scholarly journals EFEK GEL EKSTRAK HERBA PEGAGAN (Centella asiatica L. Urban) DENGAN GELLING AGENT HIDROKSIPROPIL METHYLCELLULOSE TERHADAP PENYEMBUHAN LUKA BAKAR PADA KULIT PUNGGUNG KELINCI

Biomedika ◽  
2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Tanti Azizah Sujono ◽  
Ullya Nur Wahyu Hidayah ◽  
T.N. Saifullah Sulaiman
Keyword(s):  
Wound Healing ◽  
New Zealand ◽  
Healing Process ◽  
Hydroxypropyl Methylcellulose ◽  
Centella Asiatica ◽  
Negative Control ◽  
Gelling Agent ◽  
Wound Healing Process ◽  
Structural Protein ◽  
Burn Wound

Pegagan (Centella asiatica L. Urban) contain saponin asiaticoside, which is a structural protein. The compound plays a role in the wound healing process by the formation of collagen. This research was conducted to determine the effect of wound healing of burns on New Zealand male rabbits using gel extract of Pegagan (Centella asiatica L. Urban) herbs using hydroxypropyl methylcellulose (HPMC) as gelling agent. This research was an experimental with the same subject design, using � ve male New Zealand rabbits to test the wound healing of burns, and the backs of each rabbit were divided into six treatment i.e : positive control (Bioplasenton), negative control (no treatment), control HPMC base (no extract), and three formula with herbal extracts of Centella asiatica with a concentration of gelling agent HPMC 8%, 9%, and 10% respectively. Gel was applied to the back of rabbits with the use ± 0.3 grams once daily until the wound diameter equal to zero or until healed. The data was analyzed by one way anava with a 95% confidence level. The result showed that of the gelling agent HPMC concentration effect on the increased the viscosity, and decreased the spreadability and the burn wound healing time is a longer. Gel herb pegagan extract (Centella asiatica L. Urban) with a gelling agent HPMC concentration of 8% (17,60±1,14 days) most effectively heal burns compared to the concentration of 9% (19,40 ±1,14 days) and concentration 10% (20,40±1,14 days).Keywords: Centella asiatica L. Urban, gel, hydroxypropyl methylcellulose, burns.

scholarly journals The Effectiveness Test of Oil Phase Ointment Containing Snakehead Fish (Channa striata) Extract on Open Stage II Acute Wounded Wistar Strain Male Rats

2017 ◽  
Vol 22 (2) ◽  
pp. 97 ◽  
Author(s):  
Fransisca Daisa ◽  
Mohamad Andrie ◽  
Wintari Taurina
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Stage Ii ◽  
Male Rats ◽  
Wound Healing Process ◽  
Omega 3 ◽  
Significant Difference ◽  
Healing Power ◽  
Channa Striata

The snakehead (Channa striata) contained fatty acids omega-3 and omega-6 are useful as nutrients in accelerating the wound healing process. This study aims to determine the effectiveness of the oil phase extract ointment snakehead in the healing of acute wounds open stage II. Testing the effectiveness of wound healing using 6 groups: normal, gel bioplacenton (positive control), ointment base (negative control), ointments snakehead extract oil phase concentration of 10; 20 and 40% of the 4 rats with acute wounds open stage II and observation until day 16 injury. The area of the wound was measured with the image J program Macbiophotonic, calculated% healing power, and AUC values. A statistical test to the total AUC values per rat with SPSS for Windows 22.0 program using One Way ANOVA and Post-hoc LSD test. The results showed a significant difference in the negative group with ointment concentration of 20% (p <0.05). The treatment group phase ointment fish oil extract is effective for wound healing is best to have a concentration of 20% for percentage of wound healing power of 97.157% and the average AUC value of 865.683% of the day.

scholarly journals Bee Pollen: Chemical Composition and Therapeutic Application

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Katarzyna Komosinska-Vassev ◽  
Pawel Olczyk ◽  
Justyna Kaźmierczak ◽  
Lukasz Mencner ◽  
Krystyna Olczyk
Keyword(s):  
Wound Healing ◽  
Pathological Condition ◽  
Healing Process ◽  
Radical Scavenging ◽  
Wound Healing Process ◽  
Therapeutic Application ◽  
Burn Wound ◽  
Bee Pollen ◽  
Burn Wound Healing ◽  
Natural Medicine

Bee pollen is a valuable apitherapeutic product greatly appreciated by the natural medicine because of its potential medical and nutritional applications. It demonstrates a series of actions such as antifungal, antimicrobial, antiviral, anti-inflammatory, hepatoprotective, anticancer immunostimulating, and local analgesic. Its radical scavenging potential has also been reported. Beneficial properties of bee pollen and the validity for their therapeutic use in various pathological condition have been discussed in this study and with the currently known mechanisms, by which bee pollen modulates burn wound healing process.

scholarly journals HE EFFECT OF BINJAI LEAVES EXTRACT (Mangifera caesia) ON INCISIONAL BACK WOUND OF MICE (Mus musculus) TO THE NUMBER OF NEUTROPHIL CELLS (Preliminary Study of Drug Preparations for Wound Healing)

2020 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Desty Ayu Dwiyanti ◽  
Irham Taufiqurrahman ◽  
Didit Aspriyanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Group Design ◽  
Inflammatory Phase ◽  
Significant Difference

Background: Ethanol extract of binjai leaves contain bioactive compounds in the form of flavonoid and saponin which is potential as anti-inflammatory, so it will be able to control the wound healing process. Binjai leaves extract can affect the number of neutrophil on inflammatory phase, as it is characterized by neutrophil cells infiltration to the wound tissue, so its number will increase at the beginning of inflammatory phase and decrease on the 3rd day. Objective: To prove the effect of binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL which given topically to the number of neutrophil in day 1, 3, and 5 of wound incision on the back of male mice. Methods: The study design was a pure laboratory experimental study with post-test only with control group design which involved 36 mice divided into 4 groups: negative control group design which was given BR2 feed, treatment group which was given binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL. Results: The result of One-way ANOVA showed that there was significant difference of all groups in day 1 (p=0.000), day 3 (p=0.001), and day 5 (p=0.000). Post-Hoc LSD test showed that there was significant difference (p<0.05) between the negative control group and treatment groups. Conclusion: Binjai leaves extract has an effect to increase the number of neutrophil in day 1 and decrease the number of neutrophil in day 3 and 5 on the healing of back wound incision in mice.

scholarly journals A study on new experimental induction of wound using metal surface contact in a rat model

2020 ◽  
Vol 11 (1) ◽  
pp. 813-817
Author(s):  
Balaji K ◽  
Perumal Saraswathi ◽  
Prabhu K ◽  
Shila Samuel ◽  
Siva T
Keyword(s):  
Wound Healing ◽  
Metal Surface ◽  
Therapeutic Option ◽  
Healing Process ◽  
Research Progress ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Contact Method ◽  
Burn Wound ◽  
Surface Contact

Burns injury is a global issue; when burns left unmonitored, it may lead to sepsis & hypovolemia. It majorly contributing to the country's morbidity and mortality. Accidental burns are quite common throughout the world. The degree of burns differs variably, and to identify a possible therapeutic option, it is of great importance for the researchers and scientists. Achieving a uniform burn wound model is thus required to study the burns injury at different levels involving epidermis, dermis, and hypodermis. The goal of this study is to create a simple third-degree contact burn in Wistar albino rats. Third-degree contact burn wound was monitored on 7th, 14th, and 21st day for natural wound healing under gross and histopathological observation. Gross observation reported damaged skin to appear dry, leathery, and charred. Wound closure was observed to be delayed and incomplete on day 21. Histopathology revealed a wound healing process by hematoxylin and eosin method. This metal surface contact method revealed a simplified and cost-effective method for inducing a third degree of a wound in Wistar rats. The study is thus a milestone that could enable research progress in the area of wound healing.   

scholarly journals Efek aplikasi topikal gel ekstrak pandan wangi terhadap penyembuhan luka gingiva

2017 ◽  
Vol 2 (2) ◽  
pp. 53
Author(s):  
Icha Nofikasari ◽  
Afifah Rufaida ◽  
Chynintia Dewi Aqmarina ◽  
Failasofia Failasofia ◽  
Annisa Rahmi Fauzia ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Topical Application ◽  
Healing Process ◽  
Punch Biopsy ◽  
Positive Control ◽  
Negative Control ◽  
Wound Healing Process ◽  
Wound Area ◽  
Pandanus Amaryllifolius

The Effect of topical application pandan extract gel on gingival wound. Post-gingivectomy wound is usually covered by periodontal dressing, which generally contains some chemical compounds to protect the wound. However, it can provide allergic effect on some patients. Pandan leaves (Pandanus amaryllifolius Roxb.) contain a number of active substances that have anti-inflammatory, antioxidant and antibacterial effect and play a role in wound healing. This study aims to determine the effect of topical application of Pandan leaf extract gel on gingival wound healing. Gingivectomy model was carried out on mandibular incisive gingival using the 2.5-mm punch biopsy. Thirty-six rats were randomly divided into 3 groups of treatment: negative control (CMC-Na), positive control (Aloclair), and 50% of pandan extract gel. The gels on each group were applied (twice in a day) to the wound area after gingivectomy. The observation of the wound healing process was also carried out on day 1, 3, 7, and 14 by making the histological preparations of gingival wound area. The number of blood vessels was observed using microscope and data was analysed using Two- Way Anova and LSD. The result showed that number of blood vessel increased on day 3 and the peak was on day 7. Anova and LSD test showed several significant differences comparison the number blood vessel between treatment and control. In conclusion, topical application Pandan leaves extract gel could accelerate gingival wound healing.ABSTRAKLuka pasca gingivektomi dibalut dengan periodontal dressing yang mengandung senyawa kimia dengan tujuan melindungi luka, namun senyawa kimia periodontal dressing yang ada di pasaran dapat menimbulkan efek alergi terhadap beberapa pasien. Daun pandan wangi (Pandanus amaryllifolius Roxb.) mengandung zat aktif yang memiliki anti inflamasi, antioksidan, dan antibakteri kemungkinan berperan dalam proses penyembuhan luka. Penelitian ini bertujuan untuk mengetahui peran gel ekstrak daun pandan wangi dalam proses penyembuhan luka pasca gingivektomi pada tikus wistar melalui pengamatan jumlah pembuluh darah. Model gingivektomi dilakukan pada gingiva incisivus mandibula dengan menggunakan punch biopsy diameter 2,5 mm. Tiga puluh enam tikus dibagi secara acak ke dalam 3 kelompok perlakuan yaitu kontrol negatif (Gel CMC-Na), kontrol positif (Aloclair), dan gel ekstrak pandan wangi 50%. Gel uji pada masing- masing kelompok diaplikasikan pada area luka pasca gingivektomi dua kali sehari. Pengamatan proses penyembuhan luka dilakukan pada hari ke 1, 3, 7, dan 14 dengan membuat preparat histologi gingiva area luka. Parameter penyembuhan luka yang diamati adalah jumlah pembuluh darah. Data jumlah pembuluh darah dianalisis dengan menggunakan uji statistik parametrik. Hasil penelitian menunjukkan jumlah pembuluh darah mengalami peningkatan mulai hari ke-3 dan mencapai puncaknya pada hari ke-7. Hasil uji two way Anova menunjukkan adanya perbedaan yang bermakna rerata jumlah pembuluh darah antar kelompok perlakuan dengan kontrol positif maupun negatif (p<0,05). Hasil uji LSD juga menunjukkan perbedaan yang bermakna perbandingan kelompok kontrol dan perlakuan pada semua hari pengamatan. Kesimpulan penelitian ini adalah gel ekstrak pandan wangi 50% dapat mempercepat proses penyembuhan luka pasca gingivektomi dengan kemampuannya meningkatkan jumlah pembuluh darah.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Channa Striata ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

scholarly journals Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Ali Asghar Hemmati ◽  
Iran Rashidi ◽  
Sedigheh Dahanzadeh ◽  
Mahmoud Moeini
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Punica Granatum ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Hydroxyproline Content ◽  
Tissue Samples ◽  
Treatment Groups ◽  
Wound Recovery

Objective: This research designed to investigate the wound healing process with pomegranate hydroalcoholic seed extract (Punica granatum) in comparison with no-treatment, betamethasone, phenytoin and eucerin in rabbits. Methods: The positive group including groups that received phenytoin cream (1%) and topical eucerin, respectively, twice a day to complete wound healing. negative Control group did not obtain any treatment. Treatment groups were received cream of PSE (2, 5,7,10 w/w) in eucerin and 75% w/w as purified extract twice daily. In order to measure the percentage of wound healing, the zone of the wound was evaluated daily. Histological studies were done on the 7th and 15th days of treatment. Next, hydroxyproline content of wounds healed and tensile strength of wound tissue samples were measured. Results: The results demonstrated between PSE treatment groups and eucerin animals were statistically significant aadifferences (P<0.05) in most of the days reviewed. Treatment of Rabbits with 10% PSE had the best results (complete wound recovery in 12 days). Also, this treatment showed higher hydroxyproline content and higher tissue strength. Conclusion: This research reveals that the extract of 10% PSE administrated topically has the proper potential to induce wound recovery in the wound model of rabbits. In addition, 10% PSE accelerates the healing of the wound. Further study needs to clarify the results of this research.

scholarly journals Perbandingan Pemberian Madu Lokal Bunga Cengkeh (Syzygium aromaticum) dan Madu Impor Bunga Manuka (Leptospermum scoparium) Secara Topikal Terhadap Penyembuhan Luka Sayat Pada Mencit Putih (Mus musculus)

2019 ◽  
Vol 6 (2) ◽  
pp. 25
Author(s):  
Nikita Tenritojang Mustafa ◽  
Dini Kurnia Ikliptikawati ◽  
Abdul Wahid Jamaluddin
Keyword(s):  
Wound Healing ◽  
Povidone Iodine ◽  
Healing Process ◽  
Colour Change ◽  
Negative Control Group ◽  
Negative Control ◽  
Wound Dressings ◽  
Control Group ◽  
Wound Healing Process ◽  
Syzygium Aromaticum

ABSTRAK Luka merupakan trauma fisik mengakibatkan kulit terbuka. Luka merupakan jalur masuk bagi mikroorganisme dari luar penyebab infeksi. Dahulu madu dimanfaatkan untuk mengobati luka bakar dan luka sayat. Madu memiliki keunggulan yakni memiliki hidrogen peroksida, eugenol dan methylglyoxal yang merupakan antibiotik yang unggul. Penelitian ini dilakukan dengan uji ekperimental. Hewan coba dibagi dalam 4 kelompok. Setiap kelompok diberikan perlakukan antara lain: dengan pemberian madu lokal, madu impor, dan salep povidone iodine (kontrol positif), dan kelompok kontrol negatif tanpa perlakuan. Dilanjutkan pemantauan dan pencatatan untuk proses penyembuhan tiap kelompok. Hal ini diukur dengan indikator antara lain: luas luka, kelembaban luka, warna, serta perubahan keropeng. Pada penelitian ini ditemukan bahwa dengan pemberian madu mempercepat proses penyembuhan luka dibandingkan dengan pemberian air dan povidone iodine (p<0,05). Selain itu ditemukan madu lokal memberikan efek penyembuhan luka yang lebih cepat dibandingkan madu impor. Pemberian madu memberikan efek yang lebih baik terhadap penyembuhan luka. Dibandingkan dengan pemberian salep Povidone iodine dan tanpa perlakuan.Kata kunci : Madu Bunga Cengkeh, Madu Bunga Manuka, Waktu penyebuhan, Warna Luka, Kelembaban. ABSTRACT Wound is a physical trauma causes discontinuity, Leads physiological dysfunction. Wound is an entry point for microorganisms from outside as source of infection. In the past, honey was used to treat burns and cuts. Honey is a natural ingredient as a moist wound-dressings. Besides that, honey contain hydrogen peroxide, eugenol, and methylglyoxal which is a superior antibiotic. This research was carried out by experimental test. The experimental animals were divided into 4 groups, each group consisted of 6 animals. Each group is treated, among others; by giving local honey, imported honey, povidone iodine (positive control), and negative control group without a treatment. Continued monitoring and recording for the healing process of each group. Statistical analysis is performed by calculation of frequency distribution and Independent-t, Anova, and regression analysis to determine the relationship between each variable. Statistical test results are significant if the value of p <0.05. This research found that addition of honeyaccelerated the wound healing process compared to the administration of water and povidone iodine (p <0.05). This is measured by indicators including; wide wounds, moisture wounds, colour change, and scab changes. By giving honey the perfect wound healing process begins on the 11th day until the 13th day. Besides that, local honey is found to provide healing effects that are faster than imported honey. Giving honey provides a better effect on wound healing. compared with Povidone iodine and without a treatment Keywords: Clove Flower Honey, Manuka Flower Honey, Time of Wasting, Colour of Wound, Moisture

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