ROLE OF ORGANIC ACIDS AND MORPHO-IMMUNOHISTOCHEMICAL ENDOMETRIAL CHANGES IN PATHOGENESIS OF NON-DEVELOPING PREGNANCY

2020 ◽  
pp. 63-71
Author(s):  
I.M. Ordiyants ◽  
O.S. Pobedinskaya ◽  
S.S. Barabasheva
Keyword(s):  
Organic Acids ◽  
Blood Serum ◽  
High Performance ◽  
Cell Metabolism ◽  
Prognostic Significance ◽  
Krebs Cycle ◽  
Control Group ◽  
Hla Dr ◽  
Hydroxyphenylacetic Acid ◽  
Group 2

Non-developing pregnancy (NDP) is not only a medical, but also a social problem. There is no single predictor of NDP development, its causes and mechanisms are very complex. In recent years, when discussing the genesis and diagnosis of most obstetric complications, special attention has been paid to proteomic and metabolomic factors. Moreover, organic acids have been studied thoroughly, as they are considered to be markers of energy cell metabolism. The objective of the trial was to determine prognostic significance of organic acids in NDP pathogenesis. Material and Methods. We examined 30 women who were divided into 2 groups: Group 1 (main) consisted of 20 women with NDP, Group 2 (control) included women with a physiological pregnancy, seeking for an abortion. All women underwent immunohistochemical study of endometrium. Besides, high performance liquid chromatography (HPLC) was used to identify organic acids in blood serum and endometrium. We analyzed the content of organic acids in blood serum and endometrium, associated with metabolic disorders of fatty acids, amino acids, as well as being intermediate metabolites in the Krebs cycle. STATISTICA® for Windows, Release 6.0 module package from StatSoft® Inc., USA (2003), serial number AXAR802D898511FA, was used for statistical analysis of the obtained data. Results. Women with NDP demonstrated elevated concentrations of lactic acid and decreased concentrations of pyruvic acid in serum and endometrium if compared with the control group (p=0.05). 4-hydroxyphenylacetic acid was found in all women with NDP, while in the control group the very acid was not determined. The revealed changes significantly correlated with immunohistochemical changes in endometrium in women with NDP. We observed high expression of chronic endometritis markers (HLA-DR, CD 16 and CD 20) in the endometrial stroma, decreased expression of VEGF and LIF receptor marker in the epithelium and stroma. Conclusion. Metaboloma indices combined with immunohistochemical characteristics (increased HLA-DR, CD16, CD20 levels, decreased VEGF and LIF expression) can serve as prognostic criteria for prevention of recurrent pregnancy loss. Keywords: non-developing pregnancy, organic acids, metabolites, Krebs cycle. Неразвивающаяся беременность (НБ) – не только медицинская, но и социальная проблема. Нет единого предиктора развития НБ, причины и механизмы очень сложны. В последние годы при обсуждении генеза и диагностики большинства акушерских осложнений особое внимание уделяют протеомным и метаболомным факторам, особенно изучению органических кислот, которые являются маркерами энергетического обмена в клетке. Цель – определить прогностическую значимость органических кислот в патогенезе НБ. Материалы и методы. Обследовано 30 женщин, которые сформировали 2 группы: I основную группу составили 20 женщин с НБ, II контрольную – женщины с физиологически протекающей беременностью, обратившиеся за артифициальным абортом. Всем женщинам проводили иммуногистохимическое исследование эндометрия и определяли органические кислоты в сыворотке крови и эндометрии методом высокоэффективной жидкостной хроматографии. Анализировали содержание органических кислот в сыворотке крови и эндометрии, связанных с нарушениями обмена жирных кислот, аминокислот, а также являющихся промежуточными метаболитами в цикле Кребса. Статистический анализ полученных данных выполняли с помощью пакета модулей STATISTICA® for Windows, Release 6.0 (StatSoft®Inc., США, 2003, серийный номер AXAR802D898511FA). Результаты. У женщин с НБ были выявлены повышенные концентрации молочной и сниженные концентрации пировиноградной кислот в сыворотке крови и эндометрии в сравнении с контрольной группой (p=0,05). У всех женщин с НБ определялась 4-гидроксифенилуксусная кислота, в то время как в группе контроля данная кислота не определялась. Выявленные изменения значимо коррелировали с иммуногистохимическими изменениями в эндометрии у женщин с НБ, где была установлена высокая экспрессия маркеров хронического эндометрита HLA-DR, CD 16 и CD 20 в строме эндометрия и снижение экспрессии VEGF и маркёра рецептивности LIF в эпителии и строме. Выводы. Показатели метаболома в сочетании с иммуногистохимическими особенностями (повышенные уровни экспрессии HLA-DR, CD16, CD20, снижение экспрессии VEGF и LIF) могут служить прогностическими критериями профилактики повторных репродуктивных потерь. Ключевые слова: неразвивающаяся беременность, органические кислоты, метаболиты, цикл Кребса.

scholarly journals Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

2016 ◽  
Vol 77 (2) ◽  
pp. 304-311 ◽  
Author(s):  
L. D. Silva ◽  
V. C. S. Amaral ◽  
M. C. Vinaud ◽  
A. M. Castro ◽  
H. H. A. Rezende ◽  
...  
Keyword(s):  
Organic Acids ◽  
High Performance ◽  
Chemical Elements ◽  
Essential Element ◽  
Biomphalaria Glabrata ◽  
Control Group ◽  
Limiting Factor ◽  
Energetic Metabolism ◽  
Significant Difference ◽  
Commercial Kits

Abstract Calcium is considered an essential element for the metabolism of aquatic snail Biomphalaria glabrata (Say, 1818), intermediate host of Schistosoma mansoni Sambon, 1907 in Brazil, and represents a limiting factor to its distribution and adaptation to the environment. This study investigated the effect of different concentrations of exogenous CaCO3 on the energetic metabolism of B. glabrata for better understanding the physiological interference of chemical elements dissolved in the environment with the physiology of this species. Sixty-day-old snails were distributed into six groups, five exposed to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) and a control group. The exposure to CaCO3 was assessed over time, with analysis of 15 snails of each group in the following intervals: 1, 14, 21 or 30 days for hemolymph extraction. Concentrations of calcium and glucose in the hemolymph were determined by commercial kits, and organic acids were extracted using an ion exchange column and analyzed by high-performance liquid chromatography. Concentration of calcium in the hemolymph showed no significant difference (p>0.05) from the control group and between the concentrations tested. Concentration of glucose decreased (p<0.05) in the treatments of exposure to 20 and 40 mg/L and increased when exposed to 80 and 100 mg/L CaCO3 compared to control and to other concentrations tested over 30 days. The organic acids pyruvate, oxaloacetate, citrate, succinate, fumarate, beta-hydroxybutyrate and lactate presented increased concentrations, while propionate and acetoacetate, decreased concentrations, when exposed to CaCO3 compared to control. Considering the influence of different periods of exposure to CaCO3, on the 14th day, there were stronger alterations in the metabolism of B. glabrata. In conclusion, exposure to CaCO3 reduced the concentration of glucose, which is metabolized into pyruvate, the final product of glycolysis, and also influenced the energetic metabolism pathways, indicating an aerobic or partially anaerobic functioning.

DIAGNOSTIC SIGNIFICANCE OF BLOOD SERUM CYTOKINE PROFILE FOR CHRONIC KIDNEY DISEASE IN CHILDREN

2018 ◽  
Vol 22 (4) ◽  
pp. 81-89
Author(s):  
O. V. Semeshina ◽  
V. N. Luchaninova ◽  
A. Nee ◽  
E. V. Markelova ◽  
N. V. Gorelik ◽  
...  
Keyword(s):  
Blood Serum ◽  
Kidney Diseases ◽  
Prognostic Significance ◽  
Control Group ◽  
Specific Marker ◽  
Healthy Children ◽  
Serum Cytokine ◽  
Diagnostic Significance ◽  
Tnf Α ◽  
Tgf Β1

THE AIM: to study diagnostic and prognostic significance of blood serum cytokine status evaluation in children with different nosological forms of kidney diseases.PATIENTS AND METHODS. The study included 255 children with various kidney diseases (kidney stone disease (KSD) – 16, with  infectious kidney diseases (IKD) – 174, with a glomerulopathy (GP)  – 65). In all study groups were dominant children with 1st and 2nd  stage of CKD (100%, 97,5% and 95,4% respectively). The control group included 50 virtually healthy children. All patients  determined level of TNF-α, TNF-RI and TNF-RII, IL-10, TGF-β1 and TGF-β3, IL2, IL2-SR in blood serum.RESULTS.Increase of TNF-α level in blood serum can be considered as a highly specific marker of acute pielonephritis chronization, as  well as decrease of TNF-RII concentration can be considered as a  marker of full clinical and laboratory pielonephritis remission. The  increase in TNF-α and TNF-RI can also be considered as a marker of autoimmune inflammation. Deficiency of IL-2, IL-10 and TGF-β3  with an increase in IL-2 R in blood should be used as a marker of  bacterial-inflammatory and autoimmune kidney diseases, and TGF- β1 increase as an early marker of nephrosclerosis, especially in  patients with glomerulonephritis. The increase of the inflammatory  index TNF-α / IL-10 more than 4 times, gives us  the opportunity to  position it as an additional diagnostic criterion for infectious and  autoimmune process in the kidneys. The increase in urinary  excretion of TNF-α with the decrease of IL-10 by maintaining  consistently high concentrations of TGF-β1 is a marker of  inflammation and fibrosis in infectious kidney diseases and  glomerulonephritis. Modern nephroprotection therapy directed at  slowing progression of CKD and its complications should include  modulation of cytokine status.

Clinical and prognostic significance of the soluble form of the VISTA immunity control point in patients with primary bone tumors

2021 ◽  
Vol 66 (9) ◽  
pp. 533-538
Author(s):  
N. E. Kushlinskii ◽  
Olga Vladimirovna Kovaleva ◽  
Yu. B. Kuzmin ◽  
E. A. Korotkova ◽  
E. S. Gershtein ◽  
...  
Keyword(s):  
Blood Serum ◽  
Bone Tumors ◽  
Control Point ◽  
Prognostic Significance ◽  
Bone Neoplasms ◽  
Soluble Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Malignant Neoplasms ◽  
Healthy Donors

The data of a comparative enzyme-linked immunosorbent assay of the content of the soluble form of the immunity checkpoint VISTA in the blood serum of 30 healthy donors (control group), 79 patients with primary malignant (osteosarcoma - 30, chondrosarcoma - 31, chordoma - 14) and 14 borderline (giant cell tumor) bone neoplasms are presented. In the general group of patients with malignant neoplasms of bones, the median sVISTA content in blood serum is statistically significant lower than in the control (p = 0.040). In patients with bone tumors and healthy donors over 18 years of age, there was a decrease with age in serum sVISTA levels. There were no significant differences in sVISTA concentration between patients with osteosarcoma, chondrosarcoma and healthy donors. Only in patients with chordoma were sVISTA levels statistically significant lower than in controls (p = 0.013). In the groups of patients with chondrosarcoma and osteosarcoma of the bone, there were no significant associations between the serum sVISTA content and the main clinical and morphological characteristics of the disease. In patients with osteosarcoma, no relationship was found between sVISTA levels and overall survival rates, while in patients with bone chondrosarcoma, there was a tendency towards a favorable prognosis with a high content of the marker in the blood serum.

scholarly journals Influence of cadmium sulfate at different doses on the functional state of the liver of laying chicken

2019 ◽  
Vol 21 (94) ◽  
pp. 103-108 ◽  
Author(s):  
A. Y. Ostapyuk ◽  
B. V. Gutyj
Keyword(s):  
Body Weight ◽  
Blood Serum ◽  
Functional State ◽  
The Body ◽  
Control Group ◽  
Cadmium Sulfate ◽  
Group 4 ◽  
Group 2 ◽  
Destructive Processes ◽  
Different Doses

The article presents the results of studies on the influence of cadmium sulfate in different doses on the organism of poultry. Absorbed cadmium accumulates in the liver as a complex with metallothionein. The purpose of the work was to find out the effect of cadmium sulfate in doses of 2 and 4 mg / kg of body weight on the functional state of the liver of chicken. For research, three groups of birds were formed: control and two experimental. The control group of chickens were in the usual diet, they were fed with forage and were given water without introducing cadmium sulfate. To the drinking water of chickens of experimental groups for 30 days, added cadmium sulfate in doses: the first group – 2 mg/kg, the second group – 4 mg/kg body weight. The conditions for keeping chickens and the microclimate parameters in the room for all bird groups were similar. Blood from the chickens was taken from the subclavian vein in periods: before the dasg was given and on the first, seventh, fourteenth, twenty-first and thirtieth day of the experiment. It was determined that the presentation of cadmium sulfate in doses of 2 and 4 mg/kg of body weight of cows, which contributed to a violation of the functional state of the liver, as evidenced by the increased activity of aminotransferases in their blood serum. The activity of alanine and aspartate aminotransferase was higher in serum of blood of chickens in the second experimental group at 21st and 30th days of the experiment. The high activity of aminotransferases in the blood serum of chickens for cadmium loading indicates destructive processes in the liver that cause an increase in the release of aminotransaminases from cellular organelles in the blood of experimental poultry. Thus, the obtained results indicate an increase in destructive processes in the body of chickens for cadmium loading.

Activity of thymidine kinase as a prognostic marker of malignant proliferation in adolescents with lymphadenopathy.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e19013-e19013
Author(s):  
Viktoria V. Dmitrieva ◽  
Elena M. Frantsiyants ◽  
Irina B. Lysenko ◽  
Elena E. Pak ◽  
Olga V. Kozyuk ◽  
...  
Keyword(s):  
Blood Serum ◽  
Thymidine Kinase ◽  
Hodgkin’S Lymphoma ◽  
Prognostic Significance ◽  
Elevated Serum ◽  
Test System ◽  
Hodgkin's Lymphoma ◽  
Control Group ◽  
Malignant Cells ◽  
Intracellular Enzyme

e19013 Background: Lymphadenopathy presents an important differential diagnostic problem. Thymidine kinase (TK) is an intracellular enzyme catalyzing the conversion of thymidine to thymidine monophosphate in the presence of adenosine triphosphate. TK is the product of certain genes for the progression from G1 through S phase. Determination of serum levels of the first TK isoenzyme is considered to have prognostic significance in malignant proliferation. TK increases significantly in the direct contact of malignant cells with biological fluids such as blood, lymph, or serous effusions, therefore its level changes most significantly in systemic blood diseases. Elevated serum TK has prognostic information and determines a high risk of tumor progression. The purpose of the study was to improve the accuracy of diagnosis of Hodgkin's lymphoma in adolescents, with its differentiation from lymphadenitis. Methods: The study included 34 patients: 13 – lymphadenitis, 21 – Hodgkin's lymphoma. Healthy donors served as a control group. Activity of TK1 (U/L) was determined in the blood serum by radioenzymatic method (in vitro) using a standard test system (Immunotech, BeckmanCoulter, Czech Republic). When TK1 activity was within 10.6-14.8 U/L, adenopathy of unclear etiology was considered lymphadenitis, and in 39.6-45 U/L – Hodgkin's lymphoma. Results: Activity of TK in the blood serum of patients with lymphadenitis (U/L) is shown in Table. Conclusions: Activity of thymidine kinase 1 can be measured as an objective marker for the proliferation of malignant cells, increasing the accuracy of Hodgkin's lymphoma diagnosis in adolescents.[Table: see text]

scholarly journals The Effect of Recently Developed Synbiotic Preparations on Dominant Fecal Microbiota and Organic Acids Concentrations in Feces of Piglets from Nursing to Fattening

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1999
Author(s):  
Agnieszka Chlebicz-Wójcik ◽  
Katarzyna Śliżewska
Keyword(s):  
Fatty Acids ◽  
Organic Acids ◽  
Intestinal Microbiota ◽  
High Performance ◽  
Negative Impact ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Plate Count ◽  
Control Group ◽  
Chain Fatty Acids

The study was conducted to determine the influence of newly elaborated synbiotic preparations on piglets’ intestinal microbiota and its metabolism. Animals were distributed among six experimental groups, in reference to used feed supplements, namely, synbiotics (A, B, or C) or commercially available probiotics (BioPlus 2B®, Chr. Hansen A/S, Horsholm, Denmark or Cylactin® LBC, DSM Nutritional Products Ltd., Kaiseraugst, Switzerland), or its absence (control group). Until the 29th day of life, piglets were breastfed by sows, whose feed was supplemented, and fecal samples were collected at the 7th and 28th day of piglets’ life. After weaning of the piglets, the research was continued until the 165th day of the pigs’ life. The area of this work included the analysis of the piglets’ dominant fecal microbiota by the plate count method. Moreover, high-performance liquid chromatography analysis (HPLC) was applied to establish variations in the concentrations of organic acids, namely, lactic acid, short-chain fatty acids (SCFAs), and branched-chain fatty acids (BCFAs). It was observed that synbiotics have a more significant beneficial effect on the intestinal microbiota of piglets and their metabolism, and therefore their health, in comparison to commercial probiotics used individually. Moreover, synbiotic preparations prevent the negative impact of weaning on piglets’ microbial population in the gastrointestinal tract, which could reduce the occurrence of diarrhea.

scholarly journals The metabolic syndrome as a risk factor for colorectal cancer

2017 ◽  
Vol 14 (2) ◽  
pp. 24-32 ◽  
Author(s):  
Tatiana M. Nikitenko ◽  
Lilia V. Shcherbakova ◽  
Sofia K. Malyutina ◽  
Svetlana V. Mustafina ◽  
Eugene G. Verevkin ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metabolic Syndrome ◽  
Antihypertensive Treatment ◽  
Prognostic Significance ◽  
Negative Relationship ◽  
Control Group ◽  
Glucose Levels ◽  
The Metabolic Syndrome ◽  
Elevated Glucose ◽  
Group 2

Objective. To evaluate the prognostic significance of metabolic syndrome (MS) in the development of colorectal cancer (CRC) using various MS criteria in Novosibirsk population. Materials and Methods. The study was designed as nested case-control. Baseline population cohort (9360 men, women aged 4569) was examined in the HAPIEE project and followed-up during 11 years. The cases included all subjects, who had CRC during 11-year follow-up according to the Register of Cancer (n=99, M-52, M-47). The matched control group (2/1) was selected from HAPIEE cohort (n=198, m-104, w-94). The prospective study of CRC was supported by RSF. MS criteria were determined in accordance to VNOK (2009), IDF (2005), NCEP ATP III (2001). Statistical package SPSS v.11.0 was used. Logistic regression was used to estimate the association between MS and risk of CRC. Results. Women with glucose levels 6.1 mmol/l had 3 times higher 11-year risk of CRC then those with glucose 6.1 mmol/l (OR=3.11; 95%CI:1.237.87, VNOK, 2009; OR=3.20; 95%CI:1.278.08, NCEP ATP III, 2001). Blood pressure (BP) 130/85 mmHg was associated with decreased risk of CRC in women and in both sexes, but the relationship became insignificant after controlling for antihypertensive treatment. Other components of the MS were not significantly associated with CRC risk. Conclusions. In studied sample the 11-year risk of developing CRC was significantly increased in women with elevated glucose levels. The negative relationship between elevated BP and the risk of CRC in women and both sexes became insignificant when adjusted for antihypertensive treatment; this finding requires further exploration.

scholarly journals High-performance inulin and oligofructose prebiotics increase the intestinal absorption of iron in rats with iron deficiency anaemia during the growth phase

2011 ◽  
Vol 108 (6) ◽  
pp. 1008-1016 ◽  
Author(s):  
Karine de Cássia Freitas ◽  
Olga Maria Silvério Amancio ◽  
Mauro Batista de Morais
Keyword(s):  
Intestinal Absorption ◽  
High Frequency ◽  
High Performance ◽  
Fe Deficiency ◽  
Control Group ◽  
Control Groups ◽  
Deficiency Anaemia ◽  
Significant Difference ◽  
Group 2 ◽  
And Control

Considering the high frequency of anaemia due to Fe deficiency, it is important to evaluate the effects of prebiotics on the absorption of Fe. The aim of the present study was to evaluate the effects of high-performance (HP) inulin, oligofructose and synergy1 during recovery from anaemia in rats through the intestinal absorption of Fe, food intake, body growth, caecal pH and weight of the intestine. Wistar rats (n 47) were fed with rations of AIN93-G with no Fe to induce Fe deficiency anaemia. At 36 d of life, anaemic rats were divided into four groups: (1) the HP inulin group; (2) the synergy1 group; and (3) the oligofructose group, all with 100 g of the respective prebiotic per kg of ration; and (4) a control group, in which the prebiotic was replaced by maize starch. Then, 25 mg of elemental Fe/kg of ration was added to all rations to allow recovery from anaemia. The final values of Hb in the HP inulin, synergy1, oligofructose and control groups were, respectively: 98 (94–99); 83 (81–92); 100 (90–114); 77 (72–81) g/l, with a statistically significant difference (P ≤ 0·001) between the oligofructose and control groups and the HP inulin and control groups. The four groups had an increase in weight and body length and had similar consumption of rations. The intestinal weight and caecal pH were significantly different between the groups that consumed prebiotics and the control group. HP inulin and oligofructose increased the intestinal absorption of Fe in rats.

scholarly journals The effects of dried grape pomace supplementation on biochemical blood serum indicators and digestibility of nutrients in horses

2020 ◽  
Vol 65 (No. 2) ◽  
pp. 58-65
Author(s):  
Renata Kolláthová ◽  
Branislav Gálik ◽  
Marko Halo ◽  
Anton Kováčik ◽  
Ondrej Hanušovský ◽  
...  
Keyword(s):  
Blood Serum ◽  
Grape Pomace ◽  
Control Group ◽  
Acid Detergent Fibre ◽  
Negative Effect ◽  
Serum Biochemical ◽  
Group 2 ◽  
Experimental Group ◽  
Group 1

Twelve adult Slovak warmblood sport horses were used to study the effect of dried grape pomace (DGP) on health through blood serum biochemical indicators, and on apparent total tract digestibility of dry matter (DM), organic matter (OM), crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF). The digestibility analysis was carried out by two in vivo methods, total faeces collection (TFC) and using lignin as a marker (ADL). Animals were divided into 3 groups: control group (C, without supplementation), experimental group 1 (E1, feed rations + 200 g of DGP) and experimental group 2 (E2, feed rations + 400 g of DGP). In animals, no health problems were detected during the trial. Of the blood serum indicators, only the concentrations of potassium (increase in E2 group compared to C group) and alanine aminotransferase (decrease in E2 group in comparison with E1 and C group) were affected (P &lt; 0.05). The ADL method resulted in underestimated digestibility coefficients due to low recovery rates of lignin (less than 90%) in C group and E1 group. According to TFC, in E1 group higher digestibility coefficients were detected for DM, OM and CP (P &gt; 0.05) compared to C group. However, in E2 group lower digestibility of all the studied nutrients was found (P &gt; 0.05) in comparison with C group and E1 group. These results suggest that DGP could be used in horse diets up to 200 g without negative effect on their health and for a possible digestibility improvement of some nutrients.

INFLUENCE OF THE DURABLE APPLICATION OF PROTONIC PUMP INHIBITORS ON CYTOKINE CONCENTRATION IN RAT BLOOD SERUM

2021 ◽  
Vol 74 (7) ◽  
pp. 1575-1580
Author(s):  
Serhii V. Pylypenko ◽  
Andrii A. Koval ◽  
Makarchuk V. Viktoria ◽  
Kostiantyn F. Chub
Keyword(s):  
Blood Serum ◽  
Inflammatory Cytokines ◽  
Male Rats ◽  
Control Group ◽  
Hcl Secretion ◽  
Group 3 ◽  
Anti Inflammatory ◽  
Group 2 ◽  
Student’S T ◽  
Group 1

The aim: Of the work was to determine the content of pro- and anti-inflammatory cytokines in the blood serum of the control group rats and after 28 days of inhibiting HCl secretion in the stomach by proton pump blockers “Omeprazole” and “Pantoprazole”. Materials and methods: The studies were performed on 30 white non-linear male rats weighing 160-180 g, divided into three groups with 10 animals in each. The control (group 1) were injected intraperitoneally with water for injections within 28 days once a day. Group 2 was administered omeprazole. Group 3 was administered pantoprazole. The concentration of cytokines in the blood serum of rats was determined by the enzyme immunoassay method. For statistic data processing, Student’s t-criterion for independent samples was applied. Results: After prolonged administration of omeprazole and pantoprazole, the blood serum concentrations of IFNγ, TNFα, IL-1 in rats increased by 58.5% and 3.41%, 73.3% and 48.4%, 80.2% and 40.8%, respectively, and IL-12B 40p decreased by 36.6% when using omeprazole and was almost indistinguishable from the control values when pantoprazole was administered. With administration of omeprazole, IL-4 concentration decreased by 39.8% and that of pantoprazole increased by 3.86% compared to the control. Administration of omeprazole and pantoprazole did not affect IL-6 concentration. Conclusion: Inhibition of hydrochloric acid secretion in the stomach of rats for 28 days using omeprazole and pantoprazole led to an imbalance between pro- and anti-inflammatory cytokines. The adverse effect of pantoprazole was less pronounced than that of omeprazole.

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