scholarly journals Anticancer and Antioxidant Properties of Terpinolene in Rat Brain Cells

2013 ◽  
Vol 64 (3) ◽  
pp. 415-424 ◽  
Author(s):  
Elanur Aydin ◽  
Hasan Türkez ◽  
Şener Taşdemir
Keyword(s):  
Biological Activities ◽  
Antioxidant Properties ◽  
Neuroblastoma Cells ◽  
In Vitro Study ◽  
Cell Types ◽  
Damage Potential ◽  
Total Antioxidant ◽  
Antiproliferative Agent ◽  
Total Oxidative Stress

Abstract Terpinolene (TPO) is a natural monoterpene present in essential oils of many aromatic plant species. Although various biological activities of TPO have been demonstrated, its neurotoxicity has never been explored. In this in vitro study we investigated TPO’s antiproliferative and/or cytotoxic properties using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) test, genotoxic damage potential using the single-cell gel electrophoresis (SCGE), and oxidative effects through total antioxidant capacity (TAC) and total oxidative stress (TOS) in cultured primary rat neurons and N2a neuroblastoma cells. Dose-dependent effects of TPO (at 10 mg L-1, 25 mg L-1, 50 mg L-1, 100 mg L-1, 200 mg L-1, and 400 mg L-1) were tested in both cell types. Significant (P<0.05) decrease in cell proliferation were observed in cultured primary rat neurons starting with the dose of 100 mg L-1 and in N2a neuroblastoma cells starting with 50 mg L-1. TPO was not genotoxic in either cell type. In addition, TPO treatment at 10 mg L-1, 25 mg L-1, and 50 mg L-1 increased TAC in primary rat neurons, but not in N2a cells. However, at concentrations above 50 mg L-1 it increased TOS in both cell types. Our findings clearly demonstrate that TPO is a potent antiproliferative agent for brain tumour cells and may have potential as an anticancer agent, which needs to be further studied.

Antioxidative, anticancer and genotoxic properties of α-pinene on N2a neuroblastoma cells

Biologia ◽  
2013 ◽  
Vol 68 (5) ◽  
Author(s):  
Elanur Aydin ◽  
Hasan Türkez ◽  
Fatime Geyikoğlu
Keyword(s):  
Biological Activities ◽  
Neuroblastoma Cells ◽  
Cell Types ◽  
Mean Values ◽  
N2a Cells ◽  
Diphenyltetrazolium Bromide ◽  
Total Antioxidant ◽  
Total Oxidative Stress ◽  
Oxidative Effects

Abstractα-Pinene, an organic monoterpene, is found in essential oils of pine and coniferous trees. To date, although various biological activities of α-pinene have been demonstrated, its neurotoxicity has never been explored. Therefore in this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties by 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, genotoxic damage potentials by single cell gel electrophoresis, and oxidative effects by total antioxidant capacity (TAC) and total oxidative stress (TOS) analysis of α-pinene. Statistical analysis of MTT assay results indicated significant (p < 0.05) decreases of the cell proliferation rates in healthy neurons treated with α-pinene at only 400 mg/L, while significant decreases were observed in N2a cells at 100, 200 and 400 mg/L. On the other hand, the mean values of the total scores of cells showing DNA damage were not found significantly different from the control values on both cells. In addition, our results indicated that 10 and 25 mg/L of α-pinene treatment caused increases of TAC levels in primary rat neurons without any alterations of its level in N2a cells. However, α-pinene treatments at higher doses led to increases of TOS levels in both cell types. Overall our results suggest that α-pinene is of a limited therapeutic use as an anticancer agent.

scholarly journals Antiproliferative, genotoxic and oxidant activities of cyclosativene in rat neuron and neuroblastoma cell lines

2014 ◽  
Vol 66 (3) ◽  
pp. 1171-1177
Author(s):  
Başak Toğar ◽  
Hasan Türkez ◽  
Fatime Geyikoğlu ◽  
Ahmet Hacimüftüoğlu ◽  
Abdulgani Tatar
Keyword(s):  
Dna Damage ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Cell Types ◽  
Damage Potential ◽  
Mean Values ◽  
Abies Magnifica ◽  
Total Antioxidant ◽  
Total Oxidative Stress

Cyclosativene (CSV) is a tetracyclic sesquiterpene found in the essential oils of Centaurea cineraria (Asteraceae) and Abies magnifica A. Murray (Pinaceae) plants. To the best of our knowledge, its cytotoxic, genotoxic and oxidant effects have never been studied on any cell lines. Therefore, we aimed to investigate the in vitro antiproliferative and/or cytotoxic properties, antioxidant/oxidant activity and genotoxic damage potential of CSV in healthy neurons and N2a neuroblastoma (N2a-NB) cell cultures. After treatment with 10-400 ?g/ml of CSV for 24 h, cell proliferation was measured by the MTT (3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antioxidant activity was assessed by the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. To evaluate the level of DNA damage, single cell gel alkaline electrophoresis (SCGE) was used. The MTT assay showed that the application of CSV significantly reduced cell viability in both cell types. CSV treatments at higher doses led to decreases of TAC levels and increases of TOS levels in neuron and N2a-NB cells. The mean values of the total scores of cells showing DNA damage were not found to be significantly different from the control values in both cells. In conclusion, this study suggests that CSV has weak anticancer potential.

scholarly journals The in vitro cytotoxicity, genotoxicity and oxidative damage potential of dapagliflozin, on cultured human blood cells

2019 ◽  
Vol 44 (5) ◽  
pp. 692-698
Author(s):  
Kenan Çadırcı ◽  
Özlem Özdemir Tozlu ◽  
Hasan Türkez
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
In Vitro Cytotoxicity ◽  
Damage Potential ◽  
Human Blood Cells ◽  
Diphenyltetrazolium Bromide ◽  
Total Antioxidant ◽  
Release Assay ◽  
Total Oxidative Stress

Abstract Objectives Dapagliflozin (DAPA), is a potent SGLT-2 inhibitor for the treatment of patients with type 2 diabetes. DAPA has a good clinical and biological tolerance profile. However little information is available on its potential effects on cultured human blood cells. The evaluation of the in vitro cytotoxicity, genotoxicity potential and antioxidant/oxidant activity of DAPA in primary human whole blood cell cultures was aimed in this study. Materials and methods Cell viability was measured by the MTT [3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and lactate dehydrogenase (LDH) leakage assays. The antioxidant/oxidant activity was determined by measuring the total antioxidant capacity (TAC) and total oxidative stress (TOS) levels. To assess the genotoxicity of DAPA, chromosomal aberration (CA) frequencies were determined. Results MTT and LDH release assay exhibited that exposure to different doses of DAPA did not changed significantly the proliferation of cells. The results of TAC and TOS assays were showed that TAC level was elevated while TOS level did not altered in DAPA-treated cells. Moreover, any increase in the frequency of CA did not found on cultures blood cells. Conclusion These data indicate that DAPA has not cytotoxic and genotoxic potential in cultured human blood cells, also, induces the increasing antioxidant activity.

scholarly journals Protective, Antioxidant and Antiproliferative Activity of Grapefruit IntegroPectin on SH-SY5Y Cells

2021 ◽  
Vol 22 (17) ◽  
pp. 9368
Author(s):  
Domenico Nuzzo ◽  
Miriana Scordino ◽  
Antonino Scurria ◽  
Costanza Giardina ◽  
Francesco Giordano ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Neuroblastoma Cells ◽  
Therapeutic Strategies ◽  
Brain Disorders ◽  
Citrus Pectin ◽  
Linalool Oxide ◽  
Antiproliferative Agent ◽  
High Doses ◽  
Inhibit Cell Proliferation

Tested in vitro on SH-SY5Y neuroblastoma cells, grapefruit IntegroPectin is a powerful protective, antioxidant and antiproliferative agent. The strong antioxidant properties of this new citrus pectin, and its ability to preserve mitochondrial membrane potential and morphology, severely impaired in neurodegenerative disorders, make it an attractive therapeutic and preventive agent for the treatment of oxidative stress-associated brain disorders. Similarly, the ability of this pectic polymer rich in RG-I regions, as well as in naringin, linalool, linalool oxide and limonene adsorbed at the outer surface, to inhibit cell proliferation or even kill, at high doses, neoplastic cells may have opened up new therapeutic strategies in cancer research. In order to take full advantage of its vast therapeutic and preventive potential, detailed studies of the molecular mechanism involved in the antiproliferative and neuroprotective of this IntegroPectin are urgently needed.

scholarly journals Neuroprotective, antioxidant and antiproliferative activity of grapefruit IntegroPectin on SH-SY5Y cells

2021 ◽  
Author(s):  
Domenico Nuzzo ◽  
Miriana Scodino ◽  
Antonino Scurria ◽  
Costanza Giardina ◽  
Francesco Giordano ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Neuroblastoma Cells ◽  
Health And Safety ◽  
Grape Fruit ◽  
Linalool Oxide ◽  
Excellent Health ◽  
Antiproliferative Agent ◽  
High Doses ◽  
Inhibit Cell Proliferation

Tested in vitro on SH-SY5Y neuroblastoma cells, grapefruit IntegroPectin is a powerful neuro-protective, antioxidant and antiproliferative agent. The strong antioxidant properties of grape-fruit IntegroPectin, and its ability to preserve mitochondrial membrane potential and mor-phology, severely impaired in neurodegenerative disorders, make this new biopolymer highly soluble in water an attractive therapeutic agent for oxidative stress-associated brain disorders. Similarly, the ability of this new citrus pectin rich in naringin, linalool, linalool oxide and lim-onene adsorbed at the outer surface to inhibit cell proliferation or even kill, at high doses, neo-plastic cells, coupled to its excellent health and safety profile, opens up new therapeutic strate-gies in cancer research. In order to take full advantage of its vast therapeutic and preventive po-tential, detailed studies of molecular mechanism involved in the antiproliferative and neuro-protective of IntegroPectin are urgently needed.

scholarly journals Assessment of the Mutagenic Activity of Extracts of Brazilian Propolis in Topical Pharmaceutical Formulations on Mammalian CellsIn VitroandIn Vivo

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Juliana Marques Senedese ◽  
Aline Rafaela Rodrigues ◽  
Michelle Andrade Furtado ◽  
Viviane Dias Faustino ◽  
Andresa A. Berretta ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Mutagenic Activity ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Test System ◽  
Negative Control ◽  
Continuous Treatment ◽  
Mutagenic Potential ◽  
Topical Formulations

Propolis possesses various biological activities such as antibacterial, antifungal, anti-inflammatory, anesthetic and antioxidant properties. A topically applied product based on Brazilian green propolis was developed for the treatment of burns. For such substance to be used more safely in future clinical applications, the present study evaluated the mutagenic potential of topical formulations supplemented with green propolis extract (1.2, 2.4 and 3.6%) based on the analysis of chromosomal aberrations and of micronuclei. In thein vitrostudies, 3-h pulse (G1phase of the cell cycle) and continuous (20 h) treatments were performed. In thein vivoassessment, the animals were injured on the back and then submitted to acute (24 h), subacute (7 days) and subchronic (30 days) treatments consisting of daily dermal applications of gels containing different concentrations of propolis. Similar frequencies of chromosomal aberrations were observed for cultures submitted to 3-h pulse and continuous treatment with gels containing different propolis concentrations and cultures not submitted to any treatment. However, in the continuous treatment cultures treated with the 3.6% propolis gel presented significantly lower mitotic indices than the negative control. No statistically significant differences in the frequencies of micronuclei were observed between animals treated with gels containing different concentrations of propolis and the negative control for the three treatment times. Under the present conditions, topical formulations containing different concentrations of green propolis used for the treatment of burns showed no mutagenic effect in either test system, but 3.6% propolis gel was found to be cytotoxic in thein vitrotest.

Human umbilical cord blood-derived cells differentiate into hepatocyte-like cells in the Fas-mediated liver injury model

2005 ◽  
Vol 289 (6) ◽  
pp. G1091-G1099 ◽  
Author(s):  
Kazunobu Nonome ◽  
Xiao-Kang Li ◽  
Terumi Takahara ◽  
Yusuke Kitazawa ◽  
Naoko Funeshima ◽  
...  
Keyword(s):  
Liver Injury ◽  
Umbilical Cord Blood ◽  
Fas Ligand ◽  
In Vitro Study ◽  
Cell Types ◽  
Human Umbilical Cord Blood ◽  
Specific Gene ◽  
Human Umbilical Cord

Human umbilical cord blood (HUCB) contains stem/progenitor cells, which can differentiate into a variety of cell types. In this study, we investigated whether HUCB cells differentiate into hepatocytes in vitro and in vivo. We also examined whether CD34 could be the selection marker of stem cells for hepatocytes. HUCB cells were obtained from normal full-term deliveries, and CD34+/−cells were further separated. For in vitro study, HUCB cells were cultured for 4 wk, and expressions of liver-specific genes were examined. For the in vivo study, nonobese diabetic/severe combined immunodeficient mice were subjected to liver injury by a Fas ligand-carried adenoviral vector or only radiated. Mice were treated simultaneously with or without cell transplantation of HUCB, CD34+, or CD34−cells. After 4 wk, human-specific gene/protein expression was examined. In the in vitro study, human liver-specific genes were positive after 7 days of culture. The immunofluorescent study showed positive staining of α-fetoprotein, cytokeratin 19, and albumin in round-shaped cells. In the in vivo study, immunohistochemical analysis showed human albumin-positive, hepatocyte-specific antigen-positive cells in mouse livers of the Fas ligand/transplantation group. Fluorescence in situ hybridization analysis using the human Y chromosome also showed positive signals. However, no difference between transplanted cell types was detected. In contrast, immunopositive cells were not detected in the irradiated/transplantation group. The RT-PCR result also showed human hepatocyte-specific gene expressions only in the Fas ligand/transplantation group. HUCB cells differentiated into hepatocyte-like cells in the mouse liver, and liver injury was essential during this process. The differences between CD34+and CD34−cells were not observed in human hepatocyte-specific expression.

scholarly journals In Vitro study for hesperidin nanoparticles effect on phagocytic activity against Staphylococcus aureus

2018 ◽  
Vol 12 (2) ◽  
pp. 36-39
Author(s):  
Saja Ali ◽  
Ghassan Sulaiman ◽  
Mohammed M. Al-Halbosiy
Keyword(s):  
Biological Activities ◽  
In Vitro Study ◽  
Particle Size Analysis ◽  
Poloxamer 407 ◽  
Size Analysis ◽  
Therapeutic Effects ◽  
X Ray Diffraction ◽  
Setup Cost ◽  
Phagocytosis Index

       Hesperidin is one of the flavonoids from citrus peels and it recognized to possess various biological activities such as, anti-inflammatory, anti-carcinogenic, antioxidant and antimicrobial potentials. The present investigation studies the immunological adjuvant influence of hesperidin nanoparticles. Hesperidin nanoparticles were prepared by nano-precipitation technique by using Poly (D, L-lactic-co-glycolic acid) (PLGA) polymer and Poloxamer 407 was used as a stabilizer. This method was used because of their advantage of low setup cost and simplicity. Hesperidin nanoparticles were characterized by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and particle size analysis (PSA) analytical methods. The effect of hesperidin nanoparticles was higher than the effect of pure hesperidin, and there was an obvious increase in phagocytosis index (PI 82%) of hesperidin nanoparticles when compared with pure hesperidin (PI 56%) and in comparison with the control samples (PI 22%). In conclusion we need further studies about if nano-hesperidin has therapeutic effects.

In Vitro Study of Antioxidant Activity of Serum and Plasma Samples As Well As Glutathione Exposed to Various Exogenous Stress Factors

2021 ◽  
Author(s):  
Małgorzata Olszowy-Tomczyk ◽  
Łukasz Paprotny ◽  
Agnieszka Celejewska ◽  
Dorota Szewczak ◽  
Dorota Wianowska
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
In Vitro Study ◽  
Stress Factors ◽  
Plasma Samples ◽  
Serum Samples ◽  
Exogenous Stress

Abstract The imbalance between the production of Reactive Oxygen Species (ROS) and their sequestration promotes the formation of so-called oxidative stress conditions which are considered crucial in the aging process and development of many human diseases. Glutathione plays an essential role in the antioxidative barricade against ROS. Its role in the detoxification process of xenobiotics and carcinogen is also known. However, there are no comparative studies on the antioxidant properties of both biological samples and glutathione as well as the change in these properties as a result of exposure to various stress factors. This paper fills this gap comparing the antioxidant activity of serum and plasma samples of the known glutathione content with the activity of glutathione itself assessed by the different methods. In addition, it reveals a significant role of environmental xenobiotics in oxidative stress and differentiates the stress induced by different groups of drugs, among which the greatest one has been demonstrated for antiarrhythmic drugs and cytostatics. More importantly, it proves that human plasma is more resistant to stress factors and N-acetylcysteine clearly promotes the extension of antioxidant properties of both the plasma and serum samples. The latter conclusion is consistent with the implied preventive and/or supportive action of this drug against SARS-CoV-2.

scholarly journals IN VITRO STUDY OF ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF SOME MEDICINAL PLANTS AND THEIR INTERRELATIONSHIP

2018 ◽  
Vol 11 (4) ◽  
pp. 195 ◽  
Author(s):  
Shubhi Rastogi ◽  
Mohammed Shariq Iqbal ◽  
Deepak Ohri
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Cannabis Sativa ◽  
Methanolic Extract ◽  
In Vitro Study ◽  
Strong Positive Correlation ◽  
Total Antioxidant ◽  
Anti Inflammatory

 Objective: The objective of the present work is to study the in vitro anti-inflammatory and antioxidant activity of medicinal plants. The extent and correlation between anti-inflammatory and antioxidant activity have been studied.Method: Methanolic and aqueous extracts of five medicinal plants, namely, Ficus racemosa, Aloe vera, Cannabis sativa, Datura stramonium, and Calotropis gigantean have been taken for in vitro anti-inflammatory and total antioxidant activity.Result: The study showed that the inhibition of protein (albumin) denaturation was maximum in aqueous extract of A. vera with 97.55±1.45%. Proteinase inhibitory action of different plant extracts showed significant action and was found to be maximum in aqueous extract of D. stramonium with 87.89±2.58%. Heat-induced hemolysis showed that maximum inhibition was with aqueous extract of F. racemosa with 90.72±3.33%. When hypotonicity-induced hemolysis activity was done it was found maximum in methanolic extract of C. gigantea with 90.58±3.04%. Anti-lipoxygenase activity was found maximum in methanolic extract of F. racemosa with 94.05±4.24%. When total antioxidant activity was done, it was found highest in F. racemosa (4.38±0.546 mM equivalent of ascorbic acid/g tissue).Conclusion: An overall strong positive correlation between anti-inflammatory and antioxidant activity was observed, indicating that antioxidant activity of the plant species studied might be responsible for their anti-inflammatory property. Further work needs to be undertaken to fully elucidate the antioxidants responsible for anti-inflammatory action and to develop better herbal drug formulations.

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