scholarly journals Serum and endometrial organic acid profile in women with missed abortion

2019 ◽  
Vol 4 (3) ◽  
pp. 22-26
Author(s):  
I. M. Ordiyants ◽  
S. S. Barabasheva ◽  
A. M. Savicheva
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Protein Separation ◽  
High Performance ◽  
Normal Pregnancy ◽  
Missed Abortion ◽  
Vacuum Aspiration ◽  
Healthy Pregnancy ◽  
Organic Acid Profile ◽  
Hydroxyphenylacetic Acid

Aim. To determine the role of organic acids in the pathogenesis of missing abortion.Materials and Methods. To analyze the endometrial organic acid content, we applied high performance liquid chromatography. Sampling was performed from 20 women with missed abortion and 10 patients with normal pregnancy utilising the vacuum aspiration. Following protein separation by means of double centrifugation and ultrafiltration, supernatant was profiled for the organic acids.Results. Women who experienced missing abortion had significantly higher concentrations of serum and endometrial lactic acid concentrations (10.01 ± 0.16 and 8.28 ± 0.20 mmol/L, respectively) than those with healthy pregnancy (8.16 ± 0.24 and 7.57 ± 0.20 mmol/L, respectively). In contrast, endometrial pyruvic acid level was lower in abortion group (27.71 ± 0.04 mmol/L) as compared to the controls (34.1 ± 0.40 mmol/L). Furthermore, 4-hydroxyphenylacetic acid was found exclusively in women with missed abortion.Conclusion. Changes in organic acid profile may play a major role in pathogenesis of missed abortion. Serum and endometrial 4-hydroxyphenylacetic acid can be suggested as a novel biomarker of early pregnancy loss. 

scholarly journals HPLC FINGERPRINT OF ORGANIC ACIDS IN FRUIT JUICES

1970 ◽  
Vol 62 ◽  
Author(s):  
Loredana Leopold ◽  
Diehl Horst ◽  
Carmen Socaciu
Keyword(s):  
Organic Acids ◽  
Stationary Phase ◽  
Organic Acid ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Fruit Juices ◽  
Hplc Fingerprint ◽  
Organic Acid Profile ◽  
Phase Column ◽  
Reversed Phase Column

Organic acids give fruit products their characteristic tartness and vary in combination and in concentrations among different juices. The organic acid profile can be used to identify a juice or verify its purity. Typically, organic acids in fruit juices are identified and quantified by using methods such as HPLC. In this procedure, reversed phase column is used to separate and identificate six organic acids. Because several of the analytes are extremely difficult to resolve, a aqueous mobile phase is needed to enhance interaction between the acids and the C18 stationary phase.

Occurence of initiation and progression of colorectal cancer in the changed intestinal environment.

2013 ◽  
Vol 31 (4_suppl) ◽  
pp. 416-416
Author(s):  
Seiji Ohigashi ◽  
Kazuki Sudo ◽  
Daiki Kobayashi ◽  
Osamu Takahashi ◽  
Takuya Takahashi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Organic Acids ◽  
Organic Acid ◽  
High Performance ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Intestinal Environment ◽  
Microbial Dysbiosis ◽  
Fecal Ph ◽  
N 93

416 Background: Use of new molecular biology–based methods of bacterial identification is expected to help elucidate the relationship between colorectal cancer (CRC) and intestinal microbiota. However, it remains unclear whether microbial dysbiosis is the cause or the result of CRC onset. We analyzed the intestinal environments to determine whether the changes differed with the stage of CRC or adenoma. Methods: We analyzed fecal microbiota, organic acid concentrations, and pH in CRC patients (n=93), individuals with adenoma (n=23), and individuals with normal intestinal tracts (n=27). After patient hospitalization, the feces of all subjects were collected before any administration of laxatives or antibiotics to prepare the bowel.Thirteen bacterial groups were enumerated in the fecal microbiota by using reverse transcription–quantitative PCR (RT-qPCR). Eight kinds of organic acid were quantified using high-performance liquid chromatography, and fecal pH was measured by pH meter. Results: The counts of total bacteria (10.3 ± 0.7 vs. 10.8 ± 0.3 log10 cells/g of feces; p<0.001), 5 groups of obligate anaerobe (Clostridium coccoides group, C. leptum subgroup, Bacteroides fragilis group, Bifidobacterium, and Atopobium cluster), and 2 groups of facultative anaerobes (Enterobacteriaceae and Staphylococcus) were significantly lower in the CRC group than in the healthy individuals. While the concentrations of organic acids—particularly short chain fatty acids (SCFAs) such as acetic acid, propionic acid, and butyric acid—were significantly decreased in the CRC group, the pH was increased in the CRC group (7.4 ± 0.8 vs. 6.9 ± 0.6; p<0.001). Comparison among the CRC, adenoma, and non-adenoma groups revealed that fecal SCFAs concentrations and pH in the adenoma group were intermediate to the CRC group and the non-adenoma group. Within the CRC group, no differences in microbiota or organic acids were observed among T-stages or Dukes stages. Conclusions: CRC patients showed significant differences in the intestinal environment, including alterations of microbiota, decreased SCFAs, and elevated pH. These changes are not a result of CRC progression but are involved in CRC onset.

scholarly journals COMPOSITION OF MAJOR ORGANIC ACIDS IN VEGETABLES AND SPICES

2015 ◽  
Vol 3 ◽  
pp. 447-454 ◽  
Author(s):  
Liga Priecina ◽  
Daina Karklina
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Acid Content ◽  
High Performance ◽  
Hplc Method ◽  
Metabolic Process ◽  
High Importance ◽  
Taste And Odor ◽  
Pre Treatment ◽  
Organic Acid Content

Organic acids are one of the major phytochemicals in vegetables and responsible for food taste and odor. Different organic acids are analyzed in fruits and cereals, but least in vegetables and spices. Organic acids has been analyzed because of their high importance in the formation of other phytochemical and increased antioxidant activity. The aim of the current research was to determine the oxalic, tartaric, quinic, malic, malonic, ascorbic, citric, fumaric, succinic, salicylic and benzoic acid content in fresh and pre-treated (with steam) vegetables and spices using high performance liquid chromatography (HPLC) method. Major organic acids in highest concentrations in spices and vegetables are quinic, malic, malonic and citric acids. Spices contain higher total organic acid content than vegetables. Using steaming as pre-treatment, some of the organic acids content significantly decreased. Obtained changes could be explained by the organic acid formation into more complex chemicals in food or metabolic process. For the future, these changes will be combined with individual phenolic compound changes in analyzed samples.

Organic acid exudation associated with aluminium stress tolerance in triticale and wheat

2003 ◽  
Vol 54 (10) ◽  
pp. 979 ◽  
Author(s):  
X. G. Zhang ◽  
R. S. Jessop ◽  
D. Alter
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Stress Tolerance ◽  
High Performance ◽  
Root Exudation ◽  
Enzymatic Assay ◽  
Root Tips ◽  
Al Tolerance ◽  
Al Stress ◽  
Organic Acid Exudation

Three triticale cultivars differing in aluminium (Al) stress response, together with 1 Al-tolerant wheat cultivar (Carazinho) and 1 Al-tolerant wheat line (ET3), were used to investigate the root exudation of organic acids during Al stress. The likely relationship of organic acid exudation with Al tolerance, as assessed by root regrowth in nutrient solutions, was also examined. An enzymatic assay was used to detect malate release from both root tips and the whole root system; high performance liquid chromatography (HPLC) was also used to measure the exudation of organic acids from Al-stressed root tips. The enzymatic assay revealed some associations between Al tolerance and malate efflux from Al-stressed wheat or triticale roots, although Al-tolerant triticale cvv. Tahara and 19th ITSN 70-4 released less malate than the Al-tolerant wheat. HPLC analysis indicated that malate and citrate were not the main exudates related to the different levels of Al tolerance in these triticale cultivars. A yet to be identified organic acid in 19th ITSN 70-4 showed significant concentration differences from 2 other cultivars tested. This study highlighted the importance and necessity of elucidating the biochemical mechanisms involved in Al stress tolerance in triticale and other crops.

scholarly journals Organic Acid Profile of “Batuan” (Garcinia binucao [Blco] Choisy) Fruit

2017 ◽  
pp. 25-33 ◽  
Author(s):  
Elizabeth Quevedo ◽  
Erlinda Dizon ◽  
Florinia Merca
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Citric Acid ◽  
Oxalic Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Fumaric Acid ◽  
Mobile Phase ◽  
Fruit Samples ◽  
Organic Acid Profile

“Batuan” fruit (Garcinia binucao [Blco.] Choisy), an indigenous acidulant grown in the Visayas State University, Baybay City, Leyte was analyzed for its organic acid profile at different stages of maturity for the development of potential food and non-food products. The analysis of organic acid content was done using Reverse Phase-High Performance Liquid Chromatography. Organic acids in the dried, powdered “batuan” fruit samples were extracted with the mobile phase (50mM KH2PO4/ H3PO4, pH2.8). The sample extracts and organic acid standards (oxalic acid, tartaric acid, malic acid, citric acid, fumaric acid, lactic acid, acetic acid, and succinic acid) were injected to RP-HPLC under isocratic elution with the mobile phase at a flow rate of 1.0mL min-1 and using UV-vis detection at 210nm. “Batuan” fruit samples contain oxalic acid, tartaric acid, malic acid, citric acid, fumaric acid, succinic acid, acetic acid, lactic acid, and a few unidentified organic acids. Among the organic acids present, citric acid accumulated the highest in the ripe “batuan” fruit; fumaric acid, the least. Results of this study show that “batuan” fruit could be a good natural source of acidulant for food and non-food applications.

scholarly journals Bioleaching Ability of Fungi Isolated from an Indonesian Sulfurous River Sediment

2020 ◽  
Vol 20 (4) ◽  
pp. 810
Author(s):  
Serafica Btari Christiyani Kusumaningrum ◽  
I Wayan Warmada ◽  
Wahyu Wilopo ◽  
Endah Retnaningrum
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
High Performance ◽  
River Sediment ◽  
Low Cost ◽  
Absorption Spectrometry ◽  
Manganese Concentration ◽  
Environmental Damage ◽  
Organic Acid Production ◽  
Phenotypic Characters

The unique characteristics of sulfurous river sediment located in Ungaran, Indonesia, are a reservoir of novel fungi with manganese bioleaching properties. Fungi are known to produce metabolic organic acids that have a potential for the industrial application of leaching metal from the ores. This application has high advantages, including low cost, low energy, and creates minimal environmental damage. Therefore, this research was performed to analyze the manganese bioleaching activities of two fungal isolates (KA2B2 and KB4B) from Indonesian sulfurous river sediment on pyrolusite and determine their phenotypic characters. These activities were investigated in terms of changes in fungal biomass, soluble manganese concentration, pH reduction, and organic acid production during 16 days of leaching. Soluble manganese concentrations were measured by atomic absorption spectrometry (AAS), whereas organic acid concentrations were analyzed by high-performance liquid chromatography (HPLC). According to bioleaching investigations, KA2B2 strain was more efficient than KB4B1 strain in extracting manganese from 0.02 g/cm3 pyrolusite. It also produced higher levels of organic acids, such as oxalic acid and citric acid, than KB4B1 strain, proving that strain of KA2B2 could be used to extract manganese from pyrolusite. Based on the phenotypic characters, both strains were identified as genus Penicillium.

ROLE OF ORGANIC ACIDS AND MORPHO-IMMUNOHISTOCHEMICAL ENDOMETRIAL CHANGES IN PATHOGENESIS OF NON-DEVELOPING PREGNANCY

2020 ◽  
pp. 63-71
Author(s):  
I.M. Ordiyants ◽  
O.S. Pobedinskaya ◽  
S.S. Barabasheva
Keyword(s):  
Organic Acids ◽  
Blood Serum ◽  
High Performance ◽  
Cell Metabolism ◽  
Prognostic Significance ◽  
Krebs Cycle ◽  
Control Group ◽  
Hla Dr ◽  
Hydroxyphenylacetic Acid ◽  
Group 2

Non-developing pregnancy (NDP) is not only a medical, but also a social problem. There is no single predictor of NDP development, its causes and mechanisms are very complex. In recent years, when discussing the genesis and diagnosis of most obstetric complications, special attention has been paid to proteomic and metabolomic factors. Moreover, organic acids have been studied thoroughly, as they are considered to be markers of energy cell metabolism. The objective of the trial was to determine prognostic significance of organic acids in NDP pathogenesis. Material and Methods. We examined 30 women who were divided into 2 groups: Group 1 (main) consisted of 20 women with NDP, Group 2 (control) included women with a physiological pregnancy, seeking for an abortion. All women underwent immunohistochemical study of endometrium. Besides, high performance liquid chromatography (HPLC) was used to identify organic acids in blood serum and endometrium. We analyzed the content of organic acids in blood serum and endometrium, associated with metabolic disorders of fatty acids, amino acids, as well as being intermediate metabolites in the Krebs cycle. STATISTICA® for Windows, Release 6.0 module package from StatSoft® Inc., USA (2003), serial number AXAR802D898511FA, was used for statistical analysis of the obtained data. Results. Women with NDP demonstrated elevated concentrations of lactic acid and decreased concentrations of pyruvic acid in serum and endometrium if compared with the control group (p=0.05). 4-hydroxyphenylacetic acid was found in all women with NDP, while in the control group the very acid was not determined. The revealed changes significantly correlated with immunohistochemical changes in endometrium in women with NDP. We observed high expression of chronic endometritis markers (HLA-DR, CD 16 and CD 20) in the endometrial stroma, decreased expression of VEGF and LIF receptor marker in the epithelium and stroma. Conclusion. Metaboloma indices combined with immunohistochemical characteristics (increased HLA-DR, CD16, CD20 levels, decreased VEGF and LIF expression) can serve as prognostic criteria for prevention of recurrent pregnancy loss. Keywords: non-developing pregnancy, organic acids, metabolites, Krebs cycle. Неразвивающаяся беременность (НБ) – не только медицинская, но и социальная проблема. Нет единого предиктора развития НБ, причины и механизмы очень сложны. В последние годы при обсуждении генеза и диагностики большинства акушерских осложнений особое внимание уделяют протеомным и метаболомным факторам, особенно изучению органических кислот, которые являются маркерами энергетического обмена в клетке. Цель – определить прогностическую значимость органических кислот в патогенезе НБ. Материалы и методы. Обследовано 30 женщин, которые сформировали 2 группы: I основную группу составили 20 женщин с НБ, II контрольную – женщины с физиологически протекающей беременностью, обратившиеся за артифициальным абортом. Всем женщинам проводили иммуногистохимическое исследование эндометрия и определяли органические кислоты в сыворотке крови и эндометрии методом высокоэффективной жидкостной хроматографии. Анализировали содержание органических кислот в сыворотке крови и эндометрии, связанных с нарушениями обмена жирных кислот, аминокислот, а также являющихся промежуточными метаболитами в цикле Кребса. Статистический анализ полученных данных выполняли с помощью пакета модулей STATISTICA® for Windows, Release 6.0 (StatSoft®Inc., США, 2003, серийный номер AXAR802D898511FA). Результаты. У женщин с НБ были выявлены повышенные концентрации молочной и сниженные концентрации пировиноградной кислот в сыворотке крови и эндометрии в сравнении с контрольной группой (p=0,05). У всех женщин с НБ определялась 4-гидроксифенилуксусная кислота, в то время как в группе контроля данная кислота не определялась. Выявленные изменения значимо коррелировали с иммуногистохимическими изменениями в эндометрии у женщин с НБ, где была установлена высокая экспрессия маркеров хронического эндометрита HLA-DR, CD 16 и CD 20 в строме эндометрия и снижение экспрессии VEGF и маркёра рецептивности LIF в эпителии и строме. Выводы. Показатели метаболома в сочетании с иммуногистохимическими особенностями (повышенные уровни экспрессии HLA-DR, CD16, CD20, снижение экспрессии VEGF и LIF) могут служить прогностическими критериями профилактики повторных репродуктивных потерь. Ключевые слова: неразвивающаяся беременность, органические кислоты, метаболиты, цикл Кребса.

scholarly journals ASSESSMENT OF ORGANIC ACID CONTENT, AND BRIX VALUE OF REPRESENTATIVE INDIGENOUS FERMENTED PLANT BEVERAGES OF THAILAND

2016 ◽  
Vol 10 (1) ◽  
pp. 350 ◽  
Author(s):  
Chaiyavat Chaiyasut ◽  
Netnapa Makhamrueang ◽  
Sartjin Peerajan ◽  
Bhagavathi Sundaram Sivamaruthi
Keyword(s):  
Organic Acids ◽  
Organic Acid ◽  
Acid Content ◽  
High Performance ◽  
Ph Value ◽  
Sugar Content ◽  
Human Consumption ◽  
Fermented Foods ◽  
Brix Value ◽  
Organic Acid Content

ABSTRACTObjective: In Thailand, people believe that the fermented plant beverages (FPBs) have the pharmaceutical effects and consumption of FPBs cure thediseases. This study was conducted to detect and quantify the organic acids, pH, and Brix value of the commonly using FPBs of Thailand.Methods: The samples were collected from all the region of Thailand. The total acidity, pH, and organic acid content were measured by titration, pHmeter, and high-performance liquid chromatography methods, respectively. The sugar content of the sample was assessed by Brix value determinationusing a refractometer.Results: All the tested FPBs showed the pH value of 2-4 except sample no. 64 (pH 6.49) and 65 (pH 5.72). The acidity of the samples showed a rangeof ~0.1-2.5% of lactic acid equivalent. The acidity of sample no. 64 and 65 was not in detectable level. As per the Thai Community Product Standard(TCPS) - No. 481/2004, all the samples were qualified for the human consumption except sample no. 64 and 65. About 34, 17, 58, 53, 8, 6, 12, and1 samples were detected with 0.018-0.241, 0.015-0.389, 0.028-1.244, 0.052-0.550, 0.024-0.267, 0.049-0.373, 0.025-0.980, and 0.31 mg/mL of as tartaric,malic, lactic, acetic, citric, sorbic, fumaric, and propionic acids, respectively. The sugar content of the sample no. 9 and 49 was >55 % of Brix value, whileother tested samples were <50%.Conclusion: All the tested FPBs were harmless to human regarding organic acid content, pH, and Brix value except sample no. 64 and 65 as per theTCPS. Further studies are mandatory to explore the microbial and pharmaceutical nature of traditional FPBs and other fermented foods of Thailand.Keywords: Brix value, Fermented plant beverages, Morinda, Organic acids, pH.

scholarly journals Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of Actinidia eriantha ‘Ganmi 6’

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 332
Author(s):  
Zhiqiang Jiang ◽  
Qing Huang ◽  
Dongfeng Jia ◽  
Min Zhong ◽  
Junjie Tao ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
High Performance ◽  
Acid Metabolism ◽  
Citrate Synthase ◽  
Transcriptional Level ◽  
Post Translational Modification ◽  
Shikimate Dehydrogenase ◽  
Organic Acid Metabolism

Studies on organic acid metabolism have been mainly concentrated on the fruit, whereas, few have focused on the mechanism of high organic acids content in the fruit of Actinidia eriantha. Fruits of ‘Ganmi 6’ harvested at eleven developmental periods were used as materials. The components and content of organic acids were determined by high-performance liquid chromatography (HPLC) system, the activities of the related enzyme were detected, and gene expression levels were measured by quantitative real-time PCR (qRT-PCR). Components of ascorbic acid (AsA) and eight kinds of organic acids were detected. These results showed that quinic acid and citric acid were the main organic acids in the fruit of ‘Ganmi 6’. Correlation analysis showed that NADP-Quinate dehydrogenase (NADP-QDH), NADP-Shikimate dehydrogenase (NADP-SDH), and Cyt-Aconitase (Cyt-Aco) may be involved in regulating organic acids biosynthesis. Meanwhile, the SDH gene may play an important role in regulating the accumulation of citric acid. In this study, the activities of NADP-SDH, Mit-Aconitase (Mit-Aco), and NAD-Isocitrate dehydrogenase (NAD-IDH) were regulated by their corresponding genes at the transcriptional level. The activity of Citrate synthase (CS) may be affected by post-translational modification. Our results provided new insight into the characteristics of organic acid metabolism in the fruit of A. eriantha.

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