scholarly journals PROFIL BIOAUTOGRAFI DAN UJI PENANGKAP RADIKAL 2,2-DIFENIL-1-PIKRIHIDRAZIL OLEH EKSTRAK ETANOL DAUN BINAHONG (Anredera scandens (L.) Moq.) DAN FRAKSI-FRAKSINYA

Jurnal Kimia ◽  
2020 ◽  
pp. 10
Author(s):  
P. O. Samirana ◽  
D. A. Swastini ◽  
A. A. G. R. Y. Putra ◽  
I P. W. Kusuma ◽  
N. P. A. Y. Pratiwi ◽  
...  
Keyword(s):  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Extraction Process ◽  
Scientific Activity ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Chloroform Fraction ◽  
Ic50 Values

Free radicals are atoms or molecules that have one or more unpaired electrons and are highly reactive. Antioxidants can inhibit the excessive oxidation reaction. The leaves of binahong (Anredera scandens (L.) Moq.) are one part of plants which contain flavonoid compounds and tannins that have scientific activity as antioxidants.This study aims to determine the bioautographic profile and to test the antioxidant activity of the ethanol extract of the leaves of Anredera scandens (L.) Moq. useing 2.2-diphenyl-1-pikrilhidrazil radical capture method. The extraction process produced 19.22% yield, 14.68% (14.684 grams) chloroform fraction, 11.22% (11.224 grams) n-hexane fraction, 3.09% (3.094 grams) ethyl acetate fraction, and 23.90% (23.904 grams) n-butanol fraction. The results of bioautographic profile test using KLT densitometry method proved that ethanol extract, chloroform fraction and ethyl acetate fraction have DPPH radical capture activities. The determination of total flavonoid content found that ethyl acetate fraction contained higher total flavonoids than other fractions. In the ethyl acetate fraction, high total flavonoid level and low IC50 values were obtained, while the n-hexane fraction had low flavonoid level and high IC50 values.   Keywords: antioxidants, binahong leaves, free radicals, flavonoids, IC50.

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

2019 ◽  
pp. 8-14
Author(s):  
Risa Supringrum ◽  
Siti Jubaidah
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Hexane Fraction ◽  
High Dose ◽  
Water Fraction ◽  
Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

scholarly journals Identification of Active Compound Extracts from Noni Fruit (Morinda citrifolia L.) and Its Potential as Antioxidants

2019 ◽  
Vol 22 (5) ◽  
pp. 206-212
Author(s):  
Sogandi Sogandi ◽  
Rabima Rabima
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Morinda Citrifolia ◽  
Chloroform Fraction ◽  
Oxidation Reactions ◽  
First Time

Antioxidants are compounds that can inhibit oxidation reactions by binding to free radicals and highly reactive molecules. One form of reactive oxygen compounds is free radicals, these compounds are formed in the body and are triggered by various factors. Noni fruits have been used by the community as a medicine for high blood pressure, beriberi, urinating, inflammation of bile, inflammation of the intestine, dysentery, constipation, spleen pain, liver pain, diabetes and lumbago. Nevertheless, research on the identification of the active compounds of noni fruit which act as antioxidants yet. This study aims to extract and fractionation of Noni fruit, determine the antioxidant activity of Noni fruit extract, and identify the types of bioactive compounds that act as antioxidants. Noni fruit was extracted used maceration technique with 96% ethanol and then fractionated using n-hexane, chloroform and ethyl acetate solvents. Antioxidant activity from the results of noni fruit fractionation was measured by the DPPH (1.1 diphenyl-2-picrylhydrachyl) method and identification of bioactive compounds was carried out by GCMS. The results showed the greatest antioxidant activity was found in chloroform fraction with a percentage of inhibition of 78.19%. For the first time this study revealed the types of bioactive compounds from noni fruit ethyl acetate fraction which act as antioxidants are n-hexadecanoic acid, squalene, pyridin-3-carboxamide, oxime, n- (2-trifluoro methyl phenyl), and beta-sitosterol

scholarly journals THE ANTIRHEUMATIC ACTIVITY OF MUNTINGIA CALABURA L. LEAVES ETHANOL EXTRACT AND ITS FRACTION

2016 ◽  
Vol 10 (1) ◽  
pp. 84
Author(s):  
Jamilah Sarimanah ◽  
Ketut Adnyana I ◽  
Elin Yulinah Sukandar ◽  
Neng Fisheri Kurniati
Keyword(s):  
Ethyl Acetate ◽  
Chronic Inflammation ◽  
Ethanol Extract ◽  
Inflammatory Cells ◽  
Ethyl Acetate Fraction ◽  
Cartilage Destruction ◽  
Hexane Fraction ◽  
Intraplantar Injection ◽  
The Right ◽  
Muntingia Calabura

ABSTRACTObjective: Rheumatoid arthritis is chronic inflammation disorder that can be treated with anti-inflammatory drug such as methylprednisolone.Several medicinal plants shave the potential to be used in arthritis therapy, one of which is Muntingia calabura L. The objective of this research wasto study the antirheumatic activity of M. calabura leaves ethanol extract and its n-hexane, ethyl acetate, and water fractions in a rheumatoid arthriticrat model.Methods: Rheumatoid was induced in rats by intraplantar injection of Complete Freund’s Adjuvant (CFA) into the right foot paw of Wistar rats. Thepaw volume was measured by a plethysmometer. The histology of right foot paw was carried out. For chronic CFA inflammation, induced paw edemaassay was used, whereas chronic inflammation was assessed by CFA induced arthritis. The histopathology of synovial joints (paw) was carried out.Result: The results of the study revealed that in CFA-induced arthritic rats, methylprednisolone, M. calabura ethanol extract, and its fractions wereable to reduce inflammation. Histopathology examination also showed a reduction of in cartilage destruction, influx of inflammatory cells, pannusformation, fibrin deposition, and synovitis.Conclusion: M. calabura L. n-hexane fraction showed a better anti-rheumatic activity in rats among other fractions.Keywords: Muntingia calabura L. leaves, Ethanol extract, N-hexane fraction, Ethyl acetate fraction, Antirheumatic.

scholarly journals ANTIOXIDANT ACTIVITY OF TARENNA POLYCARPA (MIQ.) KOORD. LEAF

2018 ◽  
Vol 11 (4) ◽  
pp. 224
Author(s):  
Rika Puspita Sari ◽  
Marline Nainggolan ◽  
Rosidah Rosidah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Hexane Fraction ◽  
Dpph Assay

 Objective: The objective of this study is to evaluate the antioxidant activity of Tarenna polycarpa (Miq.) koord. Ex Valenton. Leaf extract and fractions.Methods: Antioxidant activity was examined by DPPH method.Results: Ethanol extract, n-hexane fraction, and ethyl acetate fraction with DPPH assay measured as half maximal inhibitory concentration were 55.21, 109.73, and 42.04 μg/mL, respectively.Conclusions: The results reveal that T. polycarpa extract and fractions have strong antioxidant potential. Our further study is to isolate compounds responsible for antioxidant components.

scholarly journals SKRINING FITOKIMIA DAN UJI TOKSISITAS EKSTRAK DAUN PECUT KUDA (Stachytarpheta jamaicensis L. Vahl) TERHADAP LARVA UDANG Artemia salina Leach

2006 ◽  
Vol 12 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Lany Indrayani ◽  
Hartati Soetjipto ◽  
Lydia Sihasale
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethyl Acetate Fraction ◽  
Artemia Salina ◽  
Hexane Fraction ◽  
Chloroform Fraction ◽  
Phytochemical Screening ◽  
Chemical Substances ◽  
Toxicity Effect

Phytochemical screening and Brine Shrimp lethality test of pecut kuda leaf extract had been done against Artemia salina Leach. The aims of this study is to screen potentially bioactive extract of pecut kuda leaf as an effort ti find out the chemical substances responsible for the toxic effect. The Pecut kuda leaves (Stachytarpheta jamaicensis, L. Vahl) was extracted by methanol 80 percent, then partitioned successively with hexane, chloroform, and ethyl acetate. Phytochemical screening was done according to Ciulei method (1984). The toxicity effect was tested against A. salina, L (Brine Shrimp Test). The result of phytochemical screening known that compounds in the extract of pecut kuda leaves (S. jamaicensis, L. Vahl), hexane fraction contained of sterol and triterpenes; where the chloroform fraction contained of saponin, sterol, and triterpenes; further more the ethyl acetate fraction contained flavonoid, tannin, saponin, sterol, dan triterpenes. The result of the study shows that several fractions of extract of pecut kuda leaves (S. jamaicensis) hexane fraction, chloroform, and ethyl acetate was toxic because it was able to kill more than 50 percent larva of A. salina, L at the concentration less than 1000 ppm. The LC50 of all fractions of pecut kuda leaves extract (S. jamaicensis, L. Vahl) were shown from the most toxic to the lowest were as followed: LC50 of hexane fraction = 98.33 ppm, of chloroform raction LC50 = 204.17 ppm, and of ethyl acetate fraction LC50 = 249.80 ppm. Finally results of the TLC profile showed that all fraction (hexane, chloroform, and ethyl acetate) indicated that the pecut kuda leaves extract (S. jamaicensis) contained terpenoid compound.

scholarly journals Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua

2020 ◽  
Vol 12 (2) ◽  
pp. 93-101
Author(s):  
Verena Agustini ◽  
Eva S. Simaremare ◽  
Elsye Gunawan ◽  
Jane Awom ◽  
Susan Wopi
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Cytotoxic Potential ◽  
Stem Extract ◽  
Ethanol Fraction

The aims of the study are to evaluate bioactive compounds, antibacterial and cytotoxic potential of D. lasianthera. This orchid grows well all over New Guinea Island as an ornamental plant because of their beautiful flowers. Orchids also known rich of its phytochemical compounds which already used as a traditional medicine in many countries around the world. However, research in pharmacological fields is still limited. In this study, leaves and stem of D. lasianthera were powdered and extracted with ethanol followed by fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening and determined antibacterial activity against Escherichia coli and Staphylococcus aureus using Disc Diffusion Method. Brine Shrimp Lethality Test (BSLT) was used to observe cytotoxic potential of leaves and stem extract and fraction at 10, 50, 100, 250, 500, 750, and 1000 ppm. The results showed that the leaves and stem extract contained tannin and alkaloids, separately. The ethanol extract of D. lasianthera showed 7.35 mm (leaves) and 7.43 mm (stem) inhibition zone against S. aureus Furthermore, the maximum inhibition zone of ethanol fraction of leaves and n-hexane fraction of stem were 8.42 mm and 8.10 mm separately. The LC50 of stem extract and fractions in these study were 699.3 ppm (ethanol extract), 602.1 ppm (ethyl acetate), 329.6 ppm (n-hexane fraction) and 676 ppm (ethanol fraction), whereas for leaves, only ethyl acetate fraction has toxict activity with an average LC50 833.2 ppm. Key words: D. lasianthera; phytochemical screening; antibacterial; cytotoxicity; Papua. 

scholarly journals In Vitro Test of Antibacterial Ethanol Extract, n-Hexane Fraction and Ethyl acetate Fraction of Sungkai Leaf (Peronema cenescens) Against Salmonella typhi

1970 ◽  
Vol 8 (3) ◽  
pp. 59-61
Author(s):  
Sitepu Nadroh Br.
Keyword(s):  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Salmonella Typhi ◽  
Hexane Fraction ◽  
In Vitro Test ◽  
Leaf Powder ◽  
Vitro Test

Object: This study aims to look at the class of compounds and the comparison of the antibacterial activity of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Methods: Study included phytochemical screening and in vitro antibacterial testing of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Results: obtained groups of chemical compounds alkaloids, flavonoids, glycosides, anthraquinones, tannins and triterpenoids/steroids on Sungkai leaf powder. Ethanol extract of Sungkai leaves obtained resistance at a concentration of 20% by 12.7 mm, and inhibition of the ethyl acetate fraction at a concentration of 20% of 14.8 mm. Conclusion: Ethyl acetate fraction of Sungkai leaves have antibacterial properties against S. typhi which is greater than ethanol extract and hexane fraction of leaf heal.  

Screening of anti-HIV activities in ethanol extract and fractions from Ficus fistulosa leaves

2021 ◽  
Vol 32 (4) ◽  
pp. 737-742
Author(s):  
Siti Qamariyah Khairunisa ◽  
Dwi Wahyu Indriati ◽  
Lidya Tumewu ◽  
Aty Widyawaruyanti ◽  
Nasronudin Nasronudin
Keyword(s):  
Antiviral Activity ◽  
Ethyl Acetate ◽  
Opportunistic Infections ◽  
Antiviral Drug ◽  
Ethanol Extract ◽  
Syncytium Formation ◽  
Ethyl Acetate Fraction ◽  
Chloroform Fraction ◽  
High Level ◽  
Anti Hiv

Abstract Objectives Human immunodeficiency virus (HIV) infection is considered as a major immunosuppressive disease linked to malignancies and other opportunistic infections. Recently, the high prevalence of HIV drug-resistant strains required a high demand for novel antiviral drug development, especially in herbal medicine approaches. The objective of this study was to evaluate the possibility of Ficus fistulosa leaves can inhibit HIV replication in ethanol extract form as well as its fractions using chloroform, ethyl acetate, and butanol solvents. Methods F. fistulosa leaves were extracted using ethanol as a solvent and further gradually fractionated in chloroform, ethyl acetate, and butanol solvents. The targeted persistently infected virus (MT4/HIV) cell lines were cocultured with ethanol extract and fractions at different time points. The syncytium formation and cytotoxicity assays were performed to evaluate the potential antiviral activity of F. fistulosa leaves. Results One of the four tested extract/fractions showed antiviral activity against HIV. The ethanol extract showed weak inhibition with a high level of toxicity (IC50 = 8.96 μg/mL, CC50 ≥50 μg/mL, and SI = 5.58). Meanwhile, chloroform fraction effectively inhibited the MT4/HIV cell proliferation while keeping the toxicity to a minimal level (IC50 = 3.27 μg/mL, CC50 = 29.30 μg/mL, and SI = 8.96). In contrast of ethyl acetate fraction and butanol fraction showed no anti HIV activity with a high level of toxicity (CC50 ≥50 μg/mL) and low SI value (>2.17 μg/mL and >0.97 μg/mL). Conclusions Chloroform fraction of F. fistulosa leaves showed effectively as anti-viral activity against MT4/HIV cells.

Sign in / Sign up

Export Citation Format

Share Document