Influence of Seed Inoculation Treatments on Yield and Quality of Green Gram (Vigna radiata L.)

Author(s):  
Priyanka Sharma ◽  
Prakash Borah

To study the effect of seed inoculation treatments with biological agents on seed germination, field emergence and its effect on seed during summer season. Seeds of green gram variety Pratap (SG-1) were treated with microbial formulations of seven treatments which consisted of-Rhizobium (T1), Trichoderma harzianum (T2), Trichoderma viride (T3), Bacillus megaterium (T4), Trichoderma harzianum + Trichoderma viride + Bacillus megaterium (T5), Rhizobium + Trichoderma harzianum + Trichoderma viride + Bacillus megaterium (T6) and Control (T7). Shoot length, root length and seedling dry weight were recorded after 20 days of sowing, observations on nodulation, yield and yield contributing characters were recorded at maturity and germination characteristics of the seeds were studied in the laboratory. It was observed that seeds treated with combined inoculation of Rhizobium @ 4 g + Bacillus megaterium @ 5ml/1000 ml of water + Trichoderma harzianum @ 5ml/1000 ml of water + Trichoderma viride @ 5ml/1000 ml of water (T6) recorded significantly higher field emergence (91.25%), speed of emergence (42.14), seedling dry weight (1.67mg), shoot length (25.48 cm), seed yield (992 kg ha-1), Stover yield (1870 kg ha-1), number of pods plant-1 (37), number of seeds pod-1 (13.25), 100 seed weight (3.63g) root length (9.22 cm) and nodulation (15).

2017 ◽  
Vol 9 (3) ◽  
pp. 1740-1743
Author(s):  
S. P. Monalisa ◽  
J. K. Beura ◽  
R. K. Tarai ◽  
M. Naik

An experiment on seed quality enhancement of common bean (Phaseolus vulgaris L.) var. S 9 (local) was conducted at the department of seed science and technology, OUAT, Bhubaneswar during 2013-14 by use of three biocontrol agents viz. Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescence. Seeds were bi-oprimed with the biocontrol agents at 40, 50 and 60 % concentration for 4,8,12 and 16 hours of soaking. Seeds were also hydro primed for 4,8,12 and 16 hours. Unprimed dry seed resulted in germination (69 %), shoot length (27.5 cm), root length (14 cm), seedling dry weight (1.71g), SVI-I (2859.2), SVI-II (118.0) and speed of germination (5.8) while hydro primed seeds resulted in germination (72%), shoot length (31.9 cm), root length (15 cm), seedling dry weight (1.80 g), SVI-1 (3375.9) SVI-II (129.8) and speed of germination (6.7). Trichoderma harzianum at 40% con-centration and for 4 hours of soaking resulted enhancement of above quality parameter like 13.0 % in germination, 21.1 % in shoot length, 20.7 % in root length, 31.6 % in seedling dry weight, 36 % in seedling vigour index-I, 48.1 % in seedling vigour index-II and 58.6 % in speed of germination over unprimed seeds. Bio priming with P. fluorescence ( at 40% concentration and for 4 hour) closely followed and at par with best treatment with 11.6 %, 18.2 %, 16.4 %, 30.4 %, 30.7 % and 56.9 % enhancement of above mentioned quality parameters, respectively.


Author(s):  
M. , C. Muthu ◽  
Amlan Sushree ◽  
Rajkamal Srivastava

A field and laboratory experiment was conducted at Department of Seed Science and Technology, GKVK, UAS, Bangalore during 2014 to assess the response of fertilizer levels and spacing on seed quality parameters of green gram (Vigna radiata) cv. KKM-3. Experimental results revealed that fertilizer application of 50 :100 :100 NPK kg ha-1 + Green gram rhizobium (200gm) + PSB-Bacillus megaterium (200gm) recorded highest seed density (1.69g/cc), Test weight (46.2g), EC (0.750 dsm-1) TDH activity (0.999), germination percent age (97.25%), shoot length (21.98cm), rootlength (18.61cm), seedling dry weight (252g), seedlingvigour index I and II (3947 and 2450), protein percent (23.8%) and fieldemergence (90%) superior over other fertilizer treatments.Among the planting geometry 60×10cm recorded highest germination percentage (96.12%) as compared to 45×10cm (94.77%) and 30×10cm (93.82%). Interaction of fertilizerlevels and spacingthrough the application of 50 :100 :100 NPK kg ha-1 + green gram rhizobium (200gm) + PSB-Bacillus megaterium (200gm) withplantinggeometry 60×10cm recorded higher seed quality parameters viz Test weight (48.3 g), protein per cent (24.5 %), germination percentage (98%), EC (0.73dsm-1), TDH activity (1.001), seedling dry weight (265 mg), shoot length (23.65 cm), root length (19.36 cm) and seedling vigour index I and II (4224 and 2603) and field emergence( 93%) were observed.


2017 ◽  
Vol 9 (1) ◽  
pp. 582-586
Author(s):  
R. J. Patel ◽  
T. R. Ahlawat ◽  
A. I. Patel ◽  
J. J. Amarcholi ◽  
B. B. Patel ◽  
...  

An experiment was carried out at Navsari Agricultural University, Navsari during 2014 to evaluate the effect of pre-sowing treatments on survival percentage and growth of mango rootstocks. Mango stones were soaked in aqueous solutions of GA3 (100 and 200 ppm), Beejamruth (2 % and 3 %) and Thiourea (1 % and 2 %) for 12 and 24 hours. The trial was evaluated in Completely Randomized Design based on factorial concept and the treatments were replicated thrice. Imposition of treatments led to significant differences at 5 % level of significance for all parameters chosen in this study. Mango stones when treated with Thiourea at 1 % had the maximum shoot length (49.93 cm), root length (34.38 cm), shoot dry weight (21.08 g) and total dry weight (26.36 g). The highest number of lateral roots (10.90) and survival percentage (64.17) was observed in mango stones dipped in 100 ppm GA3. Between the two soaking duration, soaking mango stones for 24 hours recorded higher values for shoot length (45.03 cm), root length (32.79 cm), number of lateral roots (9.83), survival percentage (62.72), shoot root fresh weight ratio (4.30), shoot dry weight (21.33 g), total dry weight (26.28 g) and shoot root dry weight ratio (4.32). Thus, survival percentage and growth of mango rootstocks can be substantially improved by soaking mango stones in aqueous solutions of 100 ppm GA3 or Thiourea at 1 % for 24 hours before sowing.


2016 ◽  
Vol 4 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Zaid Chachar ◽  
N. A. Chachar ◽  
Q.I. Chachar ◽  
S.M Mujtaba ◽  
G.A Chachar ◽  
...  

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length  (7.23 cm), root length  (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt.  (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas  MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.


2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.


Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.


2010 ◽  
Vol 56 (No. 4) ◽  
pp. 194-199 ◽  
Author(s):  
M. Kabir ◽  
M.Z. Iqbal ◽  
M. Shafiq ◽  
Z.R. Farooqi

The effects of lead on root, shoot and seedling length, leaf area, number of leaves, plant circumference, seedling dry weight, root/shoot and leaf area ratios of <I>Thespesia populnea</I> L. were determined in greenhouse under natural environmental conditions with and without phytotoxic metal ions at 5, 10, 15, 20, and 25 µmol/l. Lead treatments have a strong influence on the growth and development of <I>T</I>. <I>populnea</I> by reducing significantly (<I>P</I> &lt; 0.05) all the above parameters. Lead treatment at 5–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on seedling and root length, plant circumference and seedling dry weight of <I>T</I>.<I> populnea,</I> while lead treatment at 10–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on shoot length, number of leaves and leaf area as compared to control. Tolerance in <I>T</I>.<I> populnea</I> seedling at 25 µmol/l of lead treatment was lowest as compared to all other treatments.


2013 ◽  
Vol 1 (4) ◽  
pp. 228-232 ◽  
Author(s):  
P. K. Roy ◽  
M. A. R. Sarkar ◽  
S. K. Paul ◽  
A. Dey

An experiment was conducted at the Seed Laboratory of the Department of Agronomy, Bangladesh Agricultural University, Mymensingh during the period from January to April 2012 to study the effect of osmopriming of Boro rice seed (cv. BRRI dhan29). Seeds were soaked in 1%, 3% and 5% solutions of ZnSO4, KCl and CaCl2 for 24 hours, respectively, with 30 hours incubation at 35°C temperature. Seed quality tests such as percent germination, mean germination time, vigor index, shoot length, root length, shoot dry weight and root dry weight of rice seedlings were measured for all treatments. Population m-2 was also calculated to investigate the field establishment of primed seeds. The highest germination, vigor index, population m-2, shoot length and shoot dry weight at 15 and 30 DAS, root length at 15 DAS, root dry weight at 15 and 30 DAS and the lowest mean germination time was observed from priming of seeds with 3% ZnSO4. Exceptionally, priming with 3% CaCl2 showed the highest root length at 30 DAS. On the contrary, 3% and 5% CaCl2 priming treatment showed the lowest germination, vigor index, population m-2, shoot length and shoot dry weight at 15 and 30 DAS, root length at 15 DAS, root dry weight at 15 and 30 DAS and the highest mean germination time. It can be concluded that rice seed (cv. BRRI dhan29) can be primed with 3% ZnSO4 solution for improving germination and field establishment of rice seedlings.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9131  Int J Appl Sci Biotechnol, Vol. 1(4): 228-232


Author(s):  
Sabita Ghimire ◽  
Hariom Yadav ◽  
Prabesh Acharya ◽  
Raju Kharel

This study aimed to determine the effect of seed priming (on-farm, hydro, halo, osmo, vitamin c priming, and hardening) on the germination and seedling growth of spring rice varieties: hardinath-3, hardinath-1, and chaite-5 at ambient room temperature. A factorial completely randomized design (CRD) was carried out with two factors: methods of priming and varieties at agriculture knowledge center (AKC), kapilbastu during march 2020. There were, in total, 21 treatment combinations and four replications. Parameters observed were germination percentage, speed of germination, vigor index, root length, shoot length, fresh weight, and dry weight of rice seedlings. Hardinath-3 had superior performance in terms of germination percentage (95.35 %), vigor index (183.86), root length (11.51 cm) and shoot length (7.39 cm) compared to hardinath-1 and chaite-5; the speed of germination, however, was greater in hardinath-1. Seed hardening - alternate soaking (tap water for 24 hours) and drying - induced higher germination percentage (94.91 %), speed of germination (96.00), and vigor index (197.43) than did other priming methods; germination percentage of vitamin c primed seeds (94.75 %), nevertheless, were at statistical par with hardened seeds. Hardened hardinath-3 seeds were better in regards to germination percentage (97.50 %) and root length (12.92 cm), while the speed of germination (97.50) and vigor index (210.50) was greater in hardened hardinath-1. Vitamin c primed seeds of hardinath-3 and hardinath-1 were finer with dry weight, shoot length, and vigor index. In conclusion, farmers' are suggested to hardened seeds of hardinath-3 before sowing to promote uniform germination and growth.


HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 899D-899
Author(s):  
Moo R. Huh ◽  
Beyoung H. Kwack ◽  
Leonard P. Perry

Shoot length, leaf length and width, root length, and crown diameter were affected by 0.0-, 0.5-, 1.0-, and 5.0-mg·liter–1 uniconazole drench and 25- or 50-mg·liter–1 GA3 spray but not significantly by 0.0%, 2.5%, and 5.0% NaCl. Leaf width of H. syriacus Sieb. & Zucc. was not affected and that of H. syriacus L. significantly decreased, as NaCl concentration increased. Effect of NaCl on H. syriacus leaf width was offset by treatment with uniconazole but not by GA3 treatment. With 2.5% NaCl, dry weight of H. hamabo treated with uniconazole or GA3 increased and that of treated H. syriacus decreased. With 5.0% NaCl, dry weight of both species decreased with uniconazole or GA3. Calcium at 13.35 or 133.5 mM decreased the reduction of dry weight by NaCl treatment. The dry: fresh weight ratio of H. hamabo and H. syriacus treated with NaCl plus uniconazole was higher than that only treated with NaCl. GA3 treatment with NaCl did not affect the dry: fresh weight ratio for either species


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