Immune Response and Bone Marker Enzyme Activities of Broiler Birds Fed Graded Level Taurine-Supplemented-Diets

Abstract Taurine, a derived amino acid has been proven to play an important biological roles in enhancing bone strength and immune activities of broiler birds. This research investigated the effect of the different concentrations of graded level dietary taurine supplement on immune response of birds against Newcastle Disease Virus (NCDV) and Infectious Bursal Disease Virus (IBDV), as well as on enzymatic markers of bone metabolism and effect on mineral composition. A total of 300 unsexed day-old arbor acre broiler birds were used for this research. The birds were randomly distributed using a completely randomized design into five dietary treatment of six replicates with 10 birds per replicate. Treatment one (T1) served as the control group with 0% taurine supplement. Treatments 2, 3, 4, and 5 contained dietary taurine supplements at 0.002, 0.004, 0.006, and 0.008%. Antibody titre against NCDV and IBDV were determined according to standard procedures. The study lasted 42 days. Birds on 0.002% taurine had the highest antibody titre (128.38) against NCDV, while birds on the 0.006% taurine-supplemented-diet also portrayed a distinct (p<0.05) titre value (1029) against IBDV. Serum alkaline phosphatase and bone specific alkaline phosphatase (132.74 and 150.66) at the 42nd day were highest (p<0.05) for birds on 0.004 and 0.002% dietary taurine supplement respectively. The activity of serum tartrate resistant acid phosphatase (44.94) was notably highest (p<0.05) for birds on 0.008% taurine. Bone mineral contents showed that birds fed with 0.002% taurinesupplemented- diet had the highest percentage (p<0.05) of phosphorous (9.50), calcium (32.18) and phosphate (21.77) composition. Conclusively, inclusion of taurine as dietary supplement has proven useful not only in enhancing the birds’ immunity against NCDV an IBDV, but also in boosting bone mineral composition of meat type poultry birds.

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The effect of egg powder inclusion in the pre-starter diet on the immune response of male broiler chickens

Archives Animal Breeding ◽

10.7482/0003-9438-56-051 ◽

2013 ◽

Vol 56 (1) ◽

pp. 527-535 ◽

Cited By ~ 1

Author(s):

L. Esmailzadeh ◽

M. Shivazad ◽

A. A. Sadeghi ◽

M. Karimi-Torshizi

Keyword(s):

Immune Response ◽

Disease Virus ◽

Antibody Titre ◽

Broiler Chickens ◽

Relative Weight ◽

Control Group ◽

Infectious Bronchitis ◽

Significant Difference ◽

Bursal Disease ◽

Egg Powder

Abstract. A completely randomised design was performed to evaluate the effect of inclusion of egg powder in the pre-started diet (1–7 day age) on the immune response of male broiler chickens. Experimental pre-starter diets formulated to have 0, 20, 40 or 60 g/kg egg powder. The relative weight of lymphoid organs (spleen and bursa) was reduced, in days 7 and 42 of age, by graded inclusion of egg powder in the pre-starter period (P≤0.05). The highest antibody titre against Newcastle disease virus (NDV) and Infectious bronchitis virus (IBV) in days 7 and 42 of age was for chickens fed the diet containing 60 g/kg egg powder in the pre-starter diet (P≤0.05). At day 7 of age, birds fed the diet containing egg powder had a higher antibody titre against Infectious bursal disease virus (IB) compared to the control group (P≤0.05), but at day 42 of age, there was no significant difference between the treatments (P>0.05). There was no difference (P>0.05) among the treatments for the titre against sheep red blood cells and immune response against dinitrochlorobenzene. It was concluded that the inclusion of egg powder to the pre-starter diet could significantly enhance the immune response of the male broiler chickens.

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ALKALINE PHOSPHATASE AS A PROGNOSTIC MARKER IN CANCER BREAST PATIENTS IN A TERTIARY CARE HOSPITAL

10.36106/gjra/9400510 ◽

2021 ◽

pp. 14-16

Author(s):

Asha Premlata Omega Oraon ◽

Bela Rose Ekka

Keyword(s):

Alkaline Phosphatase ◽

Cancer Patients ◽

Prognostic Marker ◽

Prognostic Markers ◽

Tertiary Care Hospital ◽

Serum Alkaline Phosphatase ◽

Tertiary Care ◽

Control Group ◽

Care Hospital ◽

Cancer Breast

OBJECTIVE: To estimate the value of Alkaline Phosphatase in cancer breast patients in a tertiary care hospital. MATERIAL AND METHODS: The study was conducted to estimate the value of Serum Alkaline Phosphatase in 50 cancer breast patients and 50 normal patients of same age as a control group. RESULTS: The level of serum Alkaline Phosphatase was signicantly increased (p<0.05)in cancer patients compared to the control group. CONCLUSION: There is an increase in serum Alkaline Phosphatase in cancer patients compared to the control group and can be a prognostic markers for the progress of the disease.

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PUBERTAL GROWTH AS REFLECTED BY SIMULTANEOUS CHANGES IN BONE MINERAL CONTENT AND SERUM ALKALINE PHOSPHATASE

Acta Paediatrica ◽

10.1111/j.1651-2227.1980.tb07028.x ◽

1980 ◽

Vol 69 (1) ◽

pp. 49-52 ◽

Cited By ~ 35

Author(s):

S. KRABBE ◽

C. CHRISTIANSEN ◽

P. RØDBRO ◽

I. TRANSBØL

Keyword(s):

Alkaline Phosphatase ◽

Bone Mineral Content ◽

Bone Mineral ◽

Mineral Content ◽

Serum Alkaline Phosphatase ◽

Pubertal Growth

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Abnormal Bone Mineral Metabolism after Long-Term Anticonvulsant Treatment

Annals of Pharmacotherapy ◽

10.1177/106002809402800109 ◽

1994 ◽

Vol 28 (1) ◽

pp. 47-48 ◽

Cited By ~ 7

Author(s):

Christopher P. Alderman ◽

Catherine L. Hill

Keyword(s):

Alkaline Phosphatase ◽

Serum Concentration ◽

Bone Mineral ◽

Serum Alkaline Phosphatase ◽

Mineral Metabolism ◽

Bone Mineral Metabolism ◽

Hepatic Microsomal Enzyme ◽

Grand Mal ◽

Anticonvulsant Treatment

OBJECTIVE: To present a case of anticonvulsant-induced disturbances of bone mineral metabolism associated with long-term phenytoin treatment. CASE SUMMARY: An 87-year-old woman was hospitalized with generalized acroparesthesia. Her medical history was significant for grand mal epilepsy, which had been treated with phenytoin for more than ten years. On admission she was found to be hypocalcemic, and her alkaline phosphatase concentration was markedly elevated. DISCUSSION: Further investigations revealed that the patient's serum concentration of 25-hydroxycalciferol was well below the expected range. Phenytoin treatment was withdrawn, and calcitriol supplementation commenced. Ten weeks later she was normocalcemic, and the calcitriol dosage was reduced. Radiologic investigations at this time revealed an ununited hip fracture, as well as widespread evidence of bone demineralization. CONCLUSIONS: Minor elevations of liver enzymes observed in association with anticonvulsant treatment may reflect hepatic microsomal enzyme induction. Marked elevation of serum alkaline phosphatase, particularly when seen in concert with hypocalcemia, may be markers of anticonvulsant-induced bone disease. Under these circumstances, further radiologic investigations and measurement of the vitamin D serum concentration should be undertaken.

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Bone mineral density and biochemical markers of bone metabolism in predialysis patients with chronic kidney disease

Journal of Investigative Medicine ◽

10.1136/jim-2015-000043 ◽

2016 ◽

Vol 64 (4) ◽

pp. 861-866 ◽

Cited By ~ 7

Author(s):

Nuri Fidan ◽

Ayca Inci ◽

Melahat Coban ◽

Cevval Ulman ◽

Seyhun Kursat

Keyword(s):

Bone Mineral Density ◽

Chronic Kidney Disease ◽

Alkaline Phosphatase ◽

Kidney Disease ◽

Bone Loss ◽

Bone Mineral ◽

Serum Alkaline Phosphatase ◽

Mineral Density ◽

T Score ◽

Z Scores

The aim of the study was to evaluate the usefulness of serum bone turnover markers (BTM) and bone mineral density (BMD) determined by dual-energy X-ray absorptiometry (DEXA) in predialysis patients with chronic kidney disease (CKD). We enrolled 83 patients with CKD, 41 (49.4%) males, 42 (50.6%) females, with mean estimated glomerular filtration rate (eGFR) 23.90±12 (range=6.0–56.0). BMD of the lumbar spine (LS) (anteroposterior, L2 through L4), femoral neck (FN) and femoral trochanter (FT) were measured by DEXA. Biochemical BTM, including calcium (Ca), phosphorus (P), intact parathyroid hormone (PTH), serum specific alkaline phosphatase (serum AP), bone-specific AP (BSAP), plasma bicarbonate and 25-hydroxy-vitamin D (25hD) were used for the prediction of BMD loss. T score results of LS and FN were worse than FT. BMD levels were lower in females than in males (all p<0.05). According to different BMD T score levels, patients with age ≥65 years and patients in menopause were significantly more osteopenic (p=0.026) and there was no relation between different BMD T scores and presence of diabetes (p=0.654). A positive correlation was identified between the BMD of FN T-Z scores (r=0.270, p=0.029, r=0.306, p=0.012), FT T-Z scores (r=0.220, p=0.076, r:0.250, p=0.043) and serum HCO3, while the correlation with serum alkaline phosphatase (AP) and BSAP was considered to be negative. No statistically significant association was found between BMD of all the measured skeletal sites and eGFR. Loss of BMD was identified mostly in females over ≥65 years of age and after menopause. Higher serum levels of BSAP and AP can be determined in the advanced stages of renal failure and they reflect fracture risk of the femur, but not spine. Measurements of BMD by DEXA are useful to demonstrate bone loss, but not technical enough to distinguish the quantity of bone loss between different stages of CKD.

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Psoralen and Isopsoralen Ameliorate Sex Hormone Deficiency-Induced Osteoporosis in Female and Male Mice

BioMed Research International ◽

10.1155/2016/6869452 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Xiaomei Yuan ◽

Yanan Bi ◽

Zeman Yan ◽

Weiling Pu ◽

Yuhong Li ◽

...

Keyword(s):

Serum Alkaline Phosphatase ◽

Bone Destruction ◽

Hormone Deficiency ◽

Estradiol Valerate ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase ◽

Sham Operation ◽

Type I ◽

Male Mice ◽

Treatment Of Osteoporosis

Osteoporosis is a systemic skeletal disease, which is characterized by a systemic destruction of bone mass and microarchitecture. With life standard improved, the treatment of osteoporosis attracted more attention. The aim of this study is to verify the osteoprotective effect of psoralen and isopsoralen in females and males. Female and male mice were divided into 7 groups in this study: control group (sham-operation), model group (by ovariectomy or orchidectomy), positive control group (females given estradiol valerate; males given alendronate sodium), psoralen groups (10 mg/kg and 20 mg/kg), and isopsoralen groups (10 mg/kg and 20 mg/kg). After administration of psoralen and isopsoralen for 8 weeks, osteoporosis was ameliorated with increasing bone strength and improving trabecular bone microstructure as indicated by CT scan and pathology. Serum alkaline phosphatase (ALP), tartrate resistant acid phosphatase (TRACP), osteocalcin (OC), and C-terminal cross-linking telopeptides of type I collagen (CTX-1) were examined. Decreased TRACP and increased ALP/TRACP suggested restoring from bone destruction. These results suggest that psoralen and isopsoralen may be used as good natural compounds for the treatment of osteoporosis in males, as well as females.

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Effect of Intermittent Fasting on Glucose Homeostasis and Bone Remodeling in Glucocorticoid-Induced Osteoporosis Rat Model

Journal of Bone Metabolism ◽

10.11005/jbm.2021.28.4.307 ◽

2021 ◽

Vol 28 (4) ◽

pp. 307-316

Author(s):

Majed G. Alrowaili ◽

Abdelaziz M. Hussein ◽

Elsayed A. Eid ◽

Mohamed S. Serria ◽

Hussein Abdellatif ◽

...

Keyword(s):

Bone Remodeling ◽

Bone Mineral ◽

Serum Levels ◽

Male Rats ◽

Control Group ◽

Intermittent Fasting ◽

Tartrate Resistant Acid Phosphatase ◽

Type I ◽

Mineral Density ◽

Trap 5B

Background: The present study examined the effect of intermittent fasting (IF) on bone mineral content (BMC) and bone mineral density (BMD) and the markers of bone remodeling in a glucocorticoid-induced osteoporosis (GIO) rat model.Methods: Forty male rats were allocated to 4 groups (N=10 per group): control group of normal rats; control+IF group (normal rats subjected to IF for 16-18 hr daily for 90 days); dexamethasone (DEX) group: (DEX [0.5 mg i.p.] for 90 days); and DEX+IF group (DEX and IF for 90 days). By the end of the experiment, BMD and BMC in the right tibia were measured. Serum levels of the following were measured: glucose; insulin; triglycerides (TGs); total cholesterol; parathyroid hormone (PTH); osteoprotegerin (OPG); receptor activator of nuclear factor-κB (RANK); bone-resorbing cytokines, including bone deoxypyridinoline (DPD), N-terminal telopeptide of collagen type I (NTX-1), and tartrate-resistant acid phosphatase 5b (TRAP-5b); and bone-forming cytokines, including alkaline phosphatase (ALP) and osteocalcin (OC).Results: DEX administration for 90 days resulted in significantly increased serum levels of glucose, insulin, TGs, cholesterol, PTH, OPG, DPD, NTX-1, and TRAP-5b and significantly decreased BMD, BMC, and serum levels of RANK, OC, and ALP (all P<0.05). IF for 90 days significantly improved all these parameters (all P<0.05).Conclusions: IF corrected GIO in rats by inhibiting osteoclastogenesis and PTH secretion and stimulating osteoblast activity.

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Serum uric acid, lipid profile and alkaline phosphatase levels in ischemic cerebrovascular accident patients

International Journal of Advances in Medicine ◽

10.18203/2349-3933.ijam20212862 ◽

2021 ◽

Vol 8 (8) ◽

pp. 1171

Author(s):

Gaurav Gupta ◽

Saurabh Kishor ◽

Aditya Kumar

Keyword(s):

Alkaline Phosphatase ◽

Uric Acid ◽

Ischemic Stroke ◽

Serum Uric Acid ◽

Cerebrovascular Accident ◽

Serum Alkaline Phosphatase ◽

Multiple Logistic Regression Analysis ◽

Elevated Serum ◽

Control Group ◽

Significant Difference

Background: Stroke or cerebrovascular accident (CVA) is noted as the second cause of mortality, especially in the elderly population. Recent studies indicated that higher concentrations of uric acid are involved in various vascular diseases. The findings of previous investigations suggest that, elevated serum alkaline phosphatase (ALP) levels may have a pathophysiological character in the occurrence of atherosclerotic vascular disease (AVD) of the heart and brain. This study evaluated the association between serum uric acid (SUA) levels, serum lipid levels, serum alkaline phosphatase (ALP) levels, and changes in ischemic cerebrovascular accident patients.Methods: All patients with Ischemic cerebrovascular accident age >50 years were included based on their clinical, laboratory, and radiological findings (including computed tomography (CT)/magnetic resonance imaging (MRI)) those admitted in our hospital. As control group 200 healthy individuals matched for sex and age were recruited from the same demographic area.Result: Multiple logistic regression analysis findings proposed four components as significant predictors in ischemic cerebrovascular accident (serum uric acid, serum ALP, LDL and HDL. In this study, it was found, that patients with ischemic cerebrovascular accident had significant difference (p<0.001) in serum uric acid and serum ALP than normal patients (non-ischemic cerebrovascular accident patients).Conclusions: Patients with ischemic cerebrovascular accident had significant difference (p<0.001) in SUA and serum ALP than normal patients (non-ischemic cerebrovascular accident patients). High SUA levels were observed to be associated significantly with ischemic stroke. On the basis of our study design, we cannot clarify that the elevated levels are the risk of ischemic stroke and it requires further studies.

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309 Effects of dietary supplying icariin on the bone metabolism and the laying performance in caged laying hens

Journal of Animal Science ◽

10.1093/jas/skz258.020 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 11-11

Author(s):

Jie Huang ◽

Lei Zhang ◽

Zhongxin Zhou

Keyword(s):

Bone Mineral Density ◽

Bone Metabolism ◽

Bone Mineral ◽

Laying Hens ◽

Egg Quality ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase ◽

Mineral Density ◽

Laying Performance ◽

Serum Biochemical

Abstract Icariin, a flavonol glycoside, is one of major active ingredients of the traditional Chinese medicine Herba epimedii. Icariin has been reported to successfully treat the osteoporosis of the rat. However, effects of icariin on the osteoporosis in caged laying hens are still unkown. This study present the effects of dietary icariin supplementation on the laying performance, the egg quality and the bone metabolism in caged laying hens. A total of 216 Lohmann pink-shell laying hens of 54-week-old from a commercial farm in the Hubei province of China were randomly assigned to 3 treatment groups with 6 replications per group and 12 birds per replication. The control group was fed a corn-soybean meal basal diet, and the experimental groups were fed basal diets supplemented with 500 and 2000 mg/kg icariin for 90 d. Layer performance responses, egg quality parameters, the bone mineral density and serum biochemical indicators were measured at the end of the experiment. Results showed that feed/egg ratio decreased as the supplied icariin level increased. The laying rate and the average egg weight were increased compared to the control group. However, no significant effect was observed on the egg quality. The bone mineral density of the tibia was measured by the dual-energy X-ray absorptiometry, indicating that icariin can increase the bone mineral density. Serum biochemical analysis showed that icariin decreased the level of alkaline phosphatase, tartrate-resistant acid phosphatase, osteocalcin and calcitonin. Our observations provided evidences that dietary supplementation of icariin increased the bone mineral density and improved the laying performance, and icariin can be used for the prevention of the osteoporosis in caged laying hens.

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