Enhancement of 4-Hydroxyisoleucine Content of Trigonella Foenum-Graecum (Fenugreek) by Trichoderma viride

2021 ◽  
Vol 25 (9) ◽  
pp. 133-145
Author(s):  
Haritha Addala ◽  
Lalitha Pappu
Keyword(s):  
Root Length ◽  
Lateral Root ◽  
Growth Parameters ◽  
Trichoderma Viride ◽  
Total Yield ◽  
Growth Yield ◽  
Shoot Length ◽  
Leguminous Plant ◽  
Fenugreek Seeds ◽  
Trigonella Foenum Graecum

Trigonella foenum- graecum (fenugreek) is a leguminous plant with poor nitrogen fixing ability. The current study deals with the effects of T.viride on growth, yield and 4-hydroxyisoleucine content in fenugreek. 4-hydroxyisoleucine is an antidiabetic compound present only in plants particularly in fenugreek. Treatment of fenugreek seeds with T.viride enhanced the growth, yield and 4-hydroxyisoleucine content significantly compared to control. Seeds were treated with T.viride suspension (107spores/seed) and untreated seeds (1 ml distilled water/ seed) served as control sown in pots filled with potting soil. Several growth parameters like root length and lateral root expansion, shoot length, yield, chlorophyll and nitrogen were estimated. 4-hydroxyisoleucine content in fenugreek seeds was estimated by ninhydrin method. A significant increase of 25 % in root length and 128 % in lateral root expansion and 50 % in shoot length was observed in treated plants over control. The total yield increased by 95 % in treated one. The total chlorophyll content exceeded by 21.86 % and total plant nitrogen by 121.73 %. An increase of 27 % of 4-hydroxyisoleucine content is observed in treated fenugreek plants over control.

scholarly journals Detection of Genetic Polymorphisms using Random Amplified Polymorphic DNA (RAPD)-PCR in Fenugreek (Trigonella foenum-graecum) Plants after Seed Treatment with Biotic and Abiotic Agents

2021 ◽  
Author(s):  
Zahraa Abid Nima Al-Yasiri ◽  
Basheer Al-Alwani ◽  
Jawad K. Abood Al-Janabi
Keyword(s):  
Chlorophyll Content ◽  
Genetic Polymorphisms ◽  
Root Length ◽  
Growth Parameters ◽  
Random Amplified Polymorphic Dna ◽  
Fenugreek Seeds ◽  
Trigonella Foenum Graecum ◽  
Rapd Pcr ◽  
Trigonella Foenum Graecum L ◽  
Th 1

Trigonella foenum-graecum L. is a widely used herb in traditional medicine. The aim of this study was to evaluate the genetic polymorphisms in fenugreek plants following the treatment of fenugreek seeds with different combinations of biotic and abiotic agents using the random amplified polymorphic DNA (RAPD)-PCR technique. We assessed the effects of two strains of the fungus Trichoderma harzianum (Th-1 and Th-2), methyl jasmonate (MeJA), and Aloe vera gel (AVG) on growth parameters of fenugreek plants. Combinations of Th-1, MeJA, AVG significantly increased fenugreek root length, shoot length, shoot fresh weight, number of true leaves, and chlorophyll content. The Th-2 isolate, on the other hand, markedly slowed plant development (except for root length which was not affected significantly). In contrast, the combination with MeJA had no considerable effect on all growth measures, whereas the combination with VAG resulted in a substantial drop in shoot height and chlorophyll content when compared to other growth parameters that were unaffected. The present study has shown that the PCR amplification of DNA, using five primers for RAPD analysis, produced 62 DNA fragments that could be scored in all genotypes. The total number of polymorphic bands was 26, and the average percentage of polymorphism was 54.21%. The RAPD-PCR results showed that the treatment of fenugreek seeds with Th-1 alone or in combination with MeJA and AVG induced polymorphisms in fenugreek leaves.

scholarly journals INFLUENCE OF CADMIUM ON GROWTH AND BIOCHEMICAL CONTENTS OF TOMATO PLANTS

1970 ◽  
pp. 04-06
Author(s):  
A. Natarajan, P. Vijayarengan ◽  
M. Vijayaragavan
Keyword(s):  
Root Length ◽  
Growth Parameters ◽  
Sandy Loam ◽  
Shoot Length ◽  
Phenol Content ◽  
Tomato Plants ◽  
Biochemical Constituents ◽  
Root And Shoot Length ◽  
Soil Cadmium ◽  
Loam Soil

The increasing concentrations (10, 25, 50, 75 and 100 mg /kg) of soil cadmium on growth and biochemical contents in tomato plants were analysed on 30th sampling days. Control plants were maintained separately. Plants were grown in pots containing 3 kg of air dried sandy loam soil and treated with different concentrations (mg/kg) of cadmium (0, 10, 25, 50, 75 and 100 ). Treatments decreased the growth parameters such as root and shoot length  and biochemical constituents such as, protein,(except, proline and  phenol content) contents in tomato plants compared to untreated plants. The shoot length of cadmium treated tomato plants was higher than the root length. proline and phenol content of root of  tomato plants was higher than the shoot.

HERBICIDAL ACTIVITY OF Ageratum conyzoides AGAINST PARTHENIUM

2020 ◽  
Vol 27 (2) ◽  
pp. 137-146
Author(s):  
Nimra Javaid ◽  
Arshad Javaid ◽  
Mukhtar Hussain Shah ◽  
Iqra Haider Khan ◽  
Syed Muhammad Waleed
Keyword(s):  
Root Length ◽  
Plant Biomass ◽  
Growth Parameters ◽  
Shoot Length ◽  
Herbicidal Activity ◽  
Ageratum Conyzoides ◽  
Stem Extract ◽  
Noxious Weed ◽  
Germination And Growth ◽  
Laboratory Bioassays

This study was carried out to explore herbicidal potential of an asteraceous weed Ageratum conyzoides against an exotic noxious weed parthenium (Parthenium hysterophorus L.). Different concentrations (0, 2, 4, 6, 8 and 10%) of aqueous extracts of various parts of the tested weed were used in laboratory bioassays. ANOVA revealed the significant effect of plant part bioassay and extract concentration on germination as well as various root and shoot growth parameters. Leaf extract was found the most herbicidal where a 10% extract completely inhibited the germination and growth of parthenium. Root and inflorescence extracts were also highly inhibitory in suppressing the germination up to 89% and 95%, shoot length up to 80% and 89%, root length up to 86% and 91%, and plant biomass up to 89% and 98%, respectively. Stem extract showed the least herbicidal activity causing up to 46%, 59%, 73% and 37% reduction in germination, shoot length, root length and plant biomass, respectively. This study concludes that leaf, root and inflorescence extracts of A. conyzoides possess strong herbicidal potential against germination and growth of parthenium.

scholarly journals Bioconversion of Non Edible Vegetables from Market into Biofertilizer for Crop Improvement

2016 ◽  
Vol 8 (6) ◽  
pp. 71
Author(s):  
Geetha Karuppasamy ◽  
Michael Antony D'Couto ◽  
Anant Achary
Keyword(s):  
Plant Growth ◽  
Root Length ◽  
Growth Parameters ◽  
Crop Improvement ◽  
C:N Ratio ◽  
Value Added ◽  
Fold Increase ◽  
Shoot Length ◽  
Germination Index ◽  
Vigour Index

<p>The fruits and vegetables lost due to spoilage in the market can be converted to nutrient rich organic biofertilizer, vermicompost and worm tea. In this study, non-edible vegetables from market [French beans (S1), Lady’s fingers (S2) and Brinjal (S3)] were used for production of vermicompost and worm tea using <em>Eisenia fetida</em> for environmental friendly management and recycling, as value added product for crop production. Vermicomposting was carried out in four bins. Three bins (S1, S2 &amp; S3) consisted of individual pre-digested vegetables and the fourth bin (S4) was set up with a mixture of all three pre-digested vegetables in equal proportions. Physico-chemical properties and essential nutrients like pH, temperature, moisture content, total organic carbon (C), total nitrogen (N), available phosphorus (P) and exchangeable potassium (K) of the pre-digested vegetables and/or compost were analysed at regular time intervals. There was around 6-10 fold increase in earthworm count at the end of vermicomposting. During vermicomposting, the N and P content of the compost showed 2 to 4 fold increase whereas 10 to 15 fold increase in K content was noticed. Low C:N ratio (4 to 9) was obtained for vermicompost VS4. The worm tea (vermi wash) and vermicompost collected were used for plant growth studies on <em>Vigna radiata</em> (Green gram). Growth parameters like germination percentage, vigour index, germination index (GI), shoot length (cm), root length (cm) and leaf length (cm) were studied. There was noticeable improvement in germination % (1.7 fold), vigour index (2.7 fold) and germination index. A 20 fold increase in shoot length was also seen in test plants when compared to control (2 fold). Statistical analysis of various growth parameters like root length and plant height indicated that vermicompost made with waste brinjal has a significant response with p ≤ 0.05. Based on the results obtained, waste brinjal, which is abundantly available locally can be economically converted to organic biofertilizers and used for soil and crop improvement. Through this study, a cost effective and environment friendly method for efficient utilization of market waste vegetables has been proposed for promoting plant growth and development.</p>

scholarly journals Impact of dye effluent on growth and chlorophyll content of Alfalfa (Medicago sativa l.)

2016 ◽  
Vol 5 (10) ◽  
pp. 1432
Author(s):  
Pratibha Mahawar* ◽  
Azra Akhtar
Keyword(s):  
Medicago Sativa ◽  
Seedling Growth ◽  
Root Length ◽  
Growth Parameters ◽  
Research Work ◽  
Life Time ◽  
Shoot Length ◽  
Chlorophyll Contents ◽  
Root And Shoot Length ◽  
The Impact

The textile industry plays an important role in the world economy as well as in our daily life time, it consumes large quantity of water and generates huge amount of waste water. The chemical reagents used in the textile sector are diverse in chemical composition ranging from inorganic to organic molecules. The presence of these chemicals will show detrimental effects on the germination process and growth of seedlings. Present research work has been carried out to study the impact of effluent at different concentrations (20%, 40%, 60%, 80%, 100%) on seed germination and seedling growth of Medicago sativa. On the 14th day of seedling growth, maximum root and shoot length were observed at 20% concentration of effluent i.e. 5.4cm (root length) and 5.1cm (shoot length) which increased 3.8% and 4.0% respectively in comparison to control. At high concentrations of treatment levels root length was decreased 2.04%, 19.2%, 26.5%, 51.0% respectively and shoot length was decreased 3.84%, 17.3%, 26.9%, 44.2% respectively at 40%, 60%, 80% and 100% treatment levels in comparison to control (4.9cm and 5.2cm respectively). Same trend was observed during estimation of dry weight and chlorophyll contents.  Inhibition of seedling germination and seedling growth at higher concentrations of effluent may be due to high level of dissolved solids which inhance the salinity. The present study concluded that the dyeing effluent waste significantly influence growth parameters of Medicago sativa.

scholarly journals Evaluation of Biofertilizers and Biochar on the Plant Growth and Productivity of Soybean (Glycine max)

2021 ◽  
pp. 74-86
Author(s):  
Saiteja Atluri ◽  
Deepshikha Thakur ◽  
Dinesh Bukke ◽  
Naleeni Ramawat
Keyword(s):  
Glycine Max ◽  
Plant Growth ◽  
Root Length ◽  
Growth Parameters ◽  
Uttar Pradesh ◽  
Shoot Length ◽  
Soil Parameters ◽  
Organic Farm ◽  
Number Of Leaves ◽  
Pod Length

The field experiment was conducted in Organic farm of Amity university Noida, Uttar Pradesh, during the kharif season, to evaluate the sole and conjoint effect of biofertilizers and biochar on the yield, growth and productivity of Soybean (Glycine max). The five treatments viz. T1 (Control), T2 (Biochar), T3 (Biochar + Rhizobium), T4 (Biochar + Azospirillum) and T5 (Biochar + Rhizobium + Azospirillum) were used. Plant growth parameters like shoot length, root length, number of leaves, pod length, pod girth, plant height, crop yield and soil parameters were observed for the effect of various treatments. Among the all treatments the treatment T3 (Biochar with Rhizobium) maximum shoot length (52.30), root length (17.30) followed by T4 (Biochar + Azospirillum) and plant productive are maximum mean of number of pods was recorded in T3 (Biochar + Rhizobium) i.e. 32.6, whereas minimum (30.1) was recorded in T1(control) followed by T4(Biochar + Azospirillum) i.e.32.41, T5 (Biochar + Rhizobium + Azospirillum) i.e. 31.05 and T2 (Biochar) i.e.31.31   has shown significant effect on plant growth characters and plant yield. These results indicate that the   conjoint use of biochar and Rhizobium have potential to enhance the crop performance and simultaneously improves the soil properties for sustainable farming without reliance on synthetic agrochemicals.

Growth and Variabilty Studies in Neolamarkia cadamba (Roxb.) Bosser Provenances at Nursery Level

2017 ◽  
Vol 40 (1) ◽  
pp. 33-40
Author(s):  
Makrand Gujar ◽  
◽  
Jyothi K. Mandan ◽  
A. Vijayraghavana ◽  
K.T. Parthiban ◽  
...  
Keyword(s):  
Biomass Production ◽  
Root Length ◽  
Quality Index ◽  
Growth Parameters ◽  
Field Trials ◽  
Shoot Length ◽  
Raw Material ◽  
Volume Index ◽  
Fast Growing ◽  
Collar Diameter

Neolamarkia cadamba (Roxb.) is a fast growing species recognized as a raw material for match wood industry, pulp wood, plywood industry. Seeds from thirty CPTs of Neolamarkia cadamba across the India were collected and evaluated in nursery for various growth and variability parameters. Significant variation was recorded for shoot length, root length, collar diameter, root shoot ratio, biomass production, sturdiness quotient, volume index and quality index among 30 genotypes. FCRIAC 6 (Pantnagar), have outperformed in seven growth parameters studied, followed by FCRIAC 11 (Chandrapur), FCRIAC 19 (Ragihosalli), FCRIAC 20 (Kodigehalli), FCRIAC 26 (Nashik) performing better in six growth parameters. Shoot length, biomass production and volume index registered high genetic estimates. Heritability was high for all the growth characters. PCV values were slightly higher for all the parameters compared to GCV. Collar diameter, shoot length and root length positively and significantly correlated with biomass, volume index, sturdiness quotient and quality index both genotypically and phenotypically. These results will assist in early selection of good performing and fast growing genotype for raising plantations as well as during further investigation in field trials.

Influence of Seed Inoculation Treatments on Yield and Quality of Green Gram (Vigna radiata L.)

2020 ◽  
Author(s):  
Priyanka Sharma ◽  
Prakash Borah
Keyword(s):  
Bacillus Megaterium ◽  
Trichoderma Harzianum ◽  
Root Length ◽  
Trichoderma Viride ◽  
Dry Weight ◽  
Shoot Length ◽  
Green Gram ◽  
Seed Inoculation ◽  
Stover Yield ◽  
Field Emergence

To study the effect of seed inoculation treatments with biological agents on seed germination, field emergence and its effect on seed during summer season. Seeds of green gram variety Pratap (SG-1) were treated with microbial formulations of seven treatments which consisted of-Rhizobium (T1), Trichoderma harzianum (T2), Trichoderma viride (T3), Bacillus megaterium (T4), Trichoderma harzianum + Trichoderma viride + Bacillus megaterium (T5), Rhizobium + Trichoderma harzianum + Trichoderma viride + Bacillus megaterium (T6) and Control (T7). Shoot length, root length and seedling dry weight were recorded after 20 days of sowing, observations on nodulation, yield and yield contributing characters were recorded at maturity and germination characteristics of the seeds were studied in the laboratory. It was observed that seeds treated with combined inoculation of Rhizobium @ 4 g + Bacillus megaterium @ 5ml/1000 ml of water + Trichoderma harzianum @ 5ml/1000 ml of water + Trichoderma viride @ 5ml/1000 ml of water (T6) recorded significantly higher field emergence (91.25%), speed of emergence (42.14), seedling dry weight (1.67mg), shoot length (25.48 cm), seed yield (992 kg ha-1), Stover yield (1870 kg ha-1), number of pods plant-1 (37), number of seeds pod-1 (13.25), 100 seed weight (3.63g) root length (9.22 cm) and nodulation (15).

scholarly journals Effect of osmopriming on germination of rice seed

2016 ◽  
Vol 41 (3) ◽  
pp. 451-460 ◽  
Author(s):  
MN Hasan ◽  
MA Salam ◽  
MMI Chowdhury ◽  
M Sultana ◽  
N Islam
Keyword(s):  
Root Length ◽  
Seed Quality ◽  
Growth Parameters ◽  
Field Capacity ◽  
Dry Mass ◽  
Germination Percentage ◽  
Shoot Length ◽  
Rice Seed ◽  
Germination Time ◽  
Mean Germination Time

The experiment was conducted at the Seed Laboratory, Department of Agronomy, Bangladesh Agricultural University, Mymensingh during the period from September to October, 2011 to study the effect of chemical priming of seed on germination and growth of rice seedling cv. BRRI dhan 29. Seeds were soaked in 3% and 5% solutions of CaCl2, ZnSO4 and KCl for 24, 30, 36, 42, 48, 54, 60 hours, respectively. A control was maintained where seeds were subjected to no priming treatment. The experiment was laid out in a Completely Randomized Design with three replications. Primed seeds were tested for germination and vigour on sandy soil media (60:40) in petridish under field capacity. Seed quality tests included mean germination time, shoot length, shoot dry mass, root length and root dry mass. The results revealed that priming treatments had significant influence on germination and all the growth parameters of rice seedlings. Priming with 3% ZnSO4 for 30 hours showed the highest germination percentage and the lowest mean germinaion time. Priming with 5% KCl for 54 hours showed the highest root length while 5% of the same solution for 24 hours showed the highest root dry mass. On the contrary, seeds having no priming treatment showed the lowest values for germination, vigour index, shoot length,shoot dry mass, root length and root dry mass and the highest mean germination time. The present study concludes that rice seed cv. BRRI dhan29 could be primed with 3% ZnSO4 solution for 30 hours for improving germination and seedling growth.Bangladesh J. Agril. Res. 41(3): 451-460, September 2016

scholarly journals Selenyum Uygulaması ile Yetiştirilen Adaçayı Bitkisinden Elde Edilen Hidrosollerin Çemen ve Kişniş Tohumlarının Çimlenmesi Üzerine Etkisi

2019 ◽  
Vol 7 (sp2) ◽  
pp. 36
Author(s):  
Ferit Özen ◽  
Gülsüm Yaldız ◽  
Mahmut Çamlıca ◽  
Halit Aşkın ◽  
Abdurrahman Başol
Keyword(s):  
Root Length ◽  
Germination Rate ◽  
Dry Weight ◽  
Shoot Length ◽  
Fresh Weight ◽  
Fenugreek Seeds ◽  
Mean Values ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
The Mean

This research was carried out in the climate chamber in order to determine the effect of hydrosols obtained from sage grown with selenium application on germination of fenugreek and coriander seeds. Different concentrations of sage hydrosol (2.5, 5, 7.5 and 10 ml) and purified water used as control were applied to the fenugreek and coriander seeds. Germination rate, fresh weight, dry weight, root length, shoot length and root / shoot ratio of fenugreek and coriander seeds were applied. Variance analysis was performed according to the split plot design and the mean values were compared with LSD test. According to the results of the research; germination rate of fenugreek seeds 38.35-51.00%, fresh weight 0.04-0.12 g, dry weight 0.001-0.035 g, root length 0.71-2.02 cm, shoot length 1.27-3.20 cm, root/shoot ratio varied between 0.34-0.82, germination rate of coriander seeds 18.61-39.21%, fresh weight 0.0011-0.034 g, dry weight 0.004-0.030 g, root length 1.5-4.58 cm, shoot length 1.08-3.69 cm, root/shoot ratio 0.47-2.51 were found. The highest germination rate was obtained in 2.5 ml dose of sage hydrosol (51.00%) in fenugreek seeds and the highest germination rate was found in 7.5 ml dose of sage hydrosol (39.21%) in coriander seeds.

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