scholarly journals Quantitative Phytochemical Composition and Bioactive Constituents of Ethanolic Extract of Phyllanthus amarus (schum. et thonn) Leaves

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
E. O. Oshomoh ◽  
O. Uzama-Avenbuan
Keyword(s):  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Total Content ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Phyllanthus Amarus ◽  
Bioactive Constituents ◽  
Phytochemical Composition ◽  
Ms Analysis ◽  
Traditional Practitioners

Herbs are used in the treatment of several human ailment and diseases. The quantitative phytochemical composition, bioactive constituents of ethanol extract of Phyllanthus amarus leaves were determined. The quantitative phytochemical analysis of ethanol extract of Phyllanthus amarus found to be present in this study includes phenol, flavonoid, saponin, tannin and alkaloid.  The total content of phenol is 73.163µg/ml, flavonoid is 137.27µg/ml, tannin is 3.581µg/ml, alkaloid is 30.1 % and saponin is 34.822 ppm. The GC-MS analysis of bioactive constituents of the ethanol extract of Phyllanthus amarus leaves resulted in 44 compounds which have diverse use.  The various bioactive compounds present in P. amarus confirm its uses for the treatment of various ailments and diseases by traditional practitioners.  

scholarly journals Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 90-94
Author(s):  
P Sasikala ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
M Padma ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fine Powder ◽  
Ethanol Extract ◽  
Taraxacum Officinale ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Biological Compounds ◽  
Phytochemical Compounds ◽  
Medicinal Properties ◽  
Phytochemical Constituents

Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds. Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis. Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial  and antidiabetic compounds. Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various  medicinal  properties to cure dangerous diseases and disorders. Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds.

scholarly journals Preliminary studies on different extracts of some honey bee products

2013 ◽  
Vol 5 (2) ◽  
pp. 420-422 ◽  
Author(s):  
Rajinder Kaur ◽  
Preeti Kalia ◽  
Neelima R. Kumar ◽  
Kusum Harjai
Keyword(s):  
Honey Bee ◽  
Bioactive Compounds ◽  
Reducing Sugars ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Bioactive Constituents ◽  
Bee Pollen ◽  
Phytochemical Composition ◽  
Ethanolic Extract Of Propolis

The aim of present study was to compare the phytochemical composition of different extracts of bee pollen, propolis and honey and to analyse the best solvent for maximum phytochemicals extraction. These preliminary studies are significant in locating the pharmacological bioactive compounds in different extracts of bee products. The tests showed greater number of constituents present in bee pollen and propolis as compared to honey. The concentration of these bioactive constituents was greater in ethanolic extract of propolis and water extract of bee pollen and honey. Ethanolic extract of propolis showed higher concentration of tannins and glycosides. Bee pollen had more concentration of saponins, flavonoids and proteins in water extract. Reducing sugars and carbohydrates were present in larger amount in water extract of honey. These studies will be useful to select the best solvent for honey, pollen and propolis for various research purposes.

scholarly journals GC-MS Analyses of Bioactive Compounds of Ethanolic Seed Extract of Elaeocarpus serratus

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
DH Geetha ◽  
Indhiramuthu Jayashree ◽  
M Rajeswari
Keyword(s):  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Seed Extract ◽  
Ms Analysis ◽  
Phytochemical Constituents ◽  
Traditional Practitioners ◽  
Novel Drug ◽  
Area Percentage

The present investigation was carried out to determine the possible bioactive compounds of ethanolic extract of Elaeocarpus serratus (Elaeocarpaceae) has been subjected to GC-MS analysis. Thirty compounds were detected from the plant E. serratus. The highest peak area percentage of 19.12% was obtained by n-propanol (RT=3.04min.) and lowest peak area percentage of 2.34% was obtained by 1-propylthio-3,3,3-trifluoropropyl acetate (RT=11.57min.). The presence of various bioactive compounds confirms many aliments by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF BIOACTIVE CONSTITUENTS IN THE ETHANOLIC EXTRACT OF EMPTY FRUIT BUNCHES

2020 ◽  
Vol 4 (2) ◽  
pp. 70-74
Author(s):  
Sameer M. Al- Al-Gorany ◽  
Saba Z. AL-Abachi ◽  
Abdulwadood I. Arif ◽  
Entesar E. Aboglida ◽  
Entesar H. Al_Abdeli
Keyword(s):  
Mass Spectra ◽  
Acid Hydrazide ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Powdered Sample ◽  
Bioactive Constituents ◽  
Empty Fruit Bunches ◽  
Ms Analysis ◽  
Phytochemical Compounds

For thousands of years plants have been an essential medicinal source with qualities. Empty fruit bunches (EFB) have medicinal values. Ten grams of powdered sample was extracted with 50 mL ethanol overnight and filtered through ash less filter paper, this plant’s ethanol extract has been analyzed using Gas Chromatography–Mass Spectrometry (GC-MS), while the compound mass spectra contained in the extract has been matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis provided different peaks determining the presence of eight different phytochemical compounds namely10-Undecenoic acid, Propanoic acid, Cyclopentane acetic acid hydrazide,1,4-Cyclohexanedicarbonitrile.The compounds were identified by comparing their retention time and peak area with literature and by interpreting the mass spectra. Many of them have antioxidant, anti-inflammatory, antimicrobial, antifungal, antitumor, antiviral, anticonvulsant, analgesic and antidiabetic properties.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals Phytochemical Identification, Acute, and Sub-Acute Oral Toxicity Studies of the Foliar Extract of Withania frutescens

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4528 ◽  
Author(s):  
Abdelfattah EL Moussaoui ◽  
Mohammed Bourhia ◽  
Fatima Zahra Jawhari ◽  
Hamza Mechchate ◽  
Meryem Slighoua ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Clinical Symptoms ◽  
Ethanolic Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Oral Toxicity ◽  
Phytochemical Composition ◽  
Subacute Toxicity ◽  
Repeated Doses

Withania frutescens (W. frutescens) is a medicinal plant widely used to treat several diseases. This work aims to study phytochemical composition as well as acute and subacute toxicity of W. frutescens hydroethanolic extract in mice. The phytochemical composition of W. frutescens extract was performed using gas chromatographic analysis. Acute toxicity was studied in vivo with oral administration of single doses 400 mg/kg, 1000 mg/kg, and 2000 mg/kg for 14 days. Subacute toxicity was studied with the administration of repeated doses of 400 mg/kg/day and 2000 mg/kg/day for 28 days. Phytochemical analysis of W. frutescens hydro-ethanolic extract confirmed the presence of interesting chemical compounds. Acute toxicity results showed no toxic symptoms in mice treated with an increasing dose up to a maximum of 2000 mg/kg. Alongside acute toxicity, subacute data showed no clinical symptoms nor biochemical or histological alteration in mice treated with an increasing dose up to a maximum of 2000 mg/kg compared to the control group (p < 0.05). This study shows no toxic effects in animals treated with W. frutescens extract, and, therefore, this plant can be considered safe in animals up to 2000 mg/kg under both acute and subacute toxicity conditions.

Preliminary phytochemical analysis and evaluation of antioxidant, cytotoxic and inhibition of lipopolysaccaride - induced NOS (iNOS) expression in BALB/c mice liver by Ziziphus oenoplia Mill. fruit

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Ramaraj Thirugnanasampandan ◽  
Gunasekar Ramya ◽  
Gunasekaran Bhuvaneswari ◽  
Srinivasan Aravindh ◽  
Suresh Vaishnavi ◽  
...  
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Tamil Nadu ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Wide Range ◽  
Mice Liver

AbstractBackground:Mill. is an ethnomedicinal plant and its fruit has been traditionally used by Puliar tribes of Anamalai Hills, Tamil Nadu, India to treat various ailments. Phytochemical analysis, antioxidant, cytotoxic and inducible nitric oxide synthase (Methods:To explore bioactive compounds present in the ripened fruits, high-performance thin-layer chromatography (HPTLC) and gas chromatography/mass spectrometry (GC–MS) analysis were done. Free radical scavenging, hepatoprotective, inhibition ofResults:Total flavonoid content of ZOFwas estimated as 69 µg/mg catechin equivalent. HPTLC densitogram confirmed the presence of quercetin and GC–MS analysis showed a total of 16 compounds of 87.66 % with quinic acid as a major compound which accounted for 22.29 %. Free radical-scavenging activity of ethanolic fruit extract was ranged from 160.12 to 650.23 µg/mL. An amount of 1.5 µg lipopolysaccharide (LPS)- induced severe inflammation in BALB/c mice liver, followed by treatment with ethanolic fruit extract of 100 µg concentration, exhibited significant hepatoprotection and reverse transcriptase polymerase (RT-PCR) analysis showed downregulation ofConclusions:Findings of the present study prove that ZOF is a rich source of bioactive compounds with a wide range of pharmacological activities. Hence, consumption of this wild edible fruit will be a cost-effective and easily available natural nutritional source for health protection.

scholarly journals PRELIMINARY PHYTOCHEMICAL STUDIES OF SELECTED MEDICINAL PLANT PACHYGONE OVATA (POER.) HOOK.F. & THOMS FROM MENISPERMACEAE FAMILY FOR BIOACTIVE CONSTITUENTS

2018 ◽  
Vol 5 (2) ◽  
pp. 50-52
Author(s):  
Renjini Haridas ◽  
Radhakrishnan G ◽  
Reshma R ◽  
Sumathi P
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Phytochemical Studies ◽  
Leaf Part ◽  
Important Medicinal Plant

The present study deals with the phytochemical examination of Pachygone ovata (Poer.) Hook.f.& Thoms., an important medicinal plant from menispermaceae family. Leaf and Stem extracts were prepared by using different solvents systems and phytochemical screening was performed using the standard methods given by Harborne. Leaf and stem extracts were prepared from aqueous and organic solvents like petroleum ether, acetone, ethyl acetate and ethanol. Qualitative phytochemical analysis of the petroleum ether, acetone,ethyl acetate, ethanol and aqueous extracts prepared from P. ovata leaf and stem part. Leaf part revealed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, phenols and tannins. Stem part revealed the presence of alkaloids, flavonoids, glycosides, Resin, Steroids, phenols and tannins. The ethanolic extract showed higher amount of secondary metabolites than the other solvent extracts. This observation becomes important in the context of the therapeutically and drug applications of P. ovata.

scholarly journals GC–MS study on ethanolic extract of Vetiveria lawsonii root

2016 ◽  
Vol 5 (3) ◽  
pp. 108-111
Author(s):  
P Ramachandran ◽  
◽  
Viji Saral Elezabeth D Elezabeth D ◽  
Keyword(s):  
Mass Spectrometer ◽  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Pharmacological Activities ◽  
Ms Analysis

The present study was focused to scrutinize the presence of phytoconstituents in the ethanolic extract of Vetiveria lawsonii root using GC-MS. The GC-MS analysis of Vetiveria lawsonii root was performed using Agilent 6890-JEOL GC-Mate-II Mass Spectrometer. The result of the study showed the presence of six bioactive compounds in the ethanolic extract. On account of this investigation, we can conclude that the ethanolic extract of Vetiveria lawsonii root shows the presence of 6 bioactive phytoconstituents. This might be the cause for the various pharmacological activities of the plant.

scholarly journals GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Waras Nurcholis ◽  
NURUL KHUMAIDA ◽  
MARIA BINTANG ◽  
MUHAMAD SYUKUR
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Anticancer Activity ◽  
Cell Line ◽  
Bioactive Compounds ◽  
Anticancer Agent ◽  
Ethanol Extract ◽  
Ms Analysis ◽  
Ethanol Extracts ◽  
Mcf 7

Abstract. Nurcholis W, Khumaida N, Bintang M, Syukur M. 2021. GC-MS analysis of rhizome ethanol extracts from Curcuma aeruginosa accessions and their efficiency activities as anticancer agent. Biodiversitas 22: 1179-1186. This work aimed to evaluate the bioactive compounds and anticancer activity in rhizome extract of ten Curcuma aeruginosa accessions to explore their pharmacological values. The GC-MS analysis was used to identify bioactive compounds. The cytotoxic activity was performed against MCF-7 (Human breastadeno carcinoma) and Vero cell lines using MTT assay. The GC-MS analysis revealed 71 of the compounds as sesquiterpenes (36), monoterpenes (20), phenolics (5), diterpenes (4), phenanthrene (1), tetrapeptides (1), oxazole (1), triazine (1), piperidine (1), and oxygenated hydrocarbons (1). The isocurcumenol was the most dominant metabolite in ethanol extract of C. aeruginosa rhizome, with the highest produced by KP accession (22.01%) followed MD accession (21.12%). However, camphor and β-elemene were the metabolites produced by all accessions studied. In the Vero cell line as a normal cell, the cytotoxic activity varied from 13.28% (MD) to 45.17% (PW). Furthermore, the cytotoxic activity ranged from 1.16% (LC) to 49.70% (MD) against the MCF-7 cell line. The highest anticancer activity was produced in MD accessions; thus, it can be used as a source of quality raw materials for the pharmaceutical and food industry. Besides that, it can also be further developed to obtain superior varieties through plant breeding programs.

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