scholarly journals Variability in the Qualitative and Quantitative Composition and Content of Phenolic Compounds in the Fruit of Introduced American Cranberry (Vaccinium macrocarpon Aiton)

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1379
Author(s):  
Ieva Gudžinskaitė ◽  
Elicija Stackevičienė ◽  
Mindaugas Liaudanskas ◽  
Kristina Zymonė ◽  
Vaidotas Žvikas ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Vaccinium Macrocarpon ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
American Cranberry ◽  
Fruit Samples ◽  
Phytochemical Profile ◽  
Ethanol Extracts ◽  
The Individual

The aim of this study was to determine the composition and content of phenolic compounds in ethanol extracts of eight different cultivars of American cranberry (Vaccinium macrocarpon Aiton) fruit using spectrophotometric and UPLC-ESI-MS/MS analysis and to evaluate the antioxidant activity in vitro of these extracts. The highest total amount of phenolic compounds evaluated via Folin–Ciocalteu spectrophotometry was detected in American cranberry fruit samples of the ‘Bain’ clone, and the highest total amount of flavonoids was found in samples of the ‘Drever’ and ‘Baiwfay’ cultivars. The highest total amount of the individual phenolic compounds (519.53 ± 25.12 mg/g DW) identified and quantitatively evaluated via chromatography was detected in samples of the ‘Searles’ cranberry cultivar. In the studied cranberry samples, the predominant phenolic compounds were hyperoside, quercetin, and procyanidin A2, while the amounts of other compounds were significantly lower. HCA and PCA revealed that ‘Woolman’, ‘Holliston’, ‘Pilgrim, and ‘Searles’ fruit samples had different quantitative content of phenolic compounds from other cranberry cultivars. Meanwhile, fruit of ‘Baiwfay’, ‘Drever’, ‘Bain’, and ‘Bergman’ were similar in their phytochemical profile.

scholarly journals Development, Validation, and Application of the UPLC-DAD Methodology for the Evaluation of the Qualitative and Quantitative Composition of Phenolic Compounds in the Fruit of American Cranberry (Vaccinium macrocarpon Aiton)

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 467
Author(s):  
Rima Urbstaite ◽  
Lina Raudone ◽  
Mindaugas Liaudanskas ◽  
Valdimaras Janulis
Keyword(s):  
Phenolic Compounds ◽  
Biological Effects ◽  
Cost Effective ◽  
Vaccinium Macrocarpon ◽  
Raw Material ◽  
Quantitative Composition ◽  
Quercetin Derivatives ◽  
Qualitative And Quantitative ◽  
American Cranberry ◽  
Fruit Samples

Phenolic compounds in the fruit of American cranberry (Vaccinium macrocarpon Aiton) determine the antioxidant, anti-inflammatory, anticancer, and other biological effects. The berries are used in the production of medicinal preparations and food supplements, which highlights the importance of qualitative and quantitative analysis of phenolic compounds in cranberry fruit raw material. The aim of our study was to develop and validate an efficient, cost-effective, reproducible, and fast UPLC-DAD methodology for the evaluation of the qualitative and quantitative composition of phenolic compounds in raw material and preparations of American cranberry fruit. During the development of the methodology, chlorogenic acid and the following flavonols were identified in cranberry fruit samples: myricetin-3-galactoside, quercetin-3-galactoside, quercetin-3-glucoside, quercetin-3-α-L-arabinopyranoside, quercetin-3-α-L-arabinofuranoside, quercetin-3-rhamnoside, myricetin, and quercetin. The developed and optimized UPLC-DAD methodology was validated according to the guidelines of the International Council for Harmonization (ICH), evaluating the following parameters: range, specificity, linearity (R2 > 0.999), precision (%RSD < 2%), LOD (0.38–1.01 µg/mL), LOQ (0.54–3.06 µg/mL), and recovery (80–110%). The developed methodology was applied to evaluate the qualitative and quantitative composition of phenolic compounds in fruit samples of cranberry cultivars ‘Baifay’, ‘Bergman’, ‘Prolific’, and ‘Searles’, as well as ‘Bain-MC’ and ‘BL-12′ clones. In the tested samples, the majority (about 70%) of the identified flavonols were quercetin derivatives. The greatest amount of quercetin-3-galactoside (1035.35 ± 4.26 µg/g DW) was found in fruit samples of the ‘Searles’ cultivar, and the greatest amount of myricetin-3-galactoside (940.06 ± 24.91 µg/g DW) was detected in fruit samples of the ‘Woolman’ cultivar.

scholarly journals Composition and Antioxidant Activity of Phenolic Compounds in Fruit of the Genus Rosa L.

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 545
Author(s):  
Mindaugas Liaudanskas ◽  
Irena Noreikienė ◽  
Kristina Zymonė ◽  
Rugilė Juodytė ◽  
Vaidotas Žvikas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Content ◽  
Quantitative Composition ◽  
Rosa Multiflora ◽  
Epicatechin Gallate ◽  
Qualitative And Quantitative ◽  
Fruit Samples

We investigated the qualitative and quantitative composition of phenolic compounds in the fruit of Rosa L. cultivars grown in Lithuania. The highest total content of phenolic compounds (50.13 ± 4.17 mg GAE/g, p < 0.05) was determined in fruit samples of Rosa pimpinellifolia L. cultivar “Single Cherry”. The highest levels of hydroxycinnamic acid derivatives were determined in fruit samples of Rosa rugosa Thunb. cultivars “Dart’s Defender” and “Adam Chodun”. The highest flavonoid content was determined in fruit samples of Rosa multiflora Thunb. cultivar “Nana” and R. multiflora species. The strongest antioxidant activity evaluated by applying DPPH and FRAP assays was determined in fruit extracts of R. pimpinellifolia cultivar “Single Cherry” and R. rugosa cultivar “Adam Chodun”. Qualitative and quantitative analysis of phenolic compounds in Rosa L. fruit was performed by applying UHPLC. The following phenolic compounds were identified in fruit samples: caffeic acid, chlorogenic acid, quercetin, quercitrin, (+)-catechin, (−)-epicatechin, (−)-epicatechin gallate, rutin, phloridzin, and kaempferol-3-O-glycoside. A strong correlation was determined between the total amount of phenolic compounds determined in extracts of the fruit samples of Rosa L. cultivars and the radical scavenging and reducing activity of their extracts in vitro (R = 0.767 and 0.727, respectively, p < 0.05).

scholarly journals The role of poliphenols in formation of fungicidal activity and resistance to exometabolites of the fungus on the example of in vitro crops sunflower and savory

2021 ◽  
Vol 40 ◽  
pp. 01014
Author(s):  
Elena Kalashnikova ◽  
Svetlana Zaytseva ◽  
Nguen Tan Thay ◽  
Rima Kirakosyan ◽  
Mikhail Cherednichenko ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Fungicidal Activity ◽  
Sharp Decrease ◽  
Callus Cultures ◽  
Biosynthetic Activity ◽  
Qualitative And Quantitative ◽  
Ethanol Extracts

When studying the formation of secondary metabolites represented by phenolic compounds in callus cultures of sunflower plants, it was found that under in vitro conditions under the influence of a stress factor, the accumulation of polyphenols increases. It was found that the biosynthetic activity of callus cultures with respect to polyphenol increases in proportion to the concentration of the culture filtrate of the fungus (Sclerotinia Sclerotiorum) in the nutrient medium. This is confirmed by the qualitative and quantitative determination of the content of polyphenols in callus cultures. In savory plants, when introduced into culture in vitro, there is a sharp decrease in the biosynthesis of phenolic compounds. Ethanol extracts of microclones and callus cultures showed no fungicidal activity.

scholarly journals The Qualitative and Quantitative Compositions of Phenolic Compounds in Fruits of Lithuanian Heirloom Apple Cultivars

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5263
Author(s):  
Aurita Butkevičiūtė ◽  
Mindaugas Liaudanskas ◽  
Darius Kviklys ◽  
Dalia Gelvonauskienė ◽  
Valdimaras Janulis
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Apple Fruit ◽  
Hplc Method ◽  
Quantitative Composition ◽  
Qualitative And Quantitative ◽  
Fruit Samples ◽  
Biological Compounds ◽  
Apple Cultivars ◽  
Processed Products

As the interest in heirloom cultivars of apple trees, their fruit, and processed products is growing worldwide, studies of the qualitative and quantitative composition of biological compounds are important for the evaluation of the quality and nutritional properties of the apples. Studies on the variations in the chemical composition of phenolic compounds characterized by a versatile biological effect are important when researching the genetic heritage of the heirloom cultivars in order to increase the cultivation of such cultivars in orchards. A variation in the qualitative and quantitative composition of phenolic compounds was found in apple samples of cultivars included in the Lithuanian collection of genetic resources. By the high-performance liquid chromatography (HPLC) method flavan-3-ols (procyanidin B1, procyanidin B2, procyanidin C2, (+)-catechin and (−)-epicatechin), flavonols (rutin, hyperoside, quercitrin, isoquercitrin, reynoutrin and avicularin), chlorogenic acids and phloridzin were identified and quantified in fruit samples of heirloom apple cultivars grown in Lithuania. The highest sum of the identified phenolic compounds (3.82 ± 0.53 mg/g) was found in apple fruit samples of the ‘Koštelė’ cultivar

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Antioxidant activities and A549 lung adenocarcinomic cells against capacity from various extracts of Agrimonia pilosa Ledeb

2018 ◽  
Vol 4 (3) ◽  
pp. 114-119
Author(s):  
Quang Ung Le ◽  
◽  
Horng Liang Lay ◽  
Ming Chang Wu ◽  
◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
A549 Cells ◽  
Total Phenolic ◽  
Root Extract ◽  
Inhibitory Capacity ◽  
Ethanol Extracts

Agrimonia pilosa Ledeb (AL) has received considerable attention as a herbal medicine for its applications in ethnopharmacology with heath benefits. This study aimed to investigate antioxidant activities and A549 growth inhibitory capacity from its root extract (RE) and aerial parts extract (AE). The 50% ethanol extracts were used for the tests. The total polyphenol content and the antioxidant effects comprising ABTS+ and DPPH free radical scavenging activities were evaluated. Phenolic compounds in the extracts were isolated using high performance liquid chromatography (HPLC). Lactate dehydrogenase released in medium was also evaluated. Total phenolic and flavonoid content, and in vitro antioxidant potential of the RE were higher (p<0.01) than that of the AE. Two individual phenolic compounds consisting of 4-hydroxybenzoic acid and p-coumaric acid were firstly identified in both by HPLC. The RE exhibited higher A549 inhibitory capacity compared to the AE and activated the apoptotic proteins of bcl-2, bax, bad, caspase-3 and caspase-9 in A549. In conclusion, the AL extracts were more effective in antioxidant and A549 cells inhibitory capacity.

In vitro Propagation and Chemical and Biological Studies of the Essential Oil of Salvia przewalskii Maxim

2007 ◽  
Vol 62 (11-12) ◽  
pp. 839-848 ◽  
Author(s):  
Ewa Skała ◽  
Danuta Kalemba ◽  
Anna Wajs ◽  
Marek Róźalski ◽  
Urszula Krajewska ◽  
...  
Keyword(s):  
Essential Oil ◽  
Shoot Multiplication ◽  
Quantitative Composition ◽  
Human Leukemia ◽  
Qualitative And Quantitative ◽  
H Nmr ◽  
Regenerated Plants ◽  
Biological Studies

The procedure of Salvia przewalskii shoot multiplication and the ability of regenerated plants to produce essential oil is reported. The essential oil was obtained by hydrodistillation from leaves and flowering stems of field-grown plants, and their chemical composition was examined by GC, GC-MS and 1H NMR. The differences in yield as well as qualitative and quantitative composition between the oils isolated from in vitro and in vivo plants were observed. S. przewalskii essential oil was tested for its antimicrobial and cytotoxic properties. It was found that cytotoxicity against human leukemia HL-60 cells and antimicrobial activity (especially, against Staphylococcus aureus and S. epidermidis strains) of oils isolated from in vitro plants were higher than those for oils from in vivo S. przewalskii plants.

Antibacterial activity of isolated phenolic compounds from cranberry (Vaccinium macrocarpon) against Escherichia coli

2016 ◽  
Vol 7 (3) ◽  
pp. 1564-1573 ◽  
Author(s):  
Celia Rodríguez-Pérez ◽  
Rosa Quirantes-Piné ◽  
José Uberos ◽  
Cecilia Jiménez-Sánchez ◽  
Alejandro Peña ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Surface Hydrophobicity ◽  
Vaccinium Macrocarpon ◽  
E Coli

Apart from proanthocyanidins, isolated polyphenols from cranberry can act againstE. coliadherence and/or modify its surface hydrophobicityin vitro.

scholarly journals Determination of Quality Characteristics, Phenolic Compounds and Antioxidant Activity of Propolis from Southeastern Mexico

2021 ◽  
Vol 65 (1) ◽  
pp. 109-122
Author(s):  
Enrique Sauri-Duch ◽  
Cesia Gutiérrez-Canul ◽  
Luis F. Cuevas-Glory ◽  
Lorena Ramón-Canul ◽  
Emilio Pérez-Pacheco ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Geographical Location ◽  
Principal Component ◽  
Total Phenolic ◽  
Dry Extract ◽  
Extract Content ◽  
The Individual

Abstract The objective of this work was to investigate the variability of physicochemical parameters, phenolic compounds and in vitro antioxidant activity of propolis collected from different apiaries in southeastern Mexico. A high variability was found in the moisture content (1.96–8.26%), ash (0.66–5.50%) and sensory characteristics of raw propolis from southeastern Mexico, but the raw propolis samples met the requirements of the quality regulations. In the same way, most of the ethanolic extracts also complied with the quality regulations. Of all the extracts, PE2 obtained from Santa Cruz showed the highest values for dry extract, content of total phenolic compounds (TPC), total flavonoids (TF) and antioxidant activity (DPPH and ABTS). The content of the individual phenolic compounds varied according to the geographical location of the apiary, but the PE2 extract resulted in the highest pinocembrin and chrysin content. A positive correlation was obtained between TPC and TF with antioxidant activity. Propolis extracts were classified into two groups through principal component analysis (PCA). These results indicate that the apiary location in southeastern Mexico influenced the characteristics of propolis.

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