scholarly journals THE ANTIRHEUMATIC ACTIVITY OF MUNTINGIA CALABURA L. LEAVES ETHANOL EXTRACT AND ITS FRACTION

2016 ◽  
Vol 10 (1) ◽  
pp. 84
Author(s):  
Jamilah Sarimanah ◽  
Ketut Adnyana I ◽  
Elin Yulinah Sukandar ◽  
Neng Fisheri Kurniati
Keyword(s):  
Ethyl Acetate ◽  
Chronic Inflammation ◽  
Ethanol Extract ◽  
Inflammatory Cells ◽  
Ethyl Acetate Fraction ◽  
Cartilage Destruction ◽  
Hexane Fraction ◽  
Intraplantar Injection ◽  
The Right ◽  
Muntingia Calabura

ABSTRACTObjective: Rheumatoid arthritis is chronic inflammation disorder that can be treated with anti-inflammatory drug such as methylprednisolone.Several medicinal plants shave the potential to be used in arthritis therapy, one of which is Muntingia calabura L. The objective of this research wasto study the antirheumatic activity of M. calabura leaves ethanol extract and its n-hexane, ethyl acetate, and water fractions in a rheumatoid arthriticrat model.Methods: Rheumatoid was induced in rats by intraplantar injection of Complete Freund’s Adjuvant (CFA) into the right foot paw of Wistar rats. Thepaw volume was measured by a plethysmometer. The histology of right foot paw was carried out. For chronic CFA inflammation, induced paw edemaassay was used, whereas chronic inflammation was assessed by CFA induced arthritis. The histopathology of synovial joints (paw) was carried out.Result: The results of the study revealed that in CFA-induced arthritic rats, methylprednisolone, M. calabura ethanol extract, and its fractions wereable to reduce inflammation. Histopathology examination also showed a reduction of in cartilage destruction, influx of inflammatory cells, pannusformation, fibrin deposition, and synovitis.Conclusion: M. calabura L. n-hexane fraction showed a better anti-rheumatic activity in rats among other fractions.Keywords: Muntingia calabura L. leaves, Ethanol extract, N-hexane fraction, Ethyl acetate fraction, Antirheumatic.

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

scholarly journals Screening Bioactivity of Kersen Fruits (Muntingia calabura L.) as a Sunscreens Candidate

2021 ◽  
Vol 7 (1) ◽  
pp. 29-38
Author(s):  
Syamsu Nur ◽  
Nursamsiar Nursamsiar ◽  
Muhammad Aswad ◽  
Aprilia Ester Eunike Tumigolung ◽  
Risfah Yulianti ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Active Ingredient ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Content ◽  
In Vitro Method ◽  
Best Value ◽  
Phenolic And Flavonoid ◽  
Muntingia Calabura

Kersen (Muntingia calabura L) fruits have the potential to be used as an active ingredient in sunscreens because of phenolic and flavonoid content that can absorb UV rays. This study aims to determine the percentage of erythema/pigmentation transmission and SPF value as parameters for sunscreen activity.  Kersen fruits were extracted by maceration using 96% ethanol. The ethanol extract of Kersen Fruits was also fractionated to separate the components of the active compounds based on the polarity level using n-hexane, ethyl acetate, and ethanol as solvents. The test was carried out using the in vitro method by measuring the ability of the material to absorb ultraviolet light at a wavelength of 292.5-372.5 nm. This research was conducted at concentrations of 100, 200, 400, 600, and  800 µg/mL for ethanol, lyophilisate, n-hexane, and ethanol fractions, while the ethyl acetate fraction concentrations are 50, 100, 150, 200, 250 µg/mL. The results showed the best value at the ethyl acetate fraction concentration of 250 µg/mL with % Te of 5.28 and % Tp of 28.65 and the SPF value of 16.54. Based on the % Te and Tp, the ethyl acetate fraction exhibited protection against erythema and pigmentation with the category of extra protection and based on the SPF value with the category of ultra protection.

scholarly journals ANTIOXIDANT ACTIVITY OF TARENNA POLYCARPA (MIQ.) KOORD. LEAF

2018 ◽  
Vol 11 (4) ◽  
pp. 224
Author(s):  
Rika Puspita Sari ◽  
Marline Nainggolan ◽  
Rosidah Rosidah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Hexane Fraction ◽  
Dpph Assay

 Objective: The objective of this study is to evaluate the antioxidant activity of Tarenna polycarpa (Miq.) koord. Ex Valenton. Leaf extract and fractions.Methods: Antioxidant activity was examined by DPPH method.Results: Ethanol extract, n-hexane fraction, and ethyl acetate fraction with DPPH assay measured as half maximal inhibitory concentration were 55.21, 109.73, and 42.04 μg/mL, respectively.Conclusions: The results reveal that T. polycarpa extract and fractions have strong antioxidant potential. Our further study is to isolate compounds responsible for antioxidant components.

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

scholarly journals Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua

2020 ◽  
Vol 12 (2) ◽  
pp. 93-101
Author(s):  
Verena Agustini ◽  
Eva S. Simaremare ◽  
Elsye Gunawan ◽  
Jane Awom ◽  
Susan Wopi
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Cytotoxic Potential ◽  
Stem Extract ◽  
Ethanol Fraction

The aims of the study are to evaluate bioactive compounds, antibacterial and cytotoxic potential of D. lasianthera. This orchid grows well all over New Guinea Island as an ornamental plant because of their beautiful flowers. Orchids also known rich of its phytochemical compounds which already used as a traditional medicine in many countries around the world. However, research in pharmacological fields is still limited. In this study, leaves and stem of D. lasianthera were powdered and extracted with ethanol followed by fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening and determined antibacterial activity against Escherichia coli and Staphylococcus aureus using Disc Diffusion Method. Brine Shrimp Lethality Test (BSLT) was used to observe cytotoxic potential of leaves and stem extract and fraction at 10, 50, 100, 250, 500, 750, and 1000 ppm. The results showed that the leaves and stem extract contained tannin and alkaloids, separately. The ethanol extract of D. lasianthera showed 7.35 mm (leaves) and 7.43 mm (stem) inhibition zone against S. aureus Furthermore, the maximum inhibition zone of ethanol fraction of leaves and n-hexane fraction of stem were 8.42 mm and 8.10 mm separately. The LC50 of stem extract and fractions in these study were 699.3 ppm (ethanol extract), 602.1 ppm (ethyl acetate), 329.6 ppm (n-hexane fraction) and 676 ppm (ethanol fraction), whereas for leaves, only ethyl acetate fraction has toxict activity with an average LC50 833.2 ppm. Key words: D. lasianthera; phytochemical screening; antibacterial; cytotoxicity; Papua. 

scholarly journals In Vitro Test of Antibacterial Ethanol Extract, n-Hexane Fraction and Ethyl acetate Fraction of Sungkai Leaf (Peronema cenescens) Against Salmonella typhi

1970 ◽  
Vol 8 (3) ◽  
pp. 59-61
Author(s):  
Sitepu Nadroh Br.
Keyword(s):  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Salmonella Typhi ◽  
Hexane Fraction ◽  
In Vitro Test ◽  
Leaf Powder ◽  
Vitro Test

Object: This study aims to look at the class of compounds and the comparison of the antibacterial activity of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Methods: Study included phytochemical screening and in vitro antibacterial testing of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Results: obtained groups of chemical compounds alkaloids, flavonoids, glycosides, anthraquinones, tannins and triterpenoids/steroids on Sungkai leaf powder. Ethanol extract of Sungkai leaves obtained resistance at a concentration of 20% by 12.7 mm, and inhibition of the ethyl acetate fraction at a concentration of 20% of 14.8 mm. Conclusion: Ethyl acetate fraction of Sungkai leaves have antibacterial properties against S. typhi which is greater than ethanol extract and hexane fraction of leaf heal.  

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

2019 ◽  
pp. 8-14
Author(s):  
Risa Supringrum ◽  
Siti Jubaidah
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Hexane Fraction ◽  
High Dose ◽  
Water Fraction ◽  
Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

scholarly journals Antiproliferation activity of God's crown fruit (Phaleria macrocarpa) extract and fractions against MCM-B2 breast cancer cells

2020 ◽  
Author(s):  
Ratna Puspita ◽  
Hasim ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Water Fraction ◽  
Activity Test ◽  
Phaleria Macrocarpa

Breast cancer is a disease caused by abnormal cell proliferation in the breast. God’s crown fruit (Phaleria macrocarpa) and its seed have potential as an antiproliferation of cancer cells. It contains active compounds such as flavonoids, alkaloids, polyphenols, and tannins. The sample of God’s crown fruit was obtained by extraction and fractionation using the maceration method. Cytotoxicity of extracts and fractions was determined using the Brine Shrimp Lethality Test method. The antiproliferation activity test of God’s crown fruit against MCM-B2 was performed using the hemacytometer method. The God’s crown fruit sample consists of crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. Lethal concentration 50 (LC50) values in crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 13.72, 147.55, 405.81, and 149.55 ppm, respectively. The concentration of the test sample was directly used for the antiproliferation activity test on MCM-B2 cells. God’s crown fruit can act as an antiproliferation of MCM-B2. The smallest concentration of those samples has inhibited MCM-B2 cell proliferation which is 3.5 ppm crude ethanol extract lower than 100 ppm doxorubicin. The maximum percentage of the antiproliferation activity of crude ethanol extract (56 ppm) was able to inhibit MCM-B2 cell proliferation by 58.28% while doxorubicin (100 ppm) by 31.2%. This is due to the fact that crude ethanol extract has a lot of complex polar phytochemical content. The crude ethanol extract compounds inhibit MCM-B2 cell proliferation synergistically.

scholarly journals Aktivitas sitotoksik dan antiangiogenesis umbi mentimun papasan (Coccinia grandis L.Voight) terhadap sel kanker hela yang diinduksi protein bFGF

2020 ◽  
Vol 9 (1) ◽  
pp. 51
Author(s):  
Julia Megawati Djamal ◽  
Jason Merari P ◽  
Rizal Maarif Rukmana
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Chorioallantoic Membrane ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Assay Method ◽  
Water Fraction ◽  
Coccinia Grandis

Background: Cancer is a leading cause of death in Indonesia and in the world. Cervical cancer is one of the most common cancers in women. One of the plants that has the potential to be developed as an alternative treatment for cancer is the papasan cucumber tuber (Coccinia grandis (L) Voight). This study aims to determine the activity of cytotoxic effects on Hela cancer cells and antiangiogenesis activity against Chorioallantoic membrane. Method: In this study, papasan cucumber tubers were extracted with 96% ethanol solvent. The ethanol extract was then continued with fractionation using the liquid-liquid partition method, followed by thin-layer chromatography used to determine of compounds contained in the papasan cucumber tubers. Cytotoxic activity tests were carried out using the MTT assay method and antiangiogenesis tests were carried out using CAM methods of chicken embryos induced with bFGF protein. Results: The results showed that the extract, n-hexane fraction, ethyl acetate fraction and water fraction had IC50 values 986,604 µg/ml, 208,776 µg/ml, 187,824 µg/ml and 553,393 µg/ml. The IC50 value of the n-hexane fraction was then continued with antiangiogenesis testing. The KLT analysis showed that the n-hexane fraction contained alkaloids, flavonoids, tannins, saponins, and terpenes. Antiangiogenesis test result show that the inhibitory at concentration of 104 µg / ml was 17,56%, at a concentration of 208 µg / ml 43,23% and at a concentration of 418 µg / ml 56,78%. Conclusion: n-hexane fraction and ethyl acetate fraction have moderate cytotoxic activity against Hela cancer cells and n-hexane fraction has antiangiogenesis activity. Key words: Coccnia grandis L. Voight, Cytotoxic, Antiangiogenesis, Hela, Chorioallantoic Membrane

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