scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF BIOACTIVE CONSTITUENTS IN THE ETHANOLIC EXTRACT OF EMPTY FRUIT BUNCHES

2020 ◽  
Vol 4 (2) ◽  
pp. 70-74
Author(s):  
Sameer M. Al- Al-Gorany ◽  
Saba Z. AL-Abachi ◽  
Abdulwadood I. Arif ◽  
Entesar E. Aboglida ◽  
Entesar H. Al_Abdeli
Keyword(s):  
Mass Spectra ◽  
Acid Hydrazide ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Powdered Sample ◽  
Bioactive Constituents ◽  
Empty Fruit Bunches ◽  
Ms Analysis ◽  
Phytochemical Compounds

For thousands of years plants have been an essential medicinal source with qualities. Empty fruit bunches (EFB) have medicinal values. Ten grams of powdered sample was extracted with 50 mL ethanol overnight and filtered through ash less filter paper, this plant’s ethanol extract has been analyzed using Gas Chromatography–Mass Spectrometry (GC-MS), while the compound mass spectra contained in the extract has been matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis provided different peaks determining the presence of eight different phytochemical compounds namely10-Undecenoic acid, Propanoic acid, Cyclopentane acetic acid hydrazide,1,4-Cyclohexanedicarbonitrile.The compounds were identified by comparing their retention time and peak area with literature and by interpreting the mass spectra. Many of them have antioxidant, anti-inflammatory, antimicrobial, antifungal, antitumor, antiviral, anticonvulsant, analgesic and antidiabetic properties.

Identification of Secondary Metabolites from the Ethanol extract of the leaves of Bauhinia tomentosa by GC-MS Analysis

2021 ◽  
pp. 2335-2341
Author(s):  
R. Balabhaskar ◽  
K. Vijayalakshmi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Mass Spectra ◽  
Ethanol Extract ◽  
Alpha Tocopherol ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Gas Chromatography-Mass Spectrometry (GC-MS) is normally used for direct analysis of chemical components existing in herbal medicines. The medicinal plants are having numerous bioactive components which are identified even at less than 1ng by using GC-MS or LC-MS analysis. The aim of this study is to identify the secondary metabolites present in the leaves of B. tomentosa using gas chromatography-mass spectrometry (GC-MS) analysis. In the present study the ethanol extract of the leaves of Bauhinia tomentosa has been subjected to GC-MS analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of 14 secondary metabolites. These compounds were identified by comparing their retention times and peak areas with those from the literature and by interpretation of the mass spectra. The major secondary metabolites were DL-.alpha.-tocopherol (14.84%), 2-[(trimethylsilyl oxy]-, methyl ester, 1-alpha,2-alpha.-epoxy-1-beta-methylcholesta-4,6-dien-3-one (12.93%), pentacosenoic acid (12.71%), phytol (10.28%), Ethyl Isoallocholate (8.197%), Spirost-8-en-11-one-3-hydroxy-,(3-beta,5 alpha,14 beta,20 beta, 22 beta,25R)-(8.162%), Urs-12-en-28-ol (6.675%), 1-Octadecyne (5.702%) and Cholest-8-en-3-beta-ol,Acetate (5.426%). The compounds having area less that 5% were considered of no significance. These findings suggest that the presence of these secondary metabolites may be the cause for the properties exhibited by Bauhinia tomentosa. Thus, presence of various bioactive compounds justifies the use of the leaf for various ailments by traditional practitioners.

scholarly journals Identification of Chemical Compounds from the Ethanolic Extract of the Bark of Bauhinia tomentosa L. By GC-MS Analysis

2016 ◽  
Vol 8 (03) ◽  
Author(s):  
Gopalakrishnan S. ◽  
Vadivel E.
Keyword(s):  
Mass Spectra ◽  
Skin Diseases ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Microbial Infections ◽  
Phytochemical Constituents ◽  
Major Chemical

The bark of Bauhinia tomentosa L. is used wildly used for the treatment of varies ailments such as inflammation, wound, dysentery, skin diseases and for microbial infections. In the present study the ethanolic extract of the bark of Bauhinia tomentosa L. has been subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of fourteen phytochemical constituents, These compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The major chemical constituents are 4-(Benzyloxy)-1-methoxy-2(3,7-dimethylocta-2,6-dienyl)-benzene (10.88%), Levodopa (6.39%) and (9E, 12E) - Methyl octadeca-9,12-dienoate (5.68%) which possess many biological activities. Hence these studies pave a platform to screen many bioactive components for wound healing.

scholarly journals GC-MS ANALYSIS OF BIOACTIVE CONSTITUENTS OF JASMINE FLOWER

2018 ◽  
Vol 4 (1) ◽  
pp. 52-59
Author(s):  
Hesham Hussein Rassem ◽  
Abdurahman Hamid Nour ◽  
Rosli Mohammed Yunus
Keyword(s):  
Phthalic Acid ◽  
Mass Spectra ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Microwave Assisted ◽  
Flower Extract ◽  
Ms Analysis ◽  
Traditional Practitioners ◽  
Comparison Of Mass Spectra

Gas Chromatography-Mass Spectrometry (GC-MS) the best technique to identified the compounds of essential oils by comparison of mass spectra data obtained from the sample with that taken from pure commercially available standards injected under the same conditions. To characterize the chemical constituents of Hibiscus Flower using GC-MS, the shade dried flower powder was extracted with methanol by using Microwave-assisted Hydrodistillation (MAHD). The GC-MS analysis provided different peaks determining the presence of ten compounds. These compounds havebiological activity namely 2-Phenylthiolane (57.31%), Cyclohexene, 3-ethenyl- (25.91%), Acetaldehyde (12.70%), N-Methylallylamine (9.99%),  ropanamide (6.79%) and Phthalic acid, bis (7-methyloctyl) ester (5.21%). From the results, it can be concluded that Jasmine flower extract shows the presence of 10 phytocompounds. The presence of various bioactive compounds justifies the use of the jasmine flower for various ailments by traditional practitioners.

scholarly journals Quantitative Phytochemical Composition and Bioactive Constituents of Ethanolic Extract of Phyllanthus amarus (schum. et thonn) Leaves

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
E. O. Oshomoh ◽  
O. Uzama-Avenbuan
Keyword(s):  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Total Content ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Phyllanthus Amarus ◽  
Bioactive Constituents ◽  
Phytochemical Composition ◽  
Ms Analysis ◽  
Traditional Practitioners

Herbs are used in the treatment of several human ailment and diseases. The quantitative phytochemical composition, bioactive constituents of ethanol extract of Phyllanthus amarus leaves were determined. The quantitative phytochemical analysis of ethanol extract of Phyllanthus amarus found to be present in this study includes phenol, flavonoid, saponin, tannin and alkaloid.  The total content of phenol is 73.163µg/ml, flavonoid is 137.27µg/ml, tannin is 3.581µg/ml, alkaloid is 30.1 % and saponin is 34.822 ppm. The GC-MS analysis of bioactive constituents of the ethanol extract of Phyllanthus amarus leaves resulted in 44 compounds which have diverse use.  The various bioactive compounds present in P. amarus confirm its uses for the treatment of various ailments and diseases by traditional practitioners.  

GC-MS analysis and Mitochondrial Functionality Potential of the Fruits ofTetrapleura tetraptera By Cupric Reducing Antioxidant Capacity Assay

2020 ◽  
Vol 19 (1) ◽  
pp. 338-347
Author(s):  
Chinwe S. Alaribe ◽  
Akolade R. Oladipupo ◽  
Osamede Ojo-Nosakhare ◽  
Omotayo Kehinde ◽  
Adeniyi S. Ogunlaja
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
Antioxidant Capacity ◽  
Vaccenic Acid ◽  
Octadecenoic Acid ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Degenerative Disorders ◽  
Tetrapleura Tetraptera

The use of antioxidants has emerged as a promising therapeutic strategy for the management of mitochondrial dysfunction and other oxidative stress-related degenerative pathologies. Tetrapleura tetraptera is a well-known tree and its fruit is applied traditionally as spice and in the preparation of remedies for different ailments. In this study, the gas chromatography-mass spectrometry (GC-MS) analysis and mitochondrial functionality potential of T. tetraptera fruits were investigated. GC-MS was used to detect compounds in then-hexane and ethanol extracts of T. tetraptera fruits. Cupric reducing antioxidant capacity (CUPRAC) assay was used to evaluate the mitochondrial functionality potential of the ethanol extract. GC-MS analysis revealed the presence of six compounds in then-hexane extract of T. tetraptera fruits. These compounds were detected in trace quantities, the most abundant being 2,3-dimethyl-3-buten-2-ol (0.04%). The compounds: cis-vaccenic acid (35.37%), n-hexadecanoic acid (28.09%), 6-octadecenoic acid (25.21%), and octadecanoic acid (11.33%) were identified in the ethanol extract of the fruits. Consequently, the ethanol extract was subjected to CUPRAC assay. The ethanol extract exhibited a concentration-dependent high cupric reducing capacity, returning CUPRAC values in the range of 0.090 to 0.403 at the concentration of 10 – 80 μg/ml. This activity was comparable to that of the positive control, naringenin, which showed CUPRAC values of 0.059 – 0.378 at the same concentrations. These results indicate that T. tetraptera fruits possess good antioxidant property as evaluated by other related antioxidant assays. This could be attributed to a synergistic effect of the phytochemical constituents. Hence consumption of T. tetraptera fruits could be beneficial for the prevention of mitochondrial dysfunction and other oxidative stress-related degenerative disorders. Keywords: Tetrapleura tetraptera, mitochondrial dysfunction, degenerative disorders, CUPRAC, GC-MS, Nigeria

scholarly journals Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 90-94
Author(s):  
P Sasikala ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
M Padma ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fine Powder ◽  
Ethanol Extract ◽  
Taraxacum Officinale ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Biological Compounds ◽  
Phytochemical Compounds ◽  
Medicinal Properties ◽  
Phytochemical Constituents

Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds. Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis. Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial  and antidiabetic compounds. Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various  medicinal  properties to cure dangerous diseases and disorders. Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds.

scholarly journals GC-MS ANALYSIS OF BIO-ACTIVE COMPOUNDS FROM THE ETHANOLIC EXTRACT OF BALIOSPERMUM MONTANUM (WILD.) MUELL. ARG.

2018 ◽  
Vol 5 (1) ◽  
pp. 42-45
Author(s):  
Reshma K ◽  
Baluprakash T ◽  
Abdul Kaffoor H ◽  
Venkatachalapathi A ◽  
Arumugasamy K
Keyword(s):  
Methyl Ester ◽  
Medicinal Plant ◽  
Bioactive Compounds ◽  
Mass Spectra ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Ethanolic Extract ◽  
Acid Methyl ◽  
Pharmacological Studies ◽  
Phytochemical Compounds

Baliospermum montanum (Wild.) Muell. Arg., (Euphorbiaceae) is a well known medicinal plant which is used in treatment of various diseases. The present study was focussed on the separation and investigation of the phytochemical compounds from ethanolic extract by GC-MS technique. The mass spectra of thecompounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. The ethanolic extract revealed the presence of 30 bioactive compounds. The major and minor phytochemical compounds are 2,15-Dithia[3](9,10)anthracenol[3](2,6)pyridinophane,1,2,3,4-tetrahydro showed the highest peak 9.32% followed by phytol-9.08%, Neophytadiene-7.38%, 8,11-Octadecadienoic acid, methyl ester (CAS)-7.06%, 4-ethyl-6-[2-(methoxycarb onyl)ethenyl]-7-[2-(methoxycarbonyl)ethyl]1,3,5,8-tetramethyl-2-vinylporphyrin-6.72%, 2-pentafluorophenylpropanal-6.43%,4,4'-Isopropylidene-bis-(2-cyclo hexyl phenol)-5.91% etc.. Further pharmacological studies are needed to find out the medicinal aspect of these compounds.

BRINE SHRIMPS LETHALITY TEST OF ETHANOL EXTRACT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE FRACTION OF BLIGHIA SAPIDA

2020 ◽  
pp. 208-212
Author(s):  
ADEKOLA MB ◽  
AREOLA JO ◽  
ORIYOMI OV ◽  
APATA JT ◽  
APALOWO OE ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Blighia Sapida

Objective: The objective of the study was to evaluate brine shrimp lethality of Blighia sapida stem-bark extract and its fractions and identify the bioactive constituents in the ethyl acetate fraction (EAF) using gas chromatography-mass spectrometry (GC-MS) technique. Methods: The ethanol extract (EE) and its fractions were subjected to lethality assay, and GC-MS analysis of EAF was carried out. Results: The lethality test showed a concentration-dependent mortality rate in the brine shrimp nauplii for the EE and its fractions. GC-MS analysis of EAF of the extract revealed the existence of 13 peaks of the GC-MS chromatogram with only one prominent compound, n-hexadecanoic acid (peak area of 10.13%). Conclusion: The result revealed the presence of 13 bioactive components in the EAF of the extract, the majority of which have been reported for different biological activities, hence, justifies the use of the plant in the treatment and management of different diseases ethnomedicinally.

Preliminary phytochemical screening, antimicrobial potentials and GC-MS analysis of Secamone afzelli Rhoem (Asclepiadaceae) leaves extracts

2020 ◽  
pp. 3728-3738
Author(s):  
Oladapo E. Oyinloye ◽  
Olumuyiwa. S. Alabi
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Soxhlet Apparatus ◽  
Ethanol Extracts ◽  
Tract Infections ◽  
Gastro Intestinal Tract

Secamone afzelli is used ethno-medicinally as remedies for stomach, colic and gastro-intestinal tract infections. This study screened the hexane, ethyl acetate and ethanol extracts of S. afzelli leaves for phytocompounds, antimicrobial activity and identified bioactive compounds in the most active extract using Gas Chromatography Mass Spectrometry (GC-MS) analysis. S. afzelli (200mg) leave was extracted by soxhlet apparatus using three solvents (hexane, ethyl acetate and ethanol) and the phytochemicals determined. Antimicrobial susceptibility and minimum inhibitory concentrations (MICs) was determined by agar-diffusion and agar-dilution methods respectively against selected resistant bacterial and fungal clinical isolates. The most active of the three crude extracts was analysed by GC-MS for bioactive compounds identification. Presence of carbohydrates, anthraquinone, glycosides, proteins, tannin, phenolic compounds and steroids were determined. The three extracts (hexane, ethyl acetate, and ethanol extracts) had antibacterial (Range of inhibition zone = 10 to 18 mm and MICs 12.5 to 200 mg/mL) but no antifungal activity. The ethanol extract was the most active with 29 bioactive compounds including piperazine, phytol, leucine methyl ester, N-methyl-N-(1-methylethyl)- 1Pentanamine, 7-propyl-pyrrolizin-1-thione and 4,5-dimethylnonane. The antibacterial activity and arrays of bioactive compounds detected in S. afzelli leaves suggest that the plant may be a good source of novel antimicrobial compounds.

scholarly journals GC-MS Analysis of Bioactive Compounds and Phytochemical Evaluation of the Ethanolic Extract of Gomphrena globosa L. Flowers

2020 ◽  
Vol 10 (2) ◽  
pp. 53-58
Author(s):  
Azmat Unnisa Esmat ◽  
Sirisha Mittapally ◽  
Safiya Begum
Keyword(s):  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Pharmacological Activities ◽  
Ms Analysis ◽  
Gomphrena Globosa ◽  
Whole Plant ◽  
Docosanoic Acid ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents ◽  
Anti Inflammatory

Gomphrena globosa (L.) has high medicinal values. All parts are been used as folk medicines. The extract of whole plant / flowers has shown different phytochemical constituents with various clinical properties. These phytochemicals recognised are responsible for various activities such as anti-inflammatory, anticancer, antibacterial, analgesic and cytotoxic. The ethanolic extract of Gomphrena globosa L. flowers of biochemical test indicates the presence of sterols, triterpenoid, tannins, phenols and flavonoids, and on GC-MS analysis it has shown 11 phytochemical compounds with different pharmacological activities. The major bioactive compounds are Docosanoic Acid, Docosyl Ester (25.404%) and Hexatriacontane (24.324%), has proven anti-inflammatory activity. Keywords: Gomphrena globose L., phytochemical constituents, GC-MS and anti-inflammatory.

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