scholarly journals Application of high hydrostatic pressure (HHP) to improve cryopreservation of young bull semen

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Barbara Szczęśniak-Fabiańczyk ◽  
Piotr Gogol ◽  
Lechosław Gajda ◽  
Zdzisław Smorąg
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Computer Assisted ◽  
Motile Sperm ◽  
Cell Membrane Integrity ◽  
Progressive Motility ◽  
Bull Semen

Abstract The objective of the study was to determine the effect of high hydrostatic pressure (HHP) on quality of cryopreserved semen of young bulls. Semen for this study was collected from 8 bulls aged between 13 and 18 months at monthly intervals, from June to September. After collection, semen was diluted in a commercial Bioxcell® extender (one part at 1:1 and a second part to give a sperm concentration of 20 million/0.2 mL), filled into straws and treated with HHP at 30 MPa for 90 min. After HHP treatment, pre-diluted semen (1:1) was diluted to a sperm concentration 20 million/0.2 mL and filled into straws. In addition, part of the semen diluted to a concentration of 20 million/0.2 mL was not treated with HHP (control). All of it was held at +4°C and frozen in a freezer after 2.5-h equilibration. Semen was thawed in a water bath at 38°C and subjected to estimation of the percentage of motile sperm both subjectively and using a computer-assisted semen analyzer and cytometric assessment of sperm cell membrane integrity. Subjective motility and fast progressive motility were significantly higher with pre-diluted (1:1) and HHP treated semen compared to control (P<0.05). No significant differences were observed in percentage of membrane-intact spermatozoa between control and experimental groups. Additionally, the influence of HHP on the sperm of individual bulls was assessed. In bull number 2, the HHP treatment after semen pre-dilution significantly improved progressive motility from 54.1 to 63.4 percent (P <0.05). In bull number 4, the HHP treatment after semen pre-dilution significantly improved subjective motility, rapid motility and progressive motility by 12.5, 16.8 and 16.3 percent, respectively (P<0.05). No effect was seen for 6 bulls. It is concluded that for some bulls, the application of HHP before semen freezing may improve the cryopreservation outcome. However, this requires further research in this area, also to determine the fertilizing capacity of bull semen exposed to high hydrostatic pressure.

scholarly journals Assessment of quality in specific fractions of Large White Yorkshire boar semen

2021 ◽  
Vol 52 (2) ◽  
Author(s):  
K. G. Ambily ◽  
Malati Naik ◽  
Hiron M. Harshan ◽  
C. Jayakumar ◽  
M. P. Unnikrishnan ◽  
...  
Keyword(s):  
Cold Shock ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Membrane Cholesterol ◽  
Large White ◽  
Progressive Motility ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Quality Assessments

Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.

scholarly journals Effect of N-Methylacetamide Concentration and Thawing Rate on Chicken Sperm Quality after Cryopreservation

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 824
Author(s):  
Fabio Mosca ◽  
Luisa Zaniboni ◽  
Ahmad Abdel Sayed ◽  
Nicolaia Iaffaldano ◽  
Dominga Soglia ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Kinetic Parameters ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Motile Sperm ◽  
Cell Membrane Integrity ◽  
Before And After ◽  
Freezing Procedure ◽  
Thawing Rate

In seeking alternative cryoprotectants to glycerol for a reference chicken semen freezing procedure, the aim of the present study was to compare the effect of two concentrations of N-Methylacetamide (MA) and two thawing rates on the quality of frozen-thawed semen. Semen samples were diluted in Lake pre-freezing extender, including 0.1 M trehalose in presence of 6% or 9% MA, loaded into straws, frozen in nitrogen vapors, and stored in liquid nitrogen. The following thawing treatments were used: 5 °C for 100 s and 38 °C for 30 s. Sperm quality (cell membrane integrity, motility and kinetic parameters) was assessed before and after cryopreservation. The decrease of MA concentration from 9 to 6% improved sperm quality after freezing/thawing and this effect was dependent on thawing temperature. Decreasing the MA concentration from 9 to 6% improved the proportion of undamaged membrane, motile, and progressive motile sperm recovered after thawing at 5 °C for 100 s; in contrast, no effect of the MA concentration was observed thawing at 38 °C for 30 s. Therefore, the treatment with 6% MA and thawing at 5 °C for 100 s has given the best cryoprotective action. These results contribute to improve the efficacy of the current chicken semen cryopreservation procedures.

scholarly journals The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition

2020 ◽  
Vol 45 (3) ◽  
pp. 197-205
Author(s):  
I. Darussalam ◽  
R. I. Arifiantini ◽  
I. Supriatna ◽  
R. S. D. Rasad
Keyword(s):  
Sperm Concentration ◽  
Egg Yolk ◽  
Carnitine Supplementation ◽  
Equal Part ◽  
Liquid Form ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Bull Semen ◽  
Sperm Abnormalities

The study was conducted to investigatethe effect of L-Carnitine supplementation in Tris-eggyolk (TEY) diluents to optimize the quality of Pasundan bull liquid semen. Semen samples were collected from three Pasundan bulls (6 – 7 years old)usinganartificial vagina. Semen samples were evaluated macroscopically and microscopically, semen having ≥70% progressive motility, ≥500×106ml-1 sperm concentration and ≤ 20% sperm abnormalities were divided into 5 equal part. Each part  wasdiluted with TEYand supplemented with0 mM(control), 1 mM, 2 mM, 3 mM and 4 mM L-Carnitine. Liquidsemen was storedat5°C.Sperm motility was evaluated every 12 h  objectively using Computer Asissted Sperm Analysis (CASA) until progressive motilityreached 40%. The best diluent was demonstratedby progressive motility (PM)by TEY supplemented with 1 mM L-Carnitine(43.08±0.49%) that remained up to 108 h compared tothecontrol group (43.63±0.70%) that remained up to 72 h (P<0.05). In conclusion, 1 mM L-Carnitine in TEY was the best concentration for preservation of Pasundan bull semen stored in the liquid form. 

128 IMPROVED POST-THAW MOTILITY, VIABILITY, AND FERTILITY ARE ACHIEVED BY HYDROSTATIC PRESSURE-TREATED BULL SEMEN

2007 ◽  
Vol 19 (1) ◽  
pp. 181 ◽  
Author(s):  
C. Pribenszky ◽  
M. Molnár ◽  
A. Horváth ◽  
G. Kútvölgyi ◽  
A. Harnos ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Confidence Interval ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
T Test ◽  
Return Rate ◽  
Bull Semen ◽  
Frozen Semen ◽  
Paired T Test ◽  
Analyze Data

Previously, we reported that high hydrostatic pressure (HHP) significantly improves post-thaw survival of frozen mouse and IVP bovine blastocysts, presumably from the induction of shock proteins (Pribenszky et al. 2005 Anim. Repr. Sci. 87, 143–150; 2005 Repr. Dom. Anim. 40, 338). We also have reported increased post-thaw survival of HHP-treated boar and bull semen (Pribenszky et al. 2004 Repr. Fert. Dev. 17, 199–200; 2005 Repr. Fert. Dev. 18, 162–163). We now report further data on the effect of HHP treatment on motility, viability, and fertility of frozen–thawed bull semen. HHP treatments were executed by a computer-controlled pressurizing device (Cryo-Innovation, Ltd., Budapest, Hungary). Semen of 21 bulls was diluted individually to a sperm concentration of 8 � 107 mL-1 with AndroMed� or Triladyl� extenders (MiniT�b, Tiefenbach, Germany). Diluted sperm was loaded into 0.25-mL straws at 25�C, and then divided into 2 groups: one group was treated with a HHP pulse defined and optimized earlier (30 MPa for 90 min); the other group was held at 5�C for the corresponding time. After HHP, treated samples also were placed at 5�C for 3–4 h. After equilibration at 5�C, samples were frozen (10 min at -110�C, and then plunged into LN2). Straws were thawed in a 35�C water bath for 30 s. Progressive motility was assessed by the CASA system (MiniT�b). Experiments were replicated twice for each bull. Paired t-test was used to analyze data. HHP treatment significantly increased the post-thaw motility of the frozen semen of the bull population examined. The mean of the differences was 21.14% (95% confidence interval: 13.56–28.72); P = 1.08 � 10-5. The post-thaw motility of 3, 7, and 5 of the 21 bulls increased by an additional 35–60%, 25–35%, and 10–25%, respectively; no effect was seen for 6 of the bulls. Four of the 7 bulls with low (3–19%) post-thaw motility were improved to the range of 43–70% by HHP treatment. Semen of 10 bulls was the subject of viability analysis individually. Sperm head, tail, and acrosome membrane integrity were evaluated with Kovacs-Foote staining (Kovacs and Foote 1992 Biotech. Histochem. 67, 119–124) and counting 300 sperm/sample. Paired t-test was used to analyze data. With HHP treatment, the proportion of the cells with intact tail, head, and acrosome increased significantly (P d 0.008). Eighty-two cows were inseminated with HHP-treated frozen–thawed semen; the 60 days non-return rate was 90.24%, whereas the 60 days non-return rate in the same population and time without treatment was 82.3% (n = 4789). Data were analyzed with the exact binomial test. HHP treatment significantly improved the non-return rate (P = 0.035; 95% confidence interval: 0.83–1.00). HHP treatment substantially increases the post-thaw semen quality of the bull population. Also, the semen of a proportion of bulls with very low semen freezability can be increased to the range where it can be frozen commercially. Further investigations are needed, including large-scale field trials incorporating the insemination of the otherwise low freezers and the biological background of the process. This work was supported by GVOP-TST050157 and Besamungsanstalt Klessheim.

Evaluate the fresh and Post Thawing Quality of Holstein Friesian Bull semen

2019 ◽  
Vol 1 (1) ◽  
pp. 22-26
Author(s):  
Jamal Mehmood shah ◽  
Tahir Hameed ◽  
Farhat abbas Bokhari ◽  
Gul zaman
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Pooled Data ◽  
Bull Semen ◽  
Holstein Friesian ◽  
Semen Volume ◽  
Sperm Membrane ◽  
Mass Activity

TThe   study   was  carried   out   during the yearr  2017   to   determine   the   fresh   and   post-thaw   quality   of   four   Holstein   Friesian   bulls.   Semen   samples   were   examined   for ejaculate   volume,   pH,   mass   activity,   sperm   concentration   and   sperm   motility and   sperm   membrane   integrity   (HOST).   The   ejaculated   semen   volume   of   Holstein   Friesian   bulls   was   in   the   range   of   6-8ml   and   differences   in   ejaculates   were   significant   (P<0.01).   The   volume   was   significantly   (P<0.05)   higher   (7.75ml)   when   collected   on   5th   July,   slightly   decrease   in   volume   (7.25ml)   when   collected   on   12th   May   and   26th   July.   Semen   pH   was   higher   (6.65)   for   26th   July   ejaculation   and   lowest (6.55ml)   for   24th   May   ejaculation.   The   results   indicated   that   the   semen   of   Holstein   Friesian   bulls   did   not   have   considerable   variation   in   pH   during   May   –   July.   Most   of   the   semen   samples   were   creamy   white and   yellow   in   colour,   while   few   samples   were   milky   white   and   watery.   Mass   activity   score   of   semen   samples   indicated   vigorous   movement with   moderate rapid   waves   and   eddies.   The   sperm   motility   of   fresh   vs   post-thaw   semen   was   obtained   72.80   vs   48.75   percent   (12th   May),   75.00   vs   48.75   percent   (24th   May),   76.25   vs   55.00   percent   (2nd   June),   73.75   vs   52.50   percent   (21st   June),   75.00   vs   56.25   (5th   July)   and   75.00   vs   50.00   percent   (26th   July).   In   case   of   post-thaw   semen,   highest   sperm   motility   of   56.25   percent   was   recorded   in   semen   ejaculated   on   5th   July,   while   lowest   post-thaw   sperm   motility   of   48.75   percent   was   observed   in   semen   ejaculated   on   12th   May   and   24th   May.   Highest   sperm   concentration   of   1549.75x106   was   determined   in   semen   ejaculated   on   21.06.2017   and   lowest   concentration   (1259.50x106)   in   26.07.2017   collected   samples.   It   was   concluded   that   sperm   motility   was   significantly   (P<0.01)   affected   by   semen   types   (fresh   and   post-thaw),   while   sperm   concentration   was   also   significantly   (P<0.01)   affected   by   ejaculation   date   and   bulls.   The   membrane   integrity   (HOST)   of   the   pooled   data   on   fresh   semen   samples   over   a   period   of   three   months   was   58.37   percent   against   post-thaw   membrane   integrity   of   44.79   percent.

scholarly journals 98 THE EFFECT OF HIGH HYDROSTATIC PRESSURE ON THE MOTILITY OF FRESH AND FROZEN-THAWED BULL SEMEN

2005 ◽  
Vol 17 (2) ◽  
pp. 199 ◽  
Author(s):  
C.S. Pribenszky ◽  
M. Molnar ◽  
L. Solti ◽  
J. Dengg ◽  
J. Lederer
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Survival Rates ◽  
Sperm Concentration ◽  
Control Group ◽  
Pressure Treatment ◽  
Treatment Groups ◽  
Bull Semen ◽  
Pressure Time ◽  
Pre Treatment

Previously, we reported that a sublethal shock, high hydrostatic pressure (HHP), significantly improves the post-thaw survival of frozen mouse blastocysts, presumably from the induction of shock proteins (Pribenszky et al. 2004 Reprod. Fert. Dev. 16, 181). Others reported that HSP90 in spermatozoa decreased substantially after freezing (Huang et al. Theriogenology 51, 1007–1016; Cao Wen-Lei et al. 2003 Asian J. Androl. 5, 43–46). We now report the effect of HHP on motility of the fresh bull semen to determine whether sperm survives in an altered pressure environment, and to compare post-thaw motility of HHP-treated frozen bull semen with controls. The survival rates were compared by chi-square test. Expt 1: Semen of one bull was diluted to a sperm concentration of 8 × 107/mL with AndroMed extender (MiniTüb, Tiefenbach, Germany). Diluted sperm was loaded into 0.25-mL straws at 25°C. Each straw was cut in half. One demi-straw was heat-sealed and exposed to HHP, and sperm in the companion demi-straw served as a control. Experiments were replicated eight times for each pressure/time treatment. Progressive motility was assessed independently by light microscopic investigation by two individuals. The treatment groups were: 10 MPa for 30, 60, 90, or 120 min; 30 MPa for 30, 60, 90, 120, or 510 min; 50 MPa for 30, 60, or 90 min; 70 MPa for 30, 60, or 90 min; and 90 MPa for 30, 60, 90, 120, or 510 min. The average motility of the control samples ranged from 75 to 90%, while the average motility of the pressurized samples ranged between 55 (90 MPa/120 min) to 84% (10 MPa/30 min). The groups of 30 MPa/510 min and 90 MPa/510 min exhibited significantly lower motility compared to the other pressurized groups (27% and 33%, respectively; P < 0.05). Expt 2: Semen was collected from two bulls with poor sperm freezability. Semen was diluted as described for the first experiment, loaded into straws, and assigned to one of 4 treatment groups. Half the straws from each bull were exposed to 90 MPa/30 min, 90 MPa/90 min, 30 MPa/30 min, or 30 MPa/90 min, and then cryopreserved. Controls consisted of straws that were cryopreserved without pressure treatment. Cryopreservation steps were 60 min equilibration at 5°C, followed by 10 min at −110°C, and then plunging into liquid nitrogen. Straws were thawed in a 35°C water-bath for 30 s. Each treatment and control group was replicated 8 times (8 samples per bull). The average post-thaw motility was significantly superior with pressure pre-treatment in each of the pressurized groups compared to the samples frozen without previous pressurization (P < 0.001) (Bull I: 2–3% without pressurization vs. 17–33% with pressurization; Bull II: 0% without pressurization vs. 21–35% with pressure pre-treatment). Among the pressure/time parameters used, 30 MPa/90 min proved significantly superior (33 and 35%; P < 0.05) for each of the bulls. Expt. 2 clearly demonstrates the beneficial effect of a previous pressure treatment on post-thaw motility of bull semen cryopreserved in our experiment. Further investigations are needed, including samples from different bulls, different freezing protocols, and the biological background of the process. This work was supported partly by NKFP 4/040/2001.

scholarly journals Semen quality of Colombian Creole as compared to commercial pig breeds

2020 ◽  
Author(s):  
Rafael Suárez Mesa ◽  
Joan Estany ◽  
Iang Schroniltgen Rondón-Barragán
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Phenotypic Characterization ◽  
Computer Assisted ◽  
Motile Sperm ◽  
Sperm Analysis ◽  
Rural Livelihood ◽  
Computer Assisted Sperm Analysis

Abstract Background Colombian Creole pigs are a valuable resource for rural livelihood and research. There are three officially recognized Creole breeds in Colombia (Zungo, ZU; Casco de Mula, CM; and San Pedreño, SP). The phenotypic characterization of these breeds is still very limited, including the reproductive performance of their boars, which is a key issue for developing conservation and dissemination strategies. The aim of this research was to assess the semen quality of Colombian Creole breeds as compared to commonly used international breeds. Results Seven boars for each Colombian Creole (ZU, CM, and SP) and international (Duroc, Belgian Landrace, and Pietrain) breeds were used in the experiment. Two doses of semen per boar were assessed in duplicate for sperm kinetics and membrane and acrosome integrity using computer-assisted sperm analysis and flow cytometry, respectively. On average, the Creole pigs, as compared to international breeds, showed lower (P<0.05) volumeof fluid ejaculated(185.5 mL vs 239.9 mL) as well as sperm concentration (340.5 vs to 395.4, in million sperm/mL), motility (90.9% vs 95.3%) and progressive motility (63.1% vs 67.2%). No relevant differences between breeds for sperm velocity traits were observed, but Creole pigs had lower (P<0.05) proportion of morphologic normal sperm (86.1% vs 90.6%) and of sperm with intact mitochondria plasma membrane and acrosome (76.8% vs 87.5%). Mitochondrial membrane potential did not differ between Creole and international breeds. These results mean that Creole breeds had 60.5% less normal and motile sperm per ejaculate than international breeds. Amongst Creole breeds, SP had larger ejaculates and ZU showed greater proportion of normal and motile sperm, but they did not differ for the amount of normal and motile sperm per ejaculate. Conclusion The semen of Colombian Creole pigs is acceptable but less abundant and rich in normal and motile spermatozoa than that collected from commercial breeds. This fact should be considered in developing recommendations for semen processing in Creole pigs. Findings provided here can give new impetus to the conservation and insemination of Creole pigs.

Effect of docosahexanoic acid on quality of frozen–thawed bull semen in BioXcell extender

2017 ◽  
Vol 29 (3) ◽  
pp. 490 ◽  
Author(s):  
Asmatullah Kaka ◽  
Wahid Haron ◽  
Rosnina Yusoff ◽  
Nurhusien Yimer ◽  
A. M. Khumran ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Membrane Integrity ◽  
Optimum Level ◽  
Docosahexanoic Acid ◽  
Normal Morphology ◽  
Bull Semen ◽  
Acrosome Integrity

This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen–thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extended into BioXcell extender to which 0 (control), 3, 5, 10 or 15 ng mL–1 DHA was added. The supplemented semen samples were incubated at 37°C for 15 min for DHA uptake by spermatozoa. Later, samples were cooled for 2 h at 5°C and packaged into 0.25-mL straws, frozen in liquid nitrogen for 24 h and subsequently thawed for evaluation. Results are presented as percentages ± s.e.m. Supplementation with DHA at 3 ng mL–1 significantly improved sperm functional parameters including sperm motility, normal morphology, viability, acrosome integrity and membrane integrity when compared with other supplemented groups and the control. Lipid peroxidation increased as the incorporation of DHA supplementation increased. In conclusion, 3 ng mL–1 concentration of DHA resulted in superior quality of frozen–thawed bull spermatozoa and is suggested as the optimum level of DHA to be added into BioXcell extender.

scholarly journals Effects of High Hydrostatic Pressure on the Conformational Structure and Gel Properties of Myofibrillar Protein and Meat Quality: A Review

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1872
Author(s):  
Huipeng Liu ◽  
Yiyuan Xu ◽  
Shuyu Zu ◽  
Xuee Wu ◽  
Aimin Shi ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Tertiary Structure ◽  
Quaternary Structure ◽  
Meat Products ◽  
Myofibrillar Protein ◽  
Conformational Structure ◽  
Gel Properties ◽  
The Relationship

In meat processing, changes in the myofibrillar protein (MP) structure can affect the quality of meat products. High hydrostatic pressure (HHP) has been widely utilized to change the conformational structure (secondary, tertiary and quaternary structure) of MP so as to improve the quality of meat products. However, a systematic summary of the relationship between the conformational structure (secondary and tertiary structure) changes in MP, gel properties and product quality under HHP is lacking. Hence, this review provides a comprehensive summary of the changes in the conformational structure and gel properties of MP under HHP and discusses the mechanism based on previous studies and recent progress. The relationship between the spatial structure of MP and meat texture under HHP is also explored. Finally, we discuss considerations regarding ways to make HHP an effective strategy in future meat manufacturing.

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