Antibacterial activity of di-2-ethylaniline phosphate screened by paper disc diffusion method

Meat is one of the food products that can easily be damaged, ( therefore it is necessary to preserve the process). therefore it needs to be done the process of preservation. In this study we studied the anti-bacterial activity of Alpinia luteocarpa extract against fresh meat rot bacteria, with paper disc diffusion method which is the standard recommended by the Clinical and Laboratory Standard Institute (CLSI,2012). The results of antibacterial activity test showed extract and fraction of A. luteocarpa able to inhibit bacterial growth B.cereus ATCC 21772 and. K. pneumonia ATTC 13773. The bacteriostatic properties of the extract and fraction of A. luteocarpa against B. cereus ATCC 21772 and K. pneumonia ATTC 13773 were better than the positive control of chlorhexidine antibiotics (500 μg / mL) with inhibition zones 9-11 mm and 7-9 mm.

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AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DADAP SEREP (Erythrina lithosperma Miq.) TERHADAP PERTUMBUHAN Escherichia coli

Pharmacoscript ◽

10.36423/pharmacoscript.v1i1.105 ◽

2019 ◽

Vol 1 (1) ◽

Author(s):

Asep Abdul Rahman ◽

Ridwan Firmansyah ◽

Lulu Setyabudi

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Abdominal Pain ◽

Leaf Extract ◽

Ethanol Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Activity Test ◽

Paper Disc

Dadap Serep (Erythrina lithosperma Miq.) is one of the plants containing secondary metabolites in the form of alkaloids, flavonoids and tannins. Empirically dadap serep can be used to treat fever, abdominal pain, breast milk, prevent miscarriage, inflammation and cough. The aim of this research is to know the activity of ethanol extract in dadap serep (Erythrina lithosperma Miq.) in inhibiting growth of Escherichia coli ATCC 8939. The dadap serep leaves were extracted by maceration method using 96% ethanol solvent. The leaf thickness extract of dadap serep with concentration of 20%, 40%, 60% and 80% tested its antibacterial activity against growth of Escherichia coli ATCC 8939 using paper disc diffusion method. The result of concentration of leaf extract of dadap serep, obtained rendemen as much as 23,53%. Then the result of antibacterial activity test showed that leaf extract of dadap serep with concentration of 20% had no inhibition to growth of Escherichia coli ATCC 8939, while at concentration extract 40%, 60%, and 80% had inhibitory to growth of Escherichia coli ATCC 8939 with category weak resistor response.Keywords: Dadap serep (Erythrina lithosperma Miq.), Antibacterial activity, Escherichia coli ATCC 8939

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Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

BioMed Research International ◽

10.1155/2014/521287 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 14

Author(s):

Somayeh Razmavar ◽

Mahmood Ameen Abdulla ◽

Salmah Binti Ismail ◽

Pouya Hassandarvish

Keyword(s):

Antibacterial Activity ◽

Ethanol Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Phytochemical Screening ◽

Bioactive Constituents ◽

Disc Diffusion Method ◽

Leaf Extracts ◽

Baeckea Frutescens ◽

High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

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EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

10.36106/paripex/2805137 ◽

2021 ◽

pp. 77-79

Author(s):

Janardhan Namdeo Nehul

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Methanol Extract ◽

Axenic Culture ◽

Soil Samples ◽

Diffusion Method ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Disc Diffusion Method ◽

Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

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Synthesis of a New Conjugates of Imidazole wih Beta Lactam Moiety and Evaluation of its Expanded Antibacterial Activity

Oriental Journal Of Chemistry ◽

10.13005/ojc/340535 ◽

2018 ◽

Vol 34 (5) ◽

pp. 2495-2501 ◽

Cited By ~ 1

Author(s):

Sarmd D. Noori ◽

Mazin N. Mousa ◽

Shaker A. N. Al-Jadaan

Keyword(s):

Antibacterial Activity ◽

Chemical Structure ◽

Bacterial Species ◽

Anaerobic Bacteria ◽

Diffusion Method ◽

Disc Diffusion ◽

Beta Lactam ◽

Disc Diffusion Method ◽

E Coli ◽

Ft Ir

Five compounds containing (2,4,5-triphenyl triphenyl-1H-) and azetidinone (beta-lactam) moiety were synthesized. The physical data and yield of synthesized compounds were recorded, the chemical structure of prepared compounds were characterized using FT-IR, 1H-NMR and elemental analysis. The antibacterial activity was evaluated using disc diffusion method that involve tow Gram positive (staph. aureus, E. Faecalis), two Gram negative (E. coli and ‎K. pneumoniae), and one anaerobic bacteria (streptococcus. Pyogen). Different concentration of the prepared compounds has been used, and the obtained result were compared with standard (ceftazidime). Compound (5c) showed the best antibacterial activity against all bacterial species while 5a and 5e does not. Other compounds showed activity against some species.

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Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

Natural Product Communications ◽

10.1177/1934578x0700200517 ◽

2007 ◽

Vol 2 (5) ◽

pp. 1934578X0700200

Author(s):

Fadwa El Hanbali ◽

Ahmed El Hakmaoui ◽

Fouad Mellouki ◽

Lahoussine El Rhaffari ◽

Mohamed Akssira

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

Jurnal Teknologi ◽

10.11113/jt.v77.6747 ◽

2015 ◽

Vol 77 (25) ◽

Cited By ~ 1

Author(s):

Nur Azfa Shuib ◽

Anwar Iqbal ◽

Fatimatul Akmal Sulaiman ◽

Izzatie Razak ◽

Deny Susanti

Keyword(s):

Antibacterial Activity ◽

Phenolic Content ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Diffusion Method ◽

Total Phenolic ◽

Disc Diffusion ◽

Flavonoid Content ◽

Disc Diffusion Method ◽

Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

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A Study on Antibacterial Activity of Silver Nanoparticles

TAJ Journal of Teachers Association ◽

10.3329/taj.v29i2.39106 ◽

2018 ◽

Vol 29 (2) ◽

pp. 37-41

Author(s):

M Iqbal Hossain ◽

M Anwar Habib ◽

Nazimuddin Ahmed

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Antibacterial Effect ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Reference Drug ◽

Gram Negative ◽

Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

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Synthesis and in vitro antibacterial activity of Cephradine Benzoate

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v10i3.8356 ◽

1970 ◽

Vol 10 (3) ◽

pp. 148-151

Author(s):

M Ahmad ◽

M Rahman ◽

P Kumar Paul

Keyword(s):

Antibacterial Activity ◽

Salmonella Typhi ◽

Shigella Dysenteriae ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Nmr Studies ◽

Shigella Boydii ◽

Group B

Aims & Methods: The present study was undertaken to compare the antibacterial activity of a cephradine derivative with that of the parent antibiotic cephradine. Cephradine was converted to its benzoyl derivative by Schotten-Baumann method for the first time. Disc diffusion method was employed to find out the antibacterial activity against EPEC, ETEC, E. Agg, Salmonella typhi, Salmonella group B, Shigella boydii, Shigella dysenteriae 1, Shigella dysenteriae 2, Shigella flexinariae and Shigella sonnei. Melting point, TLC, HPLC, UV, FTIR and <sup>1</sup>H NMR studies were carried out to check the purity and confirm that the derivative was cephradine benzoate. Results: The benzoyl derivative showed promising activity against tested bacteria. The results obtained from the study demonstrate that the benzoyl derivative could be a potential antibacterial agent. Key words: Cephradine benzoate, antibacterial activity, disc diffusion method. DOI: http://dx.doi.org/ 10.3329/bjms.v10i3.8356 BJMS 2011; 10(3): 148-151

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Studies of Antimicrobial Activity of 2',4',5'- and 2',3',4'-Trimethoxy Flavanones

Journal of Scientific Research ◽

10.3329/jsr.v8i2.26622 ◽

2016 ◽

Vol 8 (2) ◽

pp. 209-216

Author(s):

A. Rahim ◽

R. Ali ◽

A. Islam

Keyword(s):

Pathogenic Bacteria ◽

Sclerotium Rolfsii ◽

Antibacterial Activities ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Filter Paper Disc ◽

Antibacterial And Antifungal ◽

Paper Disc

2',4',5'- and 2',3',4'-trimethoxy flavanones have been synthesized starting with 2-hydroxyacetophone and substituted aldehyde. Antibacterial activities of the flavanones have been tested along with their corresponding chalcones against two human pathogenic bacteria (Streptococcus-b-haemolyticus and Klebsiella sp. (G-)). Antifungal activities of the flavanones have also been investigated against two plants pathogenic mold fungi (Rhizactonia solani Sclerotium rolfsii). The structures of the synthesized compounds have been characterized with the help of UV, IR and 1H NMR and 13C-NMR spectra. The antibacterial and antifungal screening were performed in vitro by the filter paper disc diffusion method and poisoned food technique. The flavanones showed antibacterial activity while no activity was observed to their corresponding chalcones against the tested bacteria. On the other hand, chalcones and their corresponding flavanones both showed fungicidal activities.

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