scholarly journals PRELIMINARY PHYTOCHEMICAL STUDIES OF SELECTED MEDICINAL PLANT PACHYGONE OVATA (POER.) HOOK.F. & THOMS FROM MENISPERMACEAE FAMILY FOR BIOACTIVE CONSTITUENTS

2018 ◽  
Vol 5 (2) ◽  
pp. 50-52
Author(s):  
Renjini Haridas ◽  
Radhakrishnan G ◽  
Reshma R ◽  
Sumathi P
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Phytochemical Studies ◽  
Leaf Part ◽  
Important Medicinal Plant

The present study deals with the phytochemical examination of Pachygone ovata (Poer.) Hook.f.& Thoms., an important medicinal plant from menispermaceae family. Leaf and Stem extracts were prepared by using different solvents systems and phytochemical screening was performed using the standard methods given by Harborne. Leaf and stem extracts were prepared from aqueous and organic solvents like petroleum ether, acetone, ethyl acetate and ethanol. Qualitative phytochemical analysis of the petroleum ether, acetone,ethyl acetate, ethanol and aqueous extracts prepared from P. ovata leaf and stem part. Leaf part revealed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, phenols and tannins. Stem part revealed the presence of alkaloids, flavonoids, glycosides, Resin, Steroids, phenols and tannins. The ethanolic extract showed higher amount of secondary metabolites than the other solvent extracts. This observation becomes important in the context of the therapeutically and drug applications of P. ovata.

scholarly journals PHYTOCHEMICAL ANALYSIS OF LEAF, STEM AND ROOT IN EUPHORBIA ROTHIANA SPRENG. (EUPHORBIACEAE), THE NILGIRIS WESTERN GHATS, TAMIL NADU

2017 ◽  
Vol 4 (3) ◽  
pp. 17-18
Author(s):  
Anusuya Devi R ◽  
Veena B ◽  
Thenmozhi K
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Western Ghats ◽  
Tamil Nadu ◽  
Phytochemical Analysis ◽  
Phytochemical Constituents ◽  
Ethanol Extracts ◽  
The Nilgiris ◽  
Important Medicinal Plant

Euphorbia rothiana Spreng. is an important medicinal plant. It used in hypertensive agent in traditional medicine.The present study deals with the analysis of Phytochemical constituents by qualitative analysis of leaves, stem and root were done using Petroleum ether, chloroform, ethyl acetate and ethanol extracts. Alkaloids, flavonoids, phenols, terpenoids, triterpinoids, steroids, cardio glycosides and carbohydrates were analysed. Alkaloids, flavonoids, and phenols were highly present various extracts of leaves stem and root. Cardio glycosides triterpinoids and carbohydrates were minimum present in the various extracts.

In vitro antimicrobial activity and phytochemical analysis of fruits of Syzygium aromaticum (L.) Merr. & L.M.Perry - An important medicinal Plant

2016 ◽  
Vol 5 (4) ◽  
pp. 137-140
Author(s):  
Duraisamy Kayal Vizhi ◽  
◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam ◽  
Natarajan Nirmal Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Syzygium Aromaticum ◽  
Active Components ◽  
Methanol Extracts ◽  
Important Medicinal Plant

The fruit of Syzygium aromaticum is an important medicinal plant was subjected to phytochemical screening and antimicrobial effect of methanol, ethyl acetate and acetone extracts. Antimicrobial activity was analyzed by agar well diffusion method against gram positive and gram negative bacteria. The phytochemical analysis carried out revealed the presence of alkaloids, coumarins, catechins, flavanoids, phenols, saponins, terpenoids, tannins and steroids in various solvent extracts. Methanol extract of S. aromaticum showed good antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa and Staohylococcus aureus. It has been showed that the methanol extracts had wider range of activity on these organisms than the acetone and ethyl acetate extracts, which indicates that the methanol extracts of selected plants may contain the active components. The use of plant extracts with known antimicrobial properties can be of great significance in therapeutic treatments.

scholarly journals Antioxidant Properties of Various Parts Cissus quadrangularis L. in different solvents

2018 ◽  
Author(s):  
B. Sasi, P. Tamizhiniyan
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Antioxidant Properties ◽  
Plant Materials ◽  
Methanol Extracts ◽  
Cissus Quadrangularis ◽  
The Family ◽  
The Village ◽  
Important Medicinal Plant

An important medicinal plant Cissus quadrangularis, belonging to the family Vitaceae was used in the present study to estimate antioxidant properties of different extract (root, stem, leaves and tendrils). Plant materials were collected from the village Ponnanthittu, Chidambaram Taluk, Cuddalore Dist, The antioxidant properties of the petroleum ether, chloroform, ethyl acetate, acetone and methanol extracts of Cissus quadrangularis were screened and results showed considerable antioxidants in all the extracts.

Pharmacognostical fingerprinting and selective bioactivity studies of Solanum glaucophyllum Desf.

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Anindya Sundar Ray ◽  
Suman Kalyan Mandal ◽  
Chowdhury Habibur Rahaman
Keyword(s):  
Medicinal Plant ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Phytochemical Analysis ◽  
Antimicrobial Studies ◽  
Maximum Inhibition ◽  
Crude Drugs ◽  
Phytochemical Studies ◽  
Dpph Radical Scavenging

Solanum glaucophyllum Desf. (Solanaceae) is traditionally used for curing several health conditions in both human and domesticated animals. There is no data available regarding pharmacognostic standardization and pharmacological activity of this less known medicinal plant. Therefore, the aim of the present work is to prepare pharmacognostic fingerprints of crude drugs obtained from the leaf and stem parts of S. glaucophyllum. Apart from this detailed phytochemical analysis, antioxidant and antimicrobial studies have also been carried out. Pharmacognostic study revealed that the leaves are amphistomatic and stomata are strictly of anisocytic type. Values of stomatal index, palisade ratio, ash content of the investigated parts are found distinct and can be used as pharmacognostic standards for evaluation of crude drugs of this medicinal plant. Phytochemical studies of the leaf and stem parts indicated that phenolics, flavonoids, tannins and alkaloids are present in quite impressible amount. Curcumin content in stem was found very high through HPLC analysis. In DPPH radical scavenging assay, the stem part showed significant antioxidant potential. Ethanol extract of stem produced maximum inhibition zone (11mm) against Pseudomonas aeruginosa at the concentration of 50 mg/ml among five test microorganisms. Some pharmacognostic features recorded in this study as diagnostic ones will help in proper identification of this ethnomedicinal plant in its fresh as well as dried form. On the other hand preliminary phytochemical, antioxidant and antimicrobial studies highlight the S. glaucophyllum as a promising candidate for bioprospecting.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

scholarly journals Phytochemical analysis, anti-oxidant and anti-microbial activity of Acalypha indica leaf extracts in different organic solvents

2016 ◽  
Vol 8 (3) ◽  
pp. 444 ◽  
Author(s):  
Chekuri Sudhakar ◽  
Narendar Vankudothu ◽  
Shivaprasad Panjala ◽  
Nirmala Babu Rao ◽  
Roja Rani Anupalli
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Microbial Activity ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Soxhlet Extraction ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
Acalypha Indica

The herb <em>Acalypha indica </em>which belongs to Euphorbiaceae family has multiple medicinal properties which include anti-oxidant, anti-bacterial, anti-fungal, anti-inflammatory, antiulcer, anti-helmintic, anti-cancerous, anti-venom, and neuro-protective activity. The present study was designed to evaluate the phytochemical, antimicrobial and anti-oxidant activity of <em>Acalypha indica </em>leaves extracts in different solvent extractions like methanol, hexane, ethyl acetate and petroleum ether. Fresh leaves of the plant were collected and shade dried. Dried leaves were milled to obtain powder. Powder was subjected to soxhlet extraction using solvents and extracts were successively obtained. Phytochemical analysis was conducted following standard methods.  Phytochemical analysis showed the presence of Alkaloids, Phenols, Saponins, Flavanoids and Amino acids. Leaf extract of methonal have shown the highest anti-oxidation capacity than hexane, ethyl acetate and petroleum ether. Anti-microbial activity has been performed on microbes like <em>Bacillus sps</em>, <em>E.coli,</em> <em>Psuedomonas sps</em> and <em>Streptococcus sps.</em> A highest value of zone of inhibition was found in methanol extract against <em>E.coli.</em> These results provide evidence that <em>Acalypha indica </em>leaf extract possesses vital phytochemicals, antimicrobial and antioxidant properties. Hence this plant can be studied further for drug analysis for finding potent medicines for diseases.

scholarly journals PHARMACOGNOSTIC STANDARDIZATION, PRELIMINARY PHYTOCHEMICAL SCREENING AND TLC FINGERPRINTING OF THE BARK OF CASCABELA THEVETIA L.

2019 ◽  
pp. 125-130
Author(s):  
Neelutpal Gogoi ◽  
Biman Bhuyan ◽  
Trinayan Deka
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Cork Cell ◽  
Layer Chromatography

Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

scholarly journals Phytochemical Screening of the Hydro Ethanolic Extract of Brassica oleracea Var. Italica Plant Extract

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Shah M. A. ◽  
Himanshu . ◽  
Sarker M M R ◽  
Banu Afreen
Keyword(s):  
Traditional Medicine ◽  
Brassica Oleracea ◽  
Plant Extract ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Acid Group ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Pharmaceutical Industries ◽  
Potent Drug

Objective: This study was designed elucidate the Phytochemicals of the widely-used plant Brassica oleracea var. Italica. Method: Hydroethanolic extracts of Brassica oleracea var. Italica plant extract was investigated. A small portion of the hydroethanolic extracts of Brassica oleracea var. Italica was subjected to the phytochemical test using Trease and Evans and Harbourne methods to test for the presence of alkaloids, tannins, reducing sugars, saponins, terpenoids, phenols, flavonoids and Anthraquionones. Result: The phytochemical analysis revealed the presence of alkaloids, saponins, tannins, flavonoids, terpenoids, coumarins, quinines, cardiac glycosides, Xanthoproteins, glycosides, steroids, phenols, resins, carboxylic acid group in varying concentrations. The present study provides evidence that Hydro ethanolic extracts of Brassica oleracea var. Italica contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases. Conclusion: Thus, from the present study the plant leaf extracts of Brassica oleracea var. Italica showed an abundant production of Phytochemicals as secondary metabolites and they can be used in the pharmaceutical industries for producing a potent drug. The studies result of the above two plants gives a basis of its use in traditional medicine to manage ailments and disorders.

scholarly journals ANTIMICROBIAL AND ANTIOXIDANT EVALUATION OF DIFFERENT SOLVENT EXTRACTS OF MEDICINAL PLANT: FAGONIA MOLLIS DELILE

1970 ◽  
pp. 07-11
Author(s):  
S. M. ALGHANEM
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methyl Alcohol ◽  
Methylene Chloride ◽  
Test System ◽  
Klebsiella Pneumonia ◽  
Phytochemical Analysis ◽  
Alcohol Extract ◽  
Medicinal Uses

The present study was aimed to evaluation of the medicinal uses of Fagonia mollis by studying some active constituents, antioxidants and antimicrobial activities. Fagonia mollis Delile was collected from different sites from Tabuk, Saudi Arabia. The phytochemical analysis of the aerial parts of F. mollis indicated that the plant is rich in secondary compounds. F. mollis exhibited the highest content of tannins and saponins (19.9±1.68 and 16.8±1.32, mg/g D.W., respectively), followed by phenolics (11.6±0.72 mg/g D.W.), flavonoids (8.6±0.82 mg/g D.W.) and then alkaloids (5.6±0.64 mg/g D.W.). In the DPPH test system, the crud extract of F. mollis, with an IC50 value of 1.51 mg ml?1, but higher than that of the positive control catechol (0.37 mg ml?1). In the present study the petroleum ether and methylene chloride extracts of F. mollis inhibit all pathogenic bacteria with different rates. Ethyl acetate extract has no effect on both Escherichia coli and Klebsiella pneumonia, while the acetone inhibited all the bacteria except Escherichia coli and Staphylococcus aureus. Methyl alcohol extract of plant on tested bacteria proved a broad spectrum antimicrobial potential. On the other hand, petroleum ether extract inhibited the growth of Candidia albicans and Mucor spp (8 and 9.3 mm, respectively), but has no effect on the Aspergillus fumigatus and A. niger. The methylene chloride, Ethyl acetate and methyl alcohol extracts had no antifungal activities against all the pathogenic fungi. Acetone extract inhibited the growth of A. fumigatus (10.2mm) but has no effect on the others. The above results revealed that F. mollis have an excellent anti-bacterial activity and can be used for disease therapy.

The Antioxidant Activity and Phytochemical Analysis of Medicinal Plant Veronica Biloba

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
AMIR HASSAN ◽  
Himayat Ullah ◽  
Muhammad Israr
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Continuous Process ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Analysis ◽  
Uniform Size ◽  
Health Related

A medicinal plant veronica genus has 450 well known species and found across both temperate and hemisphere region their 26 species are endemic and known in a total of 79 popular species and are widely utilized throughout the world due to important biological activities. In this study fully powdered uniform size specie veronica biloba plant taken in porous bag were manually subjected to soxhlet hot continuous process for cyclization of extraction using ethanol (300 mL) a concentrated dried extract obtained after solvent evaporation. Furthermore, liquid-liquid extracted fractions as water, dichloromethane, n-hexane, and ethyl acetate yields results founds polar fraction with highest percentage (water 47.51 %). The phytochemical screening of veronica biloba has shown all major compounds entirely present in extracts. One of the primitive phenolic compound flavonoid is present in plant and show potency towards antioxidants. All the extracted fractions of plant showed excellent antioxidant activity using a stable DPPH (1,1-diphenyl-2-picryl-hydrazyl) method at concentration (range from 31.25 to 500µg/mL). Primary our ethyl acetate extract fraction showed highest inhibition potential at IC50 = 1.70±0.05µg/mL which is much closer to a standard positive control Propyl gallate showed IC50 = 1.6±0.05µg/mL percent potential. The purification and isolation of these extract is important which can provide us help in novel antioxidants discovery also natural antioxidants currently in cosmetics products, food and therapeutics health related products significantly demanded because they are very effective, efficient and harmless as compared to synthetic one.

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