scholarly journals Phytochemical Screening, Antioxidant Activity and Phenolic Content of Different Plant Parts of Brucea javanica (L.)

2019 ◽  
Vol 14 (2) ◽  
pp. 33-43
Author(s):  
Hamidah Jaafar Sidek ◽  
Hidayatul Atiqah Abd Karim ◽  
Zurina Mahmud
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
The Other ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Brucea Javanica ◽  
Plant Parts ◽  
Ic50 Value

In this study, phytochemical screening, antioxidant activity as well as phenolic content in the leaves,twigs and barks of Brucea javanica (L.) were determined using standard phytochemical screeningmethod, DPPH radical scavenging assay and Folin-Ciocalteu reagent method, respectively. Methanolicextracts of these different plant parts were prepared by maceration method. Phytochemical screeningrevealed the presence of flavonoids, terpenoids and tannins in all plant parts. This test also revealed thepresence of alkaloids and saponins in all plant parts except they were absent in the twigs and barks parts,respectively. On the other hand, steroids was absent in all plant parts. DPPH method was used toevaluate the antioxidant properties of the plant parts by measuring the absorbance at 517 nm. Theantioxidant activity was then compared with standard ascorbic acid. Among all parts, methanolic extractof leaves of Brucea javanica (L.) exhibited the highest antioxidant activity at the concentration of 100ppm with 86.19 ± 0.20% inhibition which was comparable with standard ascorbic acid with 97.62 ±0.13% inhibition at the same concentration. The IC50 value of the methanolic extract of leaves was 54.52± 0.16 ppm while the other parts were more than 100 ppm. All plant parts showed IC50 value higher thanthe standard ascorbic acid which recorded IC50 of 9.04 ± 0.09 ppm. The methanolic extract of the leavesalso exhibited the highest total phenolic content which was 105.58 ± 0.21 mg GAE/g extract compared tothe other parts. This result correlated well with the higher antioxidant activity exhibited by themethanolic extract of the leaves. Thus, the leaves part of methanolic extract of Brucea javanica (L.)exhibited the highest antioxidant activity compared to the other plant parts. Keywords: Brucea javanica (L.), simaroubaceae, phytochemical screening, antioxidant activity, phenolic content

scholarly journals Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

2020 ◽  
Vol 27 (1) ◽  
pp. 061-066 ◽  
Author(s):  
Joyce Mamani ◽  
Jorge Chávez ◽  
Eder Apumayta ◽  
Patricia Gil-Kodaka
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanolic Extract ◽  
Total Phenol ◽  
North Coast ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
The North

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

scholarly journals Antioxidant, Anti-Diabetic and Phytochemical Screening of Dried Ficus carica Linn Fruit Powder

2020 ◽  
Vol 2 (1) ◽  
pp. 50
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Ic50 Value ◽  
Different Types

Ficus carica L. or the common name is red figs that belong to the Moraceae family or the Mulberry family. Every part of the fruit or the tree has been able to treat different types of common diseases, for example, it is used as a natural laxative or a supplementary food for diabetes. Thus, this research uses four different solvents, ethyl acetate, ethanol, hexane, and water, to obtain the respective crude extracts in order to investigate the anti-diabetic properties by determining the inhibition of the activity of the diabetic enzymes, α-amylase, and α-glucosidase; and the DPPH assay determines the antioxidant activity while qualitative phytochemical screening was for phenols, alkaloids, tannins, saponins, and flavonoids; total quantitative phenolic and total flavonoid content was done. The phytochemical screening showed the presence of the compounds tested in different types of crude extracts. For the total phenolic content, ethyl acetate extract exhibits the highest content. In contrast, hexane extract shows the highest total flavonoid content. For the DPPH assay, ethyl acetate extract has the highest scavenging activity at 13.351 µg/mL with corresponding with the data of total phenolic content. For the α-glucosidase inhibitory activity, water extract has the lowest IC50 value among the four extracts but higher value than the standard. For α-amylase inhibitory activity, only ethanol extract showed the IC50 value, but it was a high value. In conclusion, there is potential for figs to be a natural source of medicine, and the extracts tested can be used for future studies.

Pulicaria laciniata (Coss and Kral): Effects of Different Extraction Solvents on Phytochemical Screening and Antioxidant Activity

2021 ◽  
pp. 425-430
Author(s):  
Kamilia Bireche ◽  
Hocine Dendougui ◽  
Asma Abid ◽  
Abdeldjabbar Messaoudi ◽  
Mohamed Hadjadj
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
The Other ◽  
Phytochemical Screening ◽  
Butanol Extract ◽  
Metal Chelating ◽  
Phytochemical Investigation ◽  
Ic50 Value ◽  
Extraction Solvents ◽  
Phytochemical Constituents

This study aims to investigate phytochemical constituents of Pulicaria laciniata extracts and determine their antioxidant activity using three methods; Phosphomolybdate, Reducing Power, and Metal Chelating. The phytochemical investigation showed various secondary metabolites such as Phenols, Glycosides, Flavonoids, Alkaloids, Tannins, and Terpenoids. The N-butanol extract exhibited the highest antioxidant activity comparing with the other extract in all methods (0.51 and 0.65 mg/ml as A0.5 values of Phosphomolybdate, reducing power) and (1.65mg/ml for IC50 value of metal-chelating). In contrast, all the extracts showed week activity against the metal-chelating method.

scholarly journals ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE ACTION OF METHANOLIC EXTRACT OF LIQUORICE (GLYCYRRHIZA GLABRA) IN HEPG2 CELL LINE

2016 ◽  
Vol 8 (9) ◽  
pp. 293 ◽  
Author(s):  
Dasharath B. Shinde ◽  
Santosh S. Koratkar ◽  
Neeti Sharma ◽  
Ajinkya A. Shitole
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Hepg2 Cells ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Glycyrrhiza Glabra ◽  
Total Phenolic ◽  
Hepg2 Cell Line

<p><strong>Objective: </strong>To evaluate the <em>in vitro </em>antioxidant activity of liquorice (<em>Glycyrrhiza glabra) </em>against H<sub>2</sub>O<sub>2</sub> induced oxidative stress in HepG2 cell line.</p><p><strong>Methods: </strong>Antioxidant activity of methanolic extracts of <em>Glycyrrhiza glabra</em> was investigated by measuring total phenolic content using folin-ciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of <em>Glycyrrhiza glabra</em> against oxidative stress induced by H<sub>2</sub>O<sub>2 </sub>in HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H<sub>2</sub>O<sub>2</sub>, ascorbic acid, H<sub>2</sub>O<sub>2</sub>+liquorice and H<sub>2</sub>O<sub>2</sub>+ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels.<strong></strong></p><p><strong>Results: </strong>The total phenolic content estimated in <em>Glycyrrhiza glabra </em>extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H<sub>2</sub>O<sub>2 </sub>treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.</p><p><strong>Conclusion: </strong>Our results thus conclude that, the methanolic extract of <em>Glycyrrhiza glabra </em>can be used as natural supplements in various disease conditions where oxidative stress has been reported. <strong></strong></p><p> </p>

scholarly journals Antioxidant activity and phenolic acid content of selected vegetable broths

2017 ◽  
Vol 35 (No. 6) ◽  
pp. 469-475 ◽  
Author(s):  
Filiz Bilge Ertekin ◽  
Korkmaz Nazli ◽  
Budak Nilgun H ◽  
Seydim Atif C ◽  
Seydim Zeynep B Guzel
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Acid ◽  
Acid Content ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ascorbic Acid Content ◽  
Total Phenolic ◽  
Green Beans ◽  
Fresh Vegetables

The antioxidant activity and content of phenolic substances in vegetable broths were determined. Green beans, beetroots, courgettes, onions, parsley, carrots, cabbages, celery, broccoli, spinach, cauliflowers, and tomatoes were subjected to boiling. Fresh vegetables and vegetable broths were analysed for ascorbic acid content, total phenolic content, ORAC and TEAC values. Phenolic acids were quantified using HPLC. The ascorbic acid content of vegetables ranged from 5–109 mg/100 ml, while no ascorbic acids could be detected in vegetable broths. Total phenolic content was between 17–1729 mg GAE/l for all samples. ORAC and TEAC values of vegetable broths were between 0–3 µmol TE/ml and 0–2 µmol TE/ml, respectively. Gallic, chlorogenic, caffeic, p-coumaric, and ferulic acid were detected in both fresh vegetables and vegetable broths. The highest phenolic acid content was observed in water in which beetroots were boiled. It was found that the vegetable broths of beetroots, celery stalks, cabbages, parsley and broccoli harboured remarkable antioxidant activity.

scholarly journals Free-radical Scavenging and Quantitative Estimation of Flavonoids from the Polar Extracts of Corchorus depressus Leaves

2021 ◽  
pp. 210-219
Author(s):  
Khuntia Tapas Kumar ◽  
Nanda Upendra Nath ◽  
Senapati Aswini Kumar
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Nitric Oxide Radical ◽  
Polar Extracts

Background: The investigation of total flavonoids and antioxidant activity of polar extracts of Corchorus depressus is the major aim of this study. As observed from ancient literatures and folkloric claims the plant Corchorus depressus worshipped by the married women of Odisha, India, in the rituals called as “Jama Jutia”, possesses different biological activities including antioxidant property. Methods: The diphenyl picryl hydrazine, hydroxyl radical and nitric oxide radical scavenging methods were performed for measurement of the antioxidant activity at different extracts. The flavonoid and phenolic content of the extracts were determined by using aluminium chloride and Folin-Ciocalteau’s reagent (FCR) methods respectively. Results: The results for estimation of total phenolic content (mg/ 100 g) expressed as gallic acid equivalent (GAE) and total flavonoid (mg/ 100 g) in weight of quercetin equivalent (QE) was highest in methanolic extract 78.46 and  21.2 respectively, followed by 18.18 mg/100 g in GAE and 1.80 mg/100 g in QE for aqueous extract. Conclusion: The methanolic extract of C. depressus at 100µg/ml showed highest DPPH, hydroxyl and nitric oxide radical scavenging activity and this activity may be attributed to the presence of saponins and flavonoids as detected in the extract.

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