scholarly journals Sanitation at the Slaughterhouse and the Hygiene of Food of Animal Origin

2021 ◽  
Vol 9 (6) ◽  
Author(s):  
Mária Vargová ◽  
František Zigo ◽  
Katarína Veszelits Laktičová
Keyword(s):  
Food Safety ◽  
Food Processing ◽  
Raw Material ◽  
Coliform Bacteria ◽  
Animal Origin ◽  
Food Of Animal Origin ◽  
Food Borne ◽  
Colony Forming Units ◽  
Processing Plants ◽  
Food Borne Illness

Nowdays, one of the most important issues is the issue of food safety. There are many problems with the control of food safety and creation of appropriate legislation that protects food of animal origin. Hygiene and sanitation should be effectively applied and should be controlled at each step during production in food processing plants. The aim of study was to evaluate the surface microorganisms in the monitored parts of the slaughterhouse before slaughter and during slaughter but also after disinfection by disinfectant Virkon S. Disinfectant was used in a 1 % concentration and applied by spraying. Virkon S was effective on all monitored surfaces except the table for organs, where were detected 2x102 colony forming units per 10 cm2 of total count of bacteria, 2x102 colony forming units per 10cm2 of coliform bacteria and 1x102 colony forming unit per 10cm2 of moulds after disinfection. The sanitation program should be thoroughly planned, actively enforced, and effectively supervised. Disinfection has its meaning since, everything that comes into contact with the raw material can contribute to outbreaks of food borne illness.

scholarly journals Prevalence, Risk Factors, and Antibiogram of Nontyphoidal Salmonella from Beef in Ambo and Holeta Towns, Oromia Region, Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Endrias Zewdu Gebremedhin ◽  
Gezahegn Tafesse Soboka ◽  
Bizunesh Mideksa Borana ◽  
Lencho Megersa Marami ◽  
Edilu Jorga Sarba ◽  
...  
Keyword(s):  
Risk Factors ◽  
Food Safety ◽  
Antimicrobial Susceptibility ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Diffusion Method ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
Cross Sectional ◽  
Food Of Animal Origin

Background. Salmonella has been recognized as a major cause of food-borne illness associated with the consumption of food of animal origin. The present cross-sectional study was conducted from December 2017 to May 2018 in Ambo and Holeta towns to assess the prevalence, risk factors, and antimicrobial susceptibility patterns of nontyphoidal Salmonella isolates from raw beef samples from abattoirs, butchers, and restaurants in Ambo and Holeta towns, Oromia region, Ethiopia. Methods. A total of 354 beef samples were collected from abattoirs, butchers, and restaurants. Salmonella isolation and identification were carried out using standard bacteriological methods recommended by the International Organization for Standardization. Antimicrobial susceptibility testing was performed using the disk diffusion method. Besides, a structured questionnaire was used to collect sociodemographic data and potential risk factors for contamination of meat. Chi-square tests and logistic regression were used for data analyses. Results. Of the total 354 meat samples examined, 20 (5.7%, 95% confidence interval (CI): 3.5–8.6) were positive for Salmonella. Two serotypes belonging to S. typhimurium (11 isolates) and I:4,5,12: i:- (9 isolates) were identified. The Salmonella detection rate in abattoirs, butchers, and restaurants was 4.2% (5/118), 8.5% (10/118), and 4.2% (5/118), respectively. The antimicrobial susceptibility test showed that 40%, 30%, and 20% of the Salmonella isolates were resistant to azithromycin, amoxicillin, and ceftriaxone, respectively. The odds of Salmonella isolation when meat handlers are illiterate were 7.8 times higher than those when they are educated to the level of secondary and above ( P = 0.032 ). Similarly, the likelihood of Salmonella isolation was 6.3 and 7.6 times higher among workers of butcher and restaurants, respectively, who had no training ( P = 0.003 ) and no knowledge ( P = 0.010 ) on food safety and hygiene. Conclusions. The study showed widespread multidrug-resistant Salmonella isolates in the study areas. Therefore, raw meat consumption and indiscriminate use of antimicrobial drugs should be discouraged. Provision of food safety education for meat handlers and further surveillance of antimicrobial-resistant isolates are suggested.

scholarly journals Food safety in Bangladesh: A microbiological perspective

2017 ◽  
Vol 6 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Rashed Noor ◽  
Farahnaaz Feroz
Keyword(s):  
Food Safety ◽  
Present Review ◽  
Microbiological Contamination ◽  
Food Inspection ◽  
Legislative Action ◽  
Food Borne ◽  
Food Borne Illness ◽  
Regulatory Bodies

Bangladesh has long been facing problems associated with microbiological contamination of varieties foods due to the lack of awareness on hygiene followed by the defective legislative action. Manifestation of microbial prevalence in different foods bring about vital information on food safety as well as may envisage on the further intoxication caused by the prevailing microorganisms. Although a few extent of food inspection is apparently in some of the governmental regulatory bodies; however, the mass population is still suffering from a number of food borne illness. Present review partially focused on the microbiological regulation of the foods in Bangladesh and discussed the possible remedies for the maintenance of food safety.Stamford Journal of Microbiology, Vol.6(1) 2016: 1-6

scholarly journals Isolation of Shiga toxin-producing strains of Escherichia coli from beef and swine carcasses and the characterization of their genes

2018 ◽  
Vol 9 (2) ◽  
pp. 275-780 ◽  
Author(s):  
O. М. Berhilevych ◽  
V. V. Kasianchuk ◽  
O. M. Deriabin ◽  
M. D. Kukhtyn
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Raw Material ◽  
Animal Origin ◽  
E Coli ◽  
Food Of Animal Origin ◽  
Beef Carcasses ◽  
Positive Isolate ◽  
Stx1 Gene ◽  
Beef Carcass

Escherichia coli is part of the normal microflora of the intestinal tract of humans and warm-blooded animals, but its presence in raw material and food of animal origin is considered as fecal contamination and can be very dangerous for consumers. The determination of the number of E. coli in raw material and food is important because among them can be pathogenic strains. The most dangerous strains are considered enterohemorrhagic E. coli as a causative agent of severe bloody diarrhea and hemorrhagic uremic syndrome in humans through the production of Shiga-toxin, which is the main virulence factor, responsible for disease. The aim of this study was to identify the prevalence of Shiga toxin-producing strains of E. coli (STEC) from swabs of beef and swine carcass in slaughterhouses in Ukraine and characterize their genes, which are responsible for pathogenic properties. A total of 230 samples of swabs from beef (130) and swine (100) carcasses were obtained from 5 slaughterhouses in Ukraine between 2012 and 2015. Samples of swabs from carcasses were randomly selected at the final point of the process after the final washing of the carcass from the following areas: distal hind limb, abdomen (lateral and medial) from swine carcasses, brisket, flank and flank groin areas from beef carcasses. All samples were examined by culture-dependent method, after that each positive isolate of STEC was analyzed by multiplex PCR to detect the stx1, stx2, and eae genes. Out of 230 collected samples, seven (7.2%) were contaminated with STEC. The highest prevalence of STEC was found in swabs from beef carcasses (8.1%) in comparison to swabs from swine carcasses (5.7%). The stx1 gene was the predominant gene detected in all STEC positive samples. The eae gene was found in one of the examined isolates from beef carcass. Three isolates from swabs of beef carcass carried both stx1 and stx2 genes, one isolate showed association between stx1 and eae genes, one isolate was positive for stx1 gene only. In swabs from swine carcasses (2 isolates) stx1 and stx2 genes were presented simultaneously. The results of this study suggested that fresh raw meat could be a potential vehicle for transmission of the Shiga toxin-producing strain of E. coli to humans. This is the first report of STEC prevalence in beef and swine carcasses in Ukraine and these data will be valuable for microbiological risk assessment and help the appropriate services to develop strategies to mitigate health risk.

scholarly journals An epidemiological investigation of food-borne disease surveillance in the Ncera Villages, Eastern Cape, South Africa

2018 ◽  
Author(s):  
◽  
Khanya Zukolwakhe Bisholo
Keyword(s):  
South Africa ◽  
Food Safety ◽  
Medical Treatment ◽  
Healthcare Providers ◽  
Surveillance Systems ◽  
Eastern Cape ◽  
Food Borne ◽  
Food Borne Illness ◽  
Household Heads ◽  
At Home

An increase in food-borne disease burden in the world’s population has raised concerns over the reliability of surveillance systems. Research has shown the importance of food-borne surveillance systems used in the detection and management of food-borne illness. Government fiscals are increasingly burdened by the rapid spread of food-borne illness, although the exact economic impact is unclear in many countries. In recent years, food security has been the main agenda overshadowing food safety. A reactional approach to outbreaks is the trend instead of proactive systems. Food-borne disease is associated with low and high socio-economic status populations. More than 30 pathogens were identified as the major causes of food- borne outbreaks globally, and some food-borne pathogens have long term health consequences. Aim: The aim of the study was to investigate food-borne diseases surveillance in Ncera, Mpongo and Needscamp villages and local clinics, in the Eastern Cape, South Africa. Methodology: A retrospective, observational, quantitative study was conducted in two phases. The first phase included the screening of tick registers at Ncera, Mpongo and Needscamp clinics. The screening was to determine the number of food-borne cases that were reported at these clinics. In the second phase, a stratified random sampling method was used to interview 90 households from the above-mentioned villages to determine the number of villagers who suffered from food-borne diseases, symptoms experienced and food safety practices. Results from both phases were compared to determine whether the number of reported cases at the clinics reflected the same number of cases in the villages from 2012 to 2014. The total size of the study population was 5007 people. Respondents were invited to participate having signed informed consent. Data was summarised and described using descriptive statistics such as frequencies, means and standard deviations. Data was analysed using SPSS version 23; cross tabulations and Chi-square tests at a probability of p< 0.05 were done. Graphs and tables were used to graphically represent the data. Results: It was found that the majority of household heads were female (n = 51; 58.6%) and 33 (37.9%) of them were married. Most of the residents (n = 84; 96.5%) use the public health clinics for their medical condition treatment. Fifty-six (64.4%) household heads were HIV negative. The majority of households had a monthly income of R1 500 – R 3 500 (n = 45; 51.7). Less than a tenth (n = 6; 6.9%) of household heads were very concerned about the safety of food prepared at home. The relationship between food safety concern levels about food prepared at home and away from home was statistically significant (p = 0.000), reporting a significant difference in the way people perceive the preparation of food at home and away from home. More than a tenth of the villagers (n = 79; 19.7%) reported through the questionnaire, that they fell ill or thought that they fell ill from something they ate in the past 3 months. More than half (n = 56; 51.3%) of the participants who fell ill with food- borne diseases in these villages did not seek medical treatment for their illness whilst 6 (54.6%) did not see the need to seek medical treatment and reported that they got ill during weekends. Of those who sought medical treatment, 16 (39%) received prescribed medication while 3 (7%) reported that they were not provided with medication by healthcare providers when they suffered from food-borne illness. More than a quarter (n = 109; 27.3%) of household members fell ill from food-borne diseases in Ncera, Mpongo and Needscamp villages during the period 2012 to 2014. Whereas there were four food-borne cases reported to the clinics in the same period. Conclusion: This research gathered information regarding food-borne disease prevalence in Ncera, Mpongo and Needscamp villages. It was observed that there is a gap in the surveillance of food-borne illness in these villages. In some of the tick registers used by healthcare providers at clinics to collect data, vital surveillance information such as gender, age and diagnosis was missing. This study deepens the understanding of food-borne illness and food safety in a village setting.

scholarly journals Effect of Atmospheric Pressure Plasma on Listeria monocytogenes Attached to Abiotic Surfaces

2019 ◽  
Vol 82 (2) ◽  
pp. 233-237 ◽  
Author(s):  
VALENTINA ALESSANDRIA ◽  
KALLIOPI RANTSIOU ◽  
MARIA CHIARA CAVALLERO ◽  
LUCA SIMONE COCOLIN
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Atmospheric Pressure ◽  
Brain Heart Infusion ◽  
Atmospheric Pressure Plasma ◽  
Raw Material ◽  
Pressure Plasma ◽  
Abiotic Surfaces ◽  
Processing Plants ◽  
The Individual

ABSTRACT Listeria monocytogenes can be introduced into food processing plants via raw material of animal or plant origin and can establish endemic populations through formation of biofilms. Biofilms are a continuous source of contamination for food products, and L. monocytogenes cells in biofilms are more resistant to stress and sanitizing agents than are planktonic cells. The use of gas-discharge plasmas may offer a feasible alternative to conventional sanitization methods. Plasmas are a mixture of charged particles, chemically reactive species, and UV radiation and can be used to destroy microorganisms. The purpose of this study was to measure the effectiveness of cold atmospheric pressure plasma (APP) treatments against bacteria attached to a solid surface and to evaluate the individual susceptibility of various L. monocytogenes strains. Attention was focused on the state of the cells after treatment, combining detection by viable counts and quantitative PCR (qPCR). Most of the culturable cells were inactivated after APP treatment, but the qPCR assay targeting the 16S rRNA revealed the presence of injured cells or their entrance into the viable but nonculturable state. These results were at least partly confirmed by a resuscitation experiment. After APP treatment, L. monocytogenes cell suspensions were incubated in brain heart infusion broth; some cells grew in the medium and therefore had survived the treatment. An understanding of the effects of APP on L. monocytogenes can inform the development of sanitation programs incorporating APP for pathogen removal. Methods other than those based of the culturability of the cells should be used to monitor pathogens in food processing plants because cultivation alone may underestimate the actual microbial load.

scholarly journals Chitin Hydrolysis by Listeria spp., Including L. monocytogenes

2008 ◽  
Vol 74 (12) ◽  
pp. 3823-3830 ◽  
Author(s):  
J. J. Leisner ◽  
M. H. Larsen ◽  
R. L. Jørgensen ◽  
L. Brøndsted ◽  
L. E. Thomsen ◽  
...  
Keyword(s):  
Food Processing ◽  
Cell Walls ◽  
Chitinolytic Activity ◽  
Gene Encoding ◽  
Carbon And Nitrogen ◽  
Food Borne ◽  
Genes Encoding ◽  
Processing Plants ◽  
Chitin Hydrolysis ◽  
Chitin Binding Protein

ABSTRACT Listeria spp., including the food-borne pathogen Listeria monocytogenes, are ubiquitous microorganisms in the environment and thus are difficult to exclude from food processing plants. The factors that contribute to their multiplication and survival in nature are not well understood, but the ability to catabolize various carbohydrates is likely to be very important. One major source of carbon and nitrogen in nature is chitin, an insoluble linear β-1,4-linked polymer of N-acetylglucosamine (GlcNAc). Chitin is found in cell walls of fungi and certain algae, in the cuticles of arthropods, and in shells and radulae of molluscs. In the present study, we demonstrated that L. monocytogenes and other Listeria spp. are able to hydrolyze α-chitin. The chitinolytic activity is repressed by the presence of glucose in the medium, suggesting that chitinolytic activity is subjected to catabolite repression. Activity is also regulated by temperature and is higher at 30°C than at 37°C. In L. monocytogenes EGD, chitin hydrolysis depends on genes encoding two chitinases, lmo0105 (chiB) and lmo1883 (chiA), but not on a gene encoding a putative chitin binding protein (lmo2467). The chiB and chiA genes are phylogenetically related to various well-characterized chitinases. The potential biological implications of chitinolytic activity of Listeria are discussed.

scholarly journals Detection of PathogenicEscherichia coliandStaphylococcus aureusfrom Cattle and Pigs Slaughtered in Abattoirs in Vhembe District, South Africa

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Nicoline F. Tanih ◽  
Eunice Sekwadi ◽  
Roland N. Ndip ◽  
Pascal O. Bessong
Keyword(s):  
Animal Origin ◽  
Isolation Rate ◽  
E Coli ◽  
Food Of Animal Origin ◽  
Food Borne ◽  
Microbiological Methods ◽  
Significant Difference ◽  
Resistant Strains ◽  
Vhembe District ◽  
Epidemiological Significance

Pathogenic food-borne bacteria have been associated with severe morbidity and mortality in humans and animals. This study was aimed at determining the prevalence ofStaphylococcus aureus, Salmonellaspp., andEscherichia colipresent in cattle and pigs slaughtered in selected abattoirs in Vhembe District and at determining the susceptibility of the isolates to antibiotics. A total of 176 swab samples (28 cattle and 16 pigs) of the rump, flank, brisket, and neck of the animals were analyzed using standard microbiological methods.E. coliisolates were genotyped to detect pathogenic strains. Of the 176 samples, 104 (67.5%) were positive forE. coliand 50 (32.5%) forS. aureus. There was no statistically significant difference(P>0.05)in the isolation rate from the different animal parts or abattoirs. Overall, 14/104 (13.46%) of theE. coliisolates were pathogenic strains which included enteropathogenicE. coli(EPEC) (bfpA) 1.9%, enterotoxigenicE. coli(ETEC) (LT) 3.8%, and enteroaggregativeE. coli(EAEC) (aaiC) 7.6%.E. coliisolates were resistant (100%) to vancomycin and bacitracin.S. aureus(100%) were resistant to oxacillin and nalidixic acid. The presence of resistant strains of these bacteria in food of animal origin could serve as important vehicles transmitting these bacteria to humans. This finding is of epidemiological significance.

scholarly journals An assessment of the microbiological quality of lightly cooked food (including sous-vide) at the point of consumption in England

2017 ◽  
Vol 145 (7) ◽  
pp. 1500-1509 ◽  
Author(s):  
F. JØRGENSEN ◽  
L. SADLER-REEVES ◽  
J. SHORE ◽  
H. AIRD ◽  
N. ELVISS ◽  
...  
Keyword(s):  
Microbiological Quality ◽  
Chicken Liver ◽  
Animal Origin ◽  
Protection Agency ◽  
E Coli ◽  
Food Of Animal Origin ◽  
Colony Forming Units ◽  
Cooked Food ◽  
Foie Gras

SUMMARYThis observational study aims to investigate the microbiological quality of commercially prepared lightly cooked foods with a major component of food of animal origin and collected as would be served to a consumer. A total of 356 samples were collected from catering (92%), retail (7%) or producers (1%) and all were independent of known incidents of foodborne illness. Using standard methods, all samples were tested for: the presence of Campylobacter spp. and Salmonella spp. and enumerated for levels of, Bacillus spp. including B. cereus, Clostridium perfringens, Listeria spp. including L. monocytogenes, Staphylococcus aureus, Escherichia coli, Enterobacteriacea and aerobic colony count (ACC). Results were interpreted as unsatisfactory, borderline or satisfactory according to the Health Protection Agency guidelines for assessing the microbiological safety of ready-to-eat foods placed on the market. Amongst all samples, 70% were classified as satisfactory, 18% were borderline and 12% were of unsatisfactory microbiological quality. Amongst the unsatisfactory samples, six (2%) were potentially injurious to health due to the presence of: Salmonella spp. (one duck breast); Campylobacter spp. (two duck breast and one chicken liver pâté); L. monocytogenes at 4·3 × 103 cfu (colony-forming units)/g (one duck confit with foie gras ballotin) and C. perfringens at 2·5 × 105 cfu/g (one chicken liver pâté). The remaining unsatisfactory samples were due to high levels of indicator E. coli, Enterobacteriaceae or ACC.

The Transmission of Disease via Foods of Animal Origin

1995 ◽  
Vol 24 (1) ◽  
pp. 23-25 ◽  
Author(s):  
J.G. Van Logtestijn ◽  
H.A.P. Urlings
Keyword(s):  
International Trade ◽  
Human Health ◽  
Food Processing ◽  
Preventive Measures ◽  
Animal Origin ◽  
Production Methods ◽  
Food Borne ◽  
Immune Deficiencies ◽  
Human Immune ◽  
Transmission Of Disease

Foods of animal origin have always posed some risk to human health when they have not been processed properly, but the incidence of food-borne diseases has been increasing recently due to changes in animal production methods, food processing technology, increased international trade and travel, and human immune deficiencies. In addition new diseases are arising, both variations on the old ones and novel threats such as BSE. These diseases are surveyed here, with an accent on preventive measures.

Sign in / Sign up

Export Citation Format

Share Document