acid (FA) profile in dairy cattle fed grass- or grass silage-based diets, and determined whether recent prediction equations for methane, based on a wide variety of diets, are applicable to grass- and grass silage-based diets. Data from three studies were used, encompassing four grass herbage and 14 grass silage treatments and 132 individual cow observations. Methane production was measured using respiration chambers and milk fatty acids (FAs) analysed using gas chromatography. The proportion of grass or grass silage (dry matter (DM) basis) was 0.80 ± 0.037. Methane yield averaged 22.3 ± 2.10 g/kg DM intake (DMI) and 14.2 ± 2.90 g/kg fat- and protein-corrected milk (FPCM). Mixed model univariate regression including a random study effect on intercept was applied to predict methane yield, with individual milk FA concentrations (g/100 g FA) as fixed effects. Of the 42 milk FAs identified, no single FA had a strong positive correlation (r; strong correlation defined as |r| ≥ 0.50) with methane yield (g/kg DMI), and cis-12 C18:1 and cis-9,12,15 C18:3 had a strong negative correlation with methane yield (g/kg DMI). C14:0 iso, C15:0, C15:0 iso, C15:0 anteiso, C16:0, C20:0, cis-11,14 C20:2, cis-5,8,11,14 C20:4, C22:0, cis-7,10,13,16,19 C22:5 and C24:0 had a strong positive correlation with methane yield (g/kg FPCM), and trans-15+cis-11 C18:1, cis-9 C18:1, and cis-11 C20:1 had a strong negative correlation with methane yield (g/kg FPCM). Observed methane yield was compared with methane yield predicted by the equations of van Lingen et al. (2014; Journal of Dairy Science 97, 7115–7132). These equations did not accurately predict methane yield as grams per kilogram DMI (concordance correlation coefficient (CCC) = 0.13) or as grams per kilogram FPCM (CCC = 0.22), in particular related to large differences in standard deviation between predicted and observed values. In conclusion, quantitative relationships between milk FA profile and methane yield in cattle fed grass- or grass silage-based diets differ from those determined for other types of diets.


2013 ◽  
Vol 29 (1) ◽  
pp. 145-159
Author(s):  
M. Bojanic-Rasovic ◽  
N. Nikolic ◽  
A. Martinovic ◽  
V. Katic ◽  
R. Rasovic ◽  
...  

In order to obtain good cheese quality, the milk has to possess good physical-chemical properties and should originate from healthy cows. Since milk fat and milk protein are the main constituents of cheese, their proportion in milk is of particular importance for the product yield and quality. This paper describes studies on the protein to fat ratio of milk and the consequent influence on the chemical composition and yield of semi-hard cheese, commercially called "Montenegrin naturally dried cheese". The tests were conducted on six bulk milk samples and six cheese samples. The milk parameters were analysed by the Milkoscan 400 unit whereas chemical analysis of cheese and whey were carried out with the Milkoscan FT 120 device. The average composition of the cheeses was: 29.27% fat, 21.90% protein, 55.27% total dry matter and 0.78% sodium chloride. The mean value for the content of dry matter without fat was 26%, whereas the fat content of the cheese dry matter was 53.18%, the moisture content in cheese 44.73% and moisture content in fat-free matter 63.24%. There was a medium positive correlation between the ratio of fat to protein in milk and fat content in cheese (r = 0.60309), the ratio of fat to protein in milk and dry matter of cheese (r = 0.57103), weak positive correlation between the ratio of fat to protein in milk and cheese protein (r = 0.48067) and medium negative correlation between the ratio of fat to protein in milk and moisture content in cheese (r = -0.57103). Medium negative correlation was found between the ratio of protein to fat in milk and content of cheese fat (r = -0.56416), the ratio of protein to fat in milk and cheese protein content (r = -0.51899), the ratio of protein to fat in milk and dry matter of cheese (r = -0.53118) and medium positive correlation between the ratio of protein to fat in milk and moisture content in cheese (r = -0.53118). Ratio fat to protein in milk and the actual yield of cheese was determined as medium positive (r = 0.66459) and the ratio protein to fat in milk and the actual yield of cheese as medium negative correlation (r = -0.67807). The protein to fat ratio in milk influences the decline of fat, protein, dry matter and yield of cheese and increase moisture content in cheese.


2019 ◽  
Vol 14 (2) ◽  
pp. 26-30
Author(s):  
Геннадий Осипов ◽  
Gennadiy Osipov ◽  
Зоя Осипова ◽  
Zoya Osipova

The paper includes an assessment of damageability of varieties and forms of apple by green apple aphids in the Republic of Tatarstan. An insignificant variability of damageability of leaves and annual shoots of green aphids was established by year in one half of the studied apple varieties and the average variability - in the second half of the varieties. An insignificant positive correlation is established between the degree of damage to the leaves and shoots of green apple aphids and damage to the leaves by the scab (r = 0.85), the hydrothermal coefficient of the vegetation (r = 0.78) and summer periods (r = 0.68), the amount of precipitation the summer period (r = 0.70), the average temperature in the summer period (r = 0.57) and the average temperature in April (r = 0.64). An insignificant negative correlation was found between the degree of damage to varieties and forms of apple trees by aphids and the amount of precipitation in April (r = –0.14). The manifestation of the sign “damaging the leaves and shoots of green apple aphids” is more influenced by the conditions of the year than the genotype of varieties, forms and the interaction of the genotype with the conditions of the year. The varieties Renet Povolzhya, Kamskaya and Komsin are distinguished according resistance to green apple aphid.


2014 ◽  
Vol 1 (2) ◽  
pp. 116
Author(s):  
Alimah Alimah ◽  
Yusni Ikhwan Siregar ◽  
Bintal Amin

Analysis of Ni, Mn and Cr concentrations in seawater and sediment in Singkepisland coastal waters has been conducted in March 2014. All water quality parametersmeasured were still within the tolerable limits for the survival of the aquatic organisms.Samples of sea water and sediment were taken from five stations with three replicates at eachstation. Heavy metals concentrations were analyzed in the Marine Chemistry LaboratoryFaculty of Fisheries and Marine Sciences, University of Riau by using Atomic Absorption3110 Perkin Elmer spectrophotometer. The results showed that the average concentration ofheavy metals in Singkep waters are 0.1820; 0.1084 and 0.4133 μg/L for Ni, Mn and Cr inwater, whilst in sediment are 13.2615, 13.7261 and 81.4578 μg/g for Ni, Mn and Cr. Simplelinear regression analysis indicates positive correlation between Ni in sea water and insediment with y = 10,42 + 22,19x, r = 0,138 and negative correlation with y = 15,09 -13,44xr = 0,226 for Mn and y = 104,8 - 50,76x, r = 0.354 for Cr. 


2013 ◽  
Vol 29 (3) ◽  
pp. 527-536 ◽  
Author(s):  
V. Krnjaja ◽  
J. Levic ◽  
S. Stankovic ◽  
T. Petrovic ◽  
Z. Tomic ◽  
...  

In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 ?g kg-1, 71.79 ?g kg-1, 128.17 ?g kg-1, and 1610.83 ?g kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58).


2006 ◽  
Vol 54 (4) ◽  
pp. 425-430
Author(s):  
T. Árendás ◽  
L. C. Marton ◽  
P. Bónis ◽  
Z. Berzsenyi

The effect of varying weather conditions on the moisture content of the maize grain yield was investigated in Martonvásár, Hungary from late August to late September, and from the 3rd third of September to the 1st third of Novemberbetween 1999 and 2002. In every year a close positive correlation (P=0.1%) could be observed between the moisture content in late September and the rate of drying down in October. Linear regression was used each year to determine the equilibrium moisture content, to which the moisture content of kernels returned if they contained less than this quantity of water in late September and harvesting was delayed. In the experimental years this value ranged from 15.24-19.01%.


Author(s):  
Anshelika Korolkova

The article deals with the interconnection and interdependence of phraseological semantic fields of Russian study of aphorisms in synchronic and in diachronic approaches. The correlation of phraseological semantic fields of Russian study of aphorisms is considered as their interdependence due to various factors (linguistic and extra-linguistic ones). The correlation of the phraseological semantic fields of Russian study of aphorisms is manifested in the existence of many antinomies. The natural linguistic antinomies of life / death / immortality or war / peace, or good / evil, or friend / enemy, or villainy / nobility are reflected in Russian aphorisms and have entered the corresponding phraseological semantic fields. The corpus of Russian study of aphorisms containsnot only antinomic aphorisms, but also antinomic relations that extend to the level of language and speech. Therefore, in Russian study of aphorisms there are phraseological semantic fields that implement these antinomies. In addition to the antinomic phraseological semantic fields in the corpus of classical Russian study of aphorisms there are other types of correlations. The keywords (concepts) of many phraseological semantic fields are closely thematically connected. When the number of units from one field is changed, the number of units in another phraseological semantic field also changes. Most phraseological semantic fields of Russian study of aphorisms do not show a zero correlation in either synchronic or diachronic approaches. This is due to, first of all, the universality of the aphoristic theme, with all the ideological and thematic uniqueness of the sayings used by Russian writers. However, a few phraseological and semantic fields of aphorisms by Russian writers may show a negative correlation, which is due to the diversity of the thematic groups that comprise them. A positive correlation of phraseological semantic fields, the most significant in the number of their constituent components, shows deep internal linguistic systemic connections in Russian classical study of aphorisms.


Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 1061
Author(s):  
Wajdy J. Al-Awaida ◽  
Baker Jawabrah Al Hourani ◽  
Samer Swedan ◽  
Refat Nimer ◽  
Foad Alzoughool ◽  
...  

The outbreak of coronavirus disease 2019 (COVID-19), by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has quickly developed into a worldwide pandemic. Mutations in the SARS-CoV-2 genome may affect various aspects of the disease including fatality ratio. In this study, 553,518 SARS-CoV-2 genome sequences isolated from patients from continents for the period 1 December 2020 to 15 March 2021 were comprehensively analyzed and a total of 82 mutations were identified concerning the reference sequence. In addition, associations between the mutations and the case fatality ratio (CFR), cases per million and deaths per million, were examined. The mutations having the highest frequencies among different continents were Spike_D614G and NSP12_P323L. Among the identified mutations, NSP2_T153M, NSP14_I42V and Spike_L18F mutations showed a positive correlation to CFR. While the NSP13_Y541C, NSP3_T73I and NSP3_Q180H mutations demonstrated a negative correlation to CFR. The Spike_D614G and NSP12_P323L mutations showed a positive correlation to deaths per million. The NSP3_T1198K, NS8_L84S and NSP12_A97V mutations showed a significant negative correlation to deaths per million. The NSP12_P323L and Spike_D614G mutations showed a positive correlation to the number of cases per million. In contrast, NS8_L84S and NSP12_A97V mutations showed a negative correlation to the number of cases per million. In addition, among the identified clades, none showed a significant correlation to CFR. The G, GR, GV, S clades showed a significant positive correlation to deaths per million. The GR and S clades showed a positive correlation to number of cases per million. The clades having the highest frequencies among continents were G, followed by GH and GR. These findings should be taken into consideration during epidemiological surveys of the virus and vaccine development.


Author(s):  
Abhishek Miryala ◽  
Mahendra Javali ◽  
Anish Mehta ◽  
Pradeep R. ◽  
Purushottam Acharya ◽  
...  

Abstract Background The precise timings of evoked potentials in evaluating the functional outcome of stroke have remained indistinct. Few studies in the Indian context have studied the outcome of early prognosis of stroke utilizing evoked potentials. Objective The aim of this study was to determine somatosensory evoked potentials (SSEPs) and brain stem auditory evoked potentials (BAEPs), their timing and abnormalities in acute ischemic stroke involving the middle cerebral artery (MCA) territory and to correlate SSEP and BAEP with the functional outcome (National Institutes of Health Stroke Scale (NIHSS), modified Rankin scale (mRS) and Barthel’s index) at 3 months. Methods MCA territory involved acute ischemic stroke patients (n = 30) presenting consecutively to the hospital within 3 days of symptoms onset were included. Details about clinical symptoms, neurological examination, treatment, NIHSS score, mRS scores were collected at the time of admission. All patients underwent imaging of the brain and were subjected to SSEP and BAEP on two occasions, first at 1 to 3 days and second at 4 to 7 days from the onset of stroke. At 3 months of follow-up, NIHSS, mRS, and Barthel’s index were recorded. Results P37 and N20 amplitude had a strong negative correlation (at 1–3 and 4–7 days) with NIHSS at admission, NIHSS at 3 months, mRS at admission, and mRS at 3 months and a significant positive correlation with Barthel’s index (p < 0.0001). BAEP wave V had a negative correlation (at 1–3 and 4–7 days) with NIHSS at admission, NIHSS at 3 months, mRS at admission, and mRS at 3 months and a positive correlation with Barthel’s index (p < 0.0001). Conclusion SSEP abnormalities recorded on days 4 to 7 from onset of stroke are more significant than those recorded within 1 to 3 days of onset of stroke; hence, the timing of 4 to 7 days after stroke onset can be considered as better for predicting functional outcome.


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