Stereotypical behaviour at high yielding dairy cows farms - "tongue rolling"

The objective of this work was to determine if there was a connection between stereotypical behaviour of high yielding dairy cows breeds and values of biochemical blood parameters. The investigation was carried out in august at loose-housing type of farms, in 30 heads of cattle from four groups: drying (15 to 7 days before calving), puerperium (up to 40 days after calving, early lactation (up to 120 days after calving) and late lactation (200 to 300 days after calving). Assessment of stereotypical behaviour (tongue rolling) was carried out by the method of careful observation of all the tested animals 2 to 4 hours after morning feeding. Blood samples were taken by puncture of jugular vein from 8 cows out of each animal group. In these blood samples there was determined the concentration of glucose, beta hydroxy-butyric acid (BHBA), total protein (TP), albumin, urea, total bilirubin (TBI), Ca, P, and Mg as well as AST and ALT activities. During the period up to 40 days after calving (puerperium), behavioral disorder in the form of ?tongue rolling? was found out in 4 out of 30 observed animals (13.33%). Average concentrations of all the tested blood parameters during the drying period as well as in early and late lactation were within physiological values for cattle. During puerperium there were found significantly lower values of glycaemia, proteinemia, albuminemia, uremia and magnesiemia in regard to antepartal values (p<0.05), where the values of glycaemia and magnesiemia were below the physiological limit. A the same time, in this group of cows the values of TBI and AST activities were higher than physiological values. Frequent appearance of ?tongue rolling? phenomenon only among cows in the group with deviation of biochemical parameters values, points out to a possible connection between the stereotypical behaviour and biochemical composition of blood. It seems that hypomagnesiemia could be a significant etiopathogenetic factor causing the change in behaviour of cows in intensive livestock production.

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The effects of different anticoagulants on biochemical parameters in blood of dairy cows

Veterinarski glasnik ◽

10.2298/vetgl1502013b ◽

2015 ◽

Vol 69 (1-2) ◽

pp. 13-20

Author(s):

Branislava Belic ◽

Marko Cincovic ◽

Maja Dosenovic ◽

Dragica Stojanovic ◽

Zorana Kovacevic

Keyword(s):

Alkaline Phosphatase ◽

Dairy Cows ◽

Alkaline Phosphatase Activity ◽

Total Protein ◽

Biochemical Parameters ◽

Biochemical Analysis ◽

Blood Parameters ◽

Albumin Level ◽

Blood Samples ◽

Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

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RELATIONSHIP BETWEEN KERATIN LAYER OF DUCTUS PAPILLARIS AND ZINC BLOOD SERUM CONCENTRATION IN DAIRY COWS

Archives of Veterinary Medicine ◽

10.46784/e-avm.v5i2.170 ◽

2012 ◽

Vol 5 (2) ◽

pp. 45-51

Author(s):

Ivana Davidov ◽

Miodrag Radinović ◽

Mihajlo Erdeljan ◽

Zorana Kovačević ◽

Dragica Stojanović

Keyword(s):

Dairy Cows ◽

Zinc Level ◽

Blood Samples ◽

Holstein Friesian ◽

Physiological Values ◽

Experimental Group ◽

Different Thickness ◽

Teat Canal ◽

Keratin Layer ◽

Friesian Cows

The experiment was carried out on a mini farm of Holstein-Friesian cows with 30 animals, aged between 3 and 5 years and the zinc level in serum was measured. The analyses of the blood samples indicated a variation in the concentration of zinc in serum. In 11 out of 15 tested cows (73.33%) the zinc values in serum were below the physiological values for high-milking cows. In the experimental group different thickness of the keratin layer was detected in the teat canal. The statistical analysis showed a positive correlation between the concentration of zinc in cow serum and the thickness of teat canal keratin layer. This indicates that the cows with low values of zinc have thin keratin layer of the teat canal.

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Relationship among blood metabolites and lipid content in the liver in transitional dairy cows

Biotechnology in Animal Husbandry ◽

10.2298/bah1204705d ◽

2012 ◽

Vol 28 (4) ◽

pp. 705-714 ◽

Cited By ~ 3

Author(s):

R. Djokovic ◽

H. Samanc ◽

M.D. Petrovic ◽

Z. Ilic ◽

V. Kurcubic

Keyword(s):

Dairy Cows ◽

Total Cholesterol ◽

Lipid Content ◽

Total Bilirubin ◽

Transition Period ◽

Dairy Herd ◽

Hepatic Function ◽

Transitional Period ◽

Blood Samples

The objective of the present study was to determine a relationship among blood indicators of hepatic function and lipid content in the liver during transitional period in dairy cows. Late pregnant and calved cows (n= 40) were selected from a Holstein dairy herd and allocated to four groups: a late pregnant cows (n=10) from day 15 to day 5 before calving; late pregnant cows (n=10) from day 5 to day 1 before calving; clinically puerperal healthy cows (n=10) and clinically ketotic puerperal cows (n=10). Liver and blood samples were taken from all cows. The results of present investigation have shown that the lipid content in the liver and the blood non-esterificed fatty acids, ?-hydroxybutyrate, total bilirubin concentrations and the AST activities were significantly higher (P<0.05) as well the blood glucose, total cholesterol, triglycerides and albumin concentrations were significantly lower (P<0.05) in puerperal ketotic cows as compared to the values of these parameters in the blood of healthy cows. The fat proportions in liver were positively (P<0.05) associated with the AST activities and with the serum NEFA and BHB concentrations, but negatively correlated (P<0.05) with the circulating triglyceride, total cholesterol and albumin concentrations, All these biochemical metabolites may be used as important biochemical indicators in the determination of the functional status of the liver in dairy cows during the transition period.

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A note on plasma cholecystokinin concentration in dairy cows

Animal Science ◽

10.1017/s0003356100006061 ◽

1991 ◽

Vol 53 (1) ◽

pp. 123-125 ◽

Cited By ~ 13

Author(s):

M. Furuse ◽

S. I. Yang ◽

Y. H. Choi ◽

N. Kawamura ◽

A. Takahashi ◽

...

Keyword(s):

Plasma Concentration ◽

Dairy Cows ◽

Continuous Flow ◽

Jugular Vein ◽

Experimental Period ◽

Blood Samples ◽

Lactating Cows ◽

Plasma Cholecystokinin

Changes in plasma cholecystokinin (CCK) levels after feeding were investigated in dairy cows. Three HolsteinFriesian lactating cows given concentrates at 06.00 and 13.00 h and hay at 16.30 h were used. Blood samples were taken from the jugular vein from 0 (before feeding, 13.00 h) to 6 h at 1-h intervals. The immunoreactivity of CCK was expressed in terms of the CCK-8 equivalent. The plasma concentration of CCK was not changed over the experimental period. This result might be reflected by the continuous flow of digesta from the rumen.

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Effect of Coriander Seeds (Coriandrum Sativum) on Wool Growth and It's Traits and Some Haematological Traits of Awassi Ewes

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v34i2.645 ◽

2010 ◽

Vol 34 (2) ◽

pp. 148-155

Author(s):

Drgham H. Yousif AL-Zwein

Keyword(s):

Jugular Vein ◽

Hemoglobin Concentration ◽

Blood Parameters ◽

Wool Fiber ◽

Feed Additive ◽

Control Group ◽

Blood Samples ◽

Wool Growth ◽

Physical Traits ◽

The Right

This study was conducted to find out the effect of coriander seeds as feed additive on some hematological traits and wool growth and it's physical traits of Awassi ewes, blood samples were taken biweekly for blood picture, and wool samples were taken every 2.5 months for wool growth and its physical traits. Eighteen Awassi ewes at the age of 2-3 years were randomly divided equally into three groups (6each ) "body weight was considered".Ewes in the first group kept as control and daily fed on concentrate diet (350 g/ head), while the second group was fed on the same diet contain (2.5%) of coriander seeds, while the third group was fed on same diet contain (5%) of coriander seeds. Also all animals were allowed to graze. Blood samples were taken from jugular vein for blood traits viz (PCV) packed cell volume, (Hb) hemoglobin, (MCHC) mean cell hemoglobin concentration. Wool samples (5×5) cm2 were clipped from the last six ribs of the right chest for measuring wool growth and its traits (creasy and clean wool, samples weights, clean wool ratio, staple and fiber lengths and diameters and wool fiber crimps in 1cm of length). The results of blood parameters revealed that the second and third groups were significantly (p<0.05) recorded higher values in their PCV ,Hb, MCHC during most studied periods compared with the control group ,While the second and third groups were significantly (P<0.05) recorded higher values than the first group (control) in their creasy and clean wool, fiber length, wool fiber crimps, while no differences where existed in other wool physical traits.

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Pulsatile secretion of oestradiol-17β in post-partum dairy cows

Animal Science ◽

10.1017/s0003356100032542 ◽

1989 ◽

Vol 49 (3) ◽

pp. 335-338 ◽

Cited By ~ 2

Author(s):

A. R. Peters ◽

B. M. A. O. Perera

Keyword(s):

Dairy Cows ◽

Jugular Vein ◽

Post Partum ◽

Vena Cava ◽

Ovarian Follicles ◽

Blood Samples ◽

Pulsatile Secretion ◽

Post Partum Period ◽

Plasma Oestradiol

ABSTRACTThirteen dairy cows between 7 and 20 days post partum were fitted with catheters in both the jugular vein and caudal vena cava, the latter with the tips anterior to the junction with the ovarian veins. Blood samples were taken from nine control cows from both sites at 15-min intervals for periods of between 8 and 16 h. Four more cows were sampled for 13 h and given 4 × 2·5 ug GnRH intravenously at 3-h intervals starting 1 h after sampling began. Plasma oestradiol-17(3 concentrations were significantly higher in the vena cava than in the jugular vein in the two cows in which they were compared. Twenty-eight LH pulses occurred in the nine control cows during the sampling periods. These were followed within 1 h by increases in caval oestradiol-17β concentrations in 26 cases. LH pulses occurred after 14 of the 16 GnRH injections and with one exception were associated with oestradiol pulses in the vena cava. Oestradiol concentrations generally peaked 15 to 45 min after each LH peak. It is concluded that ovarian follicles are responsive to LH pulses by releasing oestradiol pulses early in the post-partum period.

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Effect of propofol on the haematological profile of pregnant Red Sokoto does

Savannah Veterinary Journal ◽

10.36759/svj.2018.002 ◽

2019 ◽

pp. 37-40

Keyword(s):

General Anaesthesia ◽

Jugular Vein ◽

Small Animal ◽

Rapid Onset ◽

Blood Parameters ◽

Duration Of Action ◽

Blood Samples ◽

Anaesthetic Agents ◽

Haematological Profile ◽

Apparently Healthy

Introduction: Propofol (Diprivan®) is now a mainstay drug during anaesthesia in human and small animal surgeries and this is as a result of its good quality anaesthesia, rapid onset, and short duration of action with rapid recoveries. There is paucity of information in Nigeria as per the use of general anaesthesia to carry out procedures in pregnant red Sokoto Does. The aim of this study is to assess the haematological profile of pregnant red Sokoto doe following propofol anaesthesia and also ascertain the safety of the drug during gestation. Methods: Ten apparently healthy pregnant Red Sokoto does were used for this study. The animals were acclimatised for two weeks and pregnancy was diagnosed using B-mode Real time® transcutaneous ultrasonography. The animals were grouped into two (A and B) comprising five animals each in their first and second trimesters respectively. Propofol at 4 mg/kg and 5 mg/kg was administered to the does in groups A and B respectively. Blood samples were collected via the jugular vein and analysed using full automated blood counter (Ehmma® PCE 210). Results: The result showed that the onset and duration of propofol anaesthesia at the two doses in both groups were smooth and rapid without much excitation. There were no significant (p < 0.05) differences in the blood parameters in both group when compared with the control. Significance: Our study lends credence to the fact that propofol anaesthesia is suitable and safe anaesthetic agents for induction and maintenance of general anaesthesia in pregnant red Sokoto does.

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Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v12i3.7101 ◽

2017 ◽

Vol 12 (3) ◽

Author(s):

K. Rokde ◽

S. Kumar ◽

A. Bhardwaz ◽

S. S. Mahour ◽

S. P. Nema ◽

...

Keyword(s):

Eosinophil Count ◽

Jugular Vein ◽

Dairy Farm ◽

Fetal Membranes ◽

Monocyte Count ◽

Blood Samples ◽

Mean Values ◽

Crossbred Cows ◽

Haematological Profile ◽

The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

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Serum Endocrine Profile in Relation to Estrus Synchronization in Surti Does

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v13i03.10609 ◽

2018 ◽

Vol 13 (03) ◽

Author(s):

M. M. Chaudhary ◽

C. T. Khasatiya ◽

S. B. Patel ◽

S. S. Chaudhary ◽

V. B. Atara ◽

...

Keyword(s):

Jugular Vein ◽

Estrus Synchronization ◽

Progesterone Concentration ◽

Serum Progesterone ◽

Blood Samples ◽

Group Iii ◽

Group I ◽

Endocrine Profile ◽

Group Ii ◽

And Control

The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive- apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5 mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment), 11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups, 83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th (4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/ ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/ ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0 (12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and 40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level found at 40th day might be the influence of the non-pregnant does at first service in the cumulative animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy in treated and control animals.

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Characterization of Short-Term Heat Stress in Holstein Dairy Cows Using Altered Indicators of Metabolomics, Blood Parameters, Milk MicroRNA-216 and Characteristics

Animals ◽

10.3390/ani11030722 ◽

2021 ◽

Vol 11 (3) ◽

pp. 722

Author(s):

Jang-Hoon Jo ◽

Jalil Ghassemi Nejad ◽

Dong-Qiao Peng ◽

Hye-Ran Kim ◽

Sang-Ho Kim ◽

...

Keyword(s):

Heat Stress ◽

Dairy Cows ◽

Milk Yield ◽

Milk Fat ◽

Blood Parameters ◽

Negative Effects ◽

Short Term ◽

Lactating Cows ◽

Humidity Index ◽

Holstein Dairy Cows

This study aims to characterize the influence of short-term heat stress (HS; 4 day) in early lactating Holstein dairy cows, in terms of triggering blood metabolomics and parameters, milk yield and composition, and milk microRNA expression. Eight cows (milk yield = 30 ± 1.5 kg/day, parity = 1.09 ± 0.05) were homogeneously housed in environmentally controlled chambers, assigned into two groups with respect to the temperature humidity index (THI) at two distinct levels: approximately ~71 (low-temperature, low-humidity; LTLH) and ~86 (high-temperature, high-humidity; HTHH). Average feed intake (FI) dropped about 10 kg in the HTHH group, compared with the LTLH group (p = 0.001), whereas water intake was only numerically higher (p = 0.183) in the HTHH group than in the LTLH group. Physiological parameters, including rectal temperature (p = 0.001) and heart rate (p = 0.038), were significantly higher in the HTHH group than in the LTLH group. Plasma cortisol and haptoglobin were higher (p < 0.05) in the HTHH group, compared to the LTLH group. Milk yield, milk fat yield, 3.5% fat-corrected milk (FCM), and energy-corrected milk (ECM) were lower (p < 0.05) in the HTHH group than in the LTLH group. Higher relative expression of milk miRNA-216 was observed in the HTHH group (p < 0.05). Valine, isoleucine, methionine, phenylalanine, tyrosine, tryptophan, lactic acid, 3-phenylpropionic acid, 1,5-anhydro-D-sorbitol, myo-inositol, and urea were decreased (p < 0.05). These results suggest that early lactating cows are more vulnerable to short-term (4 day) high THI levels—that is, HTHH conditions—compared with LTLH, considering the enormous negative effects observed in measured blood metabolomics and parameters, milk yield and compositions, and milk miRNA-216 expression.

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