scholarly journals GC-MS Analysis of Phytocomponents in the Various Extracts of Shorea robusta Gaertn F.

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Vashisht Sushma ◽  
Singh Manish Pal ◽  
Chawla Viney
Keyword(s):  
Ethanol Extract ◽  
Chloroform Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Shorea Robusta ◽  
Ms Analysis ◽  
Crude Extracts ◽  
Soxhlet Apparatus ◽  
Different Types ◽  
Qualitative Determination

The current investigation was carried out to determine the possible phytocomponents present in the different extracts of Shorea robusta using gas chromatography-mass spectrometry (GC-MS). The dried powdered resin of Shorea robusta was extracted exhaustively by Soxhlet apparatus with different solvents such as methanol, ethanol and chloroform. The prepared extracts were analyzed by GC-MS to identify and characterize the phytocomponents present in the crude extracts. Qualitative determination of different phytocomponents from crude extracts of Shorea robusta using GC-MS revealed different types of high and low molecular weight phytoconstituents with varying quantities present in each of the extracts. The GC-MS analysis provided a variety of peaks determining the presence of different compounds in various extracts of Shorea robusta namely Caryophyllene (1.50%), Caryophylline oxide (6.65%), Ledene oxide (11.17%), Calarene epoxide (5.15%), Alloaromadendrene oxide-(1) (8.72%), Beta-amyrin (7.99%), Alpha-amyrin (1.40%), Cycloisolongifolene (2.54%), Isolongifolene (4.73%), Silane (2.64%). The three extracts possess major phytoconstituents that were identified and characterized spectroscopically. The abundance of phytoconstituents was found to decrease in the order: methanol extract>ethanol extract>chloroform extract.

Preliminary phytochemical screening, antimicrobial potentials and GC-MS analysis of Secamone afzelli Rhoem (Asclepiadaceae) leaves extracts

2020 ◽  
pp. 3728-3738
Author(s):  
Oladapo E. Oyinloye ◽  
Olumuyiwa. S. Alabi
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Soxhlet Apparatus ◽  
Ethanol Extracts ◽  
Tract Infections ◽  
Gastro Intestinal Tract

Secamone afzelli is used ethno-medicinally as remedies for stomach, colic and gastro-intestinal tract infections. This study screened the hexane, ethyl acetate and ethanol extracts of S. afzelli leaves for phytocompounds, antimicrobial activity and identified bioactive compounds in the most active extract using Gas Chromatography Mass Spectrometry (GC-MS) analysis. S. afzelli (200mg) leave was extracted by soxhlet apparatus using three solvents (hexane, ethyl acetate and ethanol) and the phytochemicals determined. Antimicrobial susceptibility and minimum inhibitory concentrations (MICs) was determined by agar-diffusion and agar-dilution methods respectively against selected resistant bacterial and fungal clinical isolates. The most active of the three crude extracts was analysed by GC-MS for bioactive compounds identification. Presence of carbohydrates, anthraquinone, glycosides, proteins, tannin, phenolic compounds and steroids were determined. The three extracts (hexane, ethyl acetate, and ethanol extracts) had antibacterial (Range of inhibition zone = 10 to 18 mm and MICs 12.5 to 200 mg/mL) but no antifungal activity. The ethanol extract was the most active with 29 bioactive compounds including piperazine, phytol, leucine methyl ester, N-methyl-N-(1-methylethyl)- 1Pentanamine, 7-propyl-pyrrolizin-1-thione and 4,5-dimethylnonane. The antibacterial activity and arrays of bioactive compounds detected in S. afzelli leaves suggest that the plant may be a good source of novel antimicrobial compounds.

Development of a New Method for Determination of the Oil Content from Microalgae Lipid Fraction

2017 ◽  
Vol 68 (4) ◽  
pp. 671-674
Author(s):  
Ana Maria Galan ◽  
Ioan Calinescu ◽  
Elena Radu ◽  
Elena Emilia Oprescu ◽  
Gabriel Vasilievici ◽  
...  
Keyword(s):  
Sunflower Oil ◽  
Oil Content ◽  
Lipid Fraction ◽  
New Method ◽  
Ms Analysis ◽  
Qualitative Determination ◽  
Tga Analysis

The purpose of this study was to develop a method for rapid quantitative and qualitative determination of the oil from microalgae lipid fraction obtained from Nannochloris sp biomass. The lipid fraction was first refluxed with 4% KOH in MeOH (60, 90, 120 min), followed by reaction with 20% BF3 in MeOH, using different times of reflux (90,120, 150 min) for each time of reflux with 4% KOH in MeOH. The FAME samples were analyzed by GC-MS analysis. 120 min reflux with 4% KOH in MeOH, 90 min with 20% BF3 in MeOH and a ratio lipid fraction: 4% KOH in MeOH: 20% BF3 in MeOH=1:20:27, were required to obtain the higher percent of oil in the microalgae lipid fraction. The relevance of the method developed was proved by TGA analysis and by transesterification of a sunflower oil sample in the same conditions.

Characterization of Different Types of Biomass Wastes Using Thermogravimetric and ICP-MS Analyses

2020 ◽  
Vol 70 (12) ◽  
pp. 4594-4600
Keyword(s):  
Thermogravimetric Analysis ◽  
Food Industry ◽  
Wood Waste ◽  
Biomass Ash ◽  
Minor Elements ◽  
Ms Analysis ◽  
Icp Ms ◽  
Different Types

The purpose of this study was to characterize some types of biomass wastes resulted from different activities such as: agriculture, forestry and food industry using thermogravimetric and ICP-MS analyses. Also, it was optimized an ICP-MS method for the determination of As, Cd and Pb from biomass ash samples. The ICP-MS analysis revealed that the highest concentration of metals (As, Cd, Pb) was recorded in the wood waste ash sample, also the thermogravimetric analysis indicated that the highest amount of ash was obtained for the same sample (26.82%). The biomass wastes mentioned in this study are alternative recyclable materials, reusable as pellets and briquettes. Keywords: ash, biomass, ICP-MS, minor elements, TG

GC-MS analysis and Mitochondrial Functionality Potential of the Fruits ofTetrapleura tetraptera By Cupric Reducing Antioxidant Capacity Assay

2020 ◽  
Vol 19 (1) ◽  
pp. 338-347
Author(s):  
Chinwe S. Alaribe ◽  
Akolade R. Oladipupo ◽  
Osamede Ojo-Nosakhare ◽  
Omotayo Kehinde ◽  
Adeniyi S. Ogunlaja
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
Antioxidant Capacity ◽  
Vaccenic Acid ◽  
Octadecenoic Acid ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Degenerative Disorders ◽  
Tetrapleura Tetraptera

The use of antioxidants has emerged as a promising therapeutic strategy for the management of mitochondrial dysfunction and other oxidative stress-related degenerative pathologies. Tetrapleura tetraptera is a well-known tree and its fruit is applied traditionally as spice and in the preparation of remedies for different ailments. In this study, the gas chromatography-mass spectrometry (GC-MS) analysis and mitochondrial functionality potential of T. tetraptera fruits were investigated. GC-MS was used to detect compounds in then-hexane and ethanol extracts of T. tetraptera fruits. Cupric reducing antioxidant capacity (CUPRAC) assay was used to evaluate the mitochondrial functionality potential of the ethanol extract. GC-MS analysis revealed the presence of six compounds in then-hexane extract of T. tetraptera fruits. These compounds were detected in trace quantities, the most abundant being 2,3-dimethyl-3-buten-2-ol (0.04%). The compounds: cis-vaccenic acid (35.37%), n-hexadecanoic acid (28.09%), 6-octadecenoic acid (25.21%), and octadecanoic acid (11.33%) were identified in the ethanol extract of the fruits. Consequently, the ethanol extract was subjected to CUPRAC assay. The ethanol extract exhibited a concentration-dependent high cupric reducing capacity, returning CUPRAC values in the range of 0.090 to 0.403 at the concentration of 10 – 80 μg/ml. This activity was comparable to that of the positive control, naringenin, which showed CUPRAC values of 0.059 – 0.378 at the same concentrations. These results indicate that T. tetraptera fruits possess good antioxidant property as evaluated by other related antioxidant assays. This could be attributed to a synergistic effect of the phytochemical constituents. Hence consumption of T. tetraptera fruits could be beneficial for the prevention of mitochondrial dysfunction and other oxidative stress-related degenerative disorders. Keywords: Tetrapleura tetraptera, mitochondrial dysfunction, degenerative disorders, CUPRAC, GC-MS, Nigeria

scholarly journals Determination of Volatile Compounds in Nut-Based Milk Alternative Beverages by HS-SPME Prior to GC-MS Analysis

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3091 ◽  
Author(s):  
Natalia Manousi ◽  
George A. Zachariadis
Keyword(s):  
Volatile Compounds ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Ms Analysis ◽  
Salt Addition ◽  
Compositional Changes ◽  
Optimum Extraction ◽  
Technological Treatment

A reliable Headspace-Solid Phase Microextraction (HS-SPME) method was developed for the determination of polar volatile components of commercial nut-based milk alternative drinks prior to Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Under the optimum extraction conditions, a divinylbenzene (DVB)/Carboxen™ CAR)/polydimethylsiloxane (PDMS) fiber was used and 2 mL of sample was heated at 60 °C for 40 min under stirring, without salt addition. Ten compounds from different chemical classes (heptane, a-pinene, toluene, 2-methylpyrazine, 3-heptanone, heptanal, 2-octanone, 1-heptanol, benzaldehyde and 1-octanol) were chosen as model analytes for quantification. Limits of detection and limits of quantification were found to be 0.33–1.67 ng g−1 and 1–5 ng g−1, accordingly. Good linearity, precision and accuracy were obtained as well as a wide linear range. The proposed method was successfully applied to various beverages including almond milk, walnut milk, peanut milk and almond chocolate milk. More than 70 volatile compounds were detected in the different samples. Most of the detected volatiles were aldehydes, ketones and alcohols. This technique can be used for the determination of volatile compounds in nut-based beverages, to detect compositional changes during storage and technological treatment used for their production.

Comparative Studies of Chemical Constituents of Hibiscus rosa-sinensis Using Different Extraction Techniques

2020 ◽  
Vol 981 ◽  
pp. 296-301
Author(s):  
Nur Salsabila Ahmad Roslan ◽  
Seema Zareen ◽  
Normaiza Zamri
Keyword(s):  
Essential Oil ◽  
Bioactive Compounds ◽  
Capillary Column ◽  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hibiscus Rosa Sinensis ◽  
Soxhlet Apparatus ◽  
The Family

Hibiscus rosa-sinensis which belongs to the family Malvaceae, is a national flower of Malaysia. This glabrous tree can usually grow around 1 to 3 meters tall. The objective of present study was to categorize the essential oil constituent of flowers and leaves extract using different extraction method namely Soxhlet and hydrodistillation. The essential oil obtained from Soxhlet (10.75%) and hydrodistillation (11.40%) were subjected to Gas Chromatography-Mass Spectrometry (GC-MS) for determination of bioactive compounds. The GC-MS analysis was carried out by Agilent 7980A series GC instrument and DB-1MS capillary column with the dimensions of 30 m × 0.25 mm. A total of 21 and 20 compounds were identified for Soxhlet and hyrodistillation technique, respectively. Major compound found in essential oil for Soxhlet apparatus method was (Z,Z,Z)-9-12,15-Octadecatrienoic acid, methyl ester (23.59%) while for hydrodistillation was 1-Methylene-2b-hydroxymethyl-3,3-dimethyl-4b-(3-methylbut-2-enyl)-cyclohexane (16.57%). The presence of numerous bioactive compounds provides insights to the potential of the Hibiscus rosa-sinensis in pharmaceutical industry.

BRINE SHRIMPS LETHALITY TEST OF ETHANOL EXTRACT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE FRACTION OF BLIGHIA SAPIDA

2020 ◽  
pp. 208-212
Author(s):  
ADEKOLA MB ◽  
AREOLA JO ◽  
ORIYOMI OV ◽  
APATA JT ◽  
APALOWO OE ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Blighia Sapida

Objective: The objective of the study was to evaluate brine shrimp lethality of Blighia sapida stem-bark extract and its fractions and identify the bioactive constituents in the ethyl acetate fraction (EAF) using gas chromatography-mass spectrometry (GC-MS) technique. Methods: The ethanol extract (EE) and its fractions were subjected to lethality assay, and GC-MS analysis of EAF was carried out. Results: The lethality test showed a concentration-dependent mortality rate in the brine shrimp nauplii for the EE and its fractions. GC-MS analysis of EAF of the extract revealed the existence of 13 peaks of the GC-MS chromatogram with only one prominent compound, n-hexadecanoic acid (peak area of 10.13%). Conclusion: The result revealed the presence of 13 bioactive components in the EAF of the extract, the majority of which have been reported for different biological activities, hence, justifies the use of the plant in the treatment and management of different diseases ethnomedicinally.

Identification of Secondary Metabolites from the Ethanol extract of the leaves of Bauhinia tomentosa by GC-MS Analysis

2021 ◽  
pp. 2335-2341
Author(s):  
R. Balabhaskar ◽  
K. Vijayalakshmi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Mass Spectra ◽  
Ethanol Extract ◽  
Alpha Tocopherol ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Gas Chromatography-Mass Spectrometry (GC-MS) is normally used for direct analysis of chemical components existing in herbal medicines. The medicinal plants are having numerous bioactive components which are identified even at less than 1ng by using GC-MS or LC-MS analysis. The aim of this study is to identify the secondary metabolites present in the leaves of B. tomentosa using gas chromatography-mass spectrometry (GC-MS) analysis. In the present study the ethanol extract of the leaves of Bauhinia tomentosa has been subjected to GC-MS analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of 14 secondary metabolites. These compounds were identified by comparing their retention times and peak areas with those from the literature and by interpretation of the mass spectra. The major secondary metabolites were DL-.alpha.-tocopherol (14.84%), 2-[(trimethylsilyl oxy]-, methyl ester, 1-alpha,2-alpha.-epoxy-1-beta-methylcholesta-4,6-dien-3-one (12.93%), pentacosenoic acid (12.71%), phytol (10.28%), Ethyl Isoallocholate (8.197%), Spirost-8-en-11-one-3-hydroxy-,(3-beta,5 alpha,14 beta,20 beta, 22 beta,25R)-(8.162%), Urs-12-en-28-ol (6.675%), 1-Octadecyne (5.702%) and Cholest-8-en-3-beta-ol,Acetate (5.426%). The compounds having area less that 5% were considered of no significance. These findings suggest that the presence of these secondary metabolites may be the cause for the properties exhibited by Bauhinia tomentosa. Thus, presence of various bioactive compounds justifies the use of the leaf for various ailments by traditional practitioners.

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