scholarly journals Biosintesis dan Karakterisasi Selulosa Bakteri menggunakan Media Sari Pedada (Sonneratia caseolaris) dan Kundur (Benincasa hispida)

2019 ◽  
Vol 9 (01) ◽  
pp. 1
Author(s):  
Dian Fitriarni ◽  
Irianto Sastro Prawiro ◽  
Nenengsih Verawati ◽  
Wedi Hardiansyah ◽  
Dwi Aprianti

Selulosa bakteri merupakan satu produk yang telah banyak diaplikasikan pada bidang medis, pangan, dan bahkan dapat menjadi bahan baku alternatif untuk mengganti beberapa jenis produk berbahan baku selulosa tumbuhan. Banyak faktor yang berpengaruh terhadap kualitas selulosa bakteri yang dihasilkan seperti bahan baku dan sumber nitrogen untuk media fermentasi. Penelitian ini bertujuan untuk mengetahui karakteristik selulosa bakteri dari hasil fermentasi menggunakan dua media dari sari buah pedada dan kundur dengan berbagai sumber nitrogen berbeda. Sari buah kundur dan sari buah pedada memiliki karakteristik berbeda sehingga mempengaruhi formulasi bahan yang digunakan. Kedua media yang digunakan dimodifkasi dengan penambahan berbagai sumber nitrogen untuk melihat bagaimana karakteristik selulosa bakteri yang dihasilkan. Penelitian dilakukan melalui tahapan eksperimen untuk mendapatkan formulasi yang tepat agar diperoleh ketebalan selulosa maksimal. Berdasarkan hasil karakterisasi selulosa bakteri, berat kering selulosa yang dihasilkan menggunakan media sari buah pedada dengan penambahan urea, yeast, ekstrak kecambah kacang hijau berturut turut 0,37 g/L, 0,52 g/L, dan 2,23 g/L, sedangkan selulosa yang dihasilkan menggunakan media sari buah kundur dengan penambahan urea, yeast, ekstrak kecambah kacang hijau berturut turut 1,3 g/L, 0,32 g/L, dan 5,43 g/L. AbstractBacterial cellulose is a product that has been widely applied in the medical, food, and even alternative raw materials for replacing several types of products made from plant cellulose. Many factors influence the quality of bacterial cellulose produced such as raw materials and nitrogen sources for fermentation media. This study aims to determine the characteristics of bacterial cellulose from fermentation using two different media of pedada juice and gourd with various nitrogen sources. Kundur fruit juice and pedada fruit juice have different characteristics so that affect the formulation of the ingredients used. The two media used were modifed by adding various nitrogen sources to see how the characteristics of bacterial cellulose produced. The research was carried out through experimental stages to obtain the right formulation to produce maximum cellulose thickness. Based on the characterization result of bacterial cellulose, the dry weight obtained from the use of pedada fruit juice media using urea, yeast, green bean was in the amount of 0.37 g/L, 0.52 g/L, and 2.23 g/L, respectively. On the other hand bacterial cellulose produced from the use of kundur fruit juice media with the same nitrogen source was obtained with the dry weight of 1.38 g/L, 0.32 g/L, and 5.43 g/L, respectively. Keywords: bacterial cellulose, pedada, kundur, yeast, green bean  

2013 ◽  
Vol 821-822 ◽  
pp. 1093-1097
Author(s):  
Yin Chuan Chen ◽  
Yan Bin Zhu ◽  
Yong Lin ◽  
Jun Wang

As a novel nanofunctional material, bacterial cellulose (BC) has been widely applied in food, papermaking, medical, spinning, acoustics, and chemical fields owing to the high purity, the nanoscale fine network structure and etc. The production of BC is significantly affected by nutrients such as carbon and nitrogen sources. Particularly, the output, form and structure of BC are affected by ethanol, organic acids and other chemical substances. Cheaply available raw materials, including fruits, molasses and other natural ones, are emphasized in this review.


Cellulose ◽  
2021 ◽  
Author(s):  
Katri S. Kontturi ◽  
Koon-Yang Lee ◽  
Mitchell P. Jones ◽  
William W. Sampson ◽  
Alexander Bismarck ◽  
...  

Abstract Cellulose nanopapers provide diverse, strong and lightweight templates prepared entirely from sustainable raw materials, cellulose nanofibers (CNFs). Yet the strength of CNFs has not been fully capitalized in the resulting nanopapers and the relative influence of CNF strength, their bonding, and biological origin to nanopaper strength are unknown. Here, we show that basic principles from paper physics can be applied to CNF nanopapers to illuminate those relationships. Importantly, it appeared that ~ 200 MPa was the theoretical maximum for nanopapers with random fibril orientation. Furthermore, we demonstrate the contrast in tensile strength for nanopapers prepared from bacterial cellulose (BC) and wood-based nanofibrillated cellulose (NFC). Endemic amorphous polysaccharides (hemicelluloses) in NFC act as matrix in NFC nanopapers, strengthening the bonding between CNFs just like it improves the bonding between CNFs in the primary cell wall of plants. The conclusions apply to all composites containing non-woven fiber mats as reinforcement. Graphic abstract


1992 ◽  
Vol 73 (4) ◽  
pp. 1291-1296 ◽  
Author(s):  
L. E. Olson ◽  
R. L. Wardle

We examined whether wedging a catheter (0.5 cm OD) into a subsegmental airway in dog (n = 6) or pig lungs (n = 5) and increasing pressure in the distal lung segment affected pulmonary blood flow. Dogs and pigs were anesthetized and studied in the prone position. Pulmonary blood flow was measured by injecting radiolabeled microspheres (15 microns diam) into the right atrium when airway pressure (Pao) was 0 cmH2O and pressure in the segment distal to the wedged catheter (Ps) was 0, 5, or 15 cmH2O and when Pao = Ps = 15 cmH2O. The lungs were excised, air-dried, and sectioned. Blood flow per gram dry weight normalized to cardiac output to the right or left lung, as appropriate, was calculated for the test segment, a control segment in the opposite lung corresponding anatomically to the test segment, the remainder of the lung containing the test segment (test lung), and the remainder of the lung containing the control segment (control lung). The presence of the catheter reduced blood flow in the test segment compared with that in the control segment and in the test lung. Blood flow was not affected by increasing pressure in the test segment. We conclude that, in studies designed to measure collateral ventilation in dog lungs, the presence of the wedged catheter is likely to have a greater effect on blood flow than the increase in pressure associated with measuring collateral airway resistance.


1981 ◽  
Vol 50 (1) ◽  
pp. 102-106 ◽  
Author(s):  
P. S. Barie ◽  
T. S. Hakim ◽  
A. B. Malik

We determined the effect of pulmonary hypoperfusion on extravascular water accumulation in anesthetized dogs by occluding the left pulmonary artery for 3 h and then reperfusing it for 24 h. The lung was reperfused either at normal left atrial pressure (Pla) or during increased Pla induced by a left atrial balloon. In each case the extravascular water content-to-bloodless dry weight ratio (W/D) of the left lung was compared with that of the right lung. The W/D of the left lung of 3.26 +/- 0.49 ml/g was not significantly different from the value of 2.87 +/- 0.37 for the right lung after the reperfusion at normal Pla. However, the W/D of the left lung of 5.10 +/- 0.38 ml/g was greater (P less than 0.05) than the value of 4.42 +/- 0.34 for the right lung after reperfusion at Pla of 25 Torr. This difference could not be prevented by pretreatment with heparin, suggesting that the increase in lung water content was not due to activation of intravascular coagulation secondary to stasis occurring during the occlusion. Because the left lung was more edematous than the right one, even though both lungs had been subjected to the same increase in Pla, the results suggest that a period of pulmonary hypoperfusion causes an increase in the interstitial protein concentration.


2013 ◽  
Vol 37 (4) ◽  
pp. 869-876 ◽  
Author(s):  
Altanys Silva Calheiros ◽  
Mario de Andrade Lira Junior ◽  
Débora Magalhães Soares ◽  
Márcia do Vale Barreto Figueiredo

Biological nitrogen fixation by rhizobium-legume symbiosis represents one of the most important nitrogen sources for plants and depends strongly on the symbiotic efficiency of the rhizobium strain. This study evaluated the symbiotic capacity of rhizobial isolates from calopo (CALOPOGONIUM MUCUNOIDES) taken from an agrisoil under BRACHIARIA DECUMBENS pasture, sabiá (MIMOSA CAESALPINIIFOLIA) plantations and Atlantic Forest areas of the Dry Forest Zone of Pernambuco. A total of 1,575 isolates were obtained from 398 groups. A single random isolate of each group was authenticated, in randomized blocks with two replications. Each plant was inoculated with 1 mL of a bacterial broth, containing an estimated population of 10(8) rhizobial cells mL-1. Forty-five days after inoculation, the plants were harvested, separated into shoots, roots and nodules, oven-dried to constant mass, and weighed. Next, the symbiotic capability was tested with 1.5 kg of an autoclaved sand:vermiculite (1:1) mixture in polyethylene bags. The treatments consisted of 122 authenticated isolates, selected based on the shoot dry matter, five uninoculated controls (treated with 0, 50, 100, 150, or 200 kg ha-1 N) and a control inoculated with SEMIA 6152 (=BR1602), a strain of BRADYRHIZOBIUM JAPONICUM The test was performed as described above. The shoot dry matter of the plants inoculated with the most effective isolates did not differ from that of plants treated with 150 kg ha-1 N. Shoot dry matter was positively correlated with all other variables. The proportion of effective isolates was highest among isolates from SABIÁ forests. There was great variation in nodule dry weight, as well as in N contents and total N.


1978 ◽  
Vol 41 (5) ◽  
pp. 344-347 ◽  
Author(s):  
O. PENSALA ◽  
A. NISKANEN ◽  
S. LINDROTH

Unsweetened and sweetened (20 and 44% sucrose) black currant, blueberry and strawberry jams with spores of Aspergillus parasiticus NRRL 2999 were incubated at different temperatures and atmospheres for 0.5, 1, 2, and 6 months. Hyphal dry weight, pH of medium and aflatoxin production were examined. Also, the aflatoxin distribution between mold and jam layers was examined in jam with uncontrolled and controlled pH (initial pH 3.1–3.6 and 5.6 respectively) and in 20% yeast extract sucrose broth (initial pH 5.6) after 2 weeks of incubation. Aflatoxin was observed in black currant and strawberry jams stored at 22 and 30 C, but not in blueberry jam. Addition of sugar prevented production of aflatoxin in detectable amounts, although it enhanced fungal growth. Storage at 4 C resulted in a marked reduction in fungal growth. The high CO2 atmosphere prevented production of aflatoxin in detectable amounts in black currant and blueberry jams but not in strawberry jam. Raising the initial pH of the stored jam caused an increase in aflatoxin synthesis, although the amount of fungal mycelium, in contrast was reduced. Aflatoxin synthesis as a function of fungal growth was significantly weaker in the jams than in the yeast extract sucrose broth. The results imply that the jam raw materials, particularly blueberry, contain substances inhibiting production of atlatoxins. Alternatively, it is also possible that the jam materials contain only small amounts of nutrients necessary for synthesis of aflatoxin.


2014 ◽  
Vol 23 (3) ◽  
pp. 236-245 ◽  
Author(s):  
Mauritz Vilhelm Vestberg ◽  
Sanna Kukkonen ◽  
Päivi Parikka ◽  
Dan Yu ◽  
Martin Romantschuk

There is increasing global interest in using compost to suppress soil-borne fungal and bacterial diseases and nematodes. We studied the reproducibility of compost suppressive capacity (SC) against Pythium wilt of cucumber using nine composts produced by the same composting plant in 2008 and 2009. A bioassay was set up in a greenhouse using cucumber inoculated with two strains of Pythium. The composts were used as 20% mixtures (v:v) of a basic steam-sterilized light Sphagnum peat and sand (3:1, v:v). Shoot height was measured weekly during the 5-week experiment. At harvest, the SC was calculated as the % difference in shoot dry weight (DW) between non-inoculated and inoculated cucumbers. The SC was not affected by year of production (2008 or 2009), indicating reproducibility of SC when the raw materials and the composting method are not changed. Differences in shoot height were not as pronounced as those for shoot DW. The results were encouraging, but further studies are still needed for producing compost with guaranteed suppressiveness properties.


Metals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 74
Author(s):  
Christoph Zschiesche ◽  
Jürgen Antrekowitsch

The processing of polymetallic materials provides some challenges to every flowsheet. Within Aurubis Cu-Pb-metallurgical flowsheet, a broad range of raw materials and intermediates are processed. Continuous improvements are required to adapt the flowsheet according to the changing material quantity and quality. Therefore, thermodynamic modeling is the desired and most efficient way to conduct scenario analysis. Hence, databases and software are becoming better and better as the acceptance of this method increased. Further understanding is promoted by conducting experimental test work to validate the calculated results. In this research work, the impact of various oxygen potential on the formation of the condensed phases’ slag, matte, speiss and crude lead were investigated. A frequent check of slag metallurgy, in particular, the iron and lead concentration, provide feedback if the metallurgical process is operating at the right oxygen potential. Following, the calculated distribution coefficients for Cu, Pb, As, Sb, Sn and Ni between matte/speiss and speiss/lead are discussed.


2020 ◽  
Vol 14 (02) ◽  
pp. 143
Author(s):  
Priyanto Priyanto ◽  
Yuli Wibowo ◽  
Jay Jayus

The demand for amplang crackers is now increasing. However, the price of its raw material, mackerel fish, is generally higher compare to other fishes, causing the higher cost of this type crackers production. Effort to explore other fish as an alternative raw material is required to overcome the problems. Catfish is one of the choices to be used as raw material since this fish is more available in a market in a lower price, but the protein properties of this fish is different to mackarel, which will be possible to affect the nature of the crackers obtained. To produce catfish amplang crackers to be similar to common amplang product, it is necessary to find out the right composition of catfish and tapioca flour used. Therefore, the purpose of this research was to characterize physico-chemically and organoleptically, the catfish amplang crackers made under different ratio of fish flesh and starch. The experiment was designed using completely randomized design. Physico-chemical data obtained were analyzed using ANOVA, while the organoleptic test data were examined using chi-square method. The effectiveness test was carried out to find the proper ratio of raw materials composition used. The results showed that the higher the ratio of the fish flesh and the starch composition, the lower the texture value of the crackers as an indication of the decrease of its crispness. Although the lightness of the crackers was lowered by the addition of more starch to the dough, its linear expansion and hygroscopicity were not affected. The results of organoleptic test showed that the panelist was prefer to a higher crispness of crackers. The most preferable cracker was the one with 1:2.25 ratio of catfish flesh and starch, showing the characteristic of 1.80% moisture, 2.08% ash, 5.75% protein, 23.55% fat, and 66,93% carbohydrate. Keywords: cracker, catfish, tapioca starch


2021 ◽  
Vol 4 (1) ◽  
pp. 26
Author(s):  
Zelfi Zakir ◽  
Sri Wahyuni ◽  
Faidil Tanjung

This study aims to analyze the ICN Kerinci Coffee Perfume agroindustry. Data collected from June 2019 to August 2019 were analyzed using the descriptive analysis to describe the business profile and quantitative analysis using the variable costing approach to explore profit and breakeven point. The results showed that the ICN Kerinci coffee perfume business is a medium-scale business with ten workers, including business owners as leaders who concurrently marketers and wives as administrative and financial staff. In fulfilling raw materials in green beans from the Robusta type, business owners collaborate with partner farmers. At the time of research, ICN Kerinci only produced one kind of product with two packaging variants, namely Kerinci original perfume for Rp.22,000/pack (45 grams) and an original variant + oil coffee (package) for Rp.35,000/pack. The study suggested expanding the market, especially to Eastern Indonesia, and increasing good cooperation with farmers to ensure the certainty of supply of raw materials; and provide prices based on sorting and grading to stimulate farmers to improve the quality of their green bean products.


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