Evaluation of the Anti-ulcer Effect of Extra Virgin Avocado (Persea americana) Oil in Rats

Peptic ulcer disease is a gastrointestinal disorder of high mortality and morbidity. Therapy of this disease condition with conventional drugs presents therapeutic challenge due to incidents of unwanted side effects, and the high cost of these medications. The use of products from natural sources will provide useful alternatives due to their minimal side effects, being inexpensive, and readily available, especially in resource-challenged nations of the continent of Africa and other developing economies. Oils derived from plants are increasingly being used in management of several disease conditions. This study aims at evaluating the antiulcer activity of extra virgin avocado oil in rodents using ethanol-induced, and indomethacin-induced ulcer models. Test group was given extra virgin avocado oil (1ml per 250g weight) for 7 days before induction of ulcer. Positive controls received omeprazole 30 mg/kg, while negative control animals were given distilled water (10ml/kg) for 7 days respectively, before induction of ulcer. The stomachs were excised and their histopathological examinations carried out. Avocado oil significantly reduced the ulcer index in both models when compared to the negative control group, and histopathological findings corroborate that the oil ameliorates ulcerations in both models. The present study has demonstrated that extra virgin avocado oil possesses significant antiulcer activity.

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Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

Journal of Clinical and Health Sciences ◽

10.24191/jchs.v1i1.5850 ◽

2016 ◽

Vol 1 (1) ◽

pp. 22

Author(s):

Nazli Zainuddin ◽

Nurul Azira Mohd Shah ◽

Rosdan Salim

Keyword(s):

Allergic Rhinitis ◽

Side Effects ◽

Coconut Oil ◽

Test Group ◽

Nasal Symptom ◽

Control Group ◽

Virgin Coconut Oil ◽

Control Groups ◽

Significant Difference ◽

And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

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Bioassay of Chick Edema Factor—1962 Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/46.3.406 ◽

1963 ◽

Vol 46 (3) ◽

pp. 406-412

Author(s):

D F Flick ◽

James Winbush ◽

Leo Friedman

Keyword(s):

Collaborative Study ◽

Test Group ◽

Negative Control Group ◽

Negative Control ◽

Pericardial Fluid ◽

Fluid Volume ◽

Control Group ◽

Fatty Material ◽

Edema Factor ◽

The Mean

Abstract The lower limits of sensitivity of the method by Douglass and Flick for the bioassay of the chick edema factor were more clearly delineated by studies this year in which toxic fatty material (TFM) was fed in duplicate trials at 0.00, 0.25, 0.50, 0.75, and 1.00 g per 16 g cottonseed oil, USP. Nine laboratories collaborated in these studies. Results indicated that the following criteria to establish presence of chick edema factor is valid: "t" value exceeds + 1.3, the mean log (pericardial fluid volume X 100) of the negative control group is 1.1460 or less, and the mean log (pericardial fluid volume X 100) of the test group is 1.1461 or more.

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Uji Daya Hambat Ekstrak Ikan Nike (Awous melanocephalus) Terhadap Pertumbuhan Bakteri Fusobacterium nucleatum

e-GIGI ◽

10.35790/eg.v9i2.34982 ◽

2021 ◽

Vol 9 (2) ◽

pp. 238

Author(s):

Stevia E. Nonutu ◽

Damajanty H. C. Pangemanan ◽

Christy N. Mintjelungan

Keyword(s):

Side Effects ◽

Treatment Options ◽

Antibacterial Properties ◽

Fusobacterium Nucleatum ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Control Group Design ◽

Group Design

Abstract: One of the treatment options of periodontal abscess caused by Fusobacterium nucleatum is administration of antibiotics. However, long-term antibiotics consumption can cause negative side effects. Therefore, alternative treatments that have low side effects and easy to be obtained are needed. Nike fish (Awaous melanocephalus) is one of the endemic fish of North Sulawesi province which has antibacterial properties. This study was aimed to evaluate the inhibition effect of nike fish extract on the growth of Fusobacterium nucleatum. This was a true experimental study with a posttest only control group design. We used modified Kirby-Bauer method with filter papers. Ciprofloxacin was used as the positive control and aquadest as the negative control. Extract of nike fish and stock of pure bacteria Fusobacterium nucleatum were prepared. The results showed that the average diameters of the inhibition zones formed in the nike fish extract after three repetitions, were as follows: for extract concentration of 12.5% was 2.91 mm; 25% was 4.16 mm; 50% was 8.41 mm; and 100% was 9.58 mm. In conclusion, nike fish extract (Awaous melanocephalus) at concentrations of 50% and 100% had a weak inhibitory effect (Himedia category) on the growth of Fusobacterium nucleatum meanwhile at concentrations of 12.5% and 25% there was no activity of zone of inhibition.Keywords: extract of nike fish (Awaous melanocephalus); Fusobacterium nucleatum; inhibitory effect Abstrak: Salah satu opsi pengobatan abses periodontal yang disebabkan oleh bakteri Fusobacterium nucleatum yaitu dengan penggunaan antibiotik namun mengonsumsi antibiotik jangka panjang dapat menimbulkan efek samping negatif. Oleh karena itu, diperlukan pengobatan alternatif yang memiliki efek samping rendah serta mudah didapat. Ikan nike merupakan salah satu ikan endemik Provinsi Sulawesi Utara yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak ikan nike (Awaous melanocephalus) terhadap pertumbuhan bakteri Fusobacterium nucleatum. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan paper disk. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak ikan nike dan stok bakteri murni Fusobacterium nucleatum. Hasil penelitian menunjukkan bahwa rerata diameter zona hambat yang terbentuk pada ekstrak ikan nike setelah tiga kali pengulangan yaitu untuk konsentrasi 12,5% sebesar 2,91 mm; 25% sebesar 4,16 mm; 50% sebesar 8,41 mm; dan 100% sebesar 9,58 mm. Simpulan penelitian ini ialah ekstrak ikan nike (Awaous melanocephalus) pada konsentrasi 50% dan 100% memiliki daya hambat kategori lemah (Himedia) terhadap pertumbuhan bakteri Fusobacterium nucleatum sedangkan pada konsentrasi 12,5% dan 25% dikategorikan tidak terdapat aktivitas zona hambat. Kata kunci: ekstrak ikan nike (Awaous melanocephalus); Fusobacterium nucleatum; daya hambat

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Efficacy of Ayarij Faiqura in Qarah-e-Hazmiya - Randomized controlled Clinical study

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v17i1.35291 ◽

2018 ◽

Vol 17 (1) ◽

pp. 112-117

Author(s):

MA Siddiqui ◽

Abdul Quavi ◽

Mohd Aleemuddin Quamri

Keyword(s):

Medical Science ◽

Test Group ◽

Burning Sensation ◽

Control Group ◽

Test Drug ◽

Ulcer Disease ◽

Control Drug ◽

Pain Abdomen ◽

The Difference ◽

And Control

Background and Objective: Qarahe Hazmiya (Peptic ulcer disease) is one of the commonest ailment of Gastro intestinal tract, reported to affect around 4% of the population and 10% of people developed it at some point in their life. Etiopathology of the disease is considered as multi factorial mostly treated with medications and rarely with surgery. Several Unani formulation have been indicated effective in Qarahe Hazmiya, one of such formulation has been selected to evaluate its efficacy and to validate the same on scientific parametersMethods: This study was conducted as a single blind, randomized standard control clinical trial on 28 patients (n=20) in test and (n=8) control groups. Test group received Ayarij Faiqura 5gm and control group received Pantoprazole 40 mg, orally in empty stomach for 45 days. The study outcome was assessed as the difference in pre and post treatment through subjective and objective parameters.Result: The test drugs revealed statistically significant improvement in burning sensation in abdomen (p<0.0001), pain abdomen (p<0.0001) as subjective parameters in comparison to control group where as objectivecally endoscopic report showed significantly improvement (p<0.0196) in comparison to control group with respect to the healing of ulcer / erosions.Interpretation and conclusion: The study revealed that the test drug had good response in controlling burning sensation and pain abdomen, along with healing of ulcer / erosions in comparison to control drug. No side effect or toxicity was observed during and or after the study. Thus, it could be concluded that the test drug was found to be safe and effective without any adverse effect.Bangladesh Journal of Medical Science Vol.17(1) 2018 p.112-117

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BLACK BANGLE (ZINGIBER OTTENSII VAL.) RHIZOME AND KATUK LEAVES (SAUROPUS ANDROGYNUS L. MERR) EXTRACT COMBINATION PROTECTIVE ROLE ON ADIPOSE TISSUES HISTOLOGIC PROFILE OF HIGH-FAT AND CARBOHYDRATE DIET-INDUCED OBESE MALE RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11s1.26613 ◽

2018 ◽

Vol 11 (13) ◽

pp. 225

Author(s):

Sulaeman A ◽

Patonah Patonah ◽

Negara Gg ◽

Negara Gg

Keyword(s):

Test Group ◽

Negative Control Group ◽

Protective Role ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

High Fat ◽

Adipose Tissues ◽

Obese Male

Objective: The effect of Zingiber ottensii Val. rhizome and Sauropus androgynus L. Merr leaves extract combination was investigated using histologic profile of adipose tissues in obese male rats induced by high-fat and carbohydrate diets.Methods: This was a preventive study, conducted for 42 days by simultaneous administration of diets and extracts administration. The subjects were divided into 8 groups. All groups except negative control group were fed with high-fat and carbohydrate diets. Orlistat, metformin, and curcumin were used as contrast.Result: The phytochemical screening of Z. ottensii Val. rhizome extract showed the presence of flavonoids, saponins, and triterpenoids, meanwhile S. androgynus L. Merr leaves extract presented flavonoids, tannins, saponins, steroids, and triterpenoids. The results showed tissues histological differences in all test group compared with positive control. The most effective combination dose for bangle and katuk leaves extract in protecting adipose tissue was 100 mg/Kg:100 mg/Kg body weight.Conclusion: The combination of black bangle and katuk leaves extract showed a protective role, demonstrated by adipose tissues histologic profile.

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Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

Brazilian Dental Science ◽

10.14295/bds.2020.v23i3.1895 ◽

2020 ◽

Vol 23 (3) ◽

Author(s):

Saeedeh Galledar ◽

Robab Farhang ◽

Malek Abazari ◽

Parisa Negahdar

Keyword(s):

Root Canal ◽

Test Group ◽

In Vitro Study ◽

Negative Control ◽

Vitro Study ◽

Control Group ◽

Dye Penetration ◽

Gutta Percha ◽

Significant Difference

Objective: Proper apical seal plays an important role in the success of root canal treatment. The most common cause of failure of root canal therapy is known as the lack of adequate apical seal. The aim of this in vitro study was to compare the apical microleakage of MTA Fillapex, , and Endofill sealers using dye penetration method. Material and Methods: In this in vitro study, 72 single-rooted extracted human teeth were selected. The teeth were randomly divided into three experimental groups of 20 and two positive and negative control groups of 6. The canals were prepared by step-back technique and then filled with gutta-percha and one of the sealers mentioned. In the positive control group, the canals were filled with gutta-percha without sealer, and in the negative control group, the canals were prepared but not filled. The teeth were immersed in 2% methylene blue dye for 72 hours. The teeth were then cut longitudinally and the level of dye penetration was measured under a stereomicroscope. Data were analyzed by SPSS ver. 19 software, ANOVA and Bonferroni post-hoc tests. Results: The mean level of dye penetration in the Endofill test group was significantly higher than that in the and MTA Fillapex test groups. While, the observed difference between and MTA Fillapex groups was not statistically significant (p<0.05). Conclusion: The results of this study showed that and MTA Fillapex sealers did not show any significant difference in apical seal properties. However, their sealing strength was significantly greater than Endofill sealer.KeywordsAH26 sealer; Endofill; MTA Fillapex; Microleakag

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The Antimalarial Activity of the Water Extract of Simpur (Dillenia Indica L) Leaves against Plasmodium Berghei in Mice

Majalah Obat Tradisional ◽

10.22146/mot.38626 ◽

2019 ◽

Vol 24 (1) ◽

pp. 28

Author(s):

Hafrizal Riza ◽

Andhi Fahrurroji ◽

Supriyanto Supriyanto ◽

Arif Wicaksono

Keyword(s):

Plasmodium Berghei ◽

Antimalarial Activity ◽

Water Extract ◽

Test Group ◽

Negative Control ◽

Test Solution ◽

Test Method ◽

Control Group ◽

Dillenia Indica

Malaria, caused by the climate of the subtropical area and the forest with many rivers and immovable water, is a contagious disease that still becomes a health problem in West Kalimantan. Simpur is a plant that is used by the locals to cure malaria. Therefore, this research aims to study the antimalarial activity in vivo and the 50% inhibitory concentration (IC50) of the water extract of Simpur leaves (Dillenia indica L) against Plasmodium berghei. This research used Peter Test method that used 7 test groups based on the test solution namely positive control group that was given dihydroartemisinin+piperaquine (DHP), negative control that was given aquabidestilata and the test group that was given the water extract of Simpur leaves with various doses of 20, 40, 60, 80 and 100 mg/Kg BB of mice, which each group was given the test solution for 3 days. The result shows that the water extract of Simpur leaves could lower the parasitemia count with IC50 19,22 mg/kg BB.

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Effects of Additional Use of Bioactive Glasses or a Hydroxyapatite Toothpaste on Remineralization of Artificial Lesions in vitro

Caries Research ◽

10.1159/000510180 ◽

2020 ◽

Vol 54 (4) ◽

pp. 336-342

Author(s):

Philipp Körner ◽

Jana A Schleich ◽

Daniel B. Wiedemeier ◽

Thomas Attin ◽

Florian J. Wegehaupt

Keyword(s):

Bioactive Glass ◽

Artificial Saliva ◽

Test Group ◽

In Vitro Study ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Bioactive Glasses ◽

Initial Caries

Objectives: This in vitro study aimed to evaluate and compare the effect of two different bioactive glasses, a hydroxyapatite-containing, fluoride-free toothpaste (HTP) and a fluoride toothpaste (FTP) on the remineralization behavior of initial caries lesions. Materials and Methods: A total of 100 bovine enamel samples were randomly allocated to five groups of 20 samples each: NC = negative control group (artificial saliva); HTP = HTP group (Karex); FTP = FTP group (Elmex caries protection, 1,400 ppm); FTP + BGnano = FTP followed by Actimins bioactive glass; FTP + BGamorph = FTP followed by Schott bioactive glass. Radiographic documentation (advanced transversal microradiography; aTMR) was applied before and after all samples were exposed to a demineralizing gel for 10 days. Over a period of 28 days, samples were covered twice a day (every 12 h) with a toothpaste slurry of the respective test group or with artificial saliva in NC for 60 s and brushed with 15 brushing strokes. Samples in FTP + BGnano and FTP + BGamorph were additionally treated with the respective bioactive glass slurry for 30 s after brushing with the FTP. In the meantime, all samples were stored in artificial saliva. After 28 days, the structure of all samples was assessed again using aTMR and compared to the values measured after demineralization. The statistical evaluation of the integrated mineral loss was performed using Kruskal-Wallis test followed by a post hoc Conover test. Results: The FTP revealed the significantly highest increase of mineral content while the HTP showed the significantly lowest remineralization. Compared to artificial saliva, the use of the HTP or the combined application of FTP followed by bioactive glasses (FTP + BGnano and FTP + BGamorph) showed no significant remineralization. Conclusion: Under remineralizing in vitro conditions, brushing with 1,400 ppm FTP induced significantly more remineralization compared to storage in artificial saliva. The additional administration of both bioactive glasses as well as the substitutional brushing with an HTP resulted in significantly less remineralization compared to brushing with 1,400 ppm FTP.

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Hepatoprotective and Hematological Effects of Solanum melongena (Garden Egg), Solannum lycopersicum (Tomato) and Daucus carrots Subsp. Sativus (Carrot) Extracts against Lead Induced Toxicity in Wistar Rats

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2021/v7i230169 ◽

2021 ◽

pp. 10-18

Author(s):

Dennis Amaechi ◽

I. P. Ekpe ◽

E. D. Edet ◽

M. C. Madu

Keyword(s):

Blood Cell ◽

Wistar Rats ◽

Test Group ◽

Negative Control ◽

Control Group ◽

Acetate Solution ◽

Treatment Groups ◽

Cell Counts ◽

Group I ◽

Group Ii

Background of the Study: Lead effects were assessed by analyzing the impacts of the extract on the liver enzyme concentrations and hematology parameters. Materials and Methods: Thirty five male wistar rats weighing 85-110 g were distributed into five groups consisting of seven rats each. Group I served as control group, group II served as the test group, groups III, IV and V served as treatment groups. Lead acetate solution was given to the rats orally at a dose of 50 mg/kg body weight and 200 mg/kg of fruit extracts for 14 days. On day 15, biochemical analysis were carried out. Results: Effects of extracts showed that ALT,AST and ALP concentration in group II was observed to be significantly (p<0.05) higher than the control and treatment groups with values. The hematology results showed that lead did not cause a significant reduction in the packed cell volume, white blood cell and red blood cell counts. However, the group treated with carrot and garden egg showed slight increase in RBC and WBC count when compared with the positive and negative control groups. Conclusion: From the results above, it showed that the fruits extract have hematopoietic potentials and hence aid in the hepatoprotection of the liver of lead induced Wistar rats.

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EFEK SELENIUM OKSIKLORIDA TERHADAP AKTIVITAS IMUNOMODULATOR DARI EKSOPOLISAKARIDA Lactobacillus plantarum

BERITA BIOLOGI ◽

10.14203/beritabiologi.v19i3b.3875 ◽

2020 ◽

Vol 19 (3B) ◽

Author(s):

Fifi Afiati ◽

D. C. Agustina ◽

S. Wiryowidagdo ◽

Kusmiati Kusmiati ◽

Atit Kanti

Keyword(s):

Lactobacillus Plantarum ◽

Test Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Phagocytic Capacity ◽

Treatment Groups ◽

Group 4 ◽

Group A

Lactobacillus plantarum (LAB) is a group of lactic acid bacteria that can produce exopolysaccharide (EPS). The objective of this study was to determine the effect of adding selenium chloride oxide (SeOCl2) to the increased immunomodulatory potential of EPS L. plantarum Y-1.2. The experiment was conducted in vivo using macrophage cells of peritoneal fluid of mice induced by Staphylococcus aureus. EPS was obtained by precipitation at 4 °C. Identification test using HPLC showed that crude EPS of L. plantarum Y-1.2 containing glucose monomers for value retention times 4.198 and 4.204, crude EPS approaches the retention time of glucose BP 4.234. Testing of immuno-modulatory divided into six treatment groups. Group 1-3 as the control group, a positive control (Stimuno), negative control (crude EPS 50 mg/kg BW in mice), normal control (distilled water). Group 4-6 as the test group was given crude EPS 50 mg/kg BW in mice and selenium oxide chloride doses of 0.13 µg; 0.26 µg; 0.52 µg. The test results showed a dosage of 0.52 µg selenium chloride oxide to crude EPS of 50 mg/kg BW in mice could increase the activity and phagocytic capacity amounted to 28.06% and 28.39%

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