scholarly journals Analysis of indole derivatives in methanolic extracts from mycelium of Agaricus bisporus cultured in vitro on liquid Oddoux medium

2014 ◽  
Vol 10 ◽  
pp. 66-72 ◽  
Author(s):  
Bożena Muszyńska ◽  
Katarzyna Sułkowska-Ziaja ◽  
Patrycja Hałaszczuk ◽  
Remigiusz Krężałek ◽  
Maciej Łojewski
Keyword(s):  
Agaricus Bisporus ◽  
Total Content ◽  
In Vitro Cultures ◽  
Quantitative Composition ◽  
Indole Derivatives ◽  
Fruiting Bodies ◽  
Qualitative And Quantitative ◽  
Indole Compounds ◽  
Methanolic Extracts

Methanolic extracts obtained from biomass of Agaricus bisporus (J.E. Lange) Imbach cultured in vitro were analyzed for qualitative and quantitative composition of non-hallucinogenic indole compounds in order to compare their amount with fruiting bodies of these species. Extracts demonstrated to contain six indole compounds. Contents of individual compounds ranged from 0.01 to 21.33 mg/100 g d.w. in biomass from in vitro cultures. The quantitatively dominating compounds included: 5-hydroxytryptophan (12.50 mg/100 g d.w.), L-tryptophan (14.00 mg/100 g d.w.) and serotonin (7.00 mg/100 g d.w.). Total content of the remaining indole compounds under analysis in the study was 55.32 mg/100 g d.w.

In vitro cultures and fruiting bodies of culinary-medicinal Agaricus bisporus (white button mushroom) as a source of selected biologically-active elements

2015 ◽  
Vol 52 (11) ◽  
pp. 7337-7344 ◽  
Author(s):  
Bożena Muszyńska ◽  
Agata Krakowska ◽  
Katarzyna Sułkowska-Ziaja ◽  
Włodzimierz Opoka ◽  
Witold Reczyński ◽  
...  
Keyword(s):  
Agaricus Bisporus ◽  
In Vitro Cultures ◽  
Biologically Active ◽  
Fruiting Bodies ◽  
Button Mushroom ◽  
Active Elements ◽  
White Button Mushroom

scholarly journals Composition and Antioxidant Activity of Phenolic Compounds in Fruit of the Genus Rosa L.

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 545
Author(s):  
Mindaugas Liaudanskas ◽  
Irena Noreikienė ◽  
Kristina Zymonė ◽  
Rugilė Juodytė ◽  
Vaidotas Žvikas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Content ◽  
Quantitative Composition ◽  
Rosa Multiflora ◽  
Epicatechin Gallate ◽  
Qualitative And Quantitative ◽  
Fruit Samples

We investigated the qualitative and quantitative composition of phenolic compounds in the fruit of Rosa L. cultivars grown in Lithuania. The highest total content of phenolic compounds (50.13 ± 4.17 mg GAE/g, p < 0.05) was determined in fruit samples of Rosa pimpinellifolia L. cultivar “Single Cherry”. The highest levels of hydroxycinnamic acid derivatives were determined in fruit samples of Rosa rugosa Thunb. cultivars “Dart’s Defender” and “Adam Chodun”. The highest flavonoid content was determined in fruit samples of Rosa multiflora Thunb. cultivar “Nana” and R. multiflora species. The strongest antioxidant activity evaluated by applying DPPH and FRAP assays was determined in fruit extracts of R. pimpinellifolia cultivar “Single Cherry” and R. rugosa cultivar “Adam Chodun”. Qualitative and quantitative analysis of phenolic compounds in Rosa L. fruit was performed by applying UHPLC. The following phenolic compounds were identified in fruit samples: caffeic acid, chlorogenic acid, quercetin, quercitrin, (+)-catechin, (−)-epicatechin, (−)-epicatechin gallate, rutin, phloridzin, and kaempferol-3-O-glycoside. A strong correlation was determined between the total amount of phenolic compounds determined in extracts of the fruit samples of Rosa L. cultivars and the radical scavenging and reducing activity of their extracts in vitro (R = 0.767 and 0.727, respectively, p < 0.05).

scholarly journals Schisandra henryi C. B. Clarke in vitro cultures: a promising tool for the production of lignans and phenolic compounds

2020 ◽  
Vol 143 (1) ◽  
pp. 45-60
Author(s):  
Karolina Jafernik ◽  
Agnieszka Szopa ◽  
Magda Barnaś ◽  
Michał Dziurka ◽  
Halina Ekiert
Keyword(s):  
Culture Conditions ◽  
In Vitro Cultures ◽  
Leaf Extracts ◽  
Qualitative And Quantitative ◽  
Promising Tool ◽  
Methanolic Extracts ◽  
Different Types ◽  
Chinese Plant ◽  
Schisantherin A

Abstract We initiated and optimized in vitro culture conditions of the endemic Chinese plant species—Schisandra henryi C. B. Clarke. Different types of in vitro solid cultures (microshoot and callus), cultivation periods (10, 20, and 30 days), and selected concentrations of BA, IBA, GA3 (0 to 3 mg/l) in the Murashige and Skoog (MS) medium were tested. The presence of dibenzocyclooctadiene lignans (schisandrin, gomisin G, schisantherin A and B, deoxyschisandrin and schisandrin C), dibenzylbutane lignans (hernicine B), aryltetralin lignans (wulignan A1 and A2, epiwulignan A1, enshicine, epienshicine and dimethylwulignan A1), and triterpenoids: kadsuric acid and schisanhenric acid was confirmed by UHPLC–MS/MS analysis. Using HPLC–DAD, the qualitative and quantitative profiles of dibenzocyclooctadiene lignans, phenolic acids and flavonoids in methanolic extracts from biomass were estimated. The maximum total amounts of these groups of metabolites were 873.71, 840.89 and 421.98 mg/100 g DW, respectively. The main compounds were: schisantherin B (max. 622.59 mg/100 g DW), schisantherin A (max. 143.74 mg/100 g DW), neochlorogenic acid (max. 472.82 mg/100 g DW), caftaric acid (max. 370.81 mg/100 g DW), trifolin (max. 138.56 mg/100 g DW) and quercitrin (max. 122.54 mg/100 g DW). The highest total amounts of secondary metabolites estimated in the extracts from in vitro cultures were, respectively, 13.0, 7.0, and 1.4 times higher than in the leaf extracts analyzed for comparison. This is the first report on the biosynthetic potential of cells from Schisandra henryi in vitro cultures.

scholarly journals Physiologically Active Compounds in Four Species of Phellinus

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Katarzyna Sułkowska-Ziaja ◽  
Anna Maślanka ◽  
Agnieszka Szewczyk ◽  
Bożena Muszyńska
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Total Content ◽  
Dry Weight ◽  
Hplc Method ◽  
Indole Compounds ◽  
Methanolic Extracts ◽  
Free Phenolic Acids ◽  
Layer Chromatography ◽  
Physiologically Active Compounds

The content of two groups of compounds with biological activity (non-hallucinogenic indole compounds and free phenolic acids) were analyzed in extracts of fruiting bodies of four species of Phellinus: P. igniarius, P. pini, P. pomaceus and P. robustus. The presence of indole compounds in methanolic extracts was analyzed by high-performance liquid chromatography and thin-layer chromatography coupled with densitometric detection. Three metabolites (serotonin, tryptamine, and L-tryptophan) were identified. The contents of individual indole compounds ranged from 1.70 (tryptamine in P. robustus) to 8.32 mg x 100 g1 dry weight (L-tryptophan in P. robustus). Four free phenolic acids were detected in methanolic extracts by the HPLC method. The total content ranged from 9.9 mg x 100 g1 DW (P. igniarius) to 32.5 mg x 100 g1 DW (P. robustus).

Remediation capacity of Cd and Pb ions by mycelia of Imleria badia, Laetiporus sulphureus, and Agaricus bisporus in vitro cultures

2017 ◽  
Vol 52 (9) ◽  
pp. 617-622 ◽  
Author(s):  
Agata Kryczyk ◽  
Joanna Piotrowska ◽  
Magdalena Sito ◽  
Katarzyna Sulkowska-Ziaja ◽  
Konrad Dobosz ◽  
...  
Keyword(s):  
Agaricus Bisporus ◽  
In Vitro Cultures ◽  
Laetiporus Sulphureus ◽  
Pb Ions

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Variability in the Qualitative and Quantitative Composition and Content of Phenolic Compounds in the Fruit of Introduced American Cranberry (Vaccinium macrocarpon Aiton)

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1379
Author(s):  
Ieva Gudžinskaitė ◽  
Elicija Stackevičienė ◽  
Mindaugas Liaudanskas ◽  
Kristina Zymonė ◽  
Vaidotas Žvikas ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vaccinium Macrocarpon ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
American Cranberry ◽  
Fruit Samples ◽  
Phytochemical Profile ◽  
Ethanol Extracts ◽  
The Individual

The aim of this study was to determine the composition and content of phenolic compounds in ethanol extracts of eight different cultivars of American cranberry (Vaccinium macrocarpon Aiton) fruit using spectrophotometric and UPLC-ESI-MS/MS analysis and to evaluate the antioxidant activity in vitro of these extracts. The highest total amount of phenolic compounds evaluated via Folin–Ciocalteu spectrophotometry was detected in American cranberry fruit samples of the ‘Bain’ clone, and the highest total amount of flavonoids was found in samples of the ‘Drever’ and ‘Baiwfay’ cultivars. The highest total amount of the individual phenolic compounds (519.53 ± 25.12 mg/g DW) identified and quantitatively evaluated via chromatography was detected in samples of the ‘Searles’ cranberry cultivar. In the studied cranberry samples, the predominant phenolic compounds were hyperoside, quercetin, and procyanidin A2, while the amounts of other compounds were significantly lower. HCA and PCA revealed that ‘Woolman’, ‘Holliston’, ‘Pilgrim, and ‘Searles’ fruit samples had different quantitative content of phenolic compounds from other cranberry cultivars. Meanwhile, fruit of ‘Baiwfay’, ‘Drever’, ‘Bain’, and ‘Bergman’ were similar in their phytochemical profile.

In vitro Propagation and Chemical and Biological Studies of the Essential Oil of Salvia przewalskii Maxim

2007 ◽  
Vol 62 (11-12) ◽  
pp. 839-848 ◽  
Author(s):  
Ewa Skała ◽  
Danuta Kalemba ◽  
Anna Wajs ◽  
Marek Róźalski ◽  
Urszula Krajewska ◽  
...  
Keyword(s):  
Essential Oil ◽  
Shoot Multiplication ◽  
Quantitative Composition ◽  
Human Leukemia ◽  
Qualitative And Quantitative ◽  
H Nmr ◽  
Regenerated Plants ◽  
Biological Studies

The procedure of Salvia przewalskii shoot multiplication and the ability of regenerated plants to produce essential oil is reported. The essential oil was obtained by hydrodistillation from leaves and flowering stems of field-grown plants, and their chemical composition was examined by GC, GC-MS and 1H NMR. The differences in yield as well as qualitative and quantitative composition between the oils isolated from in vitro and in vivo plants were observed. S. przewalskii essential oil was tested for its antimicrobial and cytotoxic properties. It was found that cytotoxicity against human leukemia HL-60 cells and antimicrobial activity (especially, against Staphylococcus aureus and S. epidermidis strains) of oils isolated from in vitro plants were higher than those for oils from in vivo S. przewalskii plants.

scholarly journals Comparative Analysis of Therapeutically Important Indole Compounds in in vitro Cultures of Hypericum perforatum Cultivars by HPLC and TLC Analysis Coupled with Densitometric Detection

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Bożena Muszyńska ◽  
Halina Ekiert ◽  
Inga Kwiecień ◽  
Anna Maślanka ◽  
Rawad Zodi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Hypericum Perforatum ◽  
Dry Mass ◽  
In Vitro Cultures ◽  
Indole Compounds ◽  
Densitometric Detection ◽  
Tlc Analysis

Five indole compounds (5-hydroxy-L-tryptophan, L-tryptophan, indole-3-acetic acid, melatonin, serotonin) and hypericin were identified and quantified in methanolic extracts of shoot cultures of three Hypericum perforatum cultivars (Helos, Elixir, Topas) growing on two variants of Murashige -Skoog medium differing in concentrations of growth regulators (naphthalene-1-acetic acid and 6-benzylaminopurine). Extracts of the aboveground parts of field-grown plants ( Hyperici herba) were also analyzed by HPLC and TLC analysis coupled with densitometric detection. Determination of four compounds was based on our assay described earlier. The methods of determination of 5-hydroxy-L-tryptophan and hypericin were developed and validated in this study. The composition and contents of the metabolites under study differed between the cultivars cultured in vitro and between medium variants containing diverse contents of growth regulators. The contents of individual indole compounds in the biomass from in vitro cultures ranged from 39.6 to 343.2 mg/100 g dry mass. 5-Hydroxy-L-tryptophan was the dominating metabolite (from 78.2 to 343.2 mg/100 g dry mass). Extracts from shoots of the cultivar Helos also contained high contents of serotonin (319.9 and 197.4 mg/100 g dry mass). The contents of indole compounds in Hyperici herba were also diverse (from 7.1 to 55.3 mg/100 g dry mass). 5-Hydroxy-L-tryptophan was the dominating metabolite as well. Hypericin content of Hyperici herba, equaling 12.2 mg/100 g dry mass was from 3.3 to 10 times higher than in extracts from shoots cultured in vitro. The present report is the first analysis of endogenous accumulation of indole compounds in Hyperici herba which involves, apart from melatonin, four other compounds.

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