scholarly journals Optimization of Extraction Parameters of Polyphenols from Mango Seed Kernel through Response Surface Methodology

2020 ◽  
Vol 5 (8) ◽  
pp. 877-883
Author(s):  
Junior Franck Ekorong Akouan Anta ◽  
Dorothée Marcelle Biloa ◽  
K.S.M.S. Raghavarao
Keyword(s):  
Phenolic Compounds ◽  
Reducing Power ◽  
Extraction Yield ◽  
Agitation Speed ◽  
Extraction Time ◽  
Seed Kernel ◽  
Extraction Temperature ◽  
Mango Seed Kernel ◽  
Extraction Parameters ◽  
Mango Seed

Phenolic compounds constitute an essential part of the human diet, and are of considerable interest due to their antioxidant properties. The traditional maceration method has been used for the extraction of polyphenols from mango seed kernel (Mangifera indica L.). Highlight the effects of different extraction parameters is useful to optimize the process, as well as to predict the extraction yield within the experimental domain with enough precision and confidence. The purpose of this work is to probe the influence of extraction time, extraction temperature and agitation speed on the extraction yield of phenolic compounds, and the total reducing power of the extract. The Surface Methodology (RSM), using the Doehlert design, have been applied. Optimal values of extraction yield and total reducing power was 36.99 mg Gallic acid equivalent/g and 61.08 mg Ascorbic Acid Equivalent/g respectively. The optimal conditions are 60 minutes of extraction time, 68.7°C extraction temperature and 424 rpm for agitation speed. Under optimized conditions the experimental values well agreed with the values predicted by the model equations proposed.

Optimization of Extraction of Bioactive Material-Polysaccharides from the Roots of Taraxacum mongolicum by Box-Behnken Experimental Design

2013 ◽  
Vol 749 ◽  
pp. 354-358
Author(s):  
Kai Chang ◽  
Guang Ren Sun ◽  
Da Di Yao
Keyword(s):  
Experimental Design ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Time ◽  
Changbai Mountains ◽  
Extraction Temperature ◽  
Single Variable ◽  
Medical Systems ◽  
Extraction Parameters ◽  
Materials Used

Dandelion (Taraxacum spp) is an medical active materials used in many traditional and modern herbal medical systems, its polysaccharides have many bioactivities. Taraxacum mongolicum is one of the most widely consumed as Chinese herbal medicine and root tea in Changbai Mountains area, Northeast China. In this study, a procedure for the extraction of polysaccharides from the root of T mongolicum(TMRPS) was established. Based on single-variable tests experiment to determine the ranges of the extraction parameters (extraction temperature (X1), extraction time (X2), and ratio of solvent to raw material (X3)), response surface methodology (RSM) was used to optimize for these extraction parameters (X1, X2, X3) enhancing the forward extraction efficiency of TMRPS by a three-level three-variable Box-Behnken experimental design. The results showed that the ranges of the extraction parameters were determined as 60°C-80°C(X1),1h-3h (X2),20-40(v/m)(X3) by single-variable tests and the maximal yield (9.01%) of TMRPS was reached at extraction temperature 70.2°C, extraction time 1.79 h and ratio of solvent to raw material 28.0 (v):1 (m). Convenient for experiment, appropriate readjustment of these extraction parameters was made. Under these readjusted conditions, the mean extraction yield of TMRPS was 8.85%, which was near to theoretical calculation and indicates that these results were agree closely with model predictions.

scholarly journals Optimizing the Extraction of Phenolic Compounds with High Anti-oxidant Activity from Mango Seed Kernel Wastes Using Response Surface Methodology

2020 ◽  
pp. 60-76
Author(s):  
Francis Dave C. Siacor ◽  
Camila Flor Y. Lobarbio ◽  
Evelyn B. Taboada
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Seed Kernel ◽  
Extraction Temperature ◽  
Positive Effects ◽  
Water Solvent ◽  
Anti Oxidant ◽  
Mango Seed

Mango seed kernels (MSK), which are waste streams in mango processing industry, are good sources of phenolic compounds with high anti-oxidant, anti-bacterial, and anti-viral properties. These phenolic compounds are reported to have an increasing demand over the years in the quest for healthy ready-to-eat food and nutraceuticals. To recover these compounds from MSK, solid-liquid extraction (SLE) can simply be applied, although there is a knowledge gap in in the systematic exploration of this process for mango-based phenolic compounds. In this work, phenolic compounds were extracted from MSK through SLE using ethanol-water solvent system. A statistical-based approach was used to evaluate and optimize the extraction conditions in relation to yield of phenolic compounds from MSK. The central composite design together with response surface methodology was adopted to assess the effect of extraction temperature (30oC, 45oC, and 60oC) and ethanol concentration (25% – 75%) under fixed extraction time (105 min) and solid-to-solvent ratio (1:10) on the extraction yield. Both temperature and ethanol concentration provided positive effects on the yield and the following optimum conditions of temperature and ethanol concentration were obtained at 63.21oC and 53.21% ethanol, respectively. A second-order polynomial equation was obtained describing the extraction process, and a validation test of this response model showed that it sufficiently described the process. Furthermore, the extracts obtained at optimal conditions possess a potential antioxidant activity at IC50 = 45 ± 0.002 µg GAE mL-1 extract. The results indicated that phenolic compounds in MSK can be recovered through extraction using aqueous ethanol. This study also promotes value-addition of a seemingly useless waste material while reducing its health and environmental impact.

scholarly journals Optimization of Alkaline Extraction and Bioactivities of Polysaccharides from Rhizome ofPolygonatum odoratum

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Yong Chen ◽  
Luoyi Yin ◽  
Xuejiao Zhang ◽  
Yan Wang ◽  
Qiuzhi Chen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Dpph Radical ◽  
Key Factor ◽  
Extraction Parameters ◽  
Dpph Radical Scavenging

The present study is to explore the optimal extraction parameters, antioxidant activity, and antimicrobial activity of alkaline soluble polysaccharides from rhizome ofPolygonatum odoratum. The optimal extraction parameters were determined as the following: NaOH concentration (A) 0.3 M, temperature (B)80°C, ratio of NaOH to solid (C) 10-fold, and extraction time (D) 4 h, in which ratio of NaOH to solid was a key factor. The order of the factors was ratio of NaOH to solid (fold, C) > extraction temperature (°C, B) > NaOH concentration (M, A) > extraction time (h, D). The monosaccharide compositions of polysaccharides fromP. odoratumwere rhamnose, mannose, xylose, and arabinose with the molecular ratio of 31.78, 31.89, 11.11, and 1.00, respectively. The reducing power, the 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging rate, the hydroxyl radicals scavenging rate, and the inhibition rate to polyunsaturated fatty acid (PUFA) peroxidation of the alkaline soluble polysaccharides fromP. odoratumat 1 mg/mL were 9.81%, 52.84%, 19.22%, and 19.42% of ascorbic acid at the same concentration, respectively. They also showed antimicrobial activity against pathogenic bacteriaStaphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, andEscherichia coli.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Partial Purification of Polyphenol Oxidase (PPO) from Mango Seed Kernel (MSK) using Alcohol/Salt-based Aqueous Two-Phase System (ATPS)

2021 ◽  
Vol 10 ◽  
Author(s):  
John Leanard M. Lasquite ◽  
Brendaline C. Enopia ◽  
Roger Francis E. Senangote ◽  
Danilo C. Sabarre Jr. ◽  
Camila Flor Yagonia-Lobarbio
Keyword(s):  
Polyphenol Oxidase ◽  
Alternative Methods ◽  
Phase System ◽  
Seed Kernel ◽  
Mass Ratio ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Activity Recovery ◽  
Mango Seed Kernel ◽  
Mango Seed

Background: Polyphenol oxidase (PPO) has applications in pharmaceutical, environmental, and food industries, however, conventional techniques for its purification are considered costly and laborious. Alternative methods of purification are needed. Methods: This study explored the use of an alcohol/salt-based aqueous two-phase system (ATPS) to partially purify PPO from mango seed kernel (MSK). Specifically, this study determined the effects of 1-propanol/potassium phosphate buffer mass ratio on the partition parameters such as partition coefficients, activity recovery, and purification fold. Results: The enzyme partition coefficient (K E ) was greater than 1, which signified that more PPO was partitioned in the top phase due to its hydrophobic nature. Specifically, the 3.22 1-propanol/PPB mass ratio had the highest K E of 1.91 ± 0.16 and 1.46 ± 0.16 for runs 1 and 2, respectively. Moreover, the same mass ratio led to the highest activity recovery in the top phase reaching up to 73.99 ± 1.56% for run 1 and 64.27 ± 2.48% for run 2. In terms of purification fold, runs 1 and 2 had PF values of 0.55 ± 0.05 and 0.70 ± 0.01, respectively. Since the values obtained were low due to the large number of contaminants extracted together with the target enzyme in the top phase, the use of a back-extraction step recovered the PPO in the bottom phase and resulted in an activity recovery of 82.94 ± 1.05% and a purification fold of 7.01 ± 0.95. Conclusion: Results have shown that varying the mass ratio had significant effects on the partition parameters.

scholarly journals Optimization of Supercritical Carbon Dioxide Extraction of Quercus infectoria Oil

2015 ◽  
Vol 74 (7) ◽  
Author(s):  
Liza Md Salleh ◽  
Stashia ELeaness Rosland Abel ◽  
Gholamreza Zahedi ◽  
Russly Abd Rahman ◽  
Hasmida Mohd Nasir ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Goodness Of Fit ◽  
Maximum Yield ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Predicted Values ◽  
Artificial Neural Network Ann ◽  
Quercus Infectoria

This current study focuses on the modelling and optimization of supercritical fluid extraction of Quercus infectoria galls oil. In this case, response surface methodology (RSM) and artificial neural network (ANN) were applied for the modelling and prediction of extraction yield of galls oil. A 17-run Box-Behnken Design (BBD) was employed to statistically optimize the process parameters of SC-CO2 extraction of Quercus infectoria galls at a condition as follows: pressure (5000, 6000, 7000 Psi), temperature (40, 50, 60°C) and extraction time (30, 45, 60 min). The maximum yield of the extracted oil is1.12 % and the optimum conditions are at an extraction pressure of 5574 Psi; extraction temperature of 75°C and extraction time of 54 min. Under the optimal conditions, the experimental results agree with the predicted values obtained through analysis of variance (ANOVA). This indicates a successful response surface methodology and highly satisfactory goodness of fit of the model used. The analysis of experimental design for process optimization results demonstrates that temperature and extraction time are the main parameters that influence the oil extraction of Quercus infectoria.

scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology On Phenolic, Flavonoid and Antioxidants Properties

2018 ◽  
Author(s):  
Khurul Ain Mohamed Mahzir ◽  
Siti Salwa Abdul Gani ◽  
Nor Fadzillah Mokhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Feed Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Phaleria Macrocarpa

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

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