Prevalence of AntiHelicobacter pylori Antibodies among Students of Cihan University, Erbil

Helicobacter pylori is resident in human stomach and causes chronic disease (peptic ulcer and gastritis). The mouth and colon were both known to host a large number of microbes. This study was carried out to investigate the seroprevalence of H. pylori infection among Cihan University students. A total of 197 blood samples were collected from the students (53 females and 144 males) from 13 departments of Cihan University, Erbil, and tested for anti-H. pylori antibodies, using rapid immunochromatography assay. Among 197 students tested, 44 (22.3%) showed positive reaction for H. pylori, 32 males and 12 females. It was non-significantly higher among students with ages ranged between 29 and 32 years old. Twenty-one of infected students were using tap water for drinking. Twenty-six (59.1%) of positive students experienced no symptoms and 18 (40.9%) were symptomatic (13.63% epigastric pain and 27.2% abdominal pain).

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Helicobacter pylori infection in Iran: demographic, endoscopic and pathological factors

BMC Gastroenterology ◽

10.1186/s12876-021-01931-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Seyedeh Amineh Hojati ◽

Sara Kokabpeyk ◽

Salma Yaghoubi ◽

Farahnaz Joukar ◽

Mehrnaz Asgharnezhad ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Epigastric Pain ◽

Tap Water ◽

Clinical Manifestations ◽

Gastrointestinal Symptoms ◽

Common Symptom ◽

Pathological Findings ◽

Significant Difference ◽

H Pylori

Abstract Background Helicobacter pylori (H. pylori) infection is the most important risk factor for gastritis and peptic ulcer. However, factors other than H. pylori are involved in its pathogenesis. In the current study, we aimed to compare the clinical manifestations and endoscopic and histopathological findings of patients with and without H. pylori infection. Methods In this cross-sectional study, 233 patients with dyspepsia, referred for endoscopy, were examined regarding the presence of H. pylori infection. During an endoscopic exam, 5 biopsy specimens were taken from the stomach. The criteria for the presence of H. pylori infection was the presence and identification of bacteria in pathology. Two groups of H. pylori-positive and H. pylori-negative patients were compared regarding their demographic, endoscopic, and pathological findings. Results Of 233 patients, 154 (66.1%) were non-smokers, 201 (86.3%) were not alcohol users, and 153 (65.7%) used tap water. The most common symptom, reported in 157 (67.4%) patients, was epigastric pain. There was a significant difference between patients with and without H. pylori infection in terms of the educational status, occupational status, family history of gastrointestinal cancer, and some gastrointestinal symptoms. Also, there was a significant relationship between the endoscopic and pathological findings of patients with H. pylori. Conclusions The results of the present study revealed that H. pylori infection was not associated with sex, alcohol consumption, or non-steroidal anti-inflammatory drug use. The role of H. pylori in the pathophysiology of peptic ulcer was clarified. Also, there was a significant difference in the endoscopic and pathological findings of patients with H. pylori.

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Prevalence and factors associated with Helicobacter Pylori infection among children with sickle cell anemia attending Mulago hospital, in Uganda

10.21203/rs.2.15343/v1 ◽

2019 ◽

Cited By ~ 1

Author(s):

IDRIS SWALEH MUBIRU ◽

Phillip Gita Kasirye ◽

Heather Hume ◽

Grace Ndeezi

Keyword(s):

Helicobacter Pylori ◽

Abdominal Pain ◽

Sickle Cell ◽

Sickle Cell Anemia ◽

Epigastric Pain ◽

Pneumococcal Vaccination ◽

Helicobacter Pylori Infection ◽

Mulago Hospital ◽

Factors Associated ◽

H Pylori

Abstract Background Children with sickle cell anemia (SCA) have a high predisposition to a range of infections and gastrointestinal disorders. Studies of children living in low income countries have shown high levels of infection with Helicobacter Pylori (H. Pylori), however, there are no reports in children with sickle cell anemia.Objectives We aimed to describe the prevalence and factors associated with Helicobacter pylori infection among children with sickle cell anemia at Mulago Hospital Sickle Cell Clinic, in Uganda.Methods A cross sectional design was employed to consecutively enroll 369 children with confirmed sickle cell anemia aged 5-18 years, attending the Mulago hospital sickle cell clinic. Assessments included; abdominal pain (current, recurrent, generalized or epigastric), dyspeptic symptoms, other relevant medical and social histories. Blood and stool samples were collected and an antigen test (test kit SD Bioline) was carried out on the latter to determine H.pylori infection. Ethical approval, consent and assent (for children above 8 years of age) were obtained. Data was entered using EPI DATA version 3.1 and analysed using STATA version 12.0. H. pylori prevalence was determined as a proportion of children with a positive stool test expressed as a percentage. Adjusted Odds ratios (aOR) were used to determine factors associated with H.pylori infection.Results H.pylori infection was detected in 47.7% (176/379), 95% CI (42.9-53.1) of the cases. Having epigastric pain was independently associated with H.pylori infection; aOR 2.22, 95%CI: (1.1 – 4.6), p-value= 0.03 . Pneumococcal vaccination aOR 0.41, 95% (CI 0.2-0.9; p=0.019 ) or H.pyroli combination therapy aOR 0.183, 95% (CI 0.1- 0.6; p=0.006 ) were protective against H.pylori. Recurrent abdominal pain did not predict H.pyroli infection. Conclusions H.pylori infection was common among children with sickle cell anemia and was independently associated with epigastric pain but not recurrent pain. Pneumococcal vaccination and combination H. pylori treatment were protective against the infection. Screening for H.Pylori should be carried out in sickle cell anaemia children with epigastric pain.

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Prevalence and risk factors of Helicobacter pylori infections in the patients suffering from acid-peptic disease at tertiary care center, Gujarat, India

International Journal of Advances in Medicine ◽

10.18203/2349-3933.ijam20183903 ◽

2018 ◽

Vol 5 (5) ◽

pp. 1250

Author(s):

Anjali K. Kukreja ◽

Himani B. Pandya ◽

Santosh Kumar

Keyword(s):

Risk Factors ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Abdominal Pain ◽

Prevalence Rate ◽

Tertiary Care ◽

Chili Pepper ◽

Care Hospital ◽

Spicy Food ◽

H Pylori

Background: Helicobacter pylori (H. pylori) infection is a medical encumber especially in the developing countries, ranging from acute inflammation to malignancy. The study is intended to explore the prevalence of H. pylori infection and also to compute its associated risk factors.Methods: Sixty two (n=62) symptomatic patients attending the tertiary care hospital, were included in the study. Two antral biopsies collected from each patient were subjected to rapid urease test and Gram staining. IgG antibodies were detected by rapid test based on the principle of Immunochromatography. To evaluate the risk factors, various determinants were asked to each patient in the form of questionnaire. Statistical analysis was done using Fisher exact test. P value < 0.05 was considered significant.Results: Prevalence rate of H. pylori infection was 24.19 % (15/62). More than half (53%) of the infected patients had complained of persistent burning abdominal pain. Prevalence rate was found highest in the patients suffering from peptic ulcer disease (66.6%, P=0.0237). Consumption of unfiltered water (P=0.007, Odd Ratio (OR)-1.06), eating spicy food, meat, fish and chili pepper (P=0.052, OR-1.01) was found the major risk factors. Addictive habits of either smoking/alcohol/tobacco (P=0.904) were not associated with infection.Conclusions: Prevalence of H. pylori infection was found low comparatively and was mainly associated with symptom of persistent burning abdominal pain and peptic ulcer. Use of unfiltered water, spicy food, chili pepper and meat undoubtedly increase the risk of acquiring H. pylori infection. Addictive habits were not significantly associated with infection.

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Helicobacter Pylori Infection in Iran: Demographic, Endoscopic and Pathological Factors

10.21203/rs.3.rs-222253/v1 ◽

2021 ◽

Author(s):

Seyedeh Amineh Hojati ◽

Sara Kokabpeyk ◽

Salma Yaghoubi ◽

Farahnaz Joukar ◽

Mehrnaz Asgharnezhad ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Tap Water ◽

Helicobacter Pylori Infection ◽

Common Symptom ◽

Significant Relation ◽

Cross Sectional ◽

Significant Difference ◽

Pathologic Findings ◽

H Pylori

Abstract Background: Helicobacter pylori infection is the most important risk factor for Gastritis and Peptic Ulcer. But other factors except H. pylori play roles in its pathogenesis. In current study we compare the clinical manifestation and endoscopic and histopathology findings in patients with and without H. pylori infection.Methods: In this cross-sectional study, 233 of patients with dyspepsia who referred for endoscopy were studied about presence of H. pylori infection. During endoscopy 5 biopsies were taken from each individual. The criteria for presence of H. pylori infection was the presence and recognition of bacteria in pathologic specimen which was done for all patient. These two groups of patient were compared with their demographic, background, endoscopic and pathologic findings. Results: From 233 cases, 154 (66.1%) cases were non-smoker, 201 (86.3%) cases were not alcohol user, 153 (65.7%) cases used tap water and the most common symptom was reported in 157 individual (67.4%) was epigastria pain. There was a significant relation between the number of family member, marital status and presence of H. pylori infection. Also there is a significant relation between endoscopic findings and pathologic findings in patients with H. pylori.Conclusions: the results of current studt demonstrated that H. pylori infection was not associated with sex, alcohol and NSAID drugs. According to the role of H. pylori in pathophysiology of peptic ulcer was clarified, also in this study there were a significant difference in endoscopic and pathological findings H. pylori.

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Haemagglutination (HA) test of Helicobacter pylori

Baghdad Science Journal ◽

10.21123/bsj.11.3.1382-1386 ◽

2014 ◽

Vol 11 (3) ◽

pp. 1382-1386

Author(s):

Baghdad Science Journal

Keyword(s):

Helicobacter Pylori ◽

Abdominal Pain ◽

Epithelial Cell ◽

Red Blood Cells ◽

Blood Cells ◽

Epigastric Pain ◽

Human Epithelial Cell ◽

Specific Receptors ◽

H Pylori ◽

Pathogenic Process

Thirty clinical isolates of Helicobacter pylori bacteria obtained from patients attending endoscopy unit of Ibn-Sena teaching hospital in Mosul . These patients were complaining from epigastric pain , dyspepsia , acidity , vomiting , abdominal pain , flatulance , heart burn and melena . The H. pylori isolates were used for Haemagglutination assay (HA) , which involves the recognition of various glycoconjugates on the surface of red blood cells . In this study sheep red blood cells has been used in (HA) assay because the sheep erythrocytes surface resemble that of human epithelial cells . The results proved by (HA) assay, the ability of H. pylori to adherence to specific receptors on the surface of Human Epithelial Cell , which is the first step in the pathogenic process .

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Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer

Revista do Hospital das Clínicas ◽

10.1590/s0041-87812000000500001 ◽

2000 ◽

Vol 55 (5) ◽

pp. 155-160 ◽

Cited By ~ 8

Author(s):

Rejane Mattar ◽

Antonio Atílio Laudanna

Keyword(s):

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Gastric Mucosa ◽

Virulent Strain ◽

Normal Population ◽

Human Stomach ◽

Caga Status ◽

H Pylori ◽

Multiple Strains

Even though the seroprevalence of H. pylori may be high in the normal population, a minority develops peptic ulcer. Colonization of the gastric mucosa by more pathogenic vacA strains of H. pylori seems to be associated with enhanced gastric inflammation and duodenal ulcer. H. pylori genotyping from positive CLOtests was developed to determine the vacA genotypes and cagA status in 40 duodenal ulcer patients and for routine use. The pathogenic s1b/ m1/ cagA genotype was the most frequently occurring strain (17/42.5%); only two (5%) patients presented the s2/ m2 genotype, the less virulent strain. Multiple strains were also detected in 17 (42.5%) patients. Multiple strains of H. pylori colonizing the human stomach have been underestimated, because genotyping has been performed from cultures of H. pylori. We concluded that genotyping of H. pylori from a positive CLOtest had the advantages of reducing the number of biopsies taken during endoscopy, eliminating the step of culturing H. pylori, and assuring the presence of H. pylori in the specimen being processed.

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Evaluation of Putative Association Between Celiac Disease and Helicobacter pylori Infection in Hyderabad, Sindh

Biological Sciences - PJSIR ◽

10.52763/pjsir.biol.sci.63.3.2020.157.162 ◽

2020 ◽

Vol 63 (3) ◽

pp. 157-162

Author(s):

Babar Aijaz Memon ◽

Sarfraz Ali Tunio ◽

Shaista Bano ◽

Abdul Nabi Jatt ◽

Ghulam Asghar Maka

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Celiac Disease ◽

Tissue Transglutaminase ◽

Blood Samples ◽

Iga Antibodies ◽

Male Patients ◽

H Pylori ◽

Health And Mortality

Celiac disease is an autoimmune mediated enteropathy caused by ingestion of gluten and affects approximately 1% of the population. It has significant deleterious effects on health and mortality. Helicobacter pylori is the major causative agent of peptic ulcer and gastric cancer. The aim of this study was to assess the prevalence of both H. pylori (HP) and celiac disease (CD) as mono and co-infections and to find out the possible association between CD and presence of HP. A total of 172 blood samples from individuals suspected of CD and HP infection were collected during March to December, 2015 from Hyderabad. Serum analyzed for presence of anti-HP antibodies for evaluation of HP infection and tissue transglutaminase IgA antibodies (tTGA) for detection of CD. The serodiagnosis data revealed that out of 172 samples tested 19.19% were positive for CD which included 6.98% (n=12) male patients, whereas 12.21% (n=21) were females. The HP was detected in 44.77% (n=77) including 22.09% (n=38) males and 22.67% (n=39) females out of the 33 CD positive cases, HP was detected in 17 (9.88%). HP in CD positive cases was found to be 6.60% in males 9.43% in females respectively. The HP prevalence in non-CD patients appeared to be high such as 18.02% and 16.86%. In conclusion, the data of the present study indicates that HP is more prevalent than CD.

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Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

10.51429/ejmm29308 ◽

2020 ◽

Vol 29 (3) ◽

pp. 59-64

Author(s):

Hanaa M. El Maghraby ◽

Samar Mohaseb

Keyword(s):

Helicobacter Pylori ◽

Epigastric Pain ◽

Deletion Mutation ◽

Rapid Urease Test ◽

Rrna Gene ◽

University Hospitals ◽

Metronidazole Resistance ◽

Urease Test ◽

Gastric Biopsies ◽

H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

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Helicobacter pyloriAnti-CagA Antibodies: Prevalence in Symptomatic and Asymptomatic Subjects in Turkey

Canadian Journal of Gastroenterology ◽

10.1155/2002/589087 ◽

2002 ◽

Vol 16 (8) ◽

pp. 527-532 ◽

Cited By ~ 13

Author(s):

M Fatih Abasiyanik ◽

Ersan Sander ◽

Barik A Salih

Keyword(s):

Gastric Cancer ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Chronic Gastritis ◽

Molecular Weights ◽

Cancer Antigens ◽

Gastroduodenal Disease ◽

H Pylori ◽

Asymptomatic Subjects

BACKGROUND: Several reports have shown the prevalence of anti-CagA antibodies to be associated with the development of peptic ulcer diseases, while others have indicated that there is no such association.AIM: To examine the prevalence of antibodies to CagA and otherHelicobacter pyloriantigens in symptomatic and asymptomatic subjects in Turkey.SUBJECTS AND METHODS: Sixty-six symptomatic subjects, 16 to 74 years of age, were examined forH pyloriby biopsy-based tests and ELISA. One hundred nineteen asymptomatic subjects, 20 to 65 years of age, were also tested serologically for the presence ofH pylori. Samples from both groups that were found to be positive forH pyloriby ELISA were then tested by immunoblotting.RESULTS: Fifty-four (82%) symptomatic subjects and 76 (64%) asymptomatic subjects were found to beH pylori-positive by ELISA. Samples from 30 symptomatic subjects who were found to beH pylori-positive by ELISA were analyzed by immunoblotting. Antibodies to CagA (116 kDa) antigen were detected in immunoblots of 11 of 14 (79%) with chronic gastritis, 12 of 13 (92%) with duodenal ulcer and three of three (100%) with gastric cancer. Antigens of the following molecular weights were also detected in these 30 subjects: 89 kDa (VacA) in 21 (70%), 37 kDa in 21 (70%), 35 kDa in 19 (63%), 30 kDa in 27 (90%) and 19.5 kDa in 19 (63%). Immunoblots of 40 ELISA-positive asymptomatic subjects showed that 33 (83%) had antibodies to CagA antigen, 26 (65%) to VacA antigen, 30 (75%) to a 37 kDa antigen, 30 (75%) to a 35 kDa antigen, 39 (98%) to a 30 kDa antigen and 36 (90%) to a 19.5 kDa antigen.CONCLUSIONS: Antibodies to CagA antigen were prevalent in both groups, regardless of the presence of gastroduodenal disease.

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Epidemiology of upper gastrointestinal bleeding and Helicobacter pylori infection: review of 3,488 Thai patients

Asian Biomedicine ◽

10.5372/1905-7415.0901.373 ◽

2017 ◽

Vol 9 (1) ◽

pp. 87-93

Author(s):

Sakolwan Suchartlikitwong ◽

Kamolyut Lapumnuaypol ◽

Rungsun Rerknimitr ◽

Duangporn Werawatganon

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Gastrointestinal Bleeding ◽

Upper Gastrointestinal Bleeding ◽

Helicobacter Pylori Infection ◽

Exclusion Criteria ◽

Common Causes ◽

Mucosal Lesions ◽

H Pylori ◽

Upper Gastrointestinal

Abstract Background The current epidemiology of upper gastrointestinal bleeding (UGIB) in Thailand is poorly understood and the reported prevalence of Helicobacter pylori infection is outdated. Objectives To investigate the etiologies of UGIB and prevalence of H. pylori infection in Thailand, including its association with UGIB. Methods We retrieved information regarding patients attending the endoscopic unit of King Chulalongkorn Memorial Hospital from June 2007 to January 2013. A database search using keywords “upper gastrointestinal bleeding” and “iron deficiency” was used. From 4,454 diagnoses, after exclusion criteria, 3,488 patients (2,042 male (58.5%) and 1,446 female (41.5%); mean age 63.3 ± 15.94 years, range 13–103 years) were included. Results The three most common causes of UGIB were peptic ulcer (38.2%), nonulcer-mucosal lesions (23.4%), and esophageal-related causes (20.4%). The 5 year-incidence of H. pylori was 25%–30%. The overall prevalence was 27%. The prevalence of H. pylori infection was found to decrease with age from 43.8% at <40 years to 21.7% at >79 years old. H. pylori infection was significantly associated with duodenal and gastroduodenal ulcers. Cirrhosis and nonulcer-mucosal lesions were significantly unrelated to H. pylori infection. Patients with concurrent cirrhosis with peptic ulcer were found to be negative for H. pylori infection. Conclusion Peptic ulcer is the leading cause of UGIB in Thailand. However, its incidence is declining. Patients who presented to hospital with UGIB were older, compared with those a decade ago. H. pylori infection plays an important role in UGIB and its incidence was stable during the past 5 years.

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