scholarly journals Effect of Roselle Hibiscus sabdariffah extract on some pathogenic microorganism

2011 ◽  
Vol 5 (3) ◽  
pp. 24-31
Author(s):  
Lamees M. R. Abbas ◽  
Laith A. Yaaqoub ◽  
Sabah Mahdie
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Plant Extract ◽  
Minimum Bactericidal Concentration ◽  
Salmonella Typhi ◽  
Inhibition Activity ◽  
Pathogenic Microorganism ◽  
Proteus Vulgaris ◽  
Inhibition Concentration ◽  
Mic Value

The inhibition activity for the aqueous and alcoholic extracts of Hibiscus sabdariffah being studied by the wells method on some microorganisms such as Staphelococcus aureous, Streptococcus epidermidis, Serratia marcesens, Proteus vulgaris, Enterococcus fecalus, Klebsiella sp, Candida albicans, Asparaglas niger. Escherichia coli, Salmonella typhi, The results also revealed that, the aqueous and alcoholic extracts of Hibiscus sabdariffah had inhibition effects for all microorganism, the cold aqueous showed the higher inhibition than the hot and alcoholic extracts of the plant on Serratia marcesens, Escherichia coli, Enterococcus fecalus at concentration (25,50,75,100)%, The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the plant extract. The results showed that the (MIC) value of all plant extract were (10, 2.5) % on Serratia marcesens, Escherichia coli respectively, were the value of (MBC) was (1.25, 5) % for the same bacteria

scholarly journals Quantitative Phytochemical Analysis and Antimicrobial Potential of the Ethanol and Aqueous Extracts of the Leaf, Stem and Root of Chromolaena odorata (Asteraceae)

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Ugwoke C E C ◽  
Orji J. ◽  
Anze S P G ◽  
Ilodibia C V
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Infectious Diseases ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Chromolaena Odorata

Background: Plants contain secondary metabolites or phytochemicals, which when consumed by humans give therapeutic effect. This study therefore analyzed the phytochemical composition of Chromolaena odorata so as to give an idea of its possible pharmacological potentials. An antimicrobial assay was also carried out to verify claims on its use in the treatment of infectious diseases. Methods: The experimental procedure involved collection of the leaf, stem and root of the plant from the wild, authenticating the samples and drying under shade to facilitate pulverization. Preliminary qualitative and quantitative phytochemical analyses were done using standard methods to reveal the presence and percentage composition of basic phytochemicals. The powder was also macerated in ethanol and water to produce ethanol and aqueous crude extracts that were reconstituted in normal saline to concentrations (mg/ml) of 150, 100, 75 and 50. Clinical isolates of Staphylococcus aureus, Salmonella typhi, Escherichia coli, Aspergillus niger and Candida albicans were screened for sensitivity to the extracts using the agar well diffusion method. Results: The plant parts contain alkaloids, tannins, flavonoids, saponins, terpenoids, phenols, cardiac glycosides, and sterols at varying concentrations. The leaf however had highest concentration of almost all phytochemicals present. The antimicrobial activity of the plant was concentrationdependent in all parts of the plant and both extracts. Aspergillus niger and Candida albicans were most susceptible while Salmonella typhi and Escherichia coli were least susceptible with an average Inhibition Zone Diameter (IZD) of about 8 – 10 mm. Conclusion: The substantial quantity of the basic phytochemicals in Chromolaena odorata could render it a utility plant in therapeutic use. Due to the profound antimicrobial effect as revealed by the IZDs, the plant could be classified as a broad spectrum antimicrobial agent. Thus, the claim on its potency in treatment of infectious diseases by traditional medical practitioners could be said to be justified.

scholarly journals Isolation of Bifidobacterium from Infant’s Feces and Its Antimicrobial Activity

2020 ◽  
Vol 2 ◽  
pp. 00002
Author(s):  
Dyah Fitri Kusharyati ◽  
Pancrasia Maria Hendrati ◽  
Dini Ryandini ◽  
Tsani Abu Manshur ◽  
Meilany Ariati Dewi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Inhibition Activity ◽  
Antimicrobial Substance ◽  
Inhibitory Zone ◽  
E Coli

<p class="Abstract"><i>Bifidobacterium</i> is a group of Lactic Acid Bacteria (LAB) that commonly found in the gastrointestinal tract and vagina. LAB has many health benefits, such as produce an antimicrobial substance against a pathogen. This research aims to isolate <i>Bifidobacterium</i> from an infant’s feces and know its antimicrobial activity against <i>Escherichia coli</i> and <i>Candida albicans.</i> A total of 5 isolates <i>Bifidobacterium</i> spp. were isolated from the sample. <span lang="EN">The largest inhibitory activity against <i>E. coli</i> was shown by isolate Bb3F, with the inhibitory zone of 10.80 mm. While the largest inhibition activity against <i>C. albicans</i> was shown by isolate Bb1B and Bb3F with the inhibitory zone of 9.70 mm.</span><o:p></o:p></p>

Composition and Antimicrobial Activity of Achillea Distans Essential Oil

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Angel Konakchiev ◽  
Milka Todorova ◽  
Bozhanka Mikhova ◽  
Antonina Vitkova ◽  
Hristo Najdenski
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Essential Oil ◽  
Salmonella Typhimurium ◽  
Moderate Activity ◽  
Proteus Vulgaris ◽  
Oil Composition ◽  
Weak Activity

The essential oil of Achillea distans W. et K. flower heads was analyzed by GC and GC-MS. Altogether 43 components in concentrations more than 0.1% were identified representing 93.5% of the oil composition. The main constituents were 1,8-cineole (16.8%), trans-thujone (9.8%), sabinene (8.2%), borneol (7.5%), β-pinene (6.5%), and camphor (5.8%). The oil showed moderate activity against Staphylococcus aureus and Candida albicans, and weak activity against Salmonella typhimurium, Proteus vulgaris, and Escherichia coli.

Activité antimicrobienne de l’huile essentielle de galles du Pistachier de l’Atlas (Pistacia atlantica Desf.) en Algérie

2019 ◽  
Author(s):  
I. Sifi ◽  
M. Yousfi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Salmonella Typhi ◽  
Huiles Essentielles ◽  
Activité Antimicrobienne ◽  
Pistacia Atlantica

L’activité antimicrobienne des huiles essentielles de galles de Pistacia atlantica récoltées dans trois régions (Aïn-Oussara, Laghouat et Kheneg) a été évaluée sur sept souches pathogènes (Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, MRSA+, Salmonella typhi et Candida albicans), en utilisant les méthodes de diffusion sur gélose, de microdilution et celle de microatmosphère (seulement pour Candida albicans). Les huiles essentielles ont révélé un effet antimicrobien remarquable contre les micro-organismes testés, avec des valeurs de CMI et de CMB variant de 0,13 à 7,99 μl/ml et de 0,25 à 7,99 μl/ml, respectivement. Une activité importante a été observée pour les échantillons de la station Kheneg contre l’espèce Candida albicans avec une zone d’inhibition de 68 mm (50 μl). Ces résultats suggèrent que les huiles essentielles testées pour leur activité antimicrobienne peuvent être classées comme bactéricides.

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)

2017 ◽  
Vol 9 (3) ◽  
pp. 75
Author(s):  
Rajanikanth Garapati ◽  
N. Ramesh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Proteus Vulgaris ◽  
Methanol Extracts ◽  
Tract Infections

Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.

scholarly journals Antimicrobial activity of aqueous extracts of pomegranate, sumac, sage, anise, hand bull tongue, thyme, cloves, lemon and mint against some food-borne pathogens

2010 ◽  
Vol 34 (2) ◽  
pp. 85-94
Author(s):  
Maha A. Mahmood
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
The Other ◽  
Aqueous Extracts ◽  
Food Borne Pathogens ◽  
Food Borne

The increasing rate of resistance development for commonly used antibiotics have led to search for newer, more effective, affordable and easily available medicine. Medicinal plants have revived as a consequence of current problems associated with the use of antibiotics. Aqueous extracts of nine plants (pomegranate, sumac, sage, anise, hand bull tongue, thyme, cloves, lemon and mint) were qualitatively and quantitatively examined against twenty microbial isolates, mostly food borne including pathogens. (E.coli, Salmonella typhi and Candida albicans) antimicrobial screening was done by agar diffusion (well diffusion) and minimal inhibitory concentration (MIC) methods. Among the screened plants, cloves were the most inhibitor against isolates of Escherichia coli, Salmonella typhi and Candida albicans followed by the inhibitory effect of sumac against (E.coli, Salmonella typhi and Candida albicans). On the other hand, pomegranate, sage and lemon showed varied inhibitory effect against the tested food borne isolates, whereas the extracts of anise, hand bull tongue, thyme and mint showed no antimicrobial activities against most of the tested isolates. Regarding the inhibition zone and MIC results, the present study certified that Candida albicans was the most sensitive pathogen as compared with the other food borne pathogens tested (Escherichia coli and Salmonella typhi).

scholarly journals In-Vitro Evaluation of Antimicrobial Activities of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans Nosodes

Homeopathy ◽  
2021 ◽  
Author(s):  
Renuka Munshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
E Coli ◽  
Inhibition Of Growth

Abstract Background This study presents the results of the minimum inhibitory concentration (MIC) assay of a series of nosodes: namely Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans. Each was tested against its corresponding infection as well as cross infections. Methods In-vitro efficacy of polyvalent nosodes was tested using the MIC assay technique. The nosodes, namely C. albicans polyvalent nosode (35c, 100c), N. gonorrhoeae (35c), K. pneumoniae (35c, 100c), E. coli polyvalent nosode (35c, 100c) and Salmonella typhi polyvalent nosode (30c, 100c), were tested along with positive and negative controls. Nosodes were studied in different potencies and at 1:1 dilution. Results C. albicans polyvalent nosode 35c, 100c, N. gonorrhoeae 35c, and positive control amphotericin B showed inhibition of the growth of C. albicans species. K. pneumoniae 35c, E. coli polyvalent nosode 100c, and meropenem (positive control) showed inhibition of the growth of K. pneumoniae; this effect was not seen with ceftriaxone, ofloxacin and amoxicillin antibiotics. E. coli polyvalent nosode 30c in 10% alcohol (direct and dilution 1:1) and the positive controls ciprofloxacin, ofloxacin, and amoxicillin showed inhibition of the growth of E. coli. The S. typhi polyvalent nosode 30c in 10% alcohol showed inhibition of growth of S. typhi. Conclusion This study reveals that the tested nosodes exhibited antibacterial potential against the corresponding micro-organisms and against other selected organisms studied using this assay.

scholarly journals Bifidobacterium from infant stool: the diversity and potential screening

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
DYAH FITRI KUSHARYATI ◽  
HENDRO PRAMONO ◽  
DINI RYANDINI ◽  
TSANI ABU MANSHUR ◽  
MEILANY ARIATI DEWI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Inhibition Activity ◽  
Exopolysaccharide Production ◽  
Healthy Infants ◽  
Stool Samples

Abstract. Kusharyati DF, Pramono H, Ryandini D, Manshur TA, Dewi MA, Khatimah K, Rovik A. 2020. Bifidobacterium from infant stool: the diversity and potential screening. Biodiversitas 21: 2506-2513. Bifidobacteria spp. are a group of Lactic Acid Bacteria commonly found in the gastrointestinal tract of adults and infants. LAB are known as probiotics and have many health benefits. This research aimed to isolate Bifidobacteria from infant stool, identify, explore their diversity, and screen their potential as probiotics. Stool samples were collected from 3 healthy infants in Banyumas Regency. The potential screening included lysozyme resistance, antimicrobial activity, and exopolysaccharide production. A total of 7 Bifidobacterium species were isolated from infant stool: B. catenulatum, B. minimum, B. indicum, B. dentium, B. asteroides, B. galicum, and B. coerinum. B. indicum isolates (Bb3F and Bb1B) had the greatest inhibition activity against Escherichia coli and Candida albicans with 10.80 and 9.70 mm, respectively. Bifidobacteria isolates were resistant to lysozyme from egg whites up to 200 µg.mL-1. B. catenulatum Bb1A isolate had the highest yield of exopolysaccharide production with 74 mg.L-1. Among them, three Bifidobacterium strains (Bb1B, Bb2A, and Bb2E) were considered potentially as probiotics.

scholarly journals Evaluation of Antimicrobial Effects of Four Selected Marine Macroalgae from Iskenderun Bay

2021 ◽  
Vol 9 (12) ◽  
pp. 2253-2259
Author(s):  
Selin Sayın ◽  
Betül Aydın ◽  
Burcu Ak Çimen ◽  
Leyla Açık
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activities ◽  
Marine Macroalgae ◽  
Pathogenic Microorganisms ◽  
Proteus Vulgaris ◽  
Bacterial Strains ◽  
C Elegans ◽  
Iskenderun Bay ◽  
Mic Value

In the present study, the seaweeds belong to Phaeophyaceae (Cystoseira elegans Sauvageau 1912, Cystoseira amentacea (C.Agardh) Bory 1832, Padina crassa Yamada 1931) and Florideophyceae (Corallina elongata J.Ellis & Solander 1786) collected from nearby Iskenderun-Turkey of Mediterranean Sea were detected for their antimicrobial activities against seven bacterial strains (Escherichia coli ATCC 35218, Bacillus cereus NRRL B-371, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Salmonella typhimurium ATCC 14028, Proteus vulgaris RSKK 96029). The antimicrobial activities were expressed as minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). According to the results obtained from MICs and MBCs values of the extracts on pathogenic microorganisms were between 0.78 to 50 mg/mL. The lowest MICs and MBCs values were recorded for C. elegans extract against B. cereus with a MIC value of 0.78 mg/mL. These results suggest that secondary metabolites of brown and red algae are important sources that could be used as broad spectrum of biological and pharmaceutical activities.

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