scholarly journals Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats

2018 ◽  
Vol 52 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Elham Karimi-Sales ◽  
Sajad Jeddi ◽  
Arshad Ghaffari-Nasab ◽  
Mina Salimi ◽  
Mohammad Reza Alipour
Keyword(s):  
Serum Level ◽  
Proinflammatory Cytokines ◽  
Serum Levels ◽  
Male Rats ◽  
Positive Effects ◽  
Qrt Pcr ◽  
Group Control ◽  
First Time ◽  
Hepatic Level

Abstract Objective. Trans-chalcone is a chalcone with hepatoprotective and anti-inflammatory effects. However, the mechanism of these positive effects, especially on miR-451 as an inflammatory regulator, is poorly understood. In this regard, this microRNA (miRNA) acts by inhibition of hepatic interleukin-8 (IL-8) production in the liver which is one of the main proinflammatory cytokines. Th is study for the first time examined the effect of trans-chalcone on miR-451/IL-8 pathway. Methods. In present study, 21 male rats were randomly divided into 3 groups (n=7 per each group): control which received solvent (NS), groups 2 (N2T) and 3 (N6T), which received transchalcone for 2 and 6 weeks, respectively. Hepatic level of miR-451 was measured by qRT-PCR. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as hepatic level of IL-8 protein were measured. Results. Trans-chalcone decreased hepatic level of IL-8 protein and serum level of ALT aft er 2 weeks of treatment without significant change in hepatic miR-451. Moreover, it increased hepatic level of miR-451 and reduced hepatic IL-8 as well as AST and ALT aft er 6 weeks. Conclusion. Based on the results of present study, miR-451/IL-8 pathway is a possible mechanism for hepatoprotective action of trans-chalcone in long-term.

scholarly journals Immunohistochemical Review of Leydig Cell Lesions in Ochratoxin A-Treated Fischer Rats and Controls

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 480 ◽  
Author(s):  
Diana Herman ◽  
Peter Mantle
Keyword(s):  
Testicular Cancer ◽  
Ochratoxin A ◽  
Leydig Cell ◽  
Dietary Exposure ◽  
Male Rats ◽  
Testicular Hypertrophy ◽  
Cellular Pathology ◽  
Rats And Mice ◽  
First Time

Ochratoxin A is best known as a potent renal carcinogen in male rats and mice after necessarily protracted ingestion, although valid extrapolation to any human disease has not been verified. The hypothesis that the toxin is a cause of human testicular cancer was proposed a decade ago and has proliferated since, partly through incomplete study of the scientific literature. Archived tumorous rat testes were available from Fischer F344 rats exposed to continuous dietary exposure for half of or the whole life in London in the 2000s. Renal cancer occurred in some of these cases and testicular tumours were observed frequently, as expected, in both treated and untreated animals. Application of clinical immunohistochemistry has for the first time consistently diagnosed the testicular hypertrophy in toxin-treated rats as Leydig cell tumours. Comparison is made with similar analysis of tumorous testes from control (untreated) rats from U.S. National Toxicology Program studies, both of ochratoxin A (1989) and the more recent one on Ginkgo biloba. All have been found to have identical pathology as being of sex cord-stromal origin. Such are rare in humans, most being of germinal cell origin. The absence of experimental evidence of any specific rat testicular cellular pathology attributable to long-term dietary ochratoxin A exposure discredits any experimental animal evidence of testicular tumorigenicity. Thus, no epidemiological connection between ochratoxin A and the incidence of human testicular cancer can be justified scientifically.

scholarly journals Diene Valepotriates fromValeriana glechomifoliaPrevent Lipopolysaccharide-Induced Sickness and Depressive-Like Behavior in Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Liz G. Müller ◽  
Milene Borsoi ◽  
Eveline D. Stolz ◽  
Vivian Herzfeldt ◽  
Alice F. Viana ◽  
...  
Keyword(s):  
Proinflammatory Cytokines ◽  
Native Species ◽  
Southern Brazil ◽  
Behavioral Alterations ◽  
Bdnf Expression ◽  
E Coli ◽  
Positive Effects ◽  
Vulnerable Patients ◽  
First Time ◽  
Stressful Stimulus

Valeriana glechomifolia, a native species from southern Brazil, presents antidepressant-like activity and diene valepotriates (VAL) contribute to the pharmacological properties of the genus. It is known that depression can develop on an inflammation background in vulnerable patients and antidepressants present anti-inflammatory properties. We investigated the effects of VAL (10 mg/kg, p.o.) on sickness and depressive-like behaviors as well as proinflammatory cytokines (IL-1βand TNF-α) and BDNF expression in the cortex of mice exposed to a 5 min swimming session (as a stressful stimulus) 30 min before theE. coliLPS injection (600 µg/kg, i.p.). The forced swim + LPS induced sickness and depressive-like behaviors, increased the cortical expression of IL-1βand TNF-α, and decreased BDNF expression. VAL was orally administered to mice 1 h before (pretreatment) or 5 h after (posttreatment)E. coliLPS injection. The pretreatment with VAL restored the behavioral alterations and the expression of cortical proinflammatory cytokines in LPS-injected animals but had no effects on BDNF expression, while the posttreatment rescued only behavioral alterations. Our results demonstrate for the first time the positive effects of VAL in an experimental model of depression associated with inflammation, providing new data on the range of action of these molecules.

To the Richest Go the Spoils? Immigration, Development, and the North-South Divide, 1970–2005

2016 ◽  
Vol 2 (3) ◽  
pp. 273-292
Author(s):  
Matthew R. Sanderson
Keyword(s):  
Economic Development ◽  
Global South ◽  
Positive Effects ◽  
The North ◽  
National Economic ◽  
Global North ◽  
First Time ◽  
The Relationship ◽  
North And South

This paper empirically assesses, for the first time, the relationship between immigration and national economic development in both the global North and the global South. A series of panel models demonstrate that immigration exacerbates North-South inequalities through differential effects on average per capita incomes in the global North and global South. Immigration has positive effects on average incomes in both the North and the South, but the effect is larger in the global North. Thus the relationship between immigration and development evinces a Matthew Effect at the world level: by contributing to differential levels of economic development in the North and South, immigration widens international inequalities in the long term, resulting in the accumulation of advantage in the North. The implications of the results are discussed in the context theory and policy on the migration-development nexus.

scholarly journals Increased Serum Levels of S100A4 and S100A15 in Individuals Suffering from Hidradenitis Suppurativa

2021 ◽  
Vol 10 (22) ◽  
pp. 5320
Author(s):  
Aleksandra Batycka-Baran ◽  
Łukasz Matusiak ◽  
Danuta Nowicka-Suszko ◽  
Jacek C. Szepietowski ◽  
Wojciech Baran
Keyword(s):  
Serum Level ◽  
Hidradenitis Suppurativa ◽  
Inflammatory Diseases ◽  
Serum Levels ◽  
Inflammatory Skin Disease ◽  
Elisa Kit ◽  
Severity Of Disease ◽  
Immune Mediated ◽  
Severity Of The Disease ◽  
First Time

Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease. Recently, some S100 proteins have been suggested to play an important role in the pathogenesis of chronic immune-mediated inflammatory diseases and they may constitute valuable biomarkers for these diseases’ diagnosis and monitoring. The objective of the current study was to investigate, for the first time, serum levels of S100A4 and S100A15 in individuals suffering from HS. Furthermore, we assessed the associations between S100A4 and S100A15 serum levels and the severity of disease, CRP serum concentration and some demographic and clinical data. Serum levels of S100A4 and S100A15 were evaluated with the commercially available ELISA kit according to the manufacturer’s instructions. The serum level of S100A4 in individuals with HS was significantly elevated as compared to controls, with the highest level found in the individuals in Hurley stage II. The S100A15 serum level was positively correlated with the CRP concentration and was associated with the severity of the disease. The serum level of S100A15 in the individuals in Hurley stage III was significantly elevated compared to that of the controls and the individuals with HS in Hurley stages I and II. S100A4 and S100A15 may be considered as new serum biomarkers for the monitoring of HS progression, and they may play a role in the pathogenesis of HS by promoting inflammatory process and fibrosis.

scholarly journals Prevalence of Insufficient Serum 25-hydroxyvitamin D (25(OH)D) Greater Among Medicaid Residents Living in Long-Term Care Communities (P01-011-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Ronna Robbins ◽  
Nalini Ranjit ◽  
Sara Sweitzer ◽  
Maragaret Briley
Keyword(s):  
Logistic Regression ◽  
Serum Level ◽  
Medical Records ◽  
Long Term Care ◽  
Serum Levels ◽  
Term Care ◽  
Hydroxyvitamin D ◽  
Payer Source ◽  
25 Hydroxyvitamin D

Abstract Objectives Evaluate the association between insufficient 25-hydroxyvitamin D [25(OH)D] serum levels and healthcare payer source of older adults living in long-term care (LTC) communities. Methods Residents (age >65 yo) of five LTC communities in Central, Texas were recruited to participated in the multi-site, cross-sectional study. A one-year medical history was abstracted from medical records using double-blinded data abstraction and entry protocols. Medical history included but not limited to: diagnosis, medications, history of supplementation, BMI, mini-nutritional assessment, diet order, total mood assessments, hospitalizations and number of infections. Data on payer source and other demographics were also obtained via medical records. Blood draws were collected to measure serum 25(OH)D levels. Logistic regression models were uses to assess the association between insufficient25(OH)Dserum levels (defined as <30 ng/ml) and healthcare payer source. Total vitamin D supplemented per day along with amount provided in meals, body mass index, race, gender, age, years living in community, and diagnosis of liver and renal disease were used as confounders. Results The 174 participants (89% Caucasian, mean age 83 yo) included 63% females. Payer source was distributed as follows: 55% private pay, 8.6% Medicare, 35% Medicaid, and 1% insurance. Fifty-five % had insufficient25(OH)D serum levels (mean serum level = 32.6 ng/ml; mean supplementation rate of 1138 IU per/d). Insufficient serum levels were seen in 48% of participants with private pay (mean serum level = 36 mg/ml) and 58% with Medicaid (mean serum level = 30.5 ng/ml). Adjusted logistic regression showed that payer source was a significant determinant of insufficient25(OH)D serum levels. Medicaid residents had significantly greater odds of having insufficient 25(OH)D serum levels (adjusted odd ratio (OR) 3.26; CL: 1.25, 8.48; P = 0.015) than private pay participants. Conclusions Practitioners working in LTC can use these results to ensure equity in the provision of medical nutritional therapy across Medicaid residents and private pay residents. Funding Sources Funding for study was provided through the private funds of research team.

THE THYROTROPHIN RESPONSE TO THYROTROPHIN RELEASING HORMONE DURING TREATMENT IN PATIENTS WITH GRAVES' DISEASE

1977 ◽  
Vol 85 (2) ◽  
pp. 335-344 ◽  
Author(s):  
E. Haug ◽  
H. M. M. Frey ◽  
T. Sand
Keyword(s):  
Serum Level ◽  
Thyroid Hormones ◽  
Pituitary Gland ◽  
Serum Levels ◽  
Graves Disease ◽  
Trh Test ◽  
Thyrotrophin Releasing Hormone ◽  
Releasing Hormone ◽  
First Time ◽  
Serum Tsh

ABSTRACT Thyrotrophin releasing hormone (TRH) tests were performed at 4 or 8 weeks intervals, after the initiation of anti-thyroid treatment in 15 patients with Graves' disease. All TRH tests were negative as long as the serum levels of thyroxine (T4) and triiodothyronine (T3) were elevated, and normalization of the serum levels of these hormones always occurred before the response to iv TRH was restored. In 13 patients the time from the patients for the first time were registered as biochemically euthyroid varied from 0–9 months (mean 3.1 months), before normal TRH response was restored. Two patients were still TRH non-responsive at the end of the study, even though they had been biochemically euthyroid for as long as 17 and 18.5 months. The TRH test, therefore, is not helpful in the evaluation of the effect of anti-thyroid treatment in patients with Graves' disease. There was an increase in the serum level of thyrotrophin (TSH) from 3.4 ± 0.3 (sem) to 4.3 ± 0.5 (sem) ng/ml (P <0.05), and a decrease in the serum level of total T4 from 19.4 ± 1.1 (sem) to 5.8 ± 0.8 (sem) μg/100 ml in 13 patients from the first examination until the last time they were examined before restored TRH response. This finding shows that the pituitary gland has retained its ability to synthesize and secrete TSH even though no TSH could be released by iv TRH. In 6 TRH non-responsive patients with Graves' disease, serum TSH levels were suppressed from 2.5 ±1.2 (sem) ng/ml before the administration of a single dose of 3 mg T4 orallly, to 0.9 ± 0.2 (sem) ng/ml, 7 days after the T4 administration. Thus, the negative feed-back effect on the pituitary gland of the thyroid hormones is operating in these patients. This finding indicates that the TRH non-responsiveness in euthyroid patients with Graves' disease is not due to pituitary depletion of TSH, since the negative feed-back effect of the thyroid hormones is operating normally.

scholarly journals Metabolic Effects of Methanol Extract of Harmal (Peganum Harmal ) in Rats Fed With Normal Diet

2020 ◽  
Vol 26 (4) ◽  
pp. 414-431
Author(s):  
Esmail Mollashahi ◽  
◽  
Hamid Reza Kazerani ◽  
Keyword(s):  
Serum Level ◽  
Methanol Extract ◽  
Density Lipoprotein ◽  
The Body ◽  
Metabolic Parameters ◽  
Metabolic Effects ◽  
Male Rats ◽  
Serum Lactate Dehydrogenase ◽  
Control Group ◽  
Positive Effects

Aims: The research aimed to investigate the effects of harmal (Peganum harmal ) seed extract on the weight and some metabolic parameters. Methods & Materials: Male rats, in groups of 7, received different doses of the methanol extract of harmal seed (100, 200, and 400 mg/kg/d body weight) or placebo via a gastric tube for 42 days. At the end of the experiment, the weights of the body, liver, spleen, and heart, and also the levels of some serum parameters, including glucose, cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein, insulin, growth hormone, and thyroid hormones, as well as the composition of the carcass were investigated. Findings: The mean weight gain, food intake, and the serum level of triiodothyronine (T3) significantly declined in the rats receiving the extract at 400 mg/kg. In contrast, the serum level of cholesterol in the group increased (P<0.001). The serum levels of glucose were significantly lower in all experimental groups compared to the control. Furthermore, the groups treated with the methanol extract dose-dependently showed higher serum lactate dehydrogenase levels compared to the control (P<0.05). No significant differences were observed regarding other studied parameters compared with the control group. Conclusion: This research suggests positive effects for harmal extract on some metabolic parameters and weight. At least one of the involved mechanisms seems to be declined appetite and hence decreased feed intake.

scholarly journals What is in it for me: firms strategizing for public-private innovation

IMP Journal ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 72-90 ◽  
Author(s):  
Kristin B. Munksgaard ◽  
Majbritt Rostgaard Evald ◽  
Ann Højbjerg Clarke ◽  
Torben Munk Damgaard
Keyword(s):  
Public Sector ◽  
Private Firms ◽  
Content Type ◽  
Public Network ◽  
The Public ◽  
Positive Effects ◽  
Societal Welfare ◽  
Cross Case Analysis ◽  
First Time

Purpose Public-private innovation (PPI) is often claimed to contribute to societal welfare, to bring positive effects to the public sector and to open new markets to private firms. Engaging in public-private relationships for innovation is, however, also recognised as challenging and problematic. The purpose of this paper is to discuss the managerial and strategic challenges faced by private firms when engaging in public-private relationships for innovation. Design/methodology/approach This qualitative study presents ten firms’ engagement in public-private relationships. Half of them are experienced in innovation in public-private relationship, the other half are engaging in public-private relationships for the first time. Cross-case analysis brings insight into how these different types of firms strategize for handling relationships with public partners. Findings Contrary to non-experienced firms, experienced firms deliberately build a long-term strategy for engaging in PPI in expectation of outcome from more than a single project. They not only engage in building an understanding of the public using setting, but also seek to bridge with the public developing and producing setting to a greater extent than less experienced firms. The experienced firms utilise relationships with actors in the wider public network as a device and an asset for overcoming challenges and for reaping benefits from their engagement. Originality/value The study outlines the characteristics of strategizing among the most experienced firms and their special ability to embrace the complexity of the public sector network while working simultaneously on developing innovation for specific public counterparts.

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

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