scholarly journals Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 287-292 ◽  
Author(s):  
AS Durmus ◽  
M. Yaman ◽  
HN Can
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Albino Rats ◽  
Burn Wound ◽  
Tissue Samples ◽  
Burned Areas ◽  
Positive Effects ◽  
Burn Wound Healing

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7<sup>th</sup> and 14<sup>th</sup> days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P &lt; 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P &lt; 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model. &nbsp;

scholarly journals Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats

2010 ◽  
Vol 55 (No. 12) ◽  
pp. 619-624 ◽  
Author(s):  
I. Yaman ◽  
AS Durmus ◽  
S. Ceribasi ◽  
M. Yaman
Keyword(s):  
Wound Healing ◽  
Nigella Sativa ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Albino Rats ◽  
Burn Wound ◽  
Tissue Samples ◽  
Burned Areas ◽  
Burn Wound Healing ◽  
Cold Cream

This experiment was conducted in order to compare the effects of Nigella sativa (NS) and silver sulfadiazine (SSD) cream on healing of burn wounds in rats. Fifty four adult, male Wistar-albino rats were divided into three groups of equal numbers. A burn was generated on the backs of all the rats. The burned areas in the first, second and third groups were covered with daily cold cream (control), SSD cream and NS cream (50% NS oil + 50% cold cream), respectively. Four, nine, and 14 days later, the rats were sacrificed and the burned skin tissue samples were collected for histopathological examinations. Histopatological evaluations on the 4th, 9th and 14th days showed burn healing to be better in the NS and SSD groups with respect to the control group. Wound healing was significantly different among the groups at 4th, 9th and 14th days (P &lt; 0.001). In conclusion, application of NS and SSD cream are effective in healing burn related skin wounds in the rat model.

scholarly journals Effects of montelukast on burn wound healing in a rat model

2010 ◽  
Vol 33 (6) ◽  
pp. 413 ◽  
Author(s):  
Muhammet G Turtay ◽  
Cemal Firat ◽  
Emine Samdanci ◽  
Hakan Oguzturk ◽  
Serkan Erbatur ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Macrophage Infiltration ◽  
Control Group ◽  
Fibroblast Proliferation ◽  
Burn Wound ◽  
Leukotriene D4 ◽  
Topical Antibiotics ◽  
Burn Wound Healing ◽  
Local Burn

Purpose: Montelukast, a selective cysteinyl leukotriene D4-receptor antagonist, is used in the treatment of asthma. In a rat model, our aim was to investigate the effects of montelukast, alone or in combination with topical antibiotics, on local burn wound healing. Methods: Rats were randomly allocated to four groups after local burn development: Group 1; rats were left to secondary healing without treatment, Group 2; a dose of 10 mg/kg montelukast was given by gastric gavage once a day for 10 days, Group 3; rats were treated with topical pomade (bacitracin neomycin sulphate), and Group 4; rats were treat with a combination of topical antibiotic and montelukast (10 mg/kg were given by gastric gavage once a day for 10 days). Skin biopsies were taken on days 3, 10, 14, and 20 relative to burn induction. Results: Reepithelialization in the pomade and montelukast+pomade groups on the 10th day was significantly greater, in comparison with control and montelukast groups (p < 0.05). For the montelukast group, edema (on the 14th day) and angiogenesis, fibroblast proliferation, edema and macrophage infiltration (on the 20th day) were statistically improved in comparison with the control group (p < 0.05). For the montelukast+pomade group, angiogenesis, fibroblast proliferation and macrophage infiltration (on the 10th day), and angiogenesis, fibroblast proliferation, edema and macrophage infiltration (on the 14th and 20th days) were statistically improved in comparison with the control group (p < 0.05). Conclusion: In conclusion, montelukast was effective on burn wound healing. Moreover, the effect was amplified when combined with topical antibiotics applied in the early stage of burn wound healing.

scholarly journals Comparison Profile of Nutrition Status in Stage V Chronic Kidney Disease Patients Hemodialyzing Using New and Reuse Dialyzer

2019 ◽  
Vol 5 (2) ◽  
pp. 17
Author(s):  
Yuli Hermansyah ◽  
Ika Aulia Kurniasari ◽  
Heni Fatmawati
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Antioxidant Properties ◽  
Negative Control ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Degree Burn ◽  
Significant Difference

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

scholarly journals Topical treatment of standardised burns with herbal remedies in model rats

2005 ◽  
Vol 5 (4) ◽  
pp. 50-57 ◽  
Author(s):  
Fahir Bečić ◽  
Nedžad Mulabegović ◽  
Zakira Mornjaković ◽  
Elvedina Kapić ◽  
Senad Prašović ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Herbal Remedies ◽  
Control Group ◽  
Therapeutic Effects ◽  
Skin Area ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

The Safety and Efficacy of Mupirocin Topical Spray for Burn Wound Healing in A Rat Model

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Sritharadol Rutthapol ◽  
Chunhachaichana Charisopon ◽  
Kumlungmak Sukanjana ◽  
Buatong Wilaiporn ◽  
Dechraksa Janwit ◽  
...  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Immunohistochemical Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Burn Wound ◽  
Safety And Efficacy ◽  
Burn Wound Healing ◽  
Tgf Β1

ABSTRACT This study evaluated the effect of mupirocin topical spray on burn wound healing in a rat model. Fifteen male Sprague Dawley rats were used to create full-thickness burns on the rat dorsum using a cylindrical stainless steel rod. The rats were topically treated with normal saline solution (NSS), mupirocin spray, ointment, and solution. The wound size and morphological evaluation were investigated by photographs and clinical criterions for wound healing. The histology was observed by hematoxylin and eosin (HandE) staining assay. The immunohistochemical study was evaluated by detection of transforming growth factor-beta 1 (TGF-β1), and the ratio of matrix metalloproteinase-9 to the tissue inhibitor of matrix metalloproteinase-1 (MMP-9/TIMP-1) was quantified using the enzyme-linked immunosorbent assay (ELISA) assay. A complete healing was observed at 28 days in all treatments. Mupirocin formulations accelerated the wound healing faster than NSS in size. However, the clinical criteria indicated a desirable skin appearance in the mupirocin spray and ointment treated groups. The histological evaluations showed no differences between the treatments while the immunohistochemical study revealed that all treatments reduced the level of TGF-β1 over time, particularly on day 28 in the mupirocin spray and ointment treated groups. The MMP-9/TIMP-1 ratio was significantly lower in the mupirocin spray and ointment treated groups than in the NSS and mupirocin solution groups. This study shows the safety and efficacy in the use of mupirocin topical spray. The topical mupirocin spray is an alternative suitable for development as a human topical anti-infective and wound protection spray.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

scholarly journals Preparation and evaluation of burns wound healing ointment base of leaves and stem bark of Anthocleista djalonensis (L) extract using animal model.

2020 ◽  
Vol 1 (02) ◽  
pp. 25-32
Author(s):  
Chinenye Nnenna Ugwu ◽  
Ezinwanne Nneoma Ezeibe ◽  
Chinekwu Sherridan Nwobi ◽  
Chinelo Charity Eze ◽  
Somtochukwu Amuche Evurani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Bark ◽  
Positive Control ◽  
Silver Sulfadiazine ◽  
Wound Contraction ◽  
Bark Extract ◽  
Control Group ◽  
Burn Wound ◽  
Burn Wounds ◽  
Ointment Base

BACKGROUND: Burn wounds are inescapable life events especially in low income areas. Contamination of the burn wound site results in localized wound infection, hence the need for potent phytochemicals readily available for wound healing. The use of stem bark of Anthocleista djalonensis efficacy for burn wound has not been evaluated to the best of our knowledge. AIM: The study is aimed at evaluating and comparing the wound healing potential of ointment base of leaves and Stem bark of Anthocleista djalonensis on burn wounds created on wistar albino rats. METHODS: The leaves and Stem bark of Anthocleista djalonensis collected were extracted using 95%v/v methanol and phytochemical analysis conducted. Simple ointments of varying concentrations were formulated to screen for wound healing activity using the burn wound model on experimental rats grouped into six (n=4). Group 1 was treated with silver sulfadiazine cream (positive control), group 2 with ointment base (negative control), group 3 with 1% stem bark extract ointment, group 4 with 2% stem bark ointment, group 5 with 1% leave extract ointment, and group 6 with 2% leave extract ointment.  All animals were anesthetized before the creation of burn wounds. Measurement was taken on day zero and the wound was left untreated for 48 hours in order to allow bacterial colonization before daily treatment of the wound for 16 days. RESULTS: The result of the phytochemical screening revealed that both extract of Anthocleista djalonensis contains flavonoids, tannins and saponins. On day 2, 1% stem bark, 2% stem bark and 1% leaf extract had 16%, 15%, and 10% wound contraction respectively which was higher than the 8.5% wound contraction of silver sulfadiazine. Also as the concentration of the extract increased, the wound healing effect also increased as seen by the percentage wound contraction on day 16 for all treatment.  CONCLUSION: The findings of the study have shown that methanolic extracts of stem bark and leaf of Anthocleista djalonensis contained bioactive constituents which have burn wound healing activity. The stem bark extract showed better activity when compared with the leaf extract and also the positive control (silver sulfadiazine).    

scholarly journals The Effect of Educated Platelets on the Healing Process of Burn Wounds in Rats: Educated Platelets in Rat Burn Wound Healing

2021 ◽  
Vol 8 ◽  
Author(s):  
Orkun Ilgen ◽  
Sefa Kurt ◽  
Osman Yilmaz ◽  
Murat Celiloglu
Keyword(s):  
Wound Healing ◽  
Burn Injury ◽  
Healing Process ◽  
Recovery Period ◽  
Control Group ◽  
Study Group ◽  
Second Line ◽  
Burn Wound ◽  
Wound Area ◽  
Burn Wound Healing

Burns are serious life-threatening health problems. This study was performed to determine the effect of educated platelets on burn wound healing process. 28 female wistar albino 200-220 gr rats were randomly divided into four groups. Group A1 rats(n:7) were the first-line burnt group from which blood samples are extracted to develop platelet-rich plasma(PRP) with the educated platelets that have a response to burn injury. Group B1 rats(n:7) were the unburnt group with ordinary platelets. Group A2 rats(n:8) were the second-line burnt group which was given PRP with educated platelets. Group B2 rats(n:6), as control group, were the second-line burnt group which was given PRP with ordinary platelets. Photos of rats' dorsum were taken by digital camera on the first day and 21st day of the study. Wound healing was determined by scar surface area. In the study group (Group A2) mean wound area was  53±37 mm², in the control group (Group B2) mean wound area was 114±55 mm² on the last day of the experiment. The sizes of the wounded areas were significantly lower in the study group compared with the control group (p: 0.039). Educated platelets seem to facilitate the recovery period of burn wound healing in rats.

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