scholarly journals POTENSI EKSTRAK TUMBUHAN ANDALIMAN (Zanthoxylum acanthopodium DC.) SEBAGAI ANTIBAKTERI Staphylococcus aureus

Alotrop ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 133-139
Author(s):  
Oma Sepriani ◽  
Nurhamidah Nirhamidah ◽  
Dewi Handayani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Activity Test ◽  
North Sumatra

The aims of this study is to determine the secondary metabolite compounds in Roots, stem bark, leaves and fruit of Andaliman (Zanthoxylum acanthopodium DC), and determine the most potent part of Andaliman plant extract in inhibiting the growth of Staphylococcus aureus bacteria. Andaliman plant taken in Simarpingan, Sipaholon village sub district of North Sumatra. Part of the Andaliman plantar are cleaned, cut into small pieces and then dried and mashed, samples that have been finely phytochemically tested and extracted. Testing of secondary metabolite compound by doing phytochemical screening of roots, stem bark, leaves and fruit of Andaliman, alkaloid test using meyers reagent, terpenoid test and steroid using TLC plate with eluent n-hexana : ethyl acetate with ratio 6:4, flavonoid test using soluton HCl and Mg band, saponin test using soap test, tannin test using ferro (III) chloride solution. Test of antibacterial activity using disc diffusion method. Phytochemical results showed that all parts of the plant andaliman contain secondary metabolite compounds namely alkaloids, flavonoids, terpenoids, tannins and also saponins. The result of antibacterial activity test of leaf extract of leaf and leaf which has the most potential as antibacterial of S.aureus is bark. Because it able to inhibit S.aureus growth with 11 mm inhibition zone at concentration 5x104 ppm, which is included in the strong category. In addition to skin of andaliman leaf stem is also potential as an antibacterial with a 10 mm inhibition zone at concentration 5x104 ppm, which is included in the medium category.

scholarly journals AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli

2019 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
Yuni Widyastuti ◽  
Nia Yuliani ◽  
I.G.A. Manik Widhyastini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Aloe Vera ◽  
Bacterial Count ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Escherichia Coli Bacteria

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, dan   Escherichia coli,   Infusa.

scholarly journals Isolation, Characterization and Antibacterial Activity of Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) from Kenyan Ganoderma lucidum

2020 ◽  
pp. 48-57
Author(s):  
Ebrahim Sande ◽  
Danstone Lilechi Baraza ◽  
Selline Ooko ◽  
Peter Kuloba Nyongesa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Ganoderma Lucidum ◽  
Methicillin Resistance ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Significant Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Aims: To determine the chemical composition and antibacterial activity of Kenyan Ganoderma lucidum. Study Design: Structural determination of the isolated compound was done using spectral evidences and in comparison with literature. The antibacterial properties of the compound was done using disc diffusion method. Place and Duration of Study: Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, between January and November, 2019. Methodology: Sequential extraction of dried samples of Kenyan G. lucidum were done using solvents hexane, ethyl acetate and methanol. Chromatographic separation of hexane extract of Ganoderma lucidum was done using spectroscopic data. The compound was assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin–Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Streptococcus pyogenes. Standard antibiotic namely; ampicillin was used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial activity. Results: From hexane extract of Ganoderma lucidum, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated. Ethylacetate and methanol extracts produced a mixture of complex compounds. Ergosta-5,7,22-triene-3β,14α-diol (22Z) exhibited significant activity against Methicillin-Resistance Staphylococcus aureus (MRSA) (p=0.022) and Streptococcus pyogenes (p = 0.05). The most sensitive microbe was Streptococcus pyogenes. Conclusion: One major compound, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated, characterized and antibacterial activity determined.

scholarly journals Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Western Ghats ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

scholarly journals ISOLATION OF SECONDARY METABOLITE COMPOUNDS AND ANTIBACTERIAL ACTIVITIES TESTS FROM HEXANE EXTRACT OF STEM BARK MELOCHIA UMBELLATA (HOUTT) STAPF VAR. DEGRABRATA K

2018 ◽  
Vol 11 (8) ◽  
pp. 457 ◽  
Author(s):  
Usman Usman ◽  
Muh Amir M ◽  
Nunuk Hariani Soekamto ◽  
Ahyar Ahmad ◽  
Maulidiyah Maulidiyah
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Bark Extract ◽  
Separation And Purification ◽  
Inhibitory Zone

Objectives: This research aims to determine the content of secondary metabolite compounds and antibacterial activity of stem bark extract Melochia umbellata (Houtt) Stapf var. degrabrata.Methods: M. umbellata stem bark was extracted by maceration using methanol solvent. Separation and purification were done by partitioning, fractionation with chromatography, and recrystallization. Antibacterial activity test of hexane extract and third isolate from the bark of M. umbellata was done by agar diffusion method against bacterium Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.Results: Phytochemical test showed that the hexane extracts of bark M. umbellata containing alkaloids and triterpenoids. Isolate D is a triterpenoid group compound, while FKa and FKb are steroid group compound. The hexane extract had the highest antibacterial activity against B. subtilis bacteria with inhibitory zone diameter 12.0 mm. Isolate D has a weak inhibitory effect on all test bacteria. The highest diameters inhibition zone of isolated FKa compound against B. subtilis and S. aureus bacteria was 18.0 mm and 13.0 mm, respectively, whereas, the highest diameter inhibition of zone FKb compound against B. subtilis bacteria with inhibitory zone was 12.0 mm.Conclusion: The FKa compound from the bark of M. umbellata has the potential to be antibacterial because the compound is able to inhibit bacterial growth with ˃14 mm obstacle zone, especially against B. subtilis bacteria.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK METANOL Acalypha hispida TERHADAP BAKTERI Shigella flexneri DAN Bacillus cereus IHB B 379

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Elvi Rusmiyanto P. Wardoyo ◽  
Devinda Ekarizky Diputri ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Methanol Extract ◽  
Shigella Flexneri ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Extract Concentration ◽  
Activity Test ◽  
Antibacterial Potential

AbstractAcalpha hispida flower commonly used as traditional medicine. The antibacterial potential of the A. hispida flower can be used to treat diarrhea and cancer. The purpose of this study was to determine the antibacterial activity and effective concentration of methanol extract of A. hispida flower against to S. flexneri and B. cereus. Sample used was flower of A. hispida from Kota Pontianak. The solvent used is metahnol. Antibacterial activity test using the disc diffusion method (Kirby-Bauer) with parameters based on grading levels  extract concentration in S. flexneri was 1.2;  1.3;  1.4;  1.5;  and 1.6 g/ml and B. cereus for 0.75;  0.80;  0.85;  0.90;  and 0.95 g/mL, 10% DMSO and ciprofloaxin as a comparison. the results of the research showed that the concentration of methanol extract of A. hispida flower which can effectively inhibit the growth of S. flexneri and B. cereus bacteria at 1.2 g / mL and 0.75 g / mL. The methanol extract of A. hispida flower contains compounds of alkaloids, flavonoids, tannins, phenols, steroids and terpenoids.Keywords : Acalypha hispida, Antibacterial, Shigella flexneri, Bacillus cereus AbstrakAcalypha hispida merupakan tanaman obat trardisional yang sudah dimanfaatkan masyarakat secara turun temurun untuk mengobati diare dan kanker. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri dan konsentrasi efektif ekstrak metanol bunga A. hispida terhadap bakteri Shigella flexneri dan Bacillus cereus. Sampel penelitian yang digunakan adalah bunga A. hispida yang diperoleh dari Kota Pontianak. Pelarut yang digunakan adalah methanol. Uji aktivitas antibakteri menggunakan metode difusi cakram (Kirby-Bauer) dengan perlakuan 5 taraf konsentrasi ekstrak pada bakteri S. flexneri sebesar 1,2; 1,3; 1,4; 1,5; dan 1,6 g/ml serta pada bakteri B. cereus IHB B 379 sebesar 0,75; 0,80; 0,85; 0,90; dan 0,95 g/mL, DMSO 10% serta sebagai pembanding digunakan ciprofloaxin. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak methanol bunga A. hispida yang secara efektif dapat menghambat pertumbuhan bakteri S. flexneri dan B. cereus sebesar 1,2 g/mL dan 0,75 g/mL. Ekstrak methanol bunga A. hispida mengandung senyawa golongan alkaloid, flavonoid, tannin, fenol, steroid dan terpenoid.Kata kunci: Acalypha hispida, antibakteri, Shigella flexneri, Bacillus cereus

scholarly journals Uji Aktivitas Antimikroba Ekstrak Jamur Lingzhi (Ganoderma lucidum) Menggunakan Pelarut Etanol 96% Terhadap Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 41-44
Author(s):  
Prasetyo Handrianto
Keyword(s):  
Staphylococcus Aureus ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Traditional Medicines ◽  
Paper Disc

ABSTRAKStaphylococcus aureus merupakan bakteri yang relatif sering dijumpai pada manusia. Beberapa infeksi yang disebabkan oleh Staphylococcus aureus antara lain, bisul, infeksi luka dan pneumonia. Salah satu obattradisional yang dapat digunakan untuk mengobati infeksi yang disebabkan oleh Staphylococcus aureus adalah jamur lingzhi (Ganoderma lucidum). Penelitian dilakukan dengan 5 konsentrasi ekstrak etanol jamur lingzhi, yaitu kontrol negatif (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml dan 100 µg/ml dan dilakukan 6 kali pengulangan. Metode yang digunakan dalam uji aktivitas antimikroba yaitu metode difusi kertas cakram. Data zona hambat dianalisis dengan uji Anova One-Way. Hasil penelitian menunjukkan bahwa ekstrak etanol jamurlingzhi (Ganoderma lucidum) dengan konsentrasi 100 µg/ml memiliki rata-rata daya hambat tertinggi yaitu sebesar 18,0 mm (sangat aktif). Dapat disimpulkan bahwa semakin besar konsentrasi ekstrak jamur lingzhi (Ganoderma lucidum), maka akan berpengaruh besar pula terhadap zona hambat bakteri Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.ABSTRACTStaphylococcus aureus is a bacteria that can cause infections , such as boils , wound infections and pneumonia. One of the traditional medicines that can be used to treat these infections are fungal lingzhi (Ganodermalucidum). The study was conducted with five concentrations of negative control (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml, and 100 µg/ml with six repeats. The method used in the test of antimicrobial activity is paper disc diffusion method. Data of inhibition zone were analyzed by Anova-One Way test. The results showed that the ethanol extract of lingzhi mushroom (Ganoderma lucidum) at concentration 100 µg/ml has highest average of inhibitory regions as big as 18,0 mm. It can be concluded that the greater the concentration of extract of lingzhi mushroom (Ganoderma lucidum) , it will affect the greater the inhibition zone against Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.

scholarly journals Formulation and Antibacterial Activity of Liquid Soap Containing Ketapang (Terminalia catappa L.) Leaves Extract

2021 ◽  
Vol 4 (1) ◽  
pp. 43-50
Author(s):  
Asiska Permata Dewi ◽  
Dini Mardhiyani
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Foam Stability ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Liquid Soap ◽  
Disc Diffusion Method ◽  
Inhibition Zone Diameter ◽  
Terminalia Catappa

Ketapang (Terminalia catappa L.) is traditionally used by the community to treat infections of the skin caused by bacteria or fungi. In this study, T. catappa leaves extract was added to the liquid soap formula as an antibacterial. The purpose of this study was to determine the secondary metabolite compounds contained in T. catappa leaves extract, physical evaluation of the preparation, and antibacterial activity of liquid soap. Liquid soap formula is made with various concentrations of T. catappa leaves extract F0 (0%), F1 (1%), F2 (2%), and F3 (3%). The resulting soap was evaluated for organoleptic, pH, high foam, homogeneity, irritation, and its activity against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli using the disc diffusion method. The results showed that the T. catappa leaves extract contained flavonoids, tannins, saponins, and triterpenoids. The liquid soap formula F0 is clear, while F1, F2, and F3 have the characteristics of brown-dark brown, homogeneous, pH between 4.6-5.2, foam stability between 67-72%, which is not significantly different and stable after five minutes of testing, and it does not irritate the skin. Terminalia catappa leaves extracts liquid soap has antibacterial activity at a concentration of 1%, 2%, and 3%, with the largest inhibition zone diameter produced by S. aureus.

scholarly journals Aktivitas Antibakteri Ekstrak Metanol dari Kulit Batang Kelor (Moringa oleifera Lam.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Nurul Cholifah ◽  
Ahmad Ridhay ◽  
Pasjan Satrimafitrah ◽  
Ruslan ◽  
Hardi Ys
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Moringa Oleifera Lam

Antibacterial activity of Moringa oleifera Lam. stem bark has been tested against Staphylococcus aureus and Escherichia coli. The extraction of Moringa oleifera Lam. stem bark was used maceration method with methanol solvent and has obtained extract yield of 6.1%. The antibacterial activity test of Moringa oleifera stem bark extracts used a well diffusion method. The concentration of Moringa oleifera stem bark extract was varied to four concentrations of 1% 2% 3% 4% (w/v). The inhibition zone of methanol extract of Moringa oleifera stem bark against Staphylococcus aureus at extract concentrations of 1%, 2%, 3%, and 4% was 10.08 mm, 11.8 mm, 15.00 mm, and 17.02 mm, respectively. The methanol extract of Moringa oleifera stem bark at concentrations of 1%, 2%, 3%, and 4% could also inhibition of Escherichia coli growth with inhibition zone of 14.01 mm, 16.50 mm, 17.09 mm, and 17.10 mm, respectively. Keywords: Moringa Oleifera Lam., Antibacterial, Staphylococcus aureus, Escherichia coli

scholarly journals The mechanism of antibacterial activity of corylifolinin against three clinical bacteria from Psoralen corylifolia L

2018 ◽  
Vol 16 (1) ◽  
pp. 882-889 ◽  
Author(s):  
Nan He ◽  
Juan Zhou ◽  
Miyun Hu ◽  
Changyang Ma ◽  
Wenyi Kang
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Extracellular Protein ◽  
Diffusion Method ◽  
Antibacterial Mechanism ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Scanning Electron

AbstractEffective antibacterial activity against Grampositive bacteria isolated in clinical strains was exhibited when corylifolinin was tested using the Disc diffusion method (K-B method). Minimum inhibitory concentration (MIC) of corylifolinin against Staphylococcus aureus (SA), Methcillin-resistant Staphylococcus aureus (MRSA) and extended spectrum β-lactamases Staphylococcus aureus (ESBLs-SA) were 0.078, 0.156 and 0.078 mg/mL, respectively. The minimum bactericide concentrations (MBCs) were 0.156, 0.156 and 0.078 mg/mL respectively. Scanning electron microscopy (SEM), alkaline phosphatase (AKP) and bacterial extracellular protein leakage were used to investigate the antibacterial mechanism of corylifolinin. After adding corylifolinin at the MBC level, there were obvious changes to the Staphylococcus aureus of bacteria cells. Both MIC level and MBC levels of corylifolinin led to the leakage of AKP and bacterial extracellular protein.

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