The bone strengthening effects of propolis in ovariectomized female white rats as models for postmenopause

Abstract Osteoporosis is a bone disease characterized by decreased quality and strength of bones so that it becomes porous and fracture. Propolis is known to have many pharmacological activity, including an anti-osteoporosis effect. This study aims to determine the effect of propolis administration and the effects of propolis dosage variation in preventing osteoporosis based on the strength value of femur bone impact in female white rats in the form of an ovariectomy postmenopausal model. The rats were divided into 5 groups: positive control group (subjected to ovariectomy), negative control group (not subjected to ovariectomy, and treatment groups that were subjected to ovariectomy and given propolis at a dose of 180 mg/kg BW, dose 360 mg/kg BW and dose 720 mg/kg BW. Propolis was administered orally for 30 days. Bone impact strength testing was undertaken after 30 days using an impact testing machine. Research data were analyzed via one-way ANOVA and continued with the Duncan’s Multiple Range Test. From the test results, we noted that propolis administration had an effect on the value of bone strength, with the dose of 720 mg/kg BW and 360 mg/kg BW having a significant effect, compared with others. With an increase in dose, propolis can provide an increase in the value of bone strength in rat bones.

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Utilization of Honey Apis dorsata as Antiosteoporosis on Requirements of Bone Calcium Ash Density on Ovariohysterectomized White Rat (Ratus norvegicus)

KnE Life Sciences ◽

10.18502/kls.v3i6.1191 ◽

2017 ◽

Vol 3 (6) ◽

pp. 627 ◽

Cited By ~ 1

Author(s):

Muhammad Huda Ramadhan Ibrahim ◽

Abdullah Hasib Hasib ◽

Siti Nur Rohmah ◽

Salsabilla Abani ◽

Samsi Yordan ◽

...

Keyword(s):

Lumbar Vertebrae ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Ash Content ◽

Control Group ◽

Apis Dorsata ◽

Treatment Groups ◽

White Rats ◽

Bone Calcium

The purpose of this study was to determine the effect of honey bee Apis dorsata as anti-osteoporosis in calcium ash density (CAD) of bone in osteoporotic-induced rats. The target of this study was to know bone calcium levels after being given honey bees Apis dorsata. In this study, 35 female white rats (Ratus norvegicus) mature was used with weight 200gr. Divided into 5 groups, 2 control groups and 3 treatment groups. The negative control group (SH) was not induced by osteoporosis and was given the only aquadest of 1.5 ml/day. Whereas the positive control group was induced by osteoporosis (OH) and was given only aquadest 1.5 ml/day. T1, T2 and T3 treatment groups were given bee honey with various doses including 1g / kg ad 1.5 ml aquadest, 2g / kg BB ad 1.5 ml aquadest and 3g / kg BB ad 1.5 ml aquadest. Then after 12 weeks, white rats were sacrificed for lumbar vertebrae. Furthermore, the sixth lumbar spine os vertebrae will be examined for calcium bone ash content. The data were obtained was analyzed using statistical analysis of variance (ANOVA) for the results of the calcium ash content data Keywords: Honey; Osteoporosis; CAD

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AKTIVITAS ANTI-INFLAMASI EKSTRAK ETANOL TEPUNG PELEPAH AREN (Arenga pinnanta)

CHEMISTRY PROGRESS ◽

10.35799/cp.13.2.2020.31894 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Devy Angreani M ◽

Meiske Sangi ◽

Feti Fatimah

Keyword(s):

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Control Group ◽

Treatment Groups ◽

White Rats ◽

Wistar Strain ◽

Anti Inflammatory

Tepung pelepah aren secara tradisional digunakan sebagai obat untuk menghilangkan rasa gatal dan luka bakar pada kulit. Penelitian ini bertujuan untuk menguji aktivitas anti-inflamasi ekstrak etanol tepung pelepah aren (Arenga pinnanta) menggunakan metode induksi karagenan. Ekstrak etanol tepung pelepah aren yang digunakan didapatkan dari hasil maserasi tepung pelepah aren menggunakan etanol hasil redestilasi cap tikus. Metode pengujian aktivitas anti-inflamasi menggunakan 15 ekor tikus putih jantan galur wistar dengan berat 150-200 gram yang dibagi dalam 5 kelompok perlakuan, Kelompok Kontrol negative, Kontrol Positif dan kelompok dosis ekstrak etanol 20%, 25% dan 30%. Hasil penelitian ini menunjukkan kelompok ekstrak etanol tepung pelepah aren dosis 30% memiliki kemampuan inhibisi udem yang sama dengan kontrol positif (Na Diklofenak) dan memiliki kemampuan inhibisi yang lebih besar dibandingkan dosis 20% dan 25%.ABSTRACTPalm stem flour is traditionally used as a remedy for the relief of itching and burns on the skin. This research aims to test the anti-inflammatory activity of palm-flour (Arenga pinnanta) ethanol extract using Caragenan induction method. Palm-dried stem flour extract used from the maceration of palm flour, using ethanol redestilation of rat seals. The method of testing anti-inflammatory activity using 15 male white rats with Wistar strain with a weight of 150-200 grams divided into 5 treatment groups, negative control group, positive control and group dose of ethanol extract 20%, 25% and 30%. The results of this study showed that the group of dried palm flour extract dose 30% have the same inhibition capability with positive control (Na Diklofenak) and have greater inhibition capability compared to the dose 20% and 25%.

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Histopathology of Heart Rats (Rattus norvegicus) Due to the Use of Cooking Oil

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v8i1.3346 ◽

2008 ◽

Vol 8 (1) ◽

Author(s):

Siti Aisyah ◽

Ummu Balqis ◽

Eko Karunia Friyan

Keyword(s):

Rattus Norvegicus ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Test Results ◽

Treatment Groups ◽

Cooking Oil ◽

White Rats ◽

Ad Libitum ◽

Group B

This research aims to study the histopathological picture of rats (Rattus norvegicus) heart as a result of the provision of waste cooking oil3x, 6x, and 9x frying. This study used 20 male white rats (Rattus norvegicus) weighing of ± 300 g. Rats were grouped into 4 treatment groups,adapted for one week, feed and drink ad libitum. The second week to eight week rats were given orally cooking oil every day with a dose of 1 ml. A group of rats was given cooking oil (negative control). Group B, C, and D were given cooking oil 3x, 6x, and 9x frying. Mi ce were euthanizedafter 60 days, then necropsied to collect the heart. Heart was then put into Neutral Buffered Formalin (NBF) for fixation, then follow withhistopathology preparation. Parameters measured were hyperemia, hemorrhage, degeneration, necrosis of the heart. Results were analyzeddescriptively with the scoring system. Test results are grouped into three categories, namely, mild (+), moderate (++), and weight (+++). Thehystopathological results of the heart found were an increasing number of hyperemia, hemorrhage, degeneration, necrosis as a result of thefrequency of frying.____________________________________________________________________________________________________________________Key words: heart, cooking oil, rats

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Uji Aktivitas Repelen Ekstrak Etanol Bunga Marigold (Tagetes erecta) Terhadap Nyamuk Aedes aegypti

Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan ◽

10.33772/pharmauho.v6i2.13339 ◽

2020 ◽

Vol 6 (2) ◽

pp. 54

Author(s):

Mas Arya Shinta

Keyword(s):

Aedes Aegypti ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Tagetes Erecta ◽

Test Results ◽

Dengue Virus Infection ◽

Treatment Groups

AbstractAedes aegypti mosquitoes are vectors of dengue fever caused by dengue virus infection. Marigold flower (Tagetes erecta) is a multipurpose plant, marigold flower has a distinctive smell that is widely used as an insect repellent plant. Marigold flower contains a compound of secondary metabolites that potentially have an effect as a natural insecticide. This study was conducted with a complete random draft by taking five treatments, namely: one negative control group (K-), three treatment groups (p1, P2, P3), and one positive control group (K+). The test results portray the marigold flower ethanol extract has a protection power of 0% on K-; 41.67% on P1; 71.67% on P2; 93.3% on P3; and 100% on K+. Screening phytochemicals against marigold flower ethanol extract identifies positive compounds of alkaloids, flavonoids, saponins, tannins, and triterpenoids. The protection power result shows that the marigold power ethanol extract 10% concentration lotion has a potential to have activity as repellent against Aedes aegypti mosquitoes with protection power above 90%.AbstrakNyamuk Aedes aegypti merupakan vektor dari penyakit Demam Berdarah Dengue (DBD) yang disebabkan oleh infeksi virus dengue. Bunga marigold (Tagetes erecta) merupakan tanaman yang multiguna, bunga marigold memiliki bau khas yang menyengat sehingga banyak digunakan sebagai tanaman pengusir serangga. Bunga marigold mengandung senyawa metabolit sekunder yang berpotensi memiliki efek sebagai insektisida alami. Penelitian dilakukan dengan Rancangan Acak Lengkap (RAL) dengan mengambil lima perlakuan, yaitu satu kelompok kontrol negatif (K-), tiga kelompok perlakuan (P1, P2, P3), dan satu kelompok kontrol positif (K+). Hasil pengujian menunjukkan bahwa ekstrak etanol bunga marigold memiliki daya proteksi pada K- sebesar 0%, P1 sebesar 41,67%, P2 sebesar 71,67%, P3 sebesar 93,3%, dan K+ sebesar 100%. Skrining fitokimia terhadap ekstrak etanol bunga marigold mengidentifikasi positif senyawa alkaloid, flavonoid, saponin, tanin, dan triterpenoid. Hasil pengujian daya proteksi menunjukkan bahwa lotion ekstrak etanol bunga marigold konsentrasi 10% berpotensi memiliki aktivitas sebagai repellent terhadap nyamuk Aedes aegypti dengan daya proteksi diatas 90%.

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Resistance to Fracture of Dental Roots Obturated with Different Materials

BioMed Research International ◽

10.1155/2015/591031 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Berkan Celikten ◽

Ceren Feriha Uzuntas ◽

Kamran Gulsahi

Keyword(s):

Fracture Resistance ◽

Testing Machine ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Root Canal Filling ◽

Filling Materials ◽

Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

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Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

Journal of Basic Medical Veterinary ◽

10.20473/jbmv.v10i2.31812 ◽

2021 ◽

Vol 10 (2) ◽

pp. 36

Author(s):

Alvyan Lantang Anugrah ◽

Hana Eliyani ◽

Budi Utomo ◽

Suherni Susilowati ◽

Maslichah Mafruchati ◽

...

Keyword(s):

Beta Vulgaris ◽

Sertoli Cells ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

Root Extract ◽

Beet Root ◽

White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

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HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

Buletin Veteriner Udayana ◽

10.24843/bulvet.2019.v11.i01.p03 ◽

2019 ◽

pp. 14

Author(s):

I Made Merdana ◽

I Made Kardena ◽

Ketut Budiasa ◽

I Made Dodi Gunawan

Keyword(s):

Rat Liver ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Toxic Dose ◽

White Rats ◽

Significant Difference ◽

Ant Nest ◽

Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

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Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

Journal of Islamic Medicine ◽

10.18860/jim.v1i2.4456 ◽

2017 ◽

Vol 1 (2) ◽

pp. 76-87

Author(s):

Muhammad Nur

Keyword(s):

Treatment Group ◽

Total Sample ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Control Group Design ◽

White Rats ◽

Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

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Percepatan Penutupan Luka Sayat pada Tikus Putih Akibat Pemberian Perasan Rimpang Temulawak (Curcuma xanthorrhiza Roxb.)

Jurnal Pharmascience ◽

10.20527/jps.v8i1.9333 ◽

2021 ◽

Vol 8 (1) ◽

pp. 1

Author(s):

Elisabeth Natalia Barung ◽

Rifny Wungow ◽

Donald Emilio Kalonio

Keyword(s):

Wound Healing ◽

Linear Regression Analysis ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

White Rats ◽

Curcuma Xanthorrhiza ◽

Incision Wound

Rimpang Temulawak atau Curcuma xanthorriza Roxb. adalah tanaman yang dikenal luas oleh masyarakat sebagai obat tradisional. Rimpang temulawak mengandung kurkumin dan xanthorrizzol, yang diketahui mampu mempercepat penutupan luka di kulit dan juga memiliki efek antibakteri dan antiinflamasi. Penelitian ini bertujuan untuk untuk mengetahui efektifitas perasan rimpang temulawak terhadap percepatan penutupan luka sayat pada tikus putih. Penelitian ini adalah penelitian eksperimen, dengan subyek 10 ekor tikus putih yang dibagi dalam 2 kelompok perlakuan yaitu kelompok yang diberi perasan temulawak dan kelompok kontrol negatif yang tidak diberi perlakuan. Data dikumpulkan dengan mengukur panjang luka tikus menggunakan alat ukur penggaris dan dihitung persentasi penutupan luka. Data dianalisis menggunakan analisis regresi linear dan nilai slope (b) dinyatakan sebagai kecepatan penutupan luka. Hasil penelitian ini, menunjukan bahwa rimpang temulawak mampu mempercepat penutupan luka sayat sebesar 15,262%/hari dibandingkan kelompok yang tidak diberi perlakuan sebesar 13,54%/hari. Kata Kunci: Perasan Rimpang Temulawak, Luka Sayat, Percepatan Penutupan Luka, Sediaan Sederhana, Obat Tradisional Indonesia Curcuma xanthorrhiza Roxb. is a plant that is widely known by the community as traditional medicine. The rhizome of C. xanthorrhiza contains curcumin and xanthorrhizol, which are known to be able to accelerate wound healing on the skin and also has antibacterial and anti-inflammatory effects. This study was aimed to determine the effectiveness of C. xanthorriza rhizome on the acceleration of incision wound healing on white rats. This study was an experimental study, with 10 white rat subjects divided into two treatment groups, namely the treatment group which was given by C. xanthorriza rhizome and the negative control group that was not treated. Data were collected by measuring rat wound length using a ruler and calculating the percentage of wound healing. By using linear regression analysis and the value of the slope (b) is expressed as the acceleration of wound healing. The results of the study showed that C. xanthorrhiza rhizome was able to accelerate incision wound healing by 15.262% / day compared to the untreated group of 13.54% / day.

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Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2

Biomolecular and Health Science Journal ◽

10.20473/bhsj.v4i2.26619 ◽

2021 ◽

Vol 4 (2) ◽

pp. 73

Author(s):

Putri Ayu Ika Setiyowati ◽

Rofiatun Solekha ◽

Sri Bintang Sahara Mahaputra Kusuma Negara ◽

Reny Rosalina

Keyword(s):

Body Weight ◽

Optimal Dose ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Antioxidant Compounds ◽

Optimal Dosage ◽

Skin Injury ◽

Treatment Groups

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used. Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of 0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw. In day 21 all group were injected with 0,2 ml of pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

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