scholarly journals Kerevit (Astacus leptodactylus) Yemine Katılan Selenyumun Paraoksonaz ve Arilesteraz Enzim Aktivitelerine Etkisi

2019 ◽  
Vol 7 (2) ◽  
pp. 240
Author(s):  
Serpil Mişe Yonar ◽  
Muzaffer Harlıoğlu
Keyword(s):  
Total Energy ◽  
Enzyme Activities ◽  
Crude Protein ◽  
Energy Levels ◽  
Egg Laying ◽  
Control Group ◽  
Astacus Leptodactylus ◽  
Tissue Samples ◽  
Positive Effects ◽  
Egg Carrying

In this study, the effects of selenium added to the diets of Astacus leptodactylus at different ratios on paraoxonase (PON) and arylesterase enzyme activities (ARE) in the hepatopancreas and gonad tissues were investigated. In this study, control (K), trial 1 (D1), trial 2 (D2) and trial 3 (D3) were prepared at the selenium levels of 0,3, 0,6, 0,9 and 1,2 mg/kg, respectively. The crude protein and total energy levels of experimental diets were equalized. 12 ponds in 2 × 2 × 1 m dimensions were used. 75 female and 25 male crayfish were stocked in each pond (totally 1200 crayfish). This study was carried out in triplicate. The crayfish were fed twice a day during 9 months. PON and ARE enzyme activities were investigated in the tissue samples taken monthly from the crayfish. During the trial, significant differences were observed in the PON and ARE enzyme activities in the hepatopancreas and gonad tissues. The PON and ARE enzyme activities increased statistically significant in the crayfish tissues during the breeding season and incubation period. This increase was found to be statistically different in the D1, D2 and D3 groups compared to the control group. In conclusion, selenium had positive effects on PON and ARE enzyme activities of A.leptodactylus during its mating, pleopodal egg laying and pleopodal egg carrying periods.

scholarly journals Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 287-292 ◽  
Author(s):  
AS Durmus ◽  
M. Yaman ◽  
HN Can
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Albino Rats ◽  
Burn Wound ◽  
Tissue Samples ◽  
Burned Areas ◽  
Positive Effects ◽  
Burn Wound Healing

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7<sup>th</sup> and 14<sup>th</sup> days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P &lt; 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P &lt; 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model. &nbsp;

scholarly journals Effects of fructans-containing yacon (Smallanthus sonchifoliusPoepp & Endl.) flour on caecum mucosal morphometry, calcium and magnesium balance, and bone calcium retention in growing rats

2007 ◽  
Vol 97 (4) ◽  
pp. 776-785 ◽  
Author(s):  
Alexandre R. Lobo ◽  
Célia Colli ◽  
Eliana P. Alvares ◽  
Tullia M. C. C. Filisetti
Keyword(s):  
Bone Mass ◽  
Bone Mineral ◽  
Peak Load ◽  
Biomechanical Testing ◽  
Biomechanical Properties ◽  
Control Group ◽  
Mineral Density ◽  
Tissue Samples ◽  
Mineral Absorption ◽  
Positive Effects

Yacon roots have been considered a functional food due to the high levels of fructans they contains. In the present study, Ca and Mg balance, bone mass and strength, and caecum mucosal morphometry were evaluated. Growing male Wistar rats (n24) were fedad libitumcontrol diets or diets supplemented with yacon flour (5 or 7·5 % fructooligosaccharides) for 27 d. Mineral balance was evaluated in three periods of 5 d (starting on the 4th, 10th and 16th days). After the rats were killled, the bones were removed and bone mineral density was measured. Ca analyses were performed on left femurs and tibias and biomechanical testing on right femurs. The caecum was removed and tissue samples were collected for histological analysis. Caecal histology changed noticeably in rats fed yacon flour: there was an increase in the depth and number of total and bifurcated crypts as well. Yacon flour consumption significantly (P < 0·05) resulted in a positive Ca and Mg balance, leading to higher values of bone mineral retention and biomechanical properties (peak load and stiffness) when compared to the control group. The positive effects on mineral intestinal absorption, bone mass and biomechanical properties showed an important role of yacon roots in the maintenance of healthy bones. The increased number of bifurcating crypts might be related to the higher mineral absorption caused by the enlargement of the absorbing surface in the large intestine of the animals.

scholarly journals Effects of clinoptilolite addition to colostrum on the concentration of serum proteins, minerals, enzyme activities in neonatal calves

2014 ◽  
Vol 10 (2) ◽  
pp. 2290-2296 ◽  
Author(s):  
Simona Marc Zarcula ◽  
Călin Mircu ◽  
Gheorghe Bonc ◽  
Danijela Kirovski ◽  
Gabriel Otavă ◽  
...  
Keyword(s):  
Enzyme Activities ◽  
Serum Iron ◽  
Serum Proteins ◽  
Jugular Vein ◽  
Control Group ◽  
Positive Effects ◽  
Calcium Phosphorus ◽  
Newborn Calves ◽  
Neonatal Calves ◽  
Experimental Group

The objective of our research was to determine the effects of colostrum supplementation with clinoptilolite on concentrations of serum proteins, minerals and enzyme activities in newborn calves. Twenty newborn calves that were divided into control group (n=10) which received colostrum and experimental group (n=10) that received colostrum with 0.5% clinoptilolite added in the first three colostrum meals were studied. Blood samples were collected from jugular vein in vacutainer tubes from all calves prior to colostrum intake and after that, at 24 and 48 h after birth. Samples were analyzed for total protein, albumin, α1-globulin, α2-globulin, ß-globulin, γ-globulin, calcium, phosphorus, magnesium, iron, gamma glutamyltransferase, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase. Clinoptilolite supplementation had significant effect (p<0.0006) on serum iron concentrations in experimental (27.64±3.78 µmol/l) vs control group (8.93±1.26 µmol/l) after 48h. GGT values were also significantly higher (p<0.04) in E (163.60±26.67 U/l) than in C group (84.01±19.77 U/l) at 48h after parturition. Other parameters analyzed were unaffected by clinoptilolite treatment. Obtained data revealed that colostrum supplemented with clinoptilolite had positive effects on some mineral parameters.

scholarly journals Effect of Lactobacillus plantarum LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

2016 ◽  
Vol 66 (3) ◽  
pp. 294-303 ◽  
Author(s):  
Emília Hijová ◽  
Jozef Kuzma ◽  
Ladislav Strojný ◽  
Alojz Bomba ◽  
Izabela Bertková ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Enzyme Activities ◽  
Probiotic Strain ◽  
Coliform Bacteria ◽  
Control Group ◽  
Sprague Dawley ◽  
Positive Effects ◽  
Glucuronidase Activity ◽  
Bacterial Enzyme ◽  
Prevention Of Cancer

Abstract The effect of the probiotic strain Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities – β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in the prevention of cancer, atherosclerosis and dysbiosis was investigated. Male Sprague-Dawley rats were randomly divided into 12 experimental groups: C (control group), AT (atherosclerotic group), CC (carcinogenic group), and then each group in combination with antibiotics and probiotics individually and each group in double combination on antibiotic and probiotic. In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in the atherosclerotic group significantly increased the activity of β-glucuronidase (p<0.001) and β-glucosidase (p<0.01). Azoxymethane application in the carcinogenic group significantly increased β-glucuronidase (p<0.01), but reduced β-glucosidase (p<0.01). Daily application of probiotics individually and in double combination with antibiotics increased the activity of β-galactosidase, and β-glucosidase, and positively decreased the level of β-glucuronidase. In the control antibiotic group β-glucuronidase was significantly increased (p<0.05), and β-glucosidase decreased (p<0.01) which can be caused by a change of microflora in favor of coliform bacteria. These finding indicate the positive effects of probiotic Lactobacillus plantarum LS/07 which allows its use in disease prevention in human and veterinary medicine.

scholarly journals Influence of dietary supplementation of autolyzed whole yeast and yeast cell wall products on broiler chickens

2020 ◽  
Vol 33 (4) ◽  
pp. 579-587 ◽  
Author(s):  
Emmanuel Uchenna Ahiwe ◽  
Medani Eldow Abdallh ◽  
Edwin Peter Chang’a ◽  
Apeh Akwu Omede ◽  
Mohammed Al-Qahtani ◽  
...  
Keyword(s):  
Yeast Cell ◽  
Enzyme Activities ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Pancreatic Enzyme ◽  
Protein Digestibility ◽  
Nutrient Digestibility ◽  
Control Group ◽  
Meat Yield ◽  
Positive Effects

Objective: This study evaluated the effect of yeast products on growth performance, visceral organ weights, endogenous enzyme activities, ileal nutrient digestibility and meat yield of broiler chickens fed diets containing autolyzed whole yeast (WY) and yeast cell walls (YCW) at varying levels of inclusion.Methods: Nine dietary treatments consisting of WY or YCW included at 0.5, 1.0, 1.5, or 2.0 g/kg diet and a control diet without yeast supplementation was used in the experiment. Each of the nine treatments was replicated six times with nine birds per replicate. Birds were housed in cages, in climate-controlled rooms and fed starter, grower and finisher diets.Results: There was an improvement (p<0.05) in body weight gain and feed conversion ratio on d 10, 24, and 35 for birds fed 1.0 to 2.0 g/kg WY or YCW diet. Small intestine weight was heavier on d 10 and 24 for birds on higher levels of WY and YCW compared to the control group. On d 10 and 24, there was a significant increase (p<0.05) in tissue protein content and pancreatic enzyme activities (trypsin and chymotrypsin) of birds on 1.5 to 2.0 g/kg WY and YCW diets compared to the control group. Compared to the control group, birds on WY (2.0 g/kg diet) and YCW (at 1.5 and 2.0 g/kg diet) had better (p<0.05) protein digestibility on d 24. On d 35, there was significant improvement (p<0.05) in percentage of carcass, absolute and relative breast weight for broiler chickens fed WY and YCW mostly at 2 g/kg diet compared to birds on the control diet.Conclusion: Supplementation of diets with autolyzed WY and YCW products especially at 1.5 to 2.0 g/kg diet improved broiler chicken performance and meat yield through their positive effects on ileal protein digestibility and pancreatic enzyme activities.

scholarly journals Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions

2020 ◽  
Vol 45 (4) ◽  
pp. 405-414
Author(s):  
Mehmet Kabalci ◽  
Mustafa Sahin ◽  
Zeynep Pekcan ◽  
Mehmet Zengin ◽  
Mehmet Tolga Dogru ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Postoperative Adhesion ◽  
Control Group ◽  
Albino Rats ◽  
Tissue Samples ◽  
Positive Effects ◽  
Membrane Transfer ◽  
Amnion Membrane ◽  
Wistar Albino Rats ◽  
Pericardial Adhesions

AbstractBackgroundTo investigate and compare the antiadhesive/antifibrotic effects of mesenchymal stem cells (MSC) and amnion membrane transfer (AMT) in a rat model.Material and methodsThree experimental and sham groups were formed using 30 Wistar-Albino rats. AMT and MSC were applied to the related groups. The control group was not treated. After 12 weeks follow-up, intracardiac blood and cardiac-pericardiac tissue samples were taken. The severity of adhesions and fibrosis were scored macroscopically and microscopically with Hematoxylin/Eosin and Masson’s trichrome staining. TNF-α, TGF-β, IL-1, PDGF, FGF, VEGF and Caspase-3 levels were measured with the ELISA method.ResultsSevere adhesions were observed in the AMT and control groups, but no adhesion was present in the MSC group. Pericardial thickness, increased vascularity, fibrosis, and collagen accumulation were similar between control and AMT groups, but were less in Sham and MSC groups. Between MSC and AMT groups, only Caspase-3 level was different, which is an apoptosis marker.ConclusionThe positive effects of MSC on adhesion, which we achieved in our study, suggest that it may prevent adhesion. AMT did not provide a positive effect. The correlation of Caspase-3 with postoperative adhesion/fibrosis should be examined in more detail.

The effect of alternate-day caloric restriction on the metabolic consequences of 8 days of bed rest in healthy lean men: a randomized trial

2017 ◽  
Vol 122 (2) ◽  
pp. 230-241 ◽  
Author(s):  
Nina Majlund Harder-Lauridsen ◽  
Signe Tellerup Nielsen ◽  
Sebastian Porsdam Mann ◽  
Mark Preben Lyngbæk ◽  
Fabiana Braga Benatti ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Energy Balance ◽  
Total Energy ◽  
Caloric Restriction ◽  
Visceral Fat ◽  
Bed Rest ◽  
Energy Requirements ◽  
Control Group ◽  
Positive Effects ◽  
The Metabolic Syndrome

Physical activity and alternate-day fasting/caloric restriction may both ameliorate aspects of the metabolic syndrome, such as insulin resistance, visceral fat mass accumulation, and cognitive impairment by overlapping mechanisms. The purpose of this study was to test the hypothesis that alternate-day caloric restriction (ADCR) with overall energy balance would reduce insulin resistance and accumulation of visceral fat, in addition to improving cognitive functions, after 8 consecutive days in bed. Healthy, lean men ( n = 20) were randomized to 1) 8 days of bed rest with three daily isoenergetic meals (control group, n = 10); and 2) 8 days of bed rest with 25% of total energy requirements every other day and 175% of total energy requirements every other day (ADCR group). Oral glucose tolerance testing, dual-energy X-ray absorptiometry (DXA) scans, magnetic resonance imaging of the abdomen and brain, V̇o2max, and tests for cognitive function were performed before and after bed rest. In addition, daily fasting blood samples and 24-h glucose profiles by continuous glucose monitoring system were assessed during the 8 days of bed rest period. Bed rest induced insulin resistance, visceral fat accumulation, and worsening of mood. No positive effects emerged from ADCR on these negative health outcomes. Compared with the control group, ADCR was associated with improved and steadier glycemic control on fasting days and higher glycemic fluctuation and indexes of insulin resistance on overeating days. In contrast to our hypothesis, the metabolic impairment induced by 8 days of bed rest was not counteracted by ADCR with overall energy balance. NEW & NOTEWORTHY Alternate-day caloric restriction without overall energy reduction does not ameliorate the metabolic impairment induced in lean men by 8 days of bed rest.

Effects of a 6-Week Straw Phonation in Water Exercise Program on the Aging Voice

2020 ◽  
Vol 63 (4) ◽  
pp. 1018-1032
Author(s):  
Chia-Hsin Wu ◽  
Roger W. Chan
Keyword(s):  
Acoustic Analysis ◽  
Vocal Tract ◽  
Exercise Program ◽  
Analysis Of Covariance ◽  
Elderly Subjects ◽  
Control Group ◽  
Perceptual Evaluation ◽  
Positive Effects ◽  
Aging Voice ◽  
Before And After

Purpose Semi-occluded vocal tract (SOVT) exercises with tubes or straws have been widely used for a variety of voice disorders. Yet, the effects of longer periods of SOVT exercises (lasting for weeks) on the aging voice are not well understood. This study investigated the effects of a 6-week straw phonation in water (SPW) exercise program. Method Thirty-seven elderly subjects with self-perceived voice problems were assigned into two groups: (a) SPW exercises with six weekly sessions and home practice (experimental group) and (b) vocal hygiene education (control group). Before and after intervention (2 weeks after the completion of the exercise program), acoustic analysis, auditory–perceptual evaluation, and self-assessment of vocal impairment were conducted. Results Analysis of covariance revealed significant differences between the two groups in smoothed cepstral peak prominence measures, harmonics-to-noise ratio, the auditory–perceptual parameter of breathiness, and Voice Handicap Index-10 scores postintervention. No significant differences between the two groups were found for other measures. Conclusions Our results supported the positive effects of SOVT exercises for the aging voice, with a 6-week SPW exercise program being a clinical option. Future studies should involve long-term follow-up and additional outcome measures to better understand the efficacy of SOVT exercises, particularly SPW exercises, for the aging voice.

Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

2006 ◽  
Vol 76 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yukari Egashira ◽  
Shin Nagaki ◽  
Hiroo Sanada
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Enzyme Activities ◽  
Treated Group ◽  
Control Group ◽  
Puromycin Aminonucleoside ◽  
Low Level ◽  
Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

Experimental increase in female mass during the fertile phase leads to enhanced extra-pair paternity in pied flycatchers Ficedula hypoleuca

2019 ◽  
Author(s):  
Mireia Plaza ◽  
Alejandro Cantarero ◽  
Juan Moreno
Keyword(s):  
Negative Relationship ◽  
Ficedula Hypoleuca ◽  
Egg Laying ◽  
Female Dominance ◽  
Laying Date ◽  
Control Group ◽  
Wing Loading ◽  
Potential Fecundity ◽  
Fertile Phase ◽  
Extra Pair Paternity

Female mass in most altricial birds reaches its maximum during breeding at egg-laying, which coincides temporally with the fertile phase when extra-pair paternity (EPP) is determined. Higher mass at laying may have two different effects on EPP intensity. On the one hand, it would lead to increased wing loading (body mass/wing area), which may impair flight efficiency and thereby reduce female’s capacity to resist unwanted extra-pair male approaches (sexual conflict hypothesis). On the other hand, it would enhance female condition, favouring her capacity to evade mate-guarding and to search for extra-pair mates (female choice hypothesis). In both cases, higher female mass at laying may lead to enhanced EPP. To test this prediction, we reduced nest building effort by adding a completely constructed nest in an experimental group of female pied flycatchers (Ficedula hypoleuca). Our treatment caused an increase in mass and thereby wing loading and this was translated into a significantly higher EPP in the manipulated group compared with the control group as expected. There was also a significant negative relationship between EPP and laying date and the extent of the white wing patch, an index of female dominance. More body reserves at laying mean not only a higher potential fecundity but a higher level of EPP as well. This interaction had not previously received due attention but should be considered in future studies of avian breeding strategies.

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