scholarly journals EVALUATION OF PLEURA EFUSION DETERMINATION BY LIGHT’S DAN HEFFNER’S CRITERIA

2019 ◽  
Vol 26 (1) ◽  
pp. 76
Author(s):  
Nor Jannah Ali ◽  
Ani Kartini Dr ◽  
Darmawaty Effendi Dr
Keyword(s):  
Pleural Effusion ◽  
Sensitivity And Specificity ◽  
Clinical Pathology ◽  
Medical Doctor ◽  
University Hospital ◽  
Fluid Accumulation ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Cholesterol Levels ◽  
Effusion Fluid

EVALUATION OF  PLEURA EFUSION DETERMINATION BY LIGHT’S DAN HEFFNER’S CRITERIANordjannah1, Ani Kartini2, Darmawaty ER 31 Medical Doctor Specialist Education Programe of Clinical Pathology, Faculty of Medicine Hasanuddin University/dr.Wahidin Sudirohusodo Hospital, Makassar2  Department of Clinical Pathology Faculty of Medicine, Hasanuddin University/ Labuang Baji Hospital  Makassar3 Department of Clinical Pathology Faculty of Medicine, Hasanuddin University/ Hospital Islam Faisal Hospital  Makassar  ABSTRACT Background : Pleural effusion is a condition of abnormal pleural fluid accumulation in the pleural cavity due to excessive transudation or exudation. Light’s criteria is used as the standard method to distinguish between exudates and transudates. Some recent studies reported misclassifications so several alternative criteria are developed, one of which is Heffner’s criteria. The purpose of this study was to determine the sensitivity and specificity of Heffner’s criteria in determining the type of pleural effusion.Methods : An observational study with cross sectional method using a pleural effusion fluid sample of patients examined at the Clinical Pathology Laboratory Instalation at Wahidin Sudirohusodo Hospital on July 2018. Total protein, LDH and cholesterol levels were examined in all samples that met the inclusion and exclusion criteria.Results : There were 45 samples of pleural effusion, 30 of which classified as transudate and 15 samples as exudates. Based on clinical diagnosis, the Light’s criteria obtained 3 misclassifications and Heffner’s criteria obtained 2 misclassifications. Based on the data above, the statistical data showed that Light’s criteria has sensitivity of 96,7 % and specificity of 86,7 %. Heffner’s criteria has sensitivity of 100 % and specificity of 86,7 %.  Conclusion : Heffner’s criteria offers better sensitivity and specificity than Light’s criteria. Heffner’s criteria can be used as an alternative in determining the type of pleural effusion Keywords: Heffner’s criteria, Light’s criteria, transudate, exudate, pleural effusion 

scholarly journals DIAGNOSTIC VALUE OF URIC ACID IN PLEURAL EFFUSION

2018 ◽  
Vol 24 (2) ◽  
pp. 146
Author(s):  
Saraswati Wulandari Hartono ◽  
Nurhayana Sennang ◽  
Fitriani Mangarengi
Keyword(s):  
Uric Acid ◽  
Pleural Effusion ◽  
Pleural Fluid ◽  
Sensitivity And Specificity ◽  
Uric Acid Level ◽  
Acid Level ◽  
Clinical Pathology ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Significant Difference

Pleural effusion is excessive pleural fluid accumulation in the aimed pleural cavity, are categorized into exudate and transudate. Light’s criteria (1972) has become a classic criteria to distinguish pleural effusion types. Other criteria were evaluated such as pleural fluid uric acid. The aimed of this study was to analyze the difference of uric acid level between transudate and exudate and to compare it with Light’s criteria. A cross-sectional study was conducted in Clinical Pathology Laboratory of the Dr. Wahidin Sudirohusodo Hospital Makassar in September 2016. Uric acid level of pleural effusion samples was measured by ABX Pentra 400. Statistical analysis used Mann-Whitney U test, significance was indicated if p<0.05. Cut-off, sensitivity and specificity of uric acid used ROC curve. Total subjects were 56 patients, mean age 49.54+13.63 years-old, 31 males (55.4%) and 25 females (44.6%). Most cases were exudative effusion (58.9%) with 19 malignancy cases (33.9%). Uric acid level median was 6.6 mg/dL (3.24-17.50 mg/dL) higher in transudate than exudate 5.01 mg/dL (0.6-9.40 mg/dL) (p=0.001). The cut-off point for pleural fluid uric acid was 5.845 mg/dL, with a sensitivity of 78.3% and specificity of 66.7%. Sensitivity and specificity of Light’s criteria was 97% and 60.9%. There was a significant difference between pleural fluid uric acid level in transudate and exudate, higher in transudate than exudate. Light’s criteria were higher in sensitivity than uric acid, but lower in specificity. Further research is needed with better sampling method to reduce bias. 

scholarly journals ANALISIS CAIRAN DARAH (TRANSUDAT) DAN SERUM CAMPURAN (EKSUDAT) DI PENDERITA DENGAN REMBESAN SELAPUT PARU (EFUSI PLEURA)

2018 ◽  
Vol 15 (2) ◽  
pp. 57
Author(s):  
Didi Irwadi ◽  
Sulina Y. Wibawa ◽  
Hardjoeno Hardjoeno
Keyword(s):  
Pleural Effusion ◽  
Leukocyte Count ◽  
Clinical Pathology ◽  
Cross Sectional Study ◽  
T Test ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Old Patients ◽  
Acinetobacter Spp ◽  
Staphylococcus Spp

Pleural effusion is a fluid excess into pleural cavity due to transudation or exudation processes. The fluid deposited in the cavity canthreat the patient’s life. The pleural effusion could be produced in a patient with tuberculosis, cancer, cardiac failure, renal failure orviral/bacterial infection. The study is aimed to analyze the patterns of substance in the pleural effusion fluids produced by differentdiseases. A cross sectional study was performed from June 2006 to June 2007 at Clinical Pathology Laboratory of Dr. WahidinSudirohusodo Hospital, Makassar. The fluids were tested for glucose, total protein, LDH, and leukocyte count. Of 87 pleural effusion fluidsamples from 14–80 years old patients, 34.5% were transudates and 65.5% were exudates. Glucose value was higher (one tail T test,p < 0.01) in transudates group, whereas protein, LDH and leukocyte count were higher (one tail T test, p < 0.01) in exudates group.There were no significant differences of glucose, protein, LDH and leukocyte count among diseases within transudates group, as well aswithin exudates group. Staphylococcus spp., Klebsiella spp., and Acinetobacter spp., were the predominant bacteria revealed from thefluid cultures. Values of glucose, protein, LDH and leukocyte count have a different pattern between transudates and exudates groups.However, no special patterns were found among diseases within groups.

scholarly journals TOTAL CHOLESTEROL ANALYSIS FOR DIFFERENTIATING EXUDATES AND TRANSUDATES IN PLEURAL FLUIDS

2018 ◽  
Vol 24 (2) ◽  
pp. 136
Author(s):  
Efelina Sutanto ◽  
Liong Boy Kurniawan ◽  
Fitriani Mangarengi
Keyword(s):  
Total Cholesterol ◽  
Pleural Fluid ◽  
Predictive Value ◽  
Total Cholesterol Level ◽  
Clinical Pathology ◽  
Diagnostic Value ◽  
Cross Sectional Study ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Cholesterol Levels

The aimed of this study is to compare the diagnostic value of pleural fluid total cholesterol and Light’s criteria to determine exudate or transudate. The samples used in this cross-sectional study were pleural fluid specimens sent to the Clinical Pathology Laboratory of the Dr.Wahidin Sudirohusodo Hospital Makassar during the period of August-September 2016. Data were grouped according to the type of effusion then statistically analyzed using nonparametric Mann Whitney U-test. The result of this study showed from 55 samples, there were 22 transudates and 33 exudates. The mean total cholesterol levels of exudate is higher than transudate (p=0.006). By using cut-off value of total cholesterol pleural fluid 56 mg/dL, it had sensitivity 72.7%; specificity 78.8%; Positive Predictive Value (PPV) 81.3% and Negative Predictive Value (NPV) 69.6% while Light’s criteria had sensitivity 97%; specificity 63.6%; PPV 80% and NPV 93.3%. Pleural fluid total cholesterol level with cut-off 56 mg/dL is proposed to be used for differentiating exudate and transudate because it is easier and more simple to perform than Light’s criteria.  

scholarly journals THE DIAGNOSTIC VALUE OF TROPONIN I TESTING TO CORONARY ANGIOGRAPHY WITH A POINT OF CARE TESTING INSTRUMENT IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION

2019 ◽  
Vol 25 (1) ◽  
pp. 64
Author(s):  
Riska Anton ◽  
Sheila Febriana ◽  
Asvin Nurulita ◽  
Uleng Bahrun
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Muscle ◽  
Sensitivity And Specificity ◽  
Troponin I ◽  
Clinical Pathology ◽  
Rapid Test ◽  
Diagnostic Value ◽  
Pathology Laboratory ◽  
Cross Sectional

Myocardial infarction consists of STEMI and NSTEMI. Acute myocardial infarction is diagnosed by WHO criteria when at least two of the following three criteria are met: chest pain, electrocardiography (ECG) result changes, and biomarker. Troponin I is specific for cardiac muscle and has an increased level even in small cardiac muscle necrosis and not affected by the renal failure and muscle trauma but have not been standardized by WHO. This research aimed to find the effectivity of Troponin I examination with POCT to help the diagnosis and early detection of AMI. Thus each product has varied sensitivity and specificity. A cross-sectional study was conducted in the Clinical Pathology Laboratory and Cardiac Center of the Dr. Wahidin Sudirohusodo Hospital Makassar using suspected AMI patients as the subject. Troponin I level tested by POCT from August 2015 to July 2016. Data were analyzed statistically using the ROC curve with SPSS software. A total of 88 patients suspected with AMI, aged 36 to 75 years old. From the tested cut-off values (0.02, 0.03, 0.04, 0.5, 0.06, 0.07, 0.08 μg/L) the best cut-off value was 0.03 μg/L (93.9% sensitivity, 95.5% specificity, PPV 98.4%, NPV 84.0%, and 94.3% accuracy) where the cut-off value of 0.03 μg/L was the value recommended by the toolkit manual. Even if the cut-off value of 0.02 or 0.04 was used, the sensitivity and specificity value was still fairly good. Troponin I testing using POCT with a cut-off value of 0.03 μg/L can be used routinely in supporting the AMI diagnosis because it is a rapid test with a portable instrument and excellent diagnostic value.

Comparison of Three Different Tests for Assessment of Hepatitis C Virus in Dialysis Patients

1995 ◽  
Vol 15 (3) ◽  
pp. 241-245 ◽  
Author(s):  
Claudia Castelnovo ◽  
Giovanna Lunghi ◽  
Amedeo De Vecchi ◽  
Anna Grancini ◽  
Giovanna Como ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Sensitivity And Specificity ◽  
Cross Sectional Study ◽  
University Hospital ◽  
Dialysis Patients ◽  
Cross Sectional ◽  
Dialysis Unit ◽  
Recombinant Immunoblot Assay ◽  
Virus C

Objective To evaluate the relationship between hepatitis C virus antibodies (HCV-Ab) and viremia and to compare the prevalence of HCV-Ab and HCV viremia in hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients. Design Cross-sectional study. Setting Dialysis unit of a nephrology division in a public university hospital. Patients All dialysis patients who came for routine clinic visits during the study period. None denied informed consent. Forty-eight patients on HD and 79 on CAPD were examined. Intervention Blood samples were tested by second generation enzyme-Iinked immunosorbent assay (ELISA II) and recombinant immunoblot assay (RIBA II) to look for HCV-Ab and by the polymerase chain reaction (PCR) to look for HCV viremia. Results ELISA II was positive in 52% of HD patients and in 14% of CAPD patients. RIBA II was positive in 48% of HD patients and in 11% of CAPD patients. HCV viremia was positive by PCR in 41.6% of HD patients and in 12% of CAPD patients. Two of these PCR-positive patients did not show HCV-Ab by ELISA II and RIBA II. The sensitivity and specificity of ELISA II were 93% and 92%, the sensitivity and specificity of RIBA II were 86% and 94%. Conclusions Our data confirm a higher prevalence of HCV viremia in HD than in CAPD patients. The absence of Ab against virus C in 2 patients positive with PCR might be due to recent HCV infection or to weak virus replication or to a poor immune response.

scholarly journals ANALISIS LAMA WAKTU PELAYANAN LABORATORIUM DI RUMAH SAKIT UMUM DAERAH PASAMAN BARAT

2018 ◽  
Vol 5 (1) ◽  
pp. 114-121
Author(s):  
Betti Rosita ◽  
Ulfa Khairani
Keyword(s):  
Health Services ◽  
Waiting Time ◽  
Service Time ◽  
Clinical Chemistry ◽  
Clinical Pathology ◽  
Blood Chemistry ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Laboratory Service ◽  
Laboratory Services

Hospital laboratory services is one of the activities in hospitals that support quality health services. One indicator of the success of health services in the field of clinical pathology laboratory services is the waiting time for laboratory services. The standard set for laboratory service outcome waiting time is ≤ 140 minutes for blood chemistry and routine blood. This research analyzed the service time of outpatient laboratory that conducted hematology and clinical chemistry examination in the laboratory of Pasaman Barat Hospital. This research is a type of descriptive analytic research that is quantitative and supported by qualitative using cross sectional approach. The result of the research showed that laboratory service time at RSUD Pasaman Barat fulfilled the standard (≤ 140 minutes) for hematology examination 33.94 minutes, clinical chemistry 83.92 minutes, hematology and clinical chemistry 98 minutes. Based on the results of this study, the stages that contribute to the length of time laboratory services for hematological examination lies in the pre analytical stage, for clinical chemistry examination is located at the analytical stage, as well as for hematology and clinical chemistry examination lies in the analytic stage as well as the factors that affect the length of time laboratory services are human resources that is in the ability of officers and infrastructure facilities

scholarly journals Study of renal functions in metabolic syndrome and its correlation with various parameters

2020 ◽  
Vol 8 (3) ◽  
pp. 960
Author(s):  
Anand N. ◽  
Vidya T. A.
Keyword(s):  
Metabolic Syndrome ◽  
Renal Function ◽  
Diastolic Pressure ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Cross Sectional Study ◽  
University Hospital ◽  
Cross Sectional ◽  
Renal Functions ◽  
Cholesterol Levels

Background: Metabolic syndrome includes a constellation of various metabolic abnormalities that have been associated with cardiovascular disease, stroke and all-cause mortality in the general population. It has now been established that it is also associated with renal dysfunction. This study was done to assess renal function in metabolic syndrome in Indian population as well as its correlation with different parameters of the metabolic syndrome.Methods: A cross-sectional study was conducted in a university hospital from 2014 - 2015. Renal functions were studied in 100 obese individuals, 50 with and 50 without metabolic syndrome after informed consent and the results were analysed.Results: 100 obese individuals, 50 with and 50 without metabolic syndrome were compared. All underwent a physical examination and relevant investigations. All parameters of renal function showed significant derangement in cases as compared to controls. 38 patients (76%) among the cases (N=50) of metabolic syndrome had altered renal functions versus 9 patients (18%) among controls (n=50). Individually, there was a significant correlation of altered renal function (reduced estimated glomerular filtration rate eGFR and presence of microalbuminuria) with fasting blood glucose and systolic blood pressure (p=0.001), diastolic pressure (p=0.003) and triglyceride levels (p=0.036). High density lipoprotein-cholesterol levels did not show a significant correlation.Conclusions: Obese individuals with metabolic syndrome have significant derangement of renal functions as compared to those without metabolic syndrome. Most parameters of the syndrome are also independently associated with alteration of renal functions.

ANGKA BANDING ALBUMIN KREATININ AIR KEMIH DAN HbA1C SERTA ESTIMASI LAJU FILTRASI GLOMERULUS PADA PASIEN DIABETES MELITUS TIPE 2

2016 ◽  
Vol 21 (3) ◽  
pp. 250
Author(s):  
Amiroh Kurniati ◽  
Tahono Tahono
Keyword(s):  
Glycemic Control ◽  
Clinical Pathology ◽  
Poor Glycemic Control ◽  
Control Group ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Chronic Hyperglycemia ◽  
Type 2 Dm ◽  
Spearman Correlation Test

Diabetes Mellitus (DM) type 2 is a metabolic disease that prevalence increasing. A chronic hyperglycemia with poor glycemic control can stimulate oxidative stress, which will continue to occurrence of complications in the kidneys characterized by the presenceof microalbuminuria can be measured by the ratio of urinary albumin creatinine ratio (UACR) and the change in estimated glomerular filtration rate (eGFR). The aims of this study was to know the correlation between the UACR with HbA1c value and eGFR in patients with type 2 DM by finding them out. This study used cross sectional research design. Subjects were patients with type 2 DM who attend control in Endocrinology Subdivision of Internal Medicine Departement and perform blood and urine tests in Clinical Pathology Laboratory in Dr. Moewardi Hospital Surakarta in August 2013. To determine the pattern of the data distribution, the researchers used KolmogorovSmirnov test, and to analyse the result used Spearman (r) correlation with p<0.05 and confidence interval 95%. Statistical analysis using Spearman correlation test (r), significant when p<0.05 with 95% confidence intervals. From 68 samples examined the mean age is 60.9 year old, with equal participants for male and female (34 subjects each). Most subjects were in poor glycemic control group (72.1%) and in the range of microalbuminuria (44.1%). There was a significant correlation between UACR with HbA1c and eGFR in type 2 DM patient (r=0.412, p=0.000; and r= -0.270, p=0.02, respectively). Based on this study it can be concluded that increased UACR were associated with worsened glycemic control and were characterized by higher levels of HbA1c and its eGFR value would be lower. Further analysis requires further research with a larger sample size and more attention to the factors that may affect the related examination

scholarly journals DETERMINATION OF GRAY ZONE RANGE IN HBsAg TEST AND ITS IMPLICATION FOR HBsAg CONFIRMATION TEST

2018 ◽  
Vol 24 (2) ◽  
pp. 127
Author(s):  
Vina Zakiah Latuconsina ◽  
Irda Handayani ◽  
Asvin Nurulita ◽  
Uleng Bahrun Uleng Bahrun
Keyword(s):  
False Positive ◽  
False Negative ◽  
Clinical Pathology ◽  
Cross Sectional Study ◽  
Gray Zone ◽  
Chi Square ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Confirmation Test ◽  
Chi Square Test

Hepatitis is an inflammation of liver cells caused by infection (virus, bacteria, paracytes), medication, alcohol consumption, excessive lipid and autoimmune disease. Increasing method (sensitivity) of HBsAg test is often followed by a lot of false positive test results, which need a confirmation test which takes a longer time and higher cost so that it is needed to determine an optimal gray zone range for a confirmation test. This analytical cross-sectional study was held at the Clinical Pathology Laboratory of the Dr.Wahidin Sudirohusodo Hospital Makassar. The subjects were specimens which HBsAg value was within 0.06-1 COI. Specimens were examined using HBsAg confirmation test. Over 49 samples collected there were 32 reactive and 17 non-reactive. 14 out of 32 reactive samples (43.8%) were confirmed as negative (false positive) by HBsAg confirmation test and 3 out of 17 non-reactive samples (17.6%) were confirmed as positive (false negative). Chi-square test showed a significant correlation between HBsAg value and HBsAg confirmation test value (p=0.009). Chi-square test with some intervals with α=1% showed that the interval of HBsAg value 0.13-0.17 COI was the interval with the lowest significant value (p=0.004). This subsequent interval was recommended as the gray zone range. The range 0.13-0.17 was most optimal as HBsAg gray zone to determine the need for a confirmation test. A further study with larger samples is suggested. 

Sign in / Sign up

Export Citation Format

Share Document