scholarly journals Responses of different okra (Abelmoschus esculentus) cultivars to water deficit conditions

2021 ◽  
Vol 16 (1) ◽  
pp. 53-63
Author(s):  
Q Ayub ◽  
S M Khan ◽  
I Hussain ◽  
K Naveed ◽  
S Ali ◽  
...  

A pot experiment was conducted to evaluate the adverse effects of drought on different okra cultivars and to identify the most suitable okra cultivar for growing in drought conditions. Five okra cultivars namely Pusa Green, Clemson, Sabz Pari, Pusa Swani and Mehak Pari were subjected to three drought levels i.e., control (100% Field capacity),50% and 25% Field Capacity (FC). Physiological parameters like fresh and dry weight of plant and plant height were recorded along with biochemical attributes such as chlorophyll content (a, b, total) carotenoids, total protein, proline content, and Membrane stability index (MSI%). Results showed that drought significantly reduced all the studied parameters and at maximum drought (25% FC) lowest values of fresh weight (12.42g), dry weight (1.22g), plant height (7.86cm), chlorophyll a (9.02mg/g FW), chlorophyll b (18.69mg/g FW), total chlorophyll (27.71mg/g FW), carotenoids (11.80mg/g FW), total protein (2.73mg/g FW),whereas maximum Proline (21.36μg/g FW), and MSI (72%) were observed under the same drought. The results concerning responses of okra cultivars under drought conditions showed that maximum. Fresh weight (15.25g) and Dry weight (2.74g) was observed in Pusa green while maximum Plant height (13.77cm), Chlorophyll a (14.38mg/g FW), Chlorophyll b (24.41mg/g FW), Total Chlorophyll (38.80mg/g FW), Carotenoids (18.57mg/g FW), Total Protein (5.44mg/g FW), Proline (27.78μg/g FW), and MSI (56.33%) were produced by Sabz Pari. Hence it can be concluded that drought causes significant variation on physical and biochemical attributes of okra whereas Sabz Pari showed resistance towards the applied stress and produced better results.

Author(s):  
Qasim Ayub ◽  
Shah Masaud Khan ◽  
Abid Mahmood ◽  
Naveed Ul Haq ◽  
Shujaat Ali ◽  
...  

Horticultural crops especially vegetables are most vulnerable to drought due to their higher irrigation needs. Hence, this study was undertaken to evaluate the adverse effects of drought stress and the beneficial effects of salicylic acid (SA) on physiological and biochemical attributes of okra plants under drought stress. For this purpose, a pot experiment was laid out in Complete Randomized Design (CRD) design. Okra seeds were primed with four different SA treatments i.e., 0 (control), 1, 2 and 3 mM and sown in pots (Ø 20 cm). After 14 days of germination, the plants were subjected to two drought levels i.e., 25% and 50% field capacity (FC) and after 20 days of germination regular foliar sprays of SA at 7 days interval were performed with aforementioned SA levels. Physiological parameters like fresh weight, dry weight, and length of plants along with biochemical attributes like chlorophyll (‘a’, ‘b’ and total), total carotenoids, total protein and proline contents, and electrolyte leakage were recorded. Results revealed that drought stress (25% FC) significantly reduced all the studied parameters and resulted in the lowest values of fresh weight (5.04 g), dry weight (1.33 g), length of plants (11.68 cm), chlorophyll ‘a’ content (5.97 mg/g FW), chlorophyll ‘b’ content (8.86 mg/g FW), total chlorophyll (14.84 mg/g FW), total carotenoids (4.96 mg/g FW) and total protein (1.05 mg/g FW), except proline content (6.81 mg/g FW) and electrolyte leakage (77.31%) which was increased. Application of SA under drought stress reduced the harmful effects of drought and application of 2 mM SA produced the maximum fresh weight (8.60 g), dry weight (2.51 g), length of plants (16.23 cm), chlorophyll ‘b’ content (14.47 mg/g FW), total protein (3.73 mg/g FW) and proline content (5.11 mg/g FW); whereas application of 3 mM SA showed the highest values of chlorophyll ‘a’ (8.91 mg/g FW), total chlorophyll content (23.20 mg/g FW) and carotenoids (7.93 mg/g FW), and the lowest value for electrolyte leakage (62.00%).


2020 ◽  
Vol 5 (2) ◽  
pp. 144-150
Author(s):  
Sharmin Akter ◽  
Dilruba Yeasmin Jharna ◽  
Sujan Kanti Mali ◽  
Abu Sayeed

The present study was accomplished to find out the effects of salinity on germination, growth, physiological and biochemical processes of two different groundnuts (Arachis hypogaea L.), varieties viz., Dacca-1, and Zhinga groundnut. The experiments consisted of five salinity treatments viz., control (no salinity), 3, 6, 9, and 12dS/m during germination and vegetative stage of groundnut varieties. Results showed that germination percentage of both the groundnut varieties was significantly decreased with the increase of salt concentration. During the germination stage, maximum shoot and root length, shoot and root fresh weight and shoot and root dry weight of groundnut varieties were recorded from Zhinga groundnut variety at salt stress. The same result occurred during the vegetative stage of Dacca-1 variety while the Zhinga groundnut variety showed maximum parameters than Dacca-1. Fresh weight, as well as dry weight, of shoots and roots of both the groundnut varieties was also decreased with the increase of salt concentrations while leaf proline concentrations were increased among these varieties. Salinity caused significant (P<0.001) reduction in chlorophyll a, chlorophyll b, and total chlorophyll content in both the genotypes. Between two genotypes, Zhinga groundnut recorded higher chlorophyll a, chlorophyll b and total chlorophyll content than Dacca-1. From the studies, it is concluded that the groundnut genotype Zhinga was identified as the tolerant genotype to salt stress than Dacca-1. Overall results indicate that high salinity condition is not suitable for growing the groundnut.


2018 ◽  
Vol 5 (3) ◽  
Author(s):  
A F A Putra ◽  
N D Hanafi ◽  
Hamdan ◽  
M Tafsin ◽  
R E Mirwandhono

This study aims to determine effects of production of Brachiaria decumbens grass on different drought stress treatments. Research was conducted at the Greenhouse Faculty of Agriculture University of North Sumatera from Desember 2016 until April 2017. The design used was Completely Randomized Design (CRD), with three treatments and seven replications. The treatment consisted of three levels of drought stress which were control (100% field capacity=fc), medium drought stress (25% fc), and heavy drought stress (50% fc). The variable were fresh weight production, dry weight production and plant height. The results showed that drought stress treatment control (100% fc) significantly (P<0,05) affect fresh weight production, dry weight production, but not plant height. It is concluded that increasing water volume of field capacity increase production Brachiaria decumbens grass.


Author(s):  
Alam Khan Samim ◽  
B. S. Shivakumar ◽  
Ganapathi M.

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.


Author(s):  
Wahida Nia Elfiza ◽  
Abdi Dharma ◽  
Nasril Nasir

Abstrakb-karoten merupakan karotenoid yang bermanfaat sebagai antioksidan. Penelitian ini bertujuan menapis mikroalga yang berpotensi  mengandung karotenoid tinggi dan mempelajari pengaruh nitrogen dan fosfor terhadap produktivitas biomassa, kandungan pigmen fotosintesis dan b-karoten, pada mikroalga yang diisolasi dari perairan Danau Atas, Sumatra Barat. Penapisan mikroalga penghasil karotenoid dilakukan  dengan memberikan  paparan UV-A 326 nm terhadap kultur campuran mikroalga. Hasil penelitian menunjukkan bahwa 5 dari 18 spesies mampu bertahan pada proses penapisan. Pengaruh 9 jenis medium pertumbuhan dengan kriteria: tanpa NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, Bold Basalt Medium (BBM) normal (kontrol), tanpa KH2PO4, 3x KH2PO4, 5x KH2PO4,dan10x KH2PO4 terhadap mikroalga terpilih (Oocystis sp.) diamati. Hasil menunjukkan kandungan b-karoten tertinggi  ditemukan pada perlakuan 5x KH2PO4  yaitu sebesar 0,22 % dari berat kering mikroalga, dengan produktivitas biomassa 0,0015 g/mL/hari, serta kandungan klorofil a, klorofil b dan karotenoid total yaitu 7,15 µg/mL, 0,81 µg/mL dan 6,67 µg/mL. Berdasarkan penelitian ini dapat disimpulkan medium pertumbuhan dengan kandungan 5x KH2PO4 merupakan medium yang cocok bagi pertumbuhan Oocystis sp. untuk mendapatkan biomassa dengan kandungan b-karoten dan karotenoid tinggi tanpa harus menurunkan produktivitas biomassanya. Screening of Carotenoid Producing Microalgae and Study of the Effect of Nitrogen and Phosphorus Stress on the Production of b-Carotene in Microalgae Oocystis sp.Abstractb-carotene is a carotenoid that is useful as an antioxidant. Present study aims to screening high microalgae which contain carotenoids from water of Danau Atas lake (West Sumatra Province), and study the effect of nitrogen and phosphorus on biomass productivity, photosynthetic and b-carotene pigment content. Screening of carotenoid-producing microalgae was carried out by exposing UV-A 326 nm to mixed microalgae cultures. The results showed that 5 of the 18 species were able to survive in the screening process. The effect of growth medium, i.e. without NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, normal Bold Basalt Medium (BBM) (control), without KH2PO4, 3x KH2PO4, 5x KH2PO4, and 10x KH2PO4 on of selected microalgae (Oocystis sp.) was carried out. The result showed that the highest content of b-carotene of Oocystis sp. was obtained with 5x KH2PO4 which was 0.22% of the dry weight of biomass. Biomass productivity was 0.0015 g/mL/day and the chlorophyll a, chlorophyll b and total carotenoids contents were 7.15 µg/mL, 0.81 µg/mL and 6.67 µg/mL, respectively. Based on this research, 5x KH2PO4 can be concluded as a suitable medium for Oocystis sp. to obtained high b -carotene and carotenoid of Oocystis sp. without  reducing biomass productivity.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 982
Author(s):  
Arnold Szilágyi ◽  
László Radócz ◽  
Mária Takácsné Hájos ◽  
Csaba Juhász ◽  
Béla Kovács ◽  
...  

Woolly cupgrass (Eriochloa villosa (Thunb.) Kunth) is a new invasive weed in Hungary. This study was conducted to elucidate the effects of this weed on the biochemistry and growth of maize (Zea mays L. cv. Armagnac) under greenhouse conditions. Activities of the antioxidative enzymes (ascorbate peroxidase (APX), guaiacol peroxidase (POD), superoxide dismutase (SOD)), the contents of malondialdehyde (MDA), and protein were measured in the shoots and roots, whereas the content of the photosynthetic pigments was measured only in the shoots. The measured growth parameters included plant height, root length, root volume, root and shoot dry weight, and stem diameter. Results showed the allelopathic effects of woolly cupgrass on maize, with significant decreases in plant height, root length, root volume, and root dry weight. Woolly cupgrass infestation (WCI) induced significantly higher activities of APX and SOD in the shoots, whereas POD was only induced in the roots. The contents of chlorophyll-a, total chlorophyll (including relative chlorophyll), carotenoids, and root protein were substantially reduced by WCI, except for the leaf chlorophyll-b. The results suggest that high APX and SOD activities in the shoots could be involved in stabilizing the leaf chlorophyll-b, chlorophyll a/b, shoot protein, and shoot dry weight because all of these parameters were not inhibited when these two enzymes were induced. In contrast, high activity of POD in the roots is not effective in counteracting allelopathy. Therefore, it would be worthwhile to further investigate if an increase in the activities of APX and SOD in the shoots of WCI maize is responsible for stabilizing leaf chlorophyll-b, shoot protein, and shoot dry weight, which could contribute to improved maize yield under WCI.


2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.


2018 ◽  
Vol 33 (1) ◽  
pp. 34
Author(s):  
Samanhudi Samanhudi ◽  
Ahmad Yunus ◽  
Bambang Pujiasmanto

Potential development of medicinal plants in Karanganyar is very large, because this area is famous as a center of medicinal plants such as ginger, turmeric, kencur, and temulawak. In the development of medicinal plants, the approach of organic cultivation is one of the alternatives that can be done to achieve the business. Therefore the application of technology to society about organic farming on Biopharmaca Cluster of Karanganyar was conducted with the aim to apply organic cultivation techniques that have resulted from previous studies, so it can be applied by the community to increase income and welfare. This application of technology to society activity is held in Sambirejo, Jumantono, Karanganyar. The research was conducted from March to October 2016. The results showed that the application of chicken manure affects the number of leaves, number of tillers, and the fresh weight of turmeric rhizome. The addition of manure (chicken, goat, cow) can increase all variables of planting growth which include plant height, number of leaf, number of tillers, fresh weight of plant, dry weight of plant, and fresh weight of rhizomes. Giving three types of manure (chicken, goat, cow) with mycorrhizal dose treatment there is no intraction to some growth observation variables. Mycorrhizal treatment with a dose of 10 g/plant can increase the number of tillers in turmeric. Overall the application of mycorrhizal at various doses (5, 10, and 15 g/plant) can increase plant height, leaf number, number of tillers, and fresh weight of turmeric rhizome.


2020 ◽  
Vol 11 (1) ◽  
pp. 25
Author(s):  
AHMAD TAOFIK ◽  
SOFIYA HASANI ◽  
AYU CAHYANINGTYAS ◽  
BUDY FRASETYA

The paitan plant contains allelopathic compounds for other plants so that the utilize of paitan plant as a source of liquid organic fertilizer (LOF) has not been widely applied. This research aimed to study the interaction between LOF dosage and appropriate application method of LOF to increase the growth of kailan plants. The research conducted at experimental garden of UIN Bandung from June-August 2016. The experimental design was a completely randomized design with two factors and replicated four times. The first factor was application methods (A), i.e. a1=direct application to the soil, and a2= spraying. The second factor was LOF dosage (T), i.e. t0: 0 ml plant-1, t1: 20 ml plant-1, t2: 40 ml plant-1, t3: 60 ml plant-1, t4: 80 ml plant-1. The growth parameters observed i.e. plant height (14, 21, 28, 35 and 42 Day after Transplanting (DAT)), leaf area 42 DAT, plant dry weight 42 DAT, and plant fresh weight 42 DAT. The data then analyzed with analysis of variance at a significant level of 5%. If the analysis of variance was significant, the Duncan multiple range test was used at α=5%. The research results showed there was no interaction between method of application with a various dosage of paitan LOF to all of the growth parameters. The application paitan LOF showed similar growth in terms of plant height, leaf area, plant dry, and plant fresh weight. The application method of paitan LOF direct to the soil or spraying to leaves can not increase kailan plant growth.


2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Ghani Hendrika ◽  
Arifah Rahayu ◽  
Yanyan Mulyaningsih

This study was aimed at assessing the growth and production of celery plants grown with various compositions of N,P and K fertilizer and compost. The study was done at the Trial Farm of Faculty of Agriculture, Djuanda University, Bogor fiom September to November 2015. A completely randomized design with two factors was used. The factors consisted of compost rate and N,P and K fertilizer rate. Compost was given in four levels, namely no compost (0% R), 26.3% gcompost/plant (50% R), 52.5 g compost/plant (100% R), and 78.7 g compost/plant (150% R). N, P and K fertilizer was given in five levels, namely no fertilizer (0% R), 4 g Urea, 0.3 g SP-36, 0.2 g KCl (25% R), 0.9 g Urea, 0.6 g SP-36, 0.4 g KCI (50% R), 1.3 g Urea, 0.8 g SP-36, 0.6 g KCl (75% R), and 1.7 g Urea, 1.1 g SP-36, 0.8 g KCl (100% R). All fertilizers were administered gradually at the time of planting and 2 weeks after planting. Three replicates were allocated into each treatment making up 60 experimental units. Results showed that celery plants given synthetic fertilizer at 100% R had higher plant height, number of leaves, number of seedlings, harvest fresh weight (g), saleable fresh weight (g), root biomass (g), and root dry weight (g) than those treated with no fertilizer (0% R). Plants treated with 150% compost had higher plant height, number of leaves, harvest fresh weight (g), saleable fresh weight (g), root length (cm), and root dry weight (g) than those treated with no fertilizer (0% R). Thus the best celery growth and production in this study was obtained on plant 150% R compost   and 100% R synthetic fertilizer nourished.Key words: celery, compost, N, P, K fertilizer, harvest flesh weight


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