scholarly journals Isolation and Identification of Candida Species and Some Superficial Mycosis Associated with Diaper Dermatitis in Erbil City

2021 ◽  
Vol 11 (1) ◽  
pp. 98-103
Author(s):  
Vian B. Nehmatulla
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Life Time ◽  
Diaper Dermatitis ◽  
Candida Spp ◽  
Isolation And Identification ◽  
High Association ◽  
Superficial Mycosis ◽  
High Prevalence ◽  
Hot Season

Diaper dermatitis (DD) is the most common cutaneous diagnosis in infancy that affects almost every child during the early months of their life time; most cases are associated with the yeast colonization of Candida or DD candidiasis and some dermatophytes. It is an irritating and inflammatory acute dermatitis in the perineal and perianal areas resulting from the occlusion and irritation caused by diapers. The study aimed to assess the association between Candida species and some dermatophytes in infants with napkin dermatitis and determine the prevalence of DD in relation with yeast (Candida) colonization and identify common Candida spp. which is responsible for Candida (fungal) DD among infants. Data from 210 infants aged between (0 and 18) months are collected from January to August 2018 in Raparin Hospital in Erbil City. The cases include 113 (53.80%) male infants and 97 (46.19%) female infants and diagnosed with DD. Results of 210 infant with diaper rash participated in this study. 188 (89.52%) were positive for Candida spp. and Dermatophytes (molds). From the total yeast number, Candida albicans was the most predominant species accounting 128 (84.21%), while 24 (15.78%) were non-albicans and dermatophyte molds among which Malassezias spp that accounted 26 (13.83%) and 10 (5.32%), respectively. The percentage of misdiagnosed cases was 9 (4.28%). Infants at age group 6–12 months showed high frequency with diaper rash 127 (60.47%). The prevalence of DD is higher in urban 120 (57.14%) than rural area 90 (42.85%). Furthermore, high prevalence DD found in hot season than colds was 121 (64.36%) and 67 (35.63%), respectively. In conclusion, I found that there are high association between DD with Candida spp. and dermatophytes and Candida albicans is a common invader of all types of napkin eruption.

scholarly journals Isolation and identification of candida species from various clinical samples in a tertiary care hospital

2017 ◽  
Vol 5 (8) ◽  
pp. 3520 ◽  
Author(s):  
Vignesh Kanna B. ◽  
Amar Kumar G. ◽  
Swapna M. ◽  
Joshy M. Easow
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Secondary Infection ◽  
Tertiary Care ◽  
Vaginal Swab ◽  
Clinical Samples ◽  
Care Hospital ◽  
Inexpensive Method ◽  
Candida Spp ◽  
Isolation And Identification

Background: Candida spp is a member of the normal flora of the skin, mucous membrane and gastrointestinal tract. They are endogenous opportunists which cause secondary infection in individuals with underlying immunocompromised conditions. Candidiasis is a common fungal disease in humans. An increase in the prevalence of non-albicans species has been noted during the last decades because of increasing use of azoles. This study aims to Spectate Candida using chromogenic medium.Methods: A total of 50 Candida isolates from various clinical samples were included in the study. These isolates were subjected to gram's stain, germ tube test and inoculation on commercially available CHROM agar (HiMedia India).Results: In current study majority of isolates were from high vaginal swab (34%) followed by sputum (28%), urine (18%), pus from surgical sites and others constituted to 20%. Candida albicans (51%) was the most common candida species, followed by C. tropicalis (25%), C. krusei (16%), C. glabrata (6%) and C. dubliniensis (1%).Conclusions: Along with Candida albicans, non-albicans candida spp like C. tropicalis, C. krusei, C. glabrata, and C. dubliniensis are increasingly being isolated from clinical samples. CHROM agar is a simple, rapid and inexpensive method for identification of such species. Characterization to species level helps to identify species which might be intrinsically resistant to commonly used antifungal agents.

Isolation and identification of Candida species from urogenital tract infections of women in Baghdad city

2012 ◽  
Vol 36 (0E) ◽  
pp. 115-119
Author(s):  
Mohammed J. Alwan
Keyword(s):  
Pregnant Women ◽  
Candida Species ◽  
Urogenital Tract ◽  
Urine Samples ◽  
Candida Spp ◽  
Isolation And Identification ◽  
Urogenital Infection ◽  
Fungal Isolates ◽  
Vaginal Swabs ◽  
Tract Infections

In order to determine Candida Species isolates that associated with urogenital infection in women,174 vaginal swabs and 66 urine samples were collected from pregnant and non-pregnant women suffering from urogenital tract infection whom visted teaching Al-way hospital during the peroid from January to October 2011. The results showed that 32 out of 174 vaginal swabs were Candida Spp positive (18.3%), high percentage was reported in June and July (25%) for each one and no fungal isolates were recorded in March .The results also explained that high percentage of fungal isolates was recorded in pregnant women (68.7%) as comparing with non-pregnant women (31.2%) .Thirty –two fungal isolates were recorded which included : C.albicans(43.7%); C.tropicals(21.8%), C.prarapsilosis(12.5%) ;C.glabrata (9.3%);C. guilliermondii(6.2%); C. capitatum and C. krusei (3.1%) for each one . Also the study revealed that 9 out 66,(13.6%) of the urine samples were Candida spp positive which included : C.albicans was form high percentage (7.5%) followed by C.tropicals(4.5%) and C.glabrala (1.5%), these results indicated that albicans and non-albicans Candida may be associated with urogenital infection in women.

scholarly journals Effectiveness of photodynamic therapy on Candida species isolated from oral samples of children exposed and not exposed to HIV

2016 ◽  
Vol 64 (3) ◽  
pp. 271-279
Author(s):  
Francine Cristina da SILVA ◽  
Luciano Pereira ROSA ◽  
Antonio Luiz Barbosa PINHEIRO ◽  
Cristiane Yumi KOGA-ITO ◽  
Bruno Pereira de ARAÚJO ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Low Power ◽  
Candida Species ◽  
Irradiation Time ◽  
Blue Dye ◽  
Power Laser ◽  
Candida Spp ◽  
Candida Famata ◽  
Hiv Exposed

ABSTRACT Objective: Identify yeast species isolated from unexposed, exposed and HIV-carrier children, and verify the effectiveness of low power laser photodynamic therapy (PDT) on the yeasts species belonging to the Candida genus. Methods: Fifty children assisted by the Public Health Program of the city of Vitória da Conquista, Bahia, were selected and divided into three groups: unexposed to HIV, exposed to HIV during pregnancy, and HIV-carrier. Saliva samples were collected in a disposable sterile universal container and were plated to Sabouraud dextrose agar supplemented with 0.1 mg/mL chloramphenicol. The plates were incubated at 37°C for 48 h. Three strains of each patient were identified by using an API 20 C AUX system. The strains were submitted to photodynamic therapy (PDT) with a 660 nm low power laser and methylene blue dye at different times of irradiation (90, 180 and 282 sec.). Results: The results showed that the most prevalent species was Candida albicans followed by Candida famata (second most prevalent in unexposed to HIV and HIV carriers)) and Candida parapsilosis (second most prevalent in exposed to HIV group). The CFU/mL of Candida spp. decreased significantly (p<0,05) in all groups treated with PDT compared to the controls. Photodynamic therapy treatments at different exposure times (e.g., PS+L90+, PS+L180+, PS+L282+) revealed that the exposure time of 282 sec. gave the highest reduction of the mean logarithmic CFU/mL. Conclusion: Candida albicans was the most prevalent Candida species in these three groups and Candida non-albicans species, when combined, amounted to a significant percentage of Candida isolates. Photodynamic therapy was effective in inactivating the Candida spp. isolated from the oral cavity of children not exposed to HIV, exposed to HIV and HIV-carriers, with the best photodynamic therapy irradiation time being 282 sec.

scholarly journals Evaluation of the frequency of Candida spp. in hospitalized and non-hospitalized subjects

2018 ◽  
Vol 78 (4) ◽  
pp. 644-652 ◽  
Author(s):  
J. N. Vieira ◽  
A. M. Feijó ◽  
M. E. Bueno ◽  
C. L. Gonçalves ◽  
R. G. Lund ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Teaching Hospital ◽  
Candida Species ◽  
Hospitalized Patients ◽  
The Other ◽  
Medical Clinic ◽  
Candida Spp ◽  
Sabouraud Dextrose Agar ◽  
Peptone Water

Abstract The aim of this study was to evaluate the frequency of Candida species between a non-hospitalized and a hospitalized population. For this purpose, samples of saliva were sampled through sterile swabs, moistened in peptone water and rubbed in the oral cavity of 140 individuals, from which, 70 were hospitalized patients from the Medical Clinic of a Teaching Hospital and the other 70 were non-hospitalized subjects. All saliva samples were plated in Sabouraud Dextrose agar added with Chloramphenicol and incubated at 36 °C for 48 hours. The morphology identification was performed through macroscopic and microscopic characterization, the CHROMagar Candida medium and the VITEK® system Yeast Biochemical Card (bio Mérieux SA, France). The results showed a colonization of Candida spp. in 85.7% the hospitalized individuals, where the species found were C. albicans (60%), C. tropicalis (23.4%), C. krusei (3.3%) and Candida spp. (13.3%). In the non-hospitalized individuals the colonization by Candida spp was 47.1%, and the species found were: C. albicans (45.5%), C.krusei (9.1%), C. guilliermondii (9.1% %), C. tropicalis (3.0%), C. famata (3.0%) and Candida spp. (30.3%). In spite of their presence in oral cavity in both groups, Candida spp. was more frequently isolated in hospitalized individuals, who were 6.73 times more likely to have this fungus in the oral cavity and were 3.88 times more likely to have Candida albicans.

scholarly journals Evaluation of the New Chromogenic Medium Candida ID 2 for Isolation and Identification of Candida albicans and Other Medically Important Candida Species

2006 ◽  
Vol 44 (9) ◽  
pp. 3340-3345 ◽  
Author(s):  
E. Eraso ◽  
M. D. Moragues ◽  
M. Villar-Vidal ◽  
I. H. Sahand ◽  
N. Gonzalez-Gomez ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Isolation And Identification ◽  
Chromogenic Medium

scholarly journals Prevalence of different fungal species in Saliva and Swab samples of patients undergoing Radiotherapy for Oral Cancer

2021 ◽  
Author(s):  
Pratima Putta ◽  
Kannan Natarajan ◽  
Swapna Sridevi Beeraka ◽  
Rakesh Kumar Manne ◽  
Prathi Venkata Sarath
Keyword(s):  
Candida Albicans ◽  
Oral Cancer ◽  
Oral Cavity ◽  
Candida Species ◽  
Fungal Species ◽  
Morbidity And Mortality ◽  
High Prevalence ◽  
Before And After ◽  
Candidal Species

Oral cancer is one of the leading causes of morbidity and mortality globally. There is a lack of studies on the growth of different Candida species in swab pre and post-radiotherapy. Hence, this study aimed to determine different Candida species in the oral cavity of patients who undergo radiotherapy. The study included 50 patients with oral cancer scheduled to undergo radiotherapy. Both saliva and swab samples were collected from all the patients before and after two- and six weeks of starting the radiotherapy. Similar inoculation was performed using the salivary swabs and incubated aerobically at 37°C for 48h. After 48h, culture plates were removed from the incubator and observed for the growth of different fungal species based on the color of the colonies. The culture of samples from patients with oral cancer resulted in the growth of 68% of Candida species in saliva and 44% in swab before initiating radiation. Unlike baseline findings, Candida albicans were identified in a higher number in swab samples (44%) than saliva (24%) post two weeks of radiotherapy. All saliva samples were positive to candida species, in which Candida albicans shows high prevalence (24%). After six weeks of radiotherapy, in the swab, 30% of samples were positive for Candida albicans, and 18% of samples were positive for non-albicans species. Before radiotherapy, at two weeks and six weeks of radiotherapy, saliva samples showed a higher prevalence of Candidal species compared to swab samples. The combination of other Candida species was also higher in saliva samples compared to swab samples during radiotherapy.

scholarly journals Changing Trends of Candida Species and Antifungal Susceptibility Profile of Candida Bloodstream Isolates: A 5-Year Retrospective Survey

2022 ◽  
Vol 14 (12) ◽  
Author(s):  
Ahmet Cem Yardimci ◽  
Dilek Arman
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
Bloodstream Infections ◽  
Antifungal Resistance ◽  
Hemopoietic Stem Cell ◽  
Candida Spp ◽  
Study Results ◽  
Significant Difference

Background: Candida species have emerged as one of the most common causes of bloodstream infections (BSIs). There are limited data on the distribution of Candida spp. and susceptibility by year. Objectives: In this study, we analyzed changes in the distribution of Candida spp. and their antifungal susceptibility profiles from blood cultures. Methods: Records from January 2016 to December 2020 were obtained from the microbiology laboratory in Istanbul. Antifungal susceptibility tests were performed using the VITEK 2 compact system and evaluated according to EUCAST breakpoints. A total of 241 unique candidemia episodes were included in this study. Results: Candida albicans was the predominant pathogen (n = 95, 39.42%), followed by C. parapsilosis (n = 82, 34.02%), C. glabrata (n = 18, 7.47%), C. tropicalis (n = 17, 7.05%), C. krusei (n = 15, 6.22%), and other Candida spp. (n = 14, 5.79%). There was no statistically significant difference in the percentage of episodes of Candida spp. After data analysis, a tendency to shift from C. albicans to C. parapsilosis was observed in the period analyzed in this study. Candida albicans was the most common species in intensive care units (ICUs), hematology and hemopoietic stem cell transplantation units, and surgical clinics, with C. parapsilosis predominant in medical clinics. In general, micafungin susceptibility was the highest, and fluconazole was the lowest. There was reduced sensitivity to fluconazole and voriconazole for C. albicans and C. parapsilosis over 5 years. Conclusions: Detecting changes in the distribution of Candida spp. and antifungal susceptibility over time will lead to the selection of appropriate empirical therapy and monitor phenomena of antifungal resistance. Empirical treatment with antifungal agents is associated with high costs, toxicities, and risk of antifungal resistance. Therefore, it is mandatory to determine and monitor Candida spp. and antifungal susceptibility testing to select appropriate antifungal agents.

Synthesis and Antifungal Activity Against of Candida Species for Some New Heterocyclic Compounds Containing Schiff Base , Oxazepine , Indoline or Imidazolo Units and Their Spectral Characterization

2018 ◽  
pp. 273
Author(s):  
Shaima Ibraheem chyad AL-khazraji ◽  
Bari L. Mohammed ◽  
Siham Sh. AL-Salihi
Keyword(s):  
Schiff Base ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Candida Species ◽  
Chloroacetic Acid ◽  
Candida Krusei ◽  
Phenacyl Bromide ◽  
Pyridine Derivative ◽  
Candida Spp

       The objective of this study is to test In Vitro the twenty chemical compounds that contains Schiff base or oxazepine, indoline, imidazolo units in concentrations( 50, 100, 150) mg / dl as antifungal activity, against three pathogenic Candida species  that occur in humans. We tested one isolates of    ) Candida albicans ,Candida glabrata and Candida krusei). All these species affect human health . The study was carried out in the Laboratory of Public Health , directly of health for the period from May 2016 to April 2017  , Candida spp isolates used in this study were collected from patients admitted at  some private clinical in  Kirkuk city . All isolates were identified using CHROM agar and stored at -70 0C. Preparation of Schiff base ( 1-7 ) from amino pyridine derivatives with aromatic aldehyde by nucleophilic addition reactions preparation of 1,3 – oxazepine 4,7 – dione ( 8-13 ) were carried out by cyclization of appropriate Schiff bases with malic anhydride and phthalic anhydride , preparation of compounds ( 14 – 17) from reaction of a mixture aminopyridine derivative with potassium hydroxide then chloroacetic acid was  added , preparation of compounds ( 18 – 20 ) from reaction of amino pyridine derivative and 4- phenyl phenacyl bromide , all these compounds were characterized by melting points and FT.IR spectroscopy. Some of them were characterized by H1- NMR and C13-NMR spectroscopy .Some compounds contain Schiff base  group in compounds ( 1-7) showed inhibitory effect Candida albicans and Candida krusei  .This study demonstrates that the three Candida species were resistant to a range of compounds (8-13) containing oxazepine and as antifungal against , while the compound(14-17) contain Indolin show low inhabition zon for Candida albicans and the compound (18-20) contain imidazo group showed inhibited  effect against three Candida species.

scholarly journals The Glucocorticoid PYED-1 Disrupts Mature Biofilms of Candida spp. and Inhibits Hyphal Development in Candida albicans

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1396
Author(s):  
Anna Esposito ◽  
Antonella Migliaccio ◽  
Vita Dora Iula ◽  
Raffaele Zarrilli ◽  
Annalisa Guaragna ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Candida Species ◽  
Public Health Problem ◽  
Global Public Health ◽  
Candida Spp ◽  
Abiotic Surfaces ◽  
Candida Infections ◽  
Fungistatic Effect ◽  
Global Public Health Problem

Invasive Candida infections have become a global public health problem due to the increase of Candida species resistant against antifungal therapeutics. The glucocorticoid PYED-1 (pregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1) has antimicrobial activity against various bacterial taxa. Consequently, it might be considered for the treatment of Candida infections. The antifungal activity of PYED-1 was evaluated against several fungal strains that were representative of the five species that causes the majority of Candida infections—namely, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. PYED-1 exhibited a weak antifungal activity and a fungistatic effect on all five Candida species. On the other hand, PYED-1 exhibited a good anti-biofilm activity, and was able to eradicate the preformed biofilms of all Candida species analyzed. Moreover, PYED-1 inhibited germ tube and hyphae formation of C. albicans and reduced adhesion of C. albicans to abiotic surfaces by up to 30%.

scholarly journals ISOLATION, IDENTIFICATION AND ANTIFUNGAL SUSCEPTIBILITY TESTINGOF CANDIDA ISOLATES FROM VARIOUS CLINICAL SPECIMENS AT A TERTIARY CARE HOSPITAL, WESTERN RAJASTHAN

2021 ◽  
Vol 9 (11) ◽  
pp. 809-814
Author(s):  
Sandeep Arora ◽  
◽  
Smita Kulshreshtha ◽  
Usha Verma ◽  
Prameshwar Lal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Antifungal Agents ◽  
Large Population ◽  
Antifungal Susceptibility ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Candida Spp ◽  
Clinical Specimens

Background: Candida spp is a member of the normal flora of the skin, mucous membrane and gastrointestinal tract. Candida continues to be leading cause of morbidity and mortality in large population of immunocompromised and hospitalized patients. Invasive Candidiasis due to non-albicans candida has been on the rise in last few years. This study aims to Spectate Candida using chromogenic medium.The emerging pathogens are resistant to conventional antifungal therapy. Objective: To identify the various species of candida isolated from different clinical specimens and to compare the susceptibility pattern of these isolated species towards different antifungal agents. Methods: All Candida isolates recovered from various clinical samples during the period from September 2017 and august 2018 were studied., These isolates were subjected to grams stain, germ tube test and inoculation on commercially available CHROM agar (HiMedia India). Results: A total of 155 Candida species were isolated from the different clinical specimens of suspected candida infection cases. Most of the isolates obtained were from urine samples 93 (60%) followed by blood 26(16.77%). Non albicans Candida were isolated at a higher rate 101 (65.16%) than Candida albicans 54 (34.84%) . Among 101 non C. albicans, C. tropicalis 55 (35.48%) was the most common species followed by 19 (12.26%) C. parapsilosis. Among all species of Candida commonest isolate was C. tropicalis 55(35.48%) followed by C. albicans 54(34.83%). Candida species from various samples were high resistant to itraconazole (72.26%) followed by fluconazole(70.92%), voriconazole (68.39%) and ketoconazole (57.42%)while there was minimum resistance to amphotericin-B (20%).This study emphasizes the need for monitoring local epidemiologic data and antifungal susceptibility pattern of candida isolates for proper treatment. Conclusions: Along with Candida albicans, non-albicans candida spp like C. tropicalis, parapsilosis, C. krusei and C. glabrata are increasingly being isolated from clinical samples. CHROM agar is a simple, rapid and inexpensive method for identification of such species. Characterization to species level helps to identify species which might be intrinsically resistant to commonly used antifungal agents.

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