scholarly journals The Effect of Immune Max in the Liver, Kidney and Spleen Histological Structure in Male Albino Mice

2021 ◽  
Vol 53 (03) ◽  
pp. 186-189
Author(s):  
Mohammed Abed Jawad ◽  
Keyword(s):  
Dietary Supplement ◽  
Histological Examination ◽  
Nutritional Supplement ◽  
Treated Group ◽  
Histological Structure ◽  
Male Mice ◽  
Histological Changes ◽  
Dose Concentration ◽  
Albino Mice

Background: This study aimed to identify the effect of using the Immune Max dietary supplement on the histological composition of some organs in male albino mice. Methods: The study was conducted on the Swiss albino male mice which were divided into two groups: treated group with 0.162 mg/kg/day of Immune Max and second group as a control. Results: Histological examination was conducted for liver, kidney and spleen. The results showed some histological changes in these organs like loses of tissues, congestion, leukocytes infiltration and dead cells. Conclusion: The purpose of a conclusion that the use of the Immune Max nutritional supplement can cause damage to the tissues of a number of organs and this is likely to be due to the dose concentration and the duration of the treatment.

scholarly journals Study the Effects of Methotrexate with and without Vitamin A on Some Biochemical and Histological Parameters in Male Rabbits

2017 ◽  
Vol 11 (1) ◽  
pp. 45-53
Author(s):  
Makarim Q. Al-Lami ◽  
Asmaa I. Sail ◽  
Salah M. Al-Chalabi ◽  
Ferial A. Al-Mahdawi
Keyword(s):  
Vitamin A ◽  
Histological Examination ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Treated Group ◽  
Control Group ◽  
Low Density ◽  
Typical Structure ◽  
Histological Changes ◽  
Sinusoidal Dilatation

The present study aims to evaluate the effects of methotrexate (MTX) with and without vitamin A (Vit. A) on some biochemical parameters and histological structure in male rabbits liver. Twenty male rabbits weighing 1250-1480 gm were divided into four equal number groups. The first group was given 2 ml distilled water as control group. The second group was given MTX (20 mg/kg), the third group was given Vit. A (5000 IU), while the fourth group was given MTX (20 mg/kg) +Vit. A (5000 IU) in alternative days. Following four weeks of treatment, lipid profile total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), [low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL)]; in addition to thyroid hormones triiodothyronine (T3) and thyroxin (T4)] and liver enzymes [glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT)] were determined in the serum. Also, the histological examination of liver of all the experimental groups were carried out. The results were revealed that the treatment with MTX caused a significant P≤0.05 increases in TC, HDL, LDL, T4, and GPT when compared with the control group. The treatment with Vit. A did not cause any significant P≥0.05 differences in all the studied parameters. The MTX+Vit. A treated group showed a significant P≤0.05 increases only in GPT compared with the control group; while a significant P≤0.05 decreases was found in TC, HDL, T3, T4, and GOT when compared with the MTX treated group. The histological examination of the liver sections showed that MTX administration caused major histological changes in comparison with the control such as inflammatory cell infiltrations, vascular congestion, sinusoidal dilatation and granular degeneration of hepatocytes. Treatment with Vit. A showed a typical structure in liver tissue. While in MTX+Vit. A group, the histological changes were less severe than those in the MTX treated group; these changes were granular degeneration of hepatocytes and sinusoidal dilatation at low levels. The overall results of this study confirmed that administration of Vit. A decreased the side effects of MTX; this protective effect of Vit. A may have clinical applications in chemotherapy.

scholarly journals A study of histological changes in the Diaphragm of male albino mice administered with aqueous extract of chamomileflowers Chamomillarecutita

2017 ◽  
Vol 14 (3) ◽  
pp. 489-496
Author(s):  
Baghdad Science Journal
Keyword(s):  
Medicinal Plants ◽  
Aqueous Extract ◽  
Muscle Fiber ◽  
Respiratory System ◽  
Respiratory Muscles ◽  
Control Group ◽  
Histological Structure ◽  
Muscular Tissue ◽  
Histological Changes ◽  
Albino Mice

The chamomile is one of the most important medicinal plants recommended for treatment of asthma and some respiratory system diseases. This research was designed to research the effects of aqueous extract of chamomillarecutita on histological structure of Diaphragm of albino mice. The study included 40 male albino mice Musmusculus, their age ranged from (5-7) weeks.The mices were divided randomly to 5 groups and oral administered with 1 ml every day for 10 days:- First Group G1: consider as control group and treated with normal saline,Second Group G2: was treated with aqueous extract of chamomile with concentration of 3 gm /100 ml D.W, Third Group G3: was treated with aqueous extract of chamomile with concentration of 5 gm /100 ml D.W.Fourth Group G4: was treated with aqueous extract of chamomile with concentration of 7 gm /100 ml D.W and the Fifth Group G5: was treated with aqueous extract of chamomile with concentration of 10 gm /100 ml D.W. Theresults of microscopic examination of diaphragm sections of groups G3,G4 and G5 showed degenerative effects on muscular tissue in way of breaking of myofibrils differences in their sizes and degeneration of most of nuclei of muscle fiber and their migration to inside the muscle fiber , it has been found that these treatments cause an alteration in myofibril in fibrotic myofibril. From this study we conclude that low concentration of aqueous extract of chamomile have low side effect on major respiratory muscles and could be used in beneficial treatment to contact diseases of respiratory system but without longer duration.

HISTOLOGICAL CHANGES IN THE INTESTINES OF POULTRY DURING FODDER INTOXICATION

2020 ◽  
Author(s):  
E.P. Dolgov ◽  
◽  
A.A. Abramov ◽  
E.V. Kuzminova ◽  
E.V. Rogaleva ◽  
...  
Keyword(s):  
Body Weight ◽  
Histological Examination ◽  
Aflatoxin B1 ◽  
Structural Changes ◽  
Clinical Manifestations ◽  
Feed Additives ◽  
The Body ◽  
Histological Changes ◽  
Beet Pulp

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.

Histological examination of vascular damage caused by stent retriever thrombectomy devices

2015 ◽  
Vol 8 (10) ◽  
pp. 992-995 ◽  
Author(s):  
Daisuke Arai ◽  
Akira Ishii ◽  
Hideo Chihara ◽  
Hiroyuki Ikeda ◽  
Susumu Miyamoto
Keyword(s):  
Histological Examination ◽  
Carotid Arteries ◽  
Stent Retriever ◽  
Vascular Damage ◽  
Vascular Injuries ◽  
Intimal Thickening ◽  
Histological Changes ◽  
Flow Restoration ◽  
Medial Thickening ◽  
Solitaire Fr

Background and objectivesAlthough the recently marketed stent retriever thrombectomy devices have demonstrated a high recanalization rate and favorable clinical outcomes, there is a concern about the risks of intimal injuries when pulling out the stent in the unfolded position. In this study, the Solitaire Flow Restoration System and the Trevo retriever were used in a histopathological comparison of vascular injuries caused by stent retriever thrombectomy devices.MethodsRabbit carotid arteries were used in the experiments with stent retriever thrombectomy devices. Carotid artery samples were harvested either 1 or 2 weeks postoperatively for histological examination.ResultsHistological changes caused by the use of stent retriever thrombectomy devices were observed from the intimal to medial layers. With the Solitaire FR 4 mm, intimal and medial thickening was observed 1 week postoperatively, and progression of intimal thickening was observed 2 weeks postoperatively. The extent of intimal thickening tended to be greater with the Solitaire FR 6 mm than with the Solitaire FR 4 mm, but this difference was not significant. Compared with the Solitaire FR 4 mm, the Trevo had a significantly smaller area of intimal thickening.ConclusionsAlthough there are some differences among devices, results from this study indicate that stent retriever thrombectomy devices induce vascular damage that extends to the medial layer.

Garcinia Camobogia Extract Alters Anxiety, Sociability, and Dopamine Turnover in Male Swiss Albino Mice

2021 ◽  
Author(s):  
Michael K. Ibrahim ◽  
Marina Aboelsaad ◽  
Fatma Tony ◽  
Moustafa Sayed
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Modern Medicine ◽  
Receptor Expression ◽  
Dopamine Turnover ◽  
Male Mice ◽  
Garcinia Cambogia ◽  
Swiss Albino Mice ◽  
Albino Mice

Abstract Background: Obesity is a global concern, closely allied with somatic and psychosomatic disorders. Herbal drugs are available in modern medicine to treat obesity. Garcinia camobogia being used by so many people trying to lose weight produces various systemic side effects. The study was conducted to assess its effect on anxiety, sociability, and dopamine turnover in male mice. Methodology: Male Swiss albino mice of either were divided into three groups with seven mice in each group. Different groups were given distilled water (0.5ml p.o.) and Garcinia cambogia extract at two different doses (100mg/kg and 500 mg/kg p.o.). Effect of test drugs on anxiety was evaluated using open field test. Sociability and social novelty were evaluated using three chambers test. Results (mean ± standard deviation) were analyzed using one-way ANOVA test followed by Tukey’s test. Result: Garcinia cambogia extract significantly increased the time spent in the corners in the open field test, significantly reduced sociability and social novelty in the three chamber test, significantly reduced dopamine turnover and increased D2 receptor expression in ventral tegmental area. Conclusion: Garcinia cambogia extract have significant anxiogenic effect along with reduced sociability and social novelty in male mice. Moreover, these effects could be related to the altered dopamine turnover and D2 receptor expression in mice brain.

scholarly journals Subchronic Oral Bromocriptine Methanesulfonate Enhances Open Field Novelty-Induced Behavior and Spatial Memory in Male Swiss Albino Mice

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Olakunle James Onaolapo ◽  
Adejoke Yetunde Onaolapo
Keyword(s):  
Spatial Memory ◽  
Open Field ◽  
Cumulative Effect ◽  
Male Mice ◽  
Healthy Male ◽  
Swiss Albino Mice ◽  
Y Maze ◽  
Field Assessment ◽  
Albino Mice ◽  
Neurobehavioral Effects

This study set out to assess the neurobehavioral effects of subchronic, oral bromocriptine methanesulfonate using the open field and the Y-maze in healthy male mice. Sixty adult Swiss albino mice were assigned into three groups. Controls received normal saline, while test groups received bromocriptine methanesulfonate at 2.5 and 5 mg/kg/day, respectively, for a period of 21 days. Neurobehavioral tests were carried out on days 1 and 21 after administration. Open field assessment on day 1 after administration revealed significant increase in grooming at 2.5 and 5 mg/kg, while horizontal and vertical locomotion showed no significant changes. Day 1 also showed no significant changes in Y-maze alternation. On day 21, horizontal locomotion, rearing, and grooming were increased significantly at 2.5 and 5 mg/kg doses after administration; also, spatial memory was significantly enhanced at 2.5 mg/kg. In conclusion, the study demonstrates the ability of oral bromocriptine to affect neurobehavior in normal mice. It also suggests that there is a cumulative effect of oral bromocriptine on the behaviors studied with more changes being seen after subchronic administration rather than after a single oral dose.

scholarly journals Histological changes of uterus after comparative treatment of female rats by Trigonella foenum-graecum seeds and combined oral contraceptive pills

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Ahmed Kaid Naji Allow ◽  
Ayah Rebhi Hilles ◽  
Zainab Yousef ◽  
Norbaiyah Mohamed Bakrim ◽  
Belqees Ahmed ◽  
...  
Keyword(s):  
Body Weight ◽  
Oral Contraceptive ◽  
Treated Group ◽  
Sprague Dawley ◽  
Histological Changes ◽  
Fenugreek Seeds ◽  
Oral Contraceptive Pills ◽  
Trigonella Foenum Graecum ◽  
Combined Oral Contraceptive ◽  
Contraceptive Pills

Introduction: This study evaluates the histological changes of uterine tissue in the female Sprague Dawley rats after administration of Trigonella foenum-graecum (fenugreek) seeds in comparison to combined oral contraceptive pills (COCPs). Methods: Twenty four female Sprague Dawley strain rats of 8 weeks old were divided into A, B, and C groups. Group A was the control and B was administrated with 0.05 mg/kg body weight of COCPs for 15 days. Group C was treated with 750 mg/kg body weight of fenugreek seed aqueous (FSA) extract for 15 days. The uterine tissues were isolated and studied histologically. Results: The uterine weight in the FSA extract treated group was normal, while in the COCPs treated group it was insignificantly increased. The endometrial thickness and endometrial gland density were normal in the FSA extract treated group, while in the COCPs treated group it was atrophied. Decidual-like cells were observed in the COCPs treated group only. Conclusions: Endometrial atrophy and decidual-like cells were noted after COCPs treatment while in the FSA extract and control groups they were absent. The findings suggest that fenugreek seeds could be used as an alternative natural and safe contraceptive product.

scholarly journals Histological Structure of Reproductive Organ of Freshwater Female Crab, Barytelphusa Cunicularis (West-Wood)

2016 ◽  
Vol 5 (3) ◽  
pp. 161 ◽  
Author(s):  
Atul R. Chourpagar ◽  
Rumana Shaikh ◽  
G. K. Kulkarni
Keyword(s):  
Heavy Metals ◽  
Mercuric Chloride ◽  
Sublethal Concentration ◽  
Reproductive Organ ◽  
Histological Structure ◽  
Female Crab ◽  
Histological Changes ◽  
Yolk Granules ◽  
The Mean ◽  
Mean Concentration

<em>Mercury concentrations were recorded in water and tissue of Barytelphusa cunicularis from Pimpalwadi site (Jaikwadi Dam) near Aurangabad. The level of heavy metals in the ovary and spermatheca of crabs was investigated using Atomic Absorption Spectrophotometer (AAS). The mean concentration of mercury in the crab was 0.9 ±0.001 µg/g. A histopathological alteration in ovary and spermatheca was also studied. Several histological changes were noted in the ovary tissue i. e. Distortion of yolk granules, vacuolization, slight necrosis in the oocytes in the ovary and vacuolization observe in lumen, granular substances, sperm mass and spermathecal fluid was evenly distributed in the crab was observed after exposed to sublethal concentration (24<sup>th</sup> of LC<sub>50</sub>:1/5<sup>th</sup> 0.208 ppm) of mercuric chloride.</em>

scholarly journals The Effects of Vasectomy on Testicular Tissue of Mice: Histological Changes and DNA Fragmentation Study

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Salman MO ◽  
Al-Wasiti EA ◽  
Thamir KA ◽  
Al-Ani IM ◽  
Al-Salihi AR
Keyword(s):  
Single Cell ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sham Operation ◽  
Male Mice ◽  
Histological Changes ◽  
Single Cell Gel Electrophoresis

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.

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