scholarly journals Feasibility study of fluorescence quantitative PCR for the detection of microecological dynamics in fermented maize stover feeds

2021 ◽  
Vol 26 (5) ◽  
pp. 2986-2993
Author(s):  
HUAYOU CHEN ◽  
◽  
CHENXI LU ◽  
TINGTING LI ◽  
LINGYU KANG ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Quantitative Method ◽  
Corn Stalk ◽  
Plate Method ◽  
Dynamic Changes ◽  
The Third ◽  
Fluorescence Quantitative Pcr ◽  
Colony Counting ◽  
Pcr Method ◽  
Number Of Bacteria

During the fermentation of corn stalk bio-feed, the quantity of bacteria in corn stalk bio-feed was counted by fluorescence quantitative PCR and plate colony counting respectively. The comparative analysis of these studies was used to explore the feasibility of fluorescence quantification methods and changes in microbiota during fermentation. The results showed that the standard deviation of fluorescence quantitative method was smaller than that of plate method, but the trend was similar. The biomass of Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae reached their maximum on the third, fifth and fifth day respectively, and then decreased gradually and maintained at a certain level. The experiment showed that the fluorescence quantitative PCR method can accurately quantify the number of bacteria in corn stalk bio-feed, and it is a better method to quantitatively detect the dynamic changes of different kinds of bacteria in corn stalk bio-feed.

scholarly journals Application of quantitative PCR for detection of Mycoplasma suis in blood samples

2014 ◽  
Vol 58 (4) ◽  
pp. 533-539
Author(s):  
Artur Jabłoński ◽  
Dominika Borowska ◽  
Sylwia Zębek ◽  
Andrzej Kowalczyk ◽  
Arkadiusz Dors ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Pcr ◽  
Quantitative Method ◽  
Taqman Probe ◽  
Blood Samples ◽  
Mycoplasma Suis ◽  
Coefficients Of Variation ◽  
Pcr Method ◽  
Detection And Quantification

Abstract The aim of the study was to develop and validate a real-time PCR method, using a TaqMan probe, for quantification of Mycoplasma suis in porcine blood. No PCR signals with closely related non-haemotrophic mycoplasmas were obtained. The detection limit of PCR for plasmid combined with blood DNA was determined to be 103/reaction (5 μL of DNA) (1.2x105 target copies in 1 mL of blood). The linearity of real-time PCR (near 1) indicates its use as a quantitative method. Real-time and quantitative PCR were sensitive and specific for the detection and quantification of M. suis in the blood of animals with acute and chronic form of eperythrozoonosis. Developed quantitative PCR cannot be used to detect carrier animals with a small amount of M. suis in their blood. The validity of real-time PCR used in the studies was confirmed by the low inter- and intra-assay coefficients of variation. This fact confirms the applicability of the assay in other laboratories.

scholarly journals Complete Genome and Plasmid Sequences of Seven Isolates of Salmonella enterica subsp. enterica Harboring the mcr-1 Gene Obtained from Food in China

2019 ◽  
Vol 8 (31) ◽  
Author(s):  
Yujie Hu ◽  
Scott V. Nguyen ◽  
Chang Liu ◽  
Wei Wang ◽  
Yinping Dong ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Salmonella Enterica ◽  
Complete Genome ◽  
Content Type ◽  
Fluorescence Quantitative Pcr ◽  
Complete Genomes ◽  
Pcr Method

Seven Salmonella enterica subsp. enterica isolates were identified as carrying the mcr-1 gene, by using a real-time fluorescence quantitative PCR method, from a total of 2,558 isolates which were cultured from various food origins in China between 2011 and 2016. Few complete genomes of Salmonella strains harboring the mcr-1 gene have been reported to date, so we report here the complete genome and plasmid sequences of all of these isolates to provide useful references for understanding the prevalence of foodborne Salmonella enterica subsp. enterica isolates carrying mcr-1.

scholarly journals Identification of Lactic Acid Bacteria in the corn stalk Fermentation by Fluorescence Quantitative PCR

2021 ◽  
Vol 26 (5) ◽  
pp. 2926-2935
Author(s):  
HUAYOU CHEN ◽  
◽  
KANGTAO CAI ◽  
LINGYU KANG ◽  
TINGTING LI ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Quantitative Pcr ◽  
Bifidobacterium Longum ◽  
Lactobacillus Rhamnosus ◽  
Corn Stalk ◽  
Mixed Fermentation ◽  
Fluorescence Quantitative Pcr ◽  
Fermented Feed ◽  
Mixed Bacteria

Lactic acid bacteria play an important role in the fermentation of biological feed. In this experiment, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus casei and Lactobacillus rhamnosus were used to ferment corn stalk by single and mixed bacteria. The changes of Lactobacillus flora during fermentation were analyzed by fluorescence quantitative PCR. The results showed that the maximum number of lactic acid bacteria appeared on the 7th day of mixed fermentation, and the total bacteria content could be significantly increased by adding five kinds of lactic acid bacteria at the same time, and the number of each bacteria in mixed fermentation was close to that measured by single fermentation, which indicated that these five kinds of lactic acid bacteria could cooperate during fermentation and help to improve the quality of stalk fermented feed.

Establishment and Preliminary Application of Multiplex Fluorescent Quantitative PCR for Simultaneous Detection of BVDV, BRV and BCV

2021 ◽  
Author(s):  
Liyun Chang ◽  
Zhiyong Liu ◽  
Yuelan Zhao ◽  
Yan Li ◽  
Jianhua Qin
Keyword(s):  
Quantitative Pcr ◽  
Quantitative Methods ◽  
Diarrheal Disease ◽  
Quantitative Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
N Gene ◽  
Established Method ◽  
Diarrhea Virus ◽  
Fluorescence Quantitative Pcr

Background: In this study, we aimed to establish a multiplex fluorescence quantitative polymerase chain reaction (PCR) method for the identification and detection of bovine viral diarrhea virus (BVDV), bovine rotavirus (BRV) and bovine coronavirus (BCV). Methods: Based on the highly conserved sequences of BVDV E2 gene, BRV VP6 gene and BCV N gene in GenBank, specific primers were designed to amplify the target gene fragments of each virus and the reaction conditions and system were optimized. Multiple fluorescence quantitative methods were established by fluorescence quantitative PCR. Result: The minimum detection limits of plasmid standards for BVDV, BRV and BCV by multiplex fluorescence quantitative PCR were 1.19×102 copies/μL, 3.89×101 copies/μL and 3.74×101 copies/μL, respectively. The lowest sensitivity of the established method was 100 times higher than that of conventional PCR and had high sensitivity. Furthermore, BVDV, BRV and BCV were amplified specifically, with no cross-reactivity with Escherichia coli (E. coli), Salmonella and infectious bovine rhinotracheitis virus (IBRV). The intra-and inter-group coefficients of variation were less than 1%, showing good assay repeatability. Using the established method and ordinary multiplex PCR to simultaneously detect 150 clinical diarrheal disease material samples, the coincidence rate of samples with mixed infection of the three viruses was 83.3%. The results showed that the multiplex fluorescent quantitative PCR detection method established in this study provides a rapid, sensitive and specific technique for clinical diagnosis and epidemiological monitoring of BVDV, BRV and BCV.

Translator and translation research: general descriptions of concepts

2019 ◽  
Author(s):  
Darian Jancowicz-Pitel
Keyword(s):  
Quantitative Method ◽  
Point Of View ◽  
Translation Process ◽  
Translation Research ◽  
Qualitative And Quantitative ◽  
The Third ◽  
Part Time ◽  
Set Up ◽  
Translation Errors ◽  
Multiple Languages

The presented paper aimed for exploring the translation process, a translator or interpreter needs equipment or tools so that the objectives of a translation can be achieved. If an interpreter needs a pencil, paper, headphones, and a mic, then an interpreter needs even more tools. The tools required include conventional and modern tools. Meanwhile, the approach needed in research on translation is qualitative and quantitative, depending on the research objectives. If you want to find a correlation between a translator's translation experience with the quality or type of translation errors, a quantitative method is needed. Also, this method is very appropriate to be used in research in the scope of teaching translation, for example from the student's point of view, their level of intelligence regarding the quality or translation errors. While the next method is used if the research contains translation errors, procedures, etc., it is more appropriate to use qualitative methods. Seeing this fact, these part-time translators can switch to the third type of translator, namely free translators. This is because there is an awareness that they can live by translation. These translators set up their translation efforts that involve multiple languages.

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