scholarly journals Hygienisation of Surplus Activated Sludge by Hydrodynamic Cavitation

2012 ◽  
Vol 19 (3) ◽  
pp. 415-421
Author(s):  
Alicja Machnicka ◽  
Klaudiusz Grűbel ◽  
Agnieszka Rusin
Keyword(s):  
Activated Sludge ◽  
Pathogenic Bacteria ◽  
Bacterial Culture ◽  
Hydrodynamic Cavitation ◽  
Chemical Methods ◽  
The Family ◽  
Before And After ◽  
Microbiological Analyses ◽  
Number Of Bacteria ◽  
Surrounding Liquid

Hygienisation of Surplus Activated Sludge by Hydrodynamic CavitationThe success of an effective process of disintegration is the application of physical or chemical methods of the destruction of the cell wall which consequently becomes fragmented and the intracellular matters are released into the surrounding liquid. The purpose of the conducted experiments was to demonstrate possibilities of application of the hydrodynamic in the bacteriological hygienisation of surplus activated sludge. The confirmation of the effectiveness of the bactericidal activity of hydrodynamic cavitation was the number of bacteria grown on agars before and after hygienisation process. Selective and basic beddings have been used in the research. The bacterial culture has lasted approximately 24 to 48 hours. After the period of incubation the colonies produced have been counted and initially identified. Based on the microbiological analyses a significant reduction in an overall number of bacteria and pathogenic bacteria belonging to the familyEnterobacteriaceaeand to the pathogenic speciesStaphylococcushas been noticed. The overall number of bacteria decrease about 80% after 45 minutes of disintegration of surplus activated sludge. Pathogenic bacteria belonging to the familyEnterobacteriaceae: lactose-positive and lactose-negative decrease about 93% and 92%, respectively. Reducing the number ofSalmonellabacteria was 100% after 45 minutes of disintegration processes. Reduction bacteria of speciesStaphylococcuswere about 93% for mannitolo-positive and 92% for mannitolo-negative.

scholarly journals Hygienization of Surplus Activated Sludge By Dry Ice

2015 ◽  
Vol 21 (4) ◽  
pp. 651-660 ◽  
Author(s):  
Ewelina Nowicka ◽  
Alicja Machnicka
Keyword(s):  
Organic Matter ◽  
Activated Sludge ◽  
Pathogenic Bacteria ◽  
Destructive Effect ◽  
Dry Ice ◽  
Factors Affecting ◽  
Supernatant Liquid ◽  
Pathogenic Microbes ◽  
Microbiological Analyses ◽  
Number Of Bacteria

Abstract The presence of pathogenic microorganisms in sewage sludge is one of the crucial factors affecting their use in agriculture and in nature. An appropriate method of sludge hygienization can contribute to the reduction of the presence of pathogenic microbes in the sludge or to their complete elimination. This study presents the possibility of using dry ice in the hygienization of surplus activated sludge. The scope of survey covered microbiological as well as physiochemical analyses of the sludge. The microbiological analyses conducted show the hygienization effect of dry ice on surplus activated sludge such as the reduction of the total number of bacteria as well as of pathogenic bacteria. In the case of sludge to dry ice 1:1 voluminal ratio, the total number of bacteria has been reduced by 76%, the number of Salmonella sp. rods by 90%, and Clostridium perfringens by 85%. The results of physiochemical analyses confirmed the destructive effect that dry ice has over microorganisms. The disintegration of microbes by means of dry ice contributed to the increase of supernatant liquid turbidity and to the release of organic matter. For the sludge to dry ice 1:1 voluminal ratio, an increase in turbidity of supernatant liquid by 320 mg SiO2 dm-3 has been proven, as well as an increase in the value of CODCr by 549 mg O2 dm-3, which indicates the release of organic matter into the liquid phase.

APPLICATION OF THE ATP-BIOLUMINESCENCE METHOD TO ASSESS THE QUALITY OF SANITATION OF MILKING EQUIPMENT

2020 ◽  
Vol 1 (4) ◽  
pp. 468-472
Author(s):  
P.A. Popov ◽  
◽  
D.V. Gruznov ◽  
S.V. Tokarev ◽  
◽  
...  
Keyword(s):  
Dairy Farms ◽  
Laboratory Studies ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Microbial Number ◽  
Proportional Relationship ◽  
The Relationship ◽  
Number Of Bacteria ◽  
Bioluminescence Method

The article presents the results of determining the relationship between the total microbial number and microbial ATP on the surface of milking equipment before and after treatment with neutral anolyte ANK-SUPER. The possibility of using the ATP-bioluminescence method to control the quality of sanitation of milking equipment on dairy farms is shown. Laboratory studies revealed a proportional relationship between the level of bacterial ATP and the number of bacteria on the surface of milking equipment before and after sanitation.

scholarly journals Interactions between Rainbow Trout Eyed Eggs and Flavobacterium spp. Using a Bath Challenge Model: Preliminary Evaluation of Bacteriophages as Pathogen Control Agents

2021 ◽  
Vol 9 (5) ◽  
pp. 971
Author(s):  
Valentina L. Donati ◽  
Inger Dalsgaard ◽  
Anniina Runtuvuori-Salmela ◽  
Heidi Kunttu ◽  
Johanna Jørgensen ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Pathogenic Bacteria ◽  
Infection Model ◽  
Preliminary Evaluation ◽  
Fish Eggs ◽  
Short Term ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Pathogen Control ◽  
Number Of Bacteria ◽  
Bath Challenge

The microbial community surrounding fish eyed eggs can harbor pathogenic bacteria. In this study we focused on rainbow trout (Oncorhynchus mykiss) eyed eggs and the potential of bacteriophages against the pathogenic bacteria Flavobacterium psychrophilum and F. columnare. An infection bath method was first established, and the effects of singular phages on fish eggs was assessed (survival of eyed eggs, interaction of phages with eyed eggs). Subsequently, bacteria-challenged eyed eggs were exposed to phages to evaluate their effects in controlling the bacterial population. Culture-based methods were used to enumerate the number of bacteria and/or phages associated with eyed eggs and in the surrounding environment. The results of the study showed that, with our infection model, it was possible to re-isolate F. psychrophilum associated with eyed eggs after the infection procedure, without affecting the survival of the eggs in the short term. However, this was not possible for F. columnare, as this bacterium grows at higher temperatures than the ones recommended for incubation of rainbow trout eyed eggs. Bacteriophages do not appear to negatively affect the survival of rainbow trout eyed eggs and they do not seem to strongly adhere to the surface of eyed eggs either. Finally, the results demonstrated a strong potential for short term (24 h) phage control of F. psychrophilum. However, further studies are needed to explore if phage control can be maintained for a longer period and to further elucidate the mechanisms of interactions between Flavobacteria and their phages in association with fish eggs.

scholarly journals The impact of a ‘milking the COW’ campaign in a regional hospital in Singapore

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Surinder Kaur M. S. Pada ◽  
Poh Lishi ◽  
Kim Sim Ng ◽  
Sarathamani Rethenam ◽  
Lilibeth Silagan Alenton ◽  
...  
Keyword(s):  
Hand Hygiene ◽  
Pathogenic Bacteria ◽  
Mixed Model ◽  
Mixed Model Analysis ◽  
Colony Forming Units ◽  
Skin Flora ◽  
Before And After ◽  
The Difference ◽  
And Control ◽  
The Impact

Abstract Background Computerisation of various processes in hospitals and reliance on electronic devices raises the concern of contamination of these devices from the patient environment. We undertook this study to determine if an attached hand hygiene device that unlocks the screen of a computer on wheels (COW) on usage can be effective in decreasing the microbiological burden on computer keyboards. Methods An electronic hand sanitizer was integrated onto the COW. A prospective cohort study with a crossover design involving 2 control and 2 intervention wards was used. The study end point was the number of colony forming units found on the keyboards. Bacteria were classified into 4 main groups; pathogenic, skin flora, from the environment or those thought to be commensals in healthy individuals. We then used a mixed effects model for the statistical analysis to determine if there were any differences before and after the intervention. Results Thirty-nine keyboards were swabbed at baseline, day 7 and 14, with 234 keyboards cultured, colony forming units (CFUs) counted and organisms isolated. By mixed model analysis, the difference of mean bacteria count between intervention and control for week 1 was 32.74 (− 32.74, CI − 94.29 to 28.75, p = 0.29), for week 2 by 155.86 (− 155.86, CI − 227.45 to − 83.53, p < 0.0001), and after the 2-week period by 157.04 (− 157.04, CI − 231.53 to − 82.67, p < 0.0001). In the sub-analysis, there were significant differences of pathogenic bacteria counts for the Intervention as compared to the Control in contrast with commensal counts. Conclusion A hand hygiene device attached to a COW may be effective in decreasing the microbiological burden on computer keyboards.

scholarly journals Produção das equipes da Estratégia Saúde da Família antes e após a implantação do PMAQ-AB / Production of the Family Health Strategy teams before and after the implementation of PMAQ-AB

2018 ◽  
Vol 16 (3) ◽  
Author(s):  
Selma Regina de Andrade ◽  
Renata Goulart Castro ◽  
Monique Haenscke Senna
Keyword(s):  
Family Health ◽  
Health Strategy ◽  
The Family ◽  
Before And After ◽  
Family Health Strategy

Estudo com o objetivo de analisar a produção das equipes da Estratégia de Saúde da Família, com base em indicadores do Sistema de Informação da Atenção Básica, antes e após a implantação do Programa para Melhoria da Qualidade na Atenção Básica. Utilizou-se abordagem quantitativa, transversal, descritiva e exploratória, a partir dos Relatórios da Situação de Saúde e Acompanhamento das Famílias, referentes às equipes. Foram incluídas 112 equipes divididas em dois grupos. Realizou-se análise descritiva do conjunto de indicadores e procedeu-se a estatística exploratória e analítica para comparar as diferenças entre os grupos. Constatou-se que as equipes, independentemente de aderirem ou não ao programa, obtiveram incremento nos registros do sistema de informação, sinalizando uma sensibilização de toda a rede para melhoria dos seus registros. Apesar deste aumento, o acompanhamento das famílias não ocorreu na mesma proporção que os cadastramentos.

scholarly journals Эколого-биологическая характеристика Lactobacillus acidophilus

2017 ◽  
Vol 7 (4) ◽  
pp. 214-230 ◽  
Author(s):  
A. N. Irkitova ◽  
A. V. Matsyura
Keyword(s):  
Lactobacillus Acidophilus ◽  
Pathogenic Bacteria ◽  
Molecular Mechanisms ◽  
Systematic Position ◽  
New Class ◽  
Safe Level ◽  
Molecular Approaches ◽  
The Family ◽  
Long Time ◽  
Biochemical Identification

<p>Lactobacillus acidophilus - homofermentative lactobacillus, specializing in living in the gastrointestinal and urogenital tracts of mammals and birds. It accompanies a person from birth and throughout his life, providing a whole range of useful services, the main one of which is active participation in the body's defense system against the harmful action of undesirable microorganisms (preventing the growth of pathogenic bacteria and restraining populations of opportunistic microbes at a safe level) . It is this property of the acidophilus rod that explains its wide practical use in various probiotic products and preparations of dietary, medical and agricultural purposes.<br />Although the acidophilus rod is known and purposefully used for a long time, it still ha the great potential for the research. The use of gene-molecular approaches has made it possible to clarify the systematic position of L. acidophilus in the family of lactobacilli and to identify a group of closely related species, often indistinguishable by traditional physiological and biochemical identification methods. Today, the efforts of researchers are focused on elucidating the molecular mechanisms by which antagonistically active strains of L. acidophilus carry out a bactericidal and bacteriolytic effect on harmful microbes. Disclosure of these mechanisms will not only allow more efficient selection and use of strains of L. acidophilus, but also create a new class of antibiotics that are more effective and have less side effects than existing ones.<br />This review is devoted to the description of the probiotic microorganism Lactobacillus acidophilus. In the article the biological and ecological properties of the acidophilus rod are described in detail, examples of practical use of this microorganism in various branches of the national economy are given.</p>

scholarly journals Analysis of Nursing Clinical Practice of Oral Hygiene on Patient with Hemorraghic Stroke – A Case Study

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Yuyun Durhayati ◽  
Cut Sarida Pompey
Keyword(s):  
Oral Hygiene ◽  
Assessment Tool ◽  
Health Assessment ◽  
Hygiene Intervention ◽  
The Family ◽  
Before And After ◽  
Nursing Clinical Practice ◽  
Nursing Clinical ◽  
Neurological Ward

<p><strong>Objective</strong>: The  purpose  of  this  case  study  was  to  analyze  the  oral  hygiene intervention performed by  nurse  in  stroke  hemorrhagic  patient  in  neurological  ward</p><p> </p><p><strong>Methods:</strong> .Oral hygiene interventions were performed by using 0.2% chlorhexidine twice daily for seven days. This case study used oral health assessment tool (OHAT) which the measurements were taken before and after the intervention every day</p><p> </p><p><strong>Results</strong>: There was a decrease in oral hygiene assessment score from 9 to 1 which implied an improvement in oral hygiene condition. This study also showed that family of stroke patient was able to perform oral hygiene practice to the patient after simulation performed firstly by nurses</p><p> </p><p><strong>Conclusion</strong>: The study recommends that oral hygiene by using 0.2% chlorhexidine needs to be delivered routinely by nurses and continues by the family at home care.</p><p> </p><p><strong>Keywords</strong>: chlorhexidine 0.2%, oral hygiene, stroke.</p>

scholarly journals PSYCHOLOGICAL AND PEDAGOGICAL SUPPORT FOR THE FAMILY WITH YOUNGER SCHOOLBOY WITH MENTAL RETARDATION

2020 ◽  
pp. 178-185
Author(s):  
G. Yu. Taskina ◽  
I. A. Taskina
Keyword(s):  
Mental Retardation ◽  
Primary School ◽  
School Age ◽  
Modern Education ◽  
Parents And Children ◽  
The Family ◽  
Before And After ◽  
Primary School Age

An urgent issue of modern education – the issue of psychological and pedagogical support for the family with a child with health disabilities has been considered. It has been specified, that particularly specially organized work can help parents and members of family with a child with health disabilities to cope with the difficulties of raising child and carry out his socialization. In particular, the results of a study of the personality of the parents of a child of primary school age with a mental retardation, their chosen style of child upbringing, the nature of interaction with the child have been presented. In addition, the effectiveness of the compiled and tested program of psychological and pedagogical support for the family with a child with a mental retardation, changes in the behavior of parents and children before and after testing the program have been analysed.

scholarly journals UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

2018 ◽  
Vol 7 (2) ◽  
pp. 125-139
Author(s):  
Thais Nogueira Gonzaga ◽  
Dora Inés Kozusny-Andreani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Health Care Services ◽  
Pathogenic Bacteria ◽  
Ozone Treatment ◽  
Microbiological Analysis ◽  
Before And After ◽  
Staphylococcus Spp

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.Palavras-chave: Bactérias patogênicas. Fungos. Ozonização. USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.Keywords: Pathogenic bacteria. Fungi. Ozonization.

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