scholarly journals Antibacterial and cytotoxic activity of metronidazole and levofloxacin composites with silver nanoparticles

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olexandra Ilkov ◽  
Nazar Manko ◽  
Svitlana Bilous ◽  
Gennadi Didikin ◽  
Olga Klyuchivska ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Electron Beam ◽  
Cytotoxic Activity ◽  
Antimicrobial Agents ◽  
Antibacterial Effect ◽  
Human Cells ◽  
Cytotoxic Action ◽  
Toxic Dose ◽  
Hek 293

Abstract The aim of the work is to to ascertain their antibacterial activity, as well as the toxic effects toward human cells of composites of silver nanoparticles immobilized by electron-beam technology onto crystals of antimicrobial agents metronidazole and levofloxacin The assessment of antibacterial activity and cytotoxic action of silver naonparticled metronidazole and levofloxacin composites was carried out using the MTT-test. Objects of study of antibacterial activity were three strains of microorganisms: Staphylococcus aureus ATCC25923, Escherichia coli dH5α, Pseudomonas aeruginosa ATCC9027. For the investigation of cytotoxic action, cells of HEK 293 line obtained from human kidney embryos were used. Nanocomposites of metronidazole and levofloxacin were tested at concentrations known as the minimum toxic dose of antibiotics and at concentrations reduced/increased in 2 times. Immobilization of silver nanoparticles on the surface of metronidazole and levofloxacin by electron-beam technology gives a different effect on their antibacterial and cytotoxic activity. Nanocomposites of metronidazole exhibit a weaker antibacterial effect on E. coli than metronidazole alone, while levofloxacin nanocomposites have higher antibacterial activity compared to levofloxacin alone. Nanocomposites of the levofloxacin, compared to free levofloxacin, are characterized by a higher antibacterial effect towards gram-negative bacteria (E. coli), but practically do not differ in activity toward P. aeruginosa and S. aureus. Immobilization of silver nanoparticles on metronidazole crystals does not affect on its cytotoxicity relative to pseudonormal human cells line HEK 293, while the nanocomposites of levofloxacin with silver are more toxic to these cells than levofloxacin alone.

Antibacterial Activity of Biodegradable Particles based on Chitosan Containing Colloidal Silver

2020 ◽  
Vol 36 (4) ◽  
pp. 87-93
Author(s):  
V.Yu. Reshetova ◽  
A.F. Krivoshchepov ◽  
I.A. Butorova ◽  
N.B. Feldman ◽  
S.V. Lutsenko ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Polymer Matrix ◽  
Adipic Acid ◽  
Antibacterial Effect ◽  
Academic Excellence ◽  
Colloidal Silver ◽  
Scanning Electron Microscopy Data ◽  
Covalent Bonds ◽  
Agar Diffusion Test

Chitosan beads with colloidal silver nanoparticles inclued in the polymer matrix have been obtained by the introduction of chitosan into an acidified nanosilver sol. Dual interconnection of drops of the resulting solution was then carried out by ionotropic gelation at the first stage and covalent crosslinking of the polymer matrix with adipic acid at the second stage. The surface morphology of the obtained beads was studied by scanning electron microscopy. Data of Fourier transform IR spectroscopy confirmed the formation of covalent bonds between chitosan and adipic acid. The antibacterial activity of obtained beads against S. aureus and E. coli was evaluated using agar diffusion test. It was shown that the сhitosan beads modified with nanostructured silver exhibited an antibacterial effect against the tested strains, and they can be used as a basis for creating biodegradable wound healing dressings with a prolonged antibacterial effect. chitosan, silver nanoparticles, antibacterial activity, wound dressings This work was supported by the "Russian Academic Excellence Project 5-100". The study was carried out with the financial support of the Russian Foundation for Basic Research in the framework of the Scientific Project no. 18-29-18039.

scholarly journals 3-Amino-5-(indol-3-yl)methylene-4-oxo-2-thioxothiazolidine Derivatives as Antimicrobial Agents: Synthesis, Computational and Biological Evaluation

2020 ◽  
Vol 13 (9) ◽  
pp. 229
Author(s):  
Volodymyr Horishny ◽  
Victor Kartsev ◽  
Vasyl Matiychuk ◽  
Athina Geronikaki ◽  
Petrou Anthi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Minimal Bactericidal Concentration ◽  
Active Compounds ◽  
Hek 293 ◽  
E Coli ◽  
Thiazolyl Blue Tetrazolium Bromide

Herein we report the design, synthesis, computational, and experimental evaluation of the antimicrobial activity of fourteen new 3-amino-5-(indol-3-yl) methylene-4-oxo-2-thioxothiazolidine derivatives. The structures were designed, and their antimicrobial activity and toxicity were predicted in silico. All synthesized compounds exhibited antibacterial activity against eight Gram-positive and Gram-negative bacteria. Their activity exceeded those of ampicillin and (for the majority of compounds) streptomycin. The most sensitive bacterium was S. aureus (American Type Culture Collection ATCC 6538), while L. monocytogenes (NCTC 7973) was the most resistant. The best antibacterial activity was observed for compound 5d (Z)-N-(5-((1H-indol-3-yl)methylene)-4-oxo-2-thioxothiazolidin-3-yl)-4-hydroxybenzamide (Minimal inhibitory concentration, MIC at 37.9–113.8 μM, and Minimal bactericidal concentration MBC at 57.8–118.3 μM). Three most active compounds 5d, 5g, and 5k being evaluated against three resistant strains, Methicillin resistant Staphilococcus aureus (MRSA), P. aeruginosa, and E. coli, were more potent against MRSA than ampicillin (MIC at 248–372 μM, MBC at 372–1240 μM). At the same time, streptomycin (MIC at 43–172 μM, MBC at 86–344 μM) did not show bactericidal activity at all. The compound 5d was also more active than ampicillin towards resistant P. aeruginosa strain. Antifungal activity of all compounds exceeded those of the reference antifungal agents bifonazole (MIC at 480–640 μM, and MFC at 640–800 μM) and ketoconazole (MIC 285–475 μM and MFC 380–950 μM). The best activity was exhibited by compound 5g. The most sensitive fungal was T. viride (IAM 5061), while A. fumigatus (human isolate) was the most resistant. Low cytotoxicity against HEK-293 human embryonic kidney cell line and reasonable selectivity indices were shown for the most active compounds 5d, 5g, 5k, 7c using thiazolyl blue tetrazolium bromide MTT assay. The docking studies indicated a probable involvement of E. coli Mur B inhibition in the antibacterial action, while CYP51 inhibition is likely responsible for the antifungal activity of the tested compounds.

scholarly journals A Study on Antibacterial Activity of Silver Nanoparticles

2018 ◽  
Vol 29 (2) ◽  
pp. 37-41
Author(s):  
M Iqbal Hossain ◽  
M Anwar Habib ◽  
Nazimuddin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Reference Drug ◽  
Gram Negative ◽  
Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

scholarly journals Synergistic Antibacterial Effect of Casein-AgNPs Combined with Tigecycline against Acinetobacter baumannii

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1529
Author(s):  
Yu-Hsuan Chen ◽  
Wei-Hsun Wang ◽  
Sheng-Hui Lin ◽  
Yuan-Ting Yang-Wang ◽  
Sung-Pin Tseng ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Acinetobacter Baumannii ◽  
Nosocomial Infections ◽  
Antibacterial Effect ◽  
Combination Index ◽  
Antibacterial Properties ◽  
The Individual ◽  
Synergistic Antibacterial Activity

Acinetobacter baumannii (A. baumannii) is a common and challenging pathogen of nosocomial infections, due to its ability to survive on inanimate objects, desiccation tolerance, and resistance to disinfectants. In this study, we investigated an antibacterial strategy to combat A. baumannii via the combination of antibiotics and silver protein. This strategy used a functional platform consisting of silver nanoparticles (AgNPs) resurrected from silver-based calcium thiophosphate (SSCP) through casein and arginine. Then, the silver protein was combined with tigecycline, the first drug in glycylcycline antibiotic, to synergistically inhibit the viability of A. baumannii. The synergistic antibacterial activity was confirmed by the 96-well checkerboard method to determine their minimum inhibitory concentrations (MIC) and calculated for the combination index (CI). The MIC of the combination of silver protein and tigecycline (0.31 mg/mL, 0.16 µg/mL) was significantly lower than that of the individual MIC, and the CI was 0.59, which indicates a synergistic effect. Consequently, we integrated the detailed synergistic antibacterial properties when silver protein was combined with tigecycline. The result could make for a promising approach for the treatment of A. baumannii.

scholarly journals Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

2016 ◽  
Author(s):  
Patcharaporn Tippayawat ◽  
Nutthakritta Phromviyo ◽  
Parichart Boueroy ◽  
Apiwat Chompoosor
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Hydrothermal Method ◽  
Plant Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Diffusion Method

Background: There is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity. Methods: AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results: The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in pharmaceutical, biotechnological and biomedical applications. Discussion: Aloe vera extract was processed using a green and facile method. This was a hydrothermal method to reduce silver nitrate to AgNPs@AV. Varying the hydrothermal temperature provided the fine spherical shaped nanoparticles. The size of the nanomaterial was affected by its thermal preparation. The particle size of AgNPs could be tuned by varying both time and temperature. A process using a pure AG phase could go to completion in 6h at 200 oC, whereas reactions at lower temperatures required longer times. Moreover, the antibacterial effect of this hybrid nanomaterial was sufficient that it could be used to inhibit pathogenic bacteria since silver release was dependent upon its particle size. The high activity of the largest AgNPs might have resulted from a high concentration of aloe vera compounds incorporated into the AgNPs during hydrothermal synthesis.

scholarly journals The interplay between size and valence state on the antibacterial activity of sub-10 nm silver nanoparticles

2019 ◽  
Vol 1 (6) ◽  
pp. 2365-2371 ◽  
Author(s):  
Hanif Haidari ◽  
Nirmal Goswami ◽  
Richard Bright ◽  
Zlatko Kopecki ◽  
Allison J. Cowin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Valence State ◽  
Antibacterial Effect ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria

The interplay between size and valence state in ∼3 nm silver nanoparticles resulted in the highest antibacterial effect against multi-drug resistant bacteria.

scholarly journals Rapid Method for Detecting Bactericidal Effects of Mangrove Derived Silver Nanoparticles Using Resazurin Microdilution Assay

2021 ◽  
Vol 9 (8) ◽  
pp. 2438-2442
Author(s):  
Asmathunisha N
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Vital Role ◽  
Bacterial Strains ◽  
E Coli ◽  
Inhibition Concentration ◽  
Resazurin Assay ◽  
Bactericidal Effects

Abstract: Nanoparticles plays a vital role in the field of antimicrobial agents against pathogenic microorganisms. Screening of nanoparticles for antimicrobial activities is a time consuming and cumbersome process. Recently, a simple technique of using the dye resazurin has been used as an indicator of bacterial growth for testing antimicrobial activity on microtitre plate. However, this technique does not quantify the microbial load. Therefore, the present work was attempted to find a new antibacterial method employing the dye resazurin assay and haemocytometric counting of microbes for testing silver nanoparticles synthesised from Xylocarpus mekongensis .The bacterial strains E. coli, S. aureus and P. aeruginosa (multi-drug resistant strain) were used to evaluate the screening of mangrove extracts. Minimum inhibition concentration (MIC) was also calculated for the silver nanoparticles using ciproflaxcin as reference antibiotic. The antibacterial activity Xylocarpus mekongensis was carried out against all the three bacteria by the same method and the values were compared with reference antibiotic. The present study has suggested a rapid, dependable, easy and inexpensive method, suitable for testing the antibacterial activity of silver nanoparticles which are promising to develop as new antibacterials. Keywords: Mangroves, Silver, Nanoparticles, Resazurin, Antibacterials

scholarly journals Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized with the Peel Extract of Mango

Materials ◽  
2021 ◽  
Vol 14 (19) ◽  
pp. 5878
Author(s):  
Yage Xing ◽  
Xingmei Liao ◽  
Xiaocui Liu ◽  
Wenxiu Li ◽  
Ruihan Huang ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Research Attention ◽  
E Coli ◽  
Significant Research ◽  
Mango Peel ◽  
And Staphylococcus Aureus ◽  
Peel Extract

The green synthesis of silver nanoparticles (AgNPs) from biological waste, as well as their excellent antibacterial properties, is currently attracting significant research attention. This study synthesized AgNPs from different mango peel extract concentrations while investigating their characteristics and antibacterial properties. The results showed that the AgNPs were irregular with rod-like, spherical shapes and were detected in a range of 25 nm to 75 nm. The AgNPs displayed antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), showing a more significant impact when synthesized with 0.20 g/mL of mango peel extract. Therefore, the antibacterial effect of different diluted AgNP concentrations on the growth kinetic curves of E. coli and S. aureus after synthesis with 0.20 g/mL mango peel extract was analyzed. The results indicated that the AgNP antibacterial activity was higher against S. aureus than against E. coli, while the AgNP IC50 in these two strains was approximately 1.557 mg/mL and 2.335 mg/L, respectively. This research provides new insights regarding the use of postharvest mango byproducts and the potential for developing additional AgNP composite antibacterial materials for fruit and vegetable preservation.

scholarly journals Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Lamia Benhalima ◽  
Sandra Amri ◽  
Mourad Bensouilah ◽  
Rachid Ouzrout
Keyword(s):  
Antibacterial Activity ◽  
Copper Sulfate ◽  
Antimicrobial Agents ◽  
Antibacterial Effect ◽  
Hospital Environment ◽  
Clinical Samples ◽  
Drug Resistant ◽  
Nosocomial Pathogens ◽  
Sulfate Salts ◽  
Effect Of Copper

Background and Objective: With the emergence of antibiotic resistance and the hospital acquired infection, the interest for antimicrobial agents has recently increased again in public health. Copper is recommended as a supplementary method of increasing biological safety in the hospital environment. The objective of this study was to determine the antibacterial activity of copper sulfate salts on strains of bacterial pathogens isolated from different clinical pictures in different health establishment in Algeria. Methods: A total of 25 different bacterial isolates (16 Enterobacteriaceae, 5 Staphylococci, and 4 Pseudomonas) were tested for susceptibility to copper sulfate using minimum inhibitory concentration (MIC-Cu) and minimum bactericidal concentrations (MBC-Cu) determinations. All isolates were also tested for susceptibility to six antibiotics. Results: Antibiotic susceptibility studies revealed that 100% of isolates were resistant to one or more antibiotics. Fifty two percent of isolates were very susceptible to copper sulfate, with MICs ranging from 100 to 200 µg/ml. MBC-Cu = 1600 μg/ml showed the best bactericidal effect against the great majority of studied bacteria (52%). A good bactericidal activities of copper sulfate were recorded against Proteus vulgaris and Staphylococcus aureus (MBC/MIC=1). The Gram-negative bacteria isolates which were copper resistant also showed a high resistance to chloramphenicol (r=0.78) and Trimethoprime (r=0.61). Furthermore, the strains that were no-susceptible to three different antimicrobial classes (Escherichia coli, Staphylococcus saprophyticus) were not resistant to copper sulfate. Conclusion: Copper sulfate salts has significant antibacterial activity against multi-drug resistant nosocomial pathogens. doi: https://doi.org/10.12669/pjms.35.5.336 How to cite this:Benhalima L, Amri S, Bensouilah M, Ouzrout R. Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples. Pak J Med Sci. 2019;35(5):---------.  doi: https://doi.org/10.12669/pjms.35.5.336 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Sign in / Sign up

Export Citation Format

Share Document