scholarly journals Typology of Breeding Sites and Species Diversity of Culicids (Diptera: Culicidae) in Akanda and its Environs (North West, Gabon)

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Silas Lendzele Sevidzem ◽  
Richard Pamba ◽  
Aubin A. Koumba ◽  
Christophe R. Zinga-Koumba ◽  
Alexis Mbouloungou ◽  
...  
Keyword(s):  
Species Diversity ◽  
Anopheles Gambiae ◽  
Control Strategies ◽  
World Health ◽  
Mosquito Larvae ◽  
Breeding Sites ◽  
Larval Habitats ◽  
North West ◽  
Anopheles Gambiae S.L ◽  
Health Organization

The typology of larval habitats and the species diversity of culicids were conducted in the Akanda and its environs by collecting mosquito larvae and nymphs present in water collections, from May to June 2019. A total of 334 breeding sites of which 158 positive (47.31%) and 176 negative (52.69%) were identified. Water storage media such as: barrels (n = 95; 28.44%), puddles (n = 73; 21.86%), ponds (n = 47; 14.07%), tires (n = 31; 9.28%) and drains (n = 22; 6.59%) were the most frequent types of breeding sites. In addition, 1 549 mosquito larvae were collected, of which 59.39% belong to the sub-family Culicinae and 40.61% belong to the sub family Anophelinae. After rearing, 790 adults were obtained and divided into 8 species, including Aedes albopictus, Aedes aegypti, Anopheles gambiae s.l., Anopheles pharaoensis, Culex tigripes, Culex decens, Culex quinquefasciatus, and Mansonia uniformis. Although preliminary, this information on the culicid fauna could be useful for the implementation of appropriate control strategies in the study area. Likewise, the presence of Anopheles gambiae s.l. in this area requires that additional studies on its resistance to insecticides be conducted as recommended by the World Health Organization.

scholarly journals Pyrethroid and Etofenprox Resistance in Anopheles gambiae and Anopheles coluzzii from Vegetable Farms in Yaoundé, Cameroon: Dynamics, Intensity and Molecular Basis

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5543
Author(s):  
Michael Piameu ◽  
Philippe Nwane ◽  
Wilson Toussile ◽  
Konstantinos Mavridis ◽  
Nadja Christina Wipf ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Mortality Rates ◽  
World Health ◽  
Anopheles Coluzzii ◽  
Cross Sectional ◽  
Metabolic Genes ◽  
Anopheles Gambiae S.L ◽  
Underlying Mechanisms ◽  
Health Organization ◽  
The City

Previous studies have indicated widespread insecticide resistance in malaria vector populations from Cameroon. However, the intensity of this resistance and underlying mechanisms are poorly known. Therefore, we conducted three cross-sectional resistance surveys between April 2018 and October 2019, using the revised World Health Organization protocol, which includes resistance incidences and intensity assessments. Field-collected Anopheles gambiae s.l. populations from Nkolondom, Nkolbisson and Ekié vegetable farms in the city of Yaoundé were tested with deltamethrin, permethrin, alpha-cypermethrin and etofenprox, using 1× insecticide diagnostic concentrations for resistance incidence, then 5× and 10× concentrations for resistance intensity. Subsamples were analyzed for species identification and the detection of resistance-associated molecular markers using TaqMan® qPCR assays. In Nkolbisson, both An. coluzzii (96%) and An. gambiae s.s. (4%) were found together, whereas only An. gambiae s.s. was present in Nkolondom, and only An. coluzzii was present in Ekié. All three populations were resistant to the four insecticides (<75% mortality rates―MR1×), with intensity generally fluctuating over the time between mod-erate (<98%―MR5×; ≥98%―MR10×) and high (76–97%―MR10×). The kdr L995F, L995S, and N1570Y, and the Ace-1 G280S-resistant alleles were found in An. gambiae from Nkolondom, at 73%, 1%, 16% and 13% frequencies, respectively, whereas only the kdr L995F was found in An. gambiae s.s. from Nkolbisson at a 50% frequency. In An. coluzzii from Nkolbisson and Ekié, we detected only the kdr L995F allele at 65% and 60% frequencies, respectively. Furthermore, expression levels of Cyp6m2, Cyp9k1, and Gste2 metabolic genes were highly upregulated (over fivefold) in Nkolondom and Nkolbisson. Pyrethroid and etofenprox-based vector control interventions may be jeopardized in the prospected areas, due to high resistance intensity, with multiple mechanisms in An. gambiae s.s. and An. coluzzii.

scholarly journals ImergardTMWP: A Non-Chemical Alternative for an Indoor Residual Spray, Effective against Pyrethroid-Resistant Anopheles gambiae (s.l.) in Africa

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 322 ◽  
Author(s):  
Jean M. Deguenon ◽  
Roseric Azondekon ◽  
Fiacre R. Agossa ◽  
Gil G. Padonou ◽  
Rodrigue Anagonou ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Mosquito Control ◽  
Residual Activity ◽  
Sub Saharan Africa ◽  
World Health ◽  
Wild Type ◽  
Anopheles Gambiae S.L ◽  
Sub Saharan ◽  
Health Organization ◽  
Assay Conditions

Malaria is the deadliest mosquito-borne disease and kills predominantly people in sub-Saharan Africa (SSA). The now widespread mosquito resistance to pyrethroids, with rapidly growing resistance to other insecticide classes recommended by the World Health Organization (WHO), may overturn the successes gained in mosquito control in recent years. It is of utmost importance to search for new, inexpensive, and safe alternatives, with new modes of action, that might improve the efficacy of current insecticides. The efficacy of a novel mechanical insecticidal mineral derived from volcanic rock, ImergardTMWP, was investigated to determine its efficacy as a stand-alone residual wall spray and as a mixture with deltamethrin (K-Othrine® Polyzone) in experimental huts in Cove, Benin. The evaluation was conducted with susceptible (Kisumu) and wild-type Anopheles gambiae (s.l.). Deltamethrin applied alone demonstrated 40–45% mortality (at 72 h post-exposure) during the first four months, which declined to 25% at six months for wild An. gambiae from Cove. ImergardTMWP alone and mixed with deltamethrin, under the same assay conditions, produced 79–82% and 73–81% mortality, respectively, during the same six-month period. ImergardTMWP met the 80% WHO bio-efficacy threshold for residual activity for the first five months with 78% residual activity at six months. ImergardTMWP can be used as a mixture with chemical insecticides or as a stand-alone pesticide for mosquito control in Africa.

scholarly journals Personal protection with PBO-pyrethroid synergist-treated nets after 2 years of household use against pyrethroid-resistant Anopheles in Tanzania

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jackline L. Martin ◽  
Franklin W. Mosha ◽  
Eliud Lukole ◽  
Mark Rowland ◽  
Jim Todd ◽  
...  
Keyword(s):  
Pyrethroid Resistance ◽  
Density Ratio ◽  
Household Survey ◽  
Sensu Stricto ◽  
World Health ◽  
Malaria Vectors ◽  
Personal Protection ◽  
North West ◽  
Hole Index ◽  
Health Organization

Abstract Background The spread of pyrethroid resistance in malaria vectors threatens the effectiveness of standard long-lasting insecticidal nets (LLIN). Synergist nets combine pyrethroid (Py) and piperonyl-butoxide (PBO) to enhance potency against resistance mediated by mono-oxygenase mechanisms. Our project assessed personal protection of the World Health Organization first-in-class PBO-Py LLIN (Olyset Plus) versus the standard LLIN (Olyset net) against pyrethroid-resistant Anopheles gambiae sensu lato (s.l.) and An. funestus in North-West Tanzania after 20 months of household use. Methods From a household survey, 39 standard Olyset net and 39 Olyset Plus houses were selected. The physical integrity and hole index (HI) of the nets were assessed, and resting mosquitoes were collected from inside nets and from room walls. The indoor abundance was estimated using CDC light traps and species identified using PCR. The bioefficacy of PBO and standard LLINs against wild Anopheles was assessed using 30-minute cylinder bioassays. Results Of 2397 Anopheles collected, 8.9% (n = 213) were resting inside standard Olyset nets, while none were found inside Olyset Plus nets (PBO-Py LLINs) of any HI category. Resting density of blood-fed mosquitoes was higher on walls of sleeping rooms with Olyset nets compared to Olyset Plus (0.62 vs 0.10, density ratio [DR]: 0.03, 95% CI 0.01–0.13, p < 0.001). Mosquitoes were found inside Olyset nets of all WHO HI categories, but more were collected inside the more damaged nets (HI ≥ 643) than in less damaged (HI 0–64) nets (DR: 6.4, 95% CI 1.1–36.0, p = 0.037). In bioassay, mortality of An. gambiae s.l. was higher with Olyset Plus than with Olyset nets for new nets (76.8% vs 27.5%) and nets used for 20 months (56.8% vs 12.8%); similar trends were observed with An. funestus. Conclusion The PBO-Py LLINs provided improved protection after 20 months of household use, as demonstrated by the higher bioassay mortality and absence of pyrethroid-resistant An. gambiae sensu stricto (s.s.) and An. funestus collected from inside Olyset Plus nets, irrespective of HI category, as compared to Olyset nets.

scholarly journals No Evidence of SARS-CoV-2 Circulation in Rome (Italy) during the Pre-Pandemic Period: Results of a Retrospective Surveillance

2020 ◽  
Vol 17 (22) ◽  
pp. 8461
Author(s):  
Carlo Capalbo ◽  
Enrico Bertamino ◽  
Alessandro Zerbetto ◽  
Iolanda Santino ◽  
Andrea Petrucca ◽  
...  
Keyword(s):  
Control Strategies ◽  
Local Level ◽  
Central Italy ◽  
Total Sample ◽  
World Health ◽  
Infection Prevalence ◽  
Health Authorities ◽  
Causative Agents ◽  
Definition Of ◽  
Health Organization

In March 2020, the World Health Organization (WHO) declared that the COVID-19 outbreak recorded over the previous months could be characterized as a pandemic. The first known Italian SARS-CoV-2 positive case was reported on 21 February. In some countries, cases of suspected “COVID-19-like pneumonia” had been reported earlier than those officially accepted by health authorities. This has led many investigators to check preserved biological or environmental samples to see whether the virus was detectable on dates prior to those officially stated. With regard to Italy, the results of a microbiological screening in sewage samples collected between the end of February and the beginning of April 2020 from wastewaters in Milan (Northern Italy) and Rome (Central Italy) showed presence of SARS-CoV-2. In the present study, we evaluated, by means of a standardized diagnostic method, the SARS-CoV-2 infection prevalence amongst patients affected by severe acute respiratory syndrome (SARI) in an academic hospital located in Central Italy during the period of 1 November 2019–1 March 2020. Overall, the number of emergency room (ER) visits during the investigated period was 13,843. Of these, 1208 had an influenza-like syndrome, but only 166 matched the definition of SARI as stated in the study protocol. A total of 52 SARI cases were laboratory confirmed as influenza: 26 as a type B virus, 25 as a type A, and 1 as both viruses. Although about 17% of the total sample had laboratory or radiological data compatible with COVID-19, all the nasopharyngeal swabs stored underwent SARS-CoV-2 RT-PCR and tested negative. Based on our result, it is confirmed that the COVID-19 pandemic spread did not start prior to the “official” onset in central Italy. Routine monitoring of SARI causative agents at the local level is critical for reporting epidemiologic and etiologic trends that may differ from one country to another and also among different influenza seasons. This has a practical impact on prevention and control strategies.

scholarly journals Modelling and Simulating the Novel Coronavirus with Implications of Asymptomatic Carriers

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Ghassane Benrhmach ◽  
Khalil Namir ◽  
Jamal Bouyaghroumni
Keyword(s):  
Control Strategies ◽  
World Health ◽  
Contact Tracing ◽  
Effective Control ◽  
Public Authorities ◽  
Asymptomatic Carriers ◽  
Social Distancing ◽  
Infected People ◽  
Novel Coronavirus ◽  
Health Organization

The World Health Organization declared that the total number of confirmed cases tested positive for SARS‐CoV‐2, affecting 210 countries, exceeded 3 million on 29 April 2020, with more than 207,973 deaths. In order to end the global COVID‐19 pandemic, public authorities have put in place multiple strategies like testing, contact tracing, and social distancing. Predictive mathematical models for epidemics are fundamental to understand the development of the epidemic and to plan effective control strategies. Some hosts may carry SARS‐CoV‐2 and transmit it to others, yet display no symptoms themselves. We propose applying a model (SELIAHRD) taking in consideration the number of asymptomatic infected people. The SELIAHRD model consists of eight stages: Susceptible, Exposed, Latent, Symptomatic Infected, Asymptomatic Infected, Hospitalized, Recovered, and Dead. The asymptomatic carriers contribute to the spread of disease, but go largely undetected and can therefore undermine efforts to control transmission. The simulation of possible scenarios of the implementation of social distancing shows that if we rigorously follow the social distancing rule then the healthcare system will not be overloaded.

scholarly journals Species diversity and breeding site of mosquito larvae (Diptera: Culicidae) in Macaca fascicularis breeding area

2021 ◽  
Vol 948 (1) ◽  
pp. 012039
Author(s):  
D Novianto ◽  
U K Hadi ◽  
S Soviana ◽  
Supriyono ◽  
H S Darusman
Keyword(s):  
Species Diversity ◽  
Macaca Fascicularis ◽  
Mosquito Species ◽  
Breeding Site ◽  
Mosquito Larvae ◽  
Breeding Sites ◽  
Vector Borne Diseases ◽  
Armigeres Subalbatus ◽  
Vector Borne

Abstract Mosquito larvae play an essential role in the ecological, and many of them can spread human and animal diseases, including in Macaca fascicularis. Information on mosquito species and their habitats can provide an overview of the role of mosquitoes in the spread of vector-borne diseases in M. fascicularis captivity area. This study aimed to identify species diversity of mosquito larvae, species affinity and association, and the larval breeding sites around M. fascicularis captivity area in Bogor, West Java. Mosquito larvae were collected from 102 sites using a 350 ml dipper. Mosquito larvae that were successfully collected consisted of 11 species; Aedes albopictus, Ae. aegypti, Armigeres subalbatus, Anopheles aconitus, An. kochi, An. vagus, Culex fuscocephala, Cx. pseudovishnui, Cx. tritaeniorhyncus, Cx. quinquefasciatus, and Cx. vishnui. Co-occurrence in mosquito larvae as many as 13 compositions, with the highest co-occurrence in Ae. albopictus and Cx. quinquefasciatus that was 11 times. There were seven types breeding sites for the larval mosquitoes, i.e containers, ditches, creeks, ponds, artificial ponds, groundwater puddles, and rice fields. We conclude the existence of mosquito larvae and the availability of their breeding site in M. fascicularis captivity area can be a potential transmission of pathogens between mosquitoes and hosts

scholarly journals Fasciolosis

2011 ◽  
Author(s):  
Michael Parkinson ◽  
John P. Dalton ◽  
Sandra M. O’Neill
Keyword(s):  
At Risk ◽  
Large Scale ◽  
Fasciola Hepatica ◽  
Control Strategies ◽  
Epidemiological Studies ◽  
World Health ◽  
Complex Life Cycle ◽  
Human Populations ◽  
Specific Stage ◽  
Health Organization

Liver fluke disease, or fasciolosis, of livestock and humans is caused by endoparasitic trematodes of the genus Fasciola. Fasciola hepatica is responsible for the disease in temperate climates whereas F. gigantica is found in tropical zones. Recently, hybrids between F. hepatica and F. gigantica have been described (Le et al. 2008, Periago et al. 2008). Fasciolosis is a true zoonoses as it is predominantly a disease of animals that can be transmitted to humans at a specific stage of the parasite’s complex life cycle. There are a number of definitive hosts which includes sheep, cattle, and humans but this parasite has evolved to infect many other mammalian hosts including pigs, dogs, alpacas, llamas, rats, and goats (Apt et al. 1993; Chen and Mott 1990; Esteban et al. 1998). While prevalence of infection in humans may be relatively low in relation to animals, in specific geographic locations, for example in Bolivia, the prevalence of fasciolosis is so high in the human populations (hyperendemic) that it contributes to the spread of disease in animals (Esteban et al. 1999; Mas-Coma et al. 1999).Archeological studies showing Fasciola eggs in ancient mummies in Egypt demonstrate that fasciolosis is an ancient human disease (David 1997). Sporadic cases of fasciolosis were reported in Egypt in 1958 (Kuntz et al. 1958). The first to carry out an extensive review on human fasciolosis were Chen and Mott (1990). They reported 2,595 cases in over 40 countries in Europe, the Americas, Asia, Africa and the western Pacifi c from 1970 – 1990. This review raised awareness of fasciolosis in humans and triggered a growth in epidemiological studies and a consequential dramatic increase in reporting of cases in the literature. Now human fasciolosis is recognized by the World Health Organization (WHO) as an important disease in humans with an estimated 2.4 million people infected annually and 180 million at risk to infection in over 61 countries (Haseeb et al. 2002). There have been several cases of large scale epidemics in France (Dauchy et al. 2007), Egypt (Curtale et al. 2007) and Iran (Rokni et al. 2002).However, the only extensive epidemiological studies to determine the rate of infection have been carried out in Egypt and Bolivia (Curtale et al. 2003, 2007; Esteban et al. 2002; Parkinson et al. 2007). These studies have shown that co-infection with other diseases is a common occurrence and this may lead to under-reporting of the incidence of fasciolosis (Esteban et al. 2003; Maiga et al. 1991). In many countries, the overall rates of infection are extrapolated from sporadic reports of the disease and, consequently, worldwide disease prevalence is uncertain. In this chapter we will review the cause and effect of human fasciolosis, and particularly highlight important considerations in designing control strategies to reduce infection in at-risk communities.

scholarly journals Composition of Mosquito Fauna and Insecticide Resistance Status of Anopheles gambiae complex in Itang special woreda, Gambella, Southwestern Ethiopia

2021 ◽  
Author(s):  
Tebiban Chanyalew ◽  
Delenasaw Yewhalaw ◽  
Eba Alemayehu Simma
Keyword(s):  
Insecticide Resistance ◽  
Anopheles Gambiae ◽  
Light Trap ◽  
Test Procedure ◽  
World Health ◽  
Site Mutation ◽  
African Countries ◽  
Breeding Sites ◽  
Phenotypic Resistance ◽  
Pirimiphos Methyl

Abstract IntroductionMalaria is a leading cause of morbidity and mortality mainly in sub-Saharan African countries. Plasmodium falciparum and P. vivax are the dominant malaria parasites responsible for the majority of malaria cases in Africa. The aim of this study was to investigate composition of mosquito fauna and insecticide resistance status of Anopheles mosquito in Itang special woreda (district), Gambella, southwestern Ethiopia.Materials and methodsAdult mosquitoes were sampled from September 2020 to Feburary 2021 using Centers for Disease Control and Prevention (CDC) light trap and Pyrethrum Spray Catch (PSC). Moreover, mosquito larvae were collected from different breeding sites and reared to adults. Susceptibility tests were conducted on adult two to three days old non blood fed female Anopheles gambiae s.l following world health organization (WHO) standard susceptibility test procedure. Insecticide impregnated papers with deltamethrin (0.05%), alpha-cypermethrin (0.05%), propoxur (0.1%), pirimiphos-methyl (0.25%) and bendiocarb (0.1%) were used to assess susceptibility status of Anopheles gambiae s.l populations in the study area. Moreover, molecular diagnostics were done for the identification of member species of Anopheles gambiae s.l and detection of knockdown resistance (kdr) using species specific polymerase chain reaction (PCR) and allele specific PCR.ResultsIn total, 468 adult mosquitoes were collected from different houses. Culex mosquitoes were the most dominant (80.4%) followed by Anopheles mosquitoes. Three species of Anopheles mosquitoes (An. coustani, An. pharoensis, and An. gambiae (s.l.)) were identified, of which An. coustani was the dominant (8.1%) species. Out of 468 adult mosquitoes, 294 were blood fed while 46 were half-gravid and gravid. The WHO bioassay tests revealed that the populations of An. gambiae s.l in the study area are resistant against alpha-cypermethrin and deltamethrin whereas, susceptible to bendiocarb, pirimiphos-methyl and propoxur. Out of the total 86 An. gambiae s.l specimens assayed, 79 (92%) successfully amplified and all were identified as An. arabiensis. West African Kdr (L1014F) mutation was detected with high Kdr allele frequency ranging from 67–88%.ConclusionThe detection of target site mutation, kdr L1014F allele, coupled with the phenotypic resistance against alpha-cypermethrin and deltamethrin call for continuous resistance monitoring.

scholarly journals Controlling the Spread of COVID-19: Optimal Control Analysis

2020 ◽  
Author(s):  
Chinwendu E. Madubueze ◽  
Sambo Dachollom ◽  
Isaac Obiajulu Onwubuya
Keyword(s):  
Optimal Control ◽  
Control Strategies ◽  
Public Health Education ◽  
Time Dependent ◽  
World Health ◽  
Critical Parameters ◽  
Control Analysis ◽  
Invariant Region ◽  
Control Approach ◽  
Health Organization

AbstractCoronavirus disease 2019 (COVID-19) is a disease caused by Severe acute respiratory syndrome coronavirus 2 (SARS CoV-2). It was declared on March 11, 2020, by the World Health Organization as pandemic disease. The disease has neither approved medicine nor vaccine and has made government and scholars search for drastic measures in combating the pandemic. Regrettably, the spread of the virus and mortality due to COVID-19 has continued to increase daily. Hence, it is imperative to control the spread of the disease particularly using non-pharmacological strategies such as quarantine, isolation and public health education. This work studied the effect of these different control strategies as time-dependent interventions using mathematical modeling and optimal control approach to ascertain their contributions in the dynamic transmission of COVID-19. The model was proven to have an invariant region and was well-posed. The basic reproduction number was computed with and without interventions and was used to carry out the sensitivity analysis that identified the critical parameters contributing to the spread of COVID-19. The optimal control analysis was carried out using the Pontryagin’s maximum principle to figure out the optimal strategy necessary to curtail the disease. The findings of the optimal control analysis and numerical simulations revealed that time-dependent interventions reduced the number of exposed and infected individuals compared to time-independent interventions. These interventions were time-bound and best implemented within the first 100 days of the outbreak. Again, the combined implementation of only two of these interventions produced a good result in reducing infection in the population, while the combined implementation of all three interventions performed better, even though zero infection was not achieved in the population. This implied that multiple interventions need to be deployed early in order to the virus to the barest minimum.

scholarly journals Choice of unmanned aerial vehicles for identification of mosquito breeding sites

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Franciely Velozo Aragão ◽  
Fernanda Cavicchioli Zola ◽  
Luis Henrique Nogueira Marinho ◽  
Daiane Maria De Genaro Chiroli ◽  
Aldo Braghini Junior ◽  
...  
Keyword(s):  
Unmanned Aerial Vehicles ◽  
Urban Areas ◽  
High Mobility ◽  
World Health ◽  
Breeding Sites ◽  
Manual Search ◽  
Aerial Vehicles ◽  
The World ◽  
Mosquito Breeding ◽  
Health Organization

The disordered urban growth that may favour the emergence of the Aedes aegypti mosquito in cities is a problem of increasing magnitude in middle- and high-income countries in the tropical part of the world. Currently, the World Health Organization (WHO) considers the control and elimination of Ae. aegypti a world-wide high priority as it is the main vector of many rapidly spreading viral diseases, dengue in particular. A major difficulty in controlling the proliferation of this vector is associated with identification of the breeding sites. The use of Unmanned Aerial Vehicles (UAVs) can be an efficient alternative to manual search because of high mobility and the ability to overcome physical obstacles, particularly in urban areas where it can offer close-up images of potential breeding sites that are difficult to reach. The objective of this study was to find a way to select the most suitable UAV for the identification of Ae. aegypti habitats by providing images of potential mosquito breeding sites. This can be accomplished by a Multiple-Criteria Decision Method (MCDM) based on an Analytical Hierarchy Process (AHP) for the evaluation of weights of the criteria used for characterizing UAVs. The alternatives were analyzed and ranked using the Fuzzy Set Theory (FST) merged with the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS). The methodology is explained and discussed with respect to identification and selection of the most appropriate UAV for aerial mapping of Aedes breeding sites.

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