scholarly journals Various ORSV infection symptoms on ceratorhiza induction in orchid plants

2021 ◽  
Vol 1 (2) ◽  
pp. 64-71
Author(s):  
Mitha Valentina Treesya Panjaitan ◽  
Mahfut Mahfut ◽  
Tundjung Tripeni Handayani ◽  
Sri Wahyuningsih
Keyword(s):  
Life Cycle ◽  
Host Plant ◽  
Root System ◽  
Root Length ◽  
Leaf Length ◽  
Disease Symptoms ◽  
Odontoglossum Ringspot Virus ◽  
Randomized Design ◽  
Length Width ◽  
Completely Randomized Design

Several types of orchids that are in great demand and cultivated by various groups are Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are needed by orchid plants to complete their life cycle. This is because mycorrhizae are capable of symbiosis with the root system of the host plant so that the plant's capacity to absorb elements will increase. This study aims to determine the extent to which Ceratorhiza induction on Phalaenopsis amabilis and Dendrobium discolour orchids can suppress the symptoms of Odontoglossum ringspot virus (ORSV) infection. The method used in this study was a factorial completely randomized design (CRD) with 2 factors and 4 replications. The first factor is the type of orchid used, namely Phalaenopsis amabilis (A1) and Dendrobium discolour (A2), while the second factor is the type of administration of mycorrhizae, viruses, and mycorrhizal viruses. Observation of disease symptoms is done by looking at the symptoms that appear on leaves that have been infected with ORSV. The results showed that the symptoms that appeared in Phalaenopsis amabilis were in the form of mosaic, necrosis, leaf malformation, while in Dendrobium discolour were mosaic and necrotic. Ceratorhiza was not able to suppress the symptoms of disease infection because based on the results obtained; the treatment on orchids inoculated with mycorrhizae and viruses showed more severe symptoms compared to the treatment only inoculated with viruses increased response and Dendrobium discolours showed a decreased response in leaf length, width, and number. Meanwhile, in the root organ, Dendrobium discolour showed an increased response and Phalaenopsis amabilis showed a decreased response in root length and number of roots.

scholarly journals Desenvolvimento do capim vetiver cultivado em diferentes ambientes e adubado com esterco bovino

2020 ◽  
Vol 1 (50) ◽  
pp. 130
Author(s):  
Leonardo Oliveira Santos Medeiros ◽  
Maria Teresa Cristina Coelho Do Nascimento ◽  
Joelma Sales dos Dos Santos ◽  
Ilza Maria do Nascimento Brasileiro ◽  
Carlos Alberto Vieira de Azevedo
Keyword(s):  
Root System ◽  
Plant Height ◽  
Root Length ◽  
Aerial Part ◽  
Dry Mass ◽  
Vetiver Grass ◽  
Ambient Conditions ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Free Environment

<p>The objective of this work was to evaluate the development of vetiver grass (Chrysopogon zizanioides L. Roberty) cultivated in PVC columns and fertilized with bovine manure levels under different ambient conditions. The experiment was installed in a completely randomized design, with three replications, in a factorial scheme 5 x 2 being five levels of bovine manure (0%, 10%, 20%, 30% and 40%) and two types of environments (free and protected environment with 80% opening shade), totalizing 30 experimental units. Evaluations were carried out regarding plant height and number of shoots to 15, 30, 45 and 60 days after transplanting (DAT). At 60 DAT at the end of the experiment, the plants were separated to determine the root length and the production of green and dry mass of the aerial part and the root. The results obtained showed that the absence of bovine manure contributed to the greater development of the root system of vetiver grass plants. The 10% level of bovine manure provided a greater number of sprouts and increase in the green mass of the aerial part and green dry mass of the root. The free environment provided the greatest development of vetiver grass plants.</p>

scholarly journals Pemilihan Bagian Eksplan pada Stek Batang Tanaman Sambung Nyawa

2020 ◽  
Vol 20 (2) ◽  
pp. 173
Author(s):  
Ratna Dewi Eskundari ◽  
Nur Rohimah Hanik
Keyword(s):  
Human Health ◽  
Root Length ◽  
Leaf Length ◽  
Shoot Length ◽  
Leaf Width ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Anti Inflammatory ◽  
Different Parts ◽  
Selection Of

Abstrak: Tanaman sambung nyawa (Gynura procumbens) dikenal sebagai salah satu tanaman yang bermanfaat bagi kesehatan tubuh. Daun tanaman ini diketahui mempunyai efek anti peradangan yang dapat mengurangi bahkan mengobati berbagai macam penyakit. Penelitian ini berfokus pada pemilihan bagian batang tanaman sambung nyawa yang mempunyai kapasitas terbaik sebagai eksplan stek. Penelitian ini dilakukan dengan menanam 3 bagian batang tanaman sambung nyawa yang berbeda, yaitu bagian atas (pucuk sampai dengan 10 cm ke bawah), tengah (bagian batang yang berjarak 10cm dari pucuk sampai 20 cm), dan bawah (bagian batang yang berjarak 20 cm dari pucuk hingga 30 cm). Ketiga jenis eksplan ini ditanam di tanah yang mengandung humus tanpa diberi perlakuan penambahan ZPT. Penelitian ini menggunakan rancangan acak lengkap dengan sepuluh ulangan. Hasil penelitian menunjukkan bahwa tidak ada perbedaan yang nyata antara bagian batang dengan panjang daun, tetapi terdapat perbedaan yang nyata dengan lebar daun, panjang tunas, dan panjang akar. Hasil penelitian ini diharapkan dapat menjadi rujukan bagian batang “tengah” dan atau “bawah” sebagai eksplan terbaik dalam usaha perbanyakan tanaman sambung nyawa menggunakan stek. Kata kunci: organogenesis, perbanyakan, sambung nyawa, stek.Abstract: Sambung nyawa plant (Gynura procumbens) is known as one of the plants that are beneficial for human health. The leaves of this plant are known to have anti-inflammatory effects that can reduce and even treat various diseases. This research focused on the selection of the stem part of sambung nyawa plant which has the best capacity as explant cuttings. This research was carried out by planting three different parts of the stem of the plant, namely the upper part (shoots apical up to 10 cm down part), the middle (the stem part that is 10cm from the top to 20 cm down part), and the bottom (the part of the stem which is 20 cm apart from shoots up to 30 cm down part). These three types of explants were planted in soil containing humus without being treated with the addition of PGR(s). This study used a completely randomized design with ten replications. The results showed that there was no significant differences between the stem parts with leaf length parameter, but there were significant differences in leaf width, shoot length, and root length ones. The results of this study were expected to be a reference part of the stem "middle" and or "bottom" as the best explant in the effort to propagate the sambung nyawa plants using cuttings.Keywords: organogenesis, propagation, sambung nyawa, cuttings. 

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

scholarly journals Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. N. Mahadi ◽  
F. Zawawi ◽  
R. Nulit ◽  
M. H. Ibrahim ◽  
N. I. Ab. Ghani
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Germination Percentage ◽  
Leaf Length ◽  
Seed Vigor ◽  
Rice Seed ◽  
Combination Treatments ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

scholarly journals ALTERNATIVE SUBSTRATES FOR PRODUCTION OF YELLOW PASSION FRUIT SEEDLINGS

2020 ◽  
Vol 7 (1) ◽  
pp. 43-48
Author(s):  
Eduardo Santana Aires ◽  
Carlos Alberto Aragão ◽  
Itala Laiane Silva Gomes ◽  
Gilmário Noberto de Souza ◽  
Isa Gabriela Vieira de Andrade
Keyword(s):  
Plant Height ◽  
Dry Matter ◽  
Passion Fruit ◽  
Dry Matter Accumulation ◽  
Alternative Substrates ◽  
Randomized Design ◽  
Length Width ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Yellow Passion Fruit

This study aimed to evaluate different substrates to produce yellow passion fruit seedlings. For this purpose, an experiment was carried out in a greenhouse, from April to June 2016. A completely randomized design with five replications, in a 6 x 5 factorial scheme was used. The first factor was the alternative substrates (poultry, cattle and equine manure, sugarcane and coconut bagasse, and Organomais compost). The second factor was the replacement levels to the commercial substrate of Plantmax® (100:0, 75:25, 50:50, 25:75, 0:100). The seedling evaluation was carried out 42 days after sowing. The following variables were analyzed: length, width, and the number of leaves; plant height and shoot dry matter. A significant effect (P0.05) for the interaction between the factors was verified. The addition of 60% cattle manure and Organomais to the commercial substrate Plantmax® promoted greater length, width, and the number of leaves, plant height, and shoot dry matter accumulation in the yellow passion fruit seedlings.

scholarly journals In vitro development of Encyclea cordigera in different concentrations of honey

2015 ◽  
Vol 46 (4) ◽  
pp. 590-592 ◽  
Author(s):  
Cibele Mantovani ◽  
Kathia Fernandes Lopes Pivetta
Keyword(s):  
Culture Media ◽  
Leaf Length ◽  
In Vitro Development ◽  
Randomized Design ◽  
Sucrose Medium ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Set Up ◽  
Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

scholarly journals Utilização de diferentes substratos na propagação de Pyrostegia venusta através de estacas

2015 ◽  
Vol 10 (4) ◽  
pp. 38
Author(s):  
J. G. Crispim ◽  
M. M. Rêgo ◽  
E. R. Rêgo ◽  
Â. M. S. Pessoa ◽  
P. A. Barroso
Keyword(s):  
Analysis Of Variance ◽  
Leaf Length ◽  
Cattle Manure ◽  
Main Root ◽  
Plastic Tube ◽  
Tukey Test ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Pyrostegia Venusta

<p>Este trabalho teve por objetivo avaliar o desenvolvimento de clones de <em>Pyrostegia venusta </em>em diferentes tipos de substratos. O experimento foi conduzido em delineamento inteiramente casualizado, com 06 tratamentos e 10 repetições, cada repetição foi constituída por um tubete de polietileno com capacidade de 103cm<sup>3</sup>. Os tratamentos testados foram: (T1) 100% de areia lavada; (T2) 50% areia lavada + 50% substrato comercial Plantmax®; (T3) 100% de substrato comercial Plantmax®; (T4) 50% areia lavada + 50% de esterco bovino; (T5) 1/3 areia lavada + 1/3 esterco bovino +1/3 de Plantmax® e (T6) 50% esterco bovino + 50% de Areia lavada. Após 30 dias foram avaliados o número de estacas sobreviventes, comprimento do broto (cm), número de folhas, comprimento da folha (cm), número de raízes e comprimento da raiz principal (cm).  Os dados foram submetidos a análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Por meio da análise de variância (p&lt;0,01), detectou-se significância estatística, para as variáveis, sobrevivência das estacas e número de brotos por estaca de <em>P. venusta</em>, quando cultivadas em substrato composto por 50% areia lavada + 50% substrato comercial Plantmax®. Essa mistura apresenta a vantagem de ser mais econômica quando comparado ao substrato comercial Plantmax®, sendo portanto, recomendada na produção de mudas de <em>cipó-de-São João</em><em>.</em></p><p align="center"><strong><em>Utilization of different substrates in the propagation of Pyrostegia venusta by cuttings</em></strong></p><p><strong>Abstract</strong><strong>: </strong>The experiment was carried out in a completely randomized design with 06 treatments and 10 replicates, each replicate consisted of polyethylene plastic tube the size of 103cm<sup>3</sup>. The treatments were: (T1) 100% washed sand; (T2) 50% washed sand + 50% commercial substrate Plantmax®; (T3) 100% substrate commercial Plantmax®; (T4) 50 % washed sand + 50% cattle manure; (T5) 1/3 washed sand + 1/3 manure +1/3 Plantmax® and Plantmax (T6) 50 % cattle manure + 50% washed sand. After 30 days, were evaluated number of cutting survival, bud length in cm, number of leaves, leaf length in cm, number of roots and length of the main root. Data were submitted to analysis of variance and means were compared by Tukey test at 5% probability. By analysis of variance (p&lt; 0.01), there was significant differences for the variables, czutting survival and number of shoots per cutting when cultured on substrate composed of 50% washed sand + 50% commercial substrate Plantmax®. Furthermore, this mixture has the advantage to be cheaper as compared to substrate Plantmax®, so we recommend the seedlings of Saint John’s vine.</p><p><em><br /></em></p>

scholarly journals Biochar as an alternative substrate for the production of sugarcane seedlings

2021 ◽  
Vol 25 (12) ◽  
pp. 826-832
Author(s):  
Mauro F. C. Mota ◽  
Eulina F. Damião ◽  
Matheus R. Torres ◽  
Rodinei F. Pegoraro ◽  
Leidivan A. Frazão ◽  
...  
Keyword(s):  
Particle Size ◽  
Plant Height ◽  
Dry Mass ◽  
Leaf Length ◽  
Particle Sizes ◽  
Alternative Substrate ◽  
Aerial Parts ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Important Form

ABSTRACT Biochar, which has emerged as an important form of the transformation and final disposal of biomass, can be used directly in soil or in seedling nurseries. In this study, the use of biochar of different particle sizes and percentages was evaluated in replacement to a conventional substrate used in the production of sugarcane seedlings. To this end, an experiment was carried out based on a completely randomized design, with a 5 × 4 factorial scheme, consisting of five different percentages of biochar (with 0, 25, 50, 75, and 100% v/v substitution of the conventional substrate) and four particle sizes (<1, 2, 4, and 9 mm), with nine repetitions. As seedling growth variables, the average sprouting time, sprouting speed index, plant height, leaf number, leaf length, and width + 2, as well as the dry mass of the aerial parts and roots were evaluated. Irrespective of the percentage of commercial substrate replaced with biochar, sprouting time was found to be shorter when 6-mm-diameter biochar particles were used. With respect to the sprouting speed index, it was found that regardless of particle size, the highest value occurred when biochar was used to replace 42% of the commercial substrate. The substitution of the commercial substrate with biochar had the effect of reducing the growth of sugarcane seedlings.

scholarly journals Respon Pertumbuhan Tanaman Gelombang Cinta Akibat Pemberian Pupuk Daun Petrovita di Pembibitan Lanjutan

2013 ◽  
Vol 13 (1) ◽  
pp. 10-18
Author(s):  
Djoko Eko Hadi Susilo ◽  
Sri Handayani Wahyuningsih
Keyword(s):  
Love Wave ◽  
Growth Response ◽  
Leaf Length ◽  
Stem Height ◽  
Efficient Treatment ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Leaf Fertilizer ◽  
Treatment Design

This study aims to determine the growth response of the love wave plant (Anthurium plowmanii) on Petrovita leaf fertilizer in an advanced nursery conducted in August - October 2011 in Panarung Village, Pahandut District, Palangka Raya City. Single treatment design (one factor) with 10 (ten) repetitions in the form of Petrovita leaf fertilizer (D) consisting of 5 concentration levels, namely: d1 = 0.5 ml l-1 (0.05%); d2 = 1.0 ml l-1 (0.10%); d3 = 1.5 ml l-1 (0.15%); d4 = 2.0 ml l-1 (0.20%); and d5 = 2.5 ml l-1 (0.25%) using a completely randomized design. Observations were made when the plants in the advanced nurseries had received treatment applications 4 times at intervals of 2 weeks. Observations were made on plant height (cm); stem height (cm); number of leaves (leaves); leaf length (cm); leaf width (cm); and leaf area (cm2). The results of this study indicate that Petrovita complete leaf fertilizer has a very significant effect on increasing the growth of love wave plants aged 8 MST in advanced nurseries. Petrovita fertilizer concentration of 0.2% or 2 ml l-1 (d4) is the best and most efficient treatment for love wave plants in advanced nurseries. It is recommended to increase the growth of love wave plants in advanced nurseries using Petrovita 0.2% concentration but tried with an interval of less than 2 weeks (for example once a week).

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