Solid-phase structures of cerebrospinal fluid in diagnosis of early asymptomatic neurosyphilis

2018 ◽  
pp. 56-61
Author(s):  
С.Н. Шатохина ◽  
Н.А. Кузнецова ◽  
В.Н. Шабалин
Keyword(s):  
Cerebrospinal Fluid ◽  
Visual Analysis ◽  
Solid Phase ◽  
Biological Fluids ◽  
Laboratory Methods ◽  
Phase Structures ◽  
Morphological Picture ◽  
Diagnostic Technology

Цель проведённого исследования состояла в оценке эффективности визуального анализа твёрдофазных структур спинномозговой жидкости для диагностики ранних форм нейросифилиса. Методы. Использован метод краевой дегидратации биологических жидкостей, входящий в состав авторской диагностической технологии «Литос-система». Диагностика раннего асимптомного нейросифилиса заключается в выявлении деструктивных образований в форме овалов в морфологической картине твёрдой фазы спинномозговой жидкости. Результаты. Проведён сравнительный анализ результатов исследования спинномозговой жидкости у 19 больных с подтверждённым диагнозом «ранний асимптомный нейросифилис», полученных традиционными лабораторными методами и методом краевой дегидратации. Выявлено, что локализация овалов внутри сферолитов указывает на длительность заболевания нейросифилисом менее трёх лет, а вне сферолитов - от трех до пяти лет. Заключение. Метод краевой дегидратации позволяет диагностировать ранний асимптомный нейросифилис по наличию деструктивных образований в форме овалов в морфологической картине твёрдой фазы спинномозговой жидкости. The aim of this study was to evaluate effectiveness of visual analysis of solid-phase structures in cerebrospinal fluid to diagnose early forms of neurosyphilis. Methods. We used a method of marginal dehydration of biological fluids as a part of the author’s diagnostic technology, Litos-System. Early asymptomatic neurosyphilis is diagnosed based on detection of destructive, oval-shaped formations in the morphological picture of cerebrospinal fluid solid phase. Results. Data from analyses of cerebrospinal fluid performed with traditional laboratory methods and the method of marginal dehydration were compared for 19 patients with documented diagnosis of early asymptomatic neurosyphilis. A localization of ovals within spherulites indicated a less than a three-year duration of neurosyphilis while a localization outside spherulites indicated a duration of three to five years. Conclusion. The method of marginal dehydration allows detecting early asymptomatic neurosyphilis based on the presence of destructive, oval-shaped formations in the morphological picture of cerebrospinal fluid solid phase.

New ideas about the course of nephrolithiasis

2018 ◽  
pp. 273-276
Author(s):  
С.Н. Шатохина ◽  
Д.С. Уварова ◽  
В.Н. Шабалин
Keyword(s):  
Visual Analysis ◽  
Solid Phase ◽  
Chemical Elements ◽  
Biological Fluids ◽  
Stone Formation ◽  
Diagnostic Value ◽  
Urinary Stone ◽  
System Technology ◽  
Clinical Value ◽  
Phase Structures

Актуальность. Среди урологических заболеваний одним из ведущих является нефролитиаз. Существует несколько теорий его возникновения, однако ряд аспектов, касающихся вероятных причин его возникновения и механизмов развития, остаются актуальными для исследования и в настоящее время. Цель: представить динамику нефролитиаза по структурам твёрдой фазы мочи с интерпретацией диагностического значения основных морфологических образований. Методы. Формирование твёрдофазных структур мочи осуществлялось методом клиновидной дегидратации (технология «Литос-Система»). Количественное распределение химических элементов в различных зонах фаций мочи проводилось методом рентгеноспектрального микроанализа. Результаты. Технология исследования твёрдофазных структур мочи позволяет диагностировать процесс камнеобразования в почках, определять степень его активности и состав камнеобразующих солей мочи. Показано, что активность нефролитиаза определяется степенью сродства (прочностью связей) камнеобразующих солей с молекулами белка. Заключение. Изучение структур твёрдой фазы мочи у больных нефролитиазом позволило получить качественно новую диагностическую информацию о взаимодействии двух основных составляющих мочи - минеральной и органической. Механизм возникновения и развития нефролитиаза объясняется формированием аномально прочных молекулярных взаимосвязей между органической и минеральной составляющей мочи, что создаёт условия для появления патологических органо-минеральных агрегатов. Метод клиновидной дегидратации биологических жидкостей позволяет обнаружить эти агрегации в виде макроструктур, доступных для визуального анализа. Основное клиническое значение технологии «Литос-Система» состоит в её возможности чёткого контроля за течением нефролитиаза и формированием наиболее эффективных терапевтических программ. Aim. Among urological diseases a leading one is nephrolithiasis. There are several theories of its occurrence; however, studying many aspects of probable causalities and developmental mechanisms is still relevant. Objective: to determine the dynamics of nephrolithiasis by structure of urine solid phase and to interpret the diagnostic value of major morphological formations. Methods. Urine solid phase structures were formed using the method of cuneiform dehydration (Lithos-System technology). Quantitative distribution of chemical elements in different urine facie zones was determined using the X-ray microanalysis. Results. The technology of studying urine solid-phase structures allows to detect the process of stone formation in the kidneys and to detemine the process intensity and the composition of urinary stone-forming salts. It was shown that nephrolithiasis activity was determined by the degree of affinity (bond strength) of stone-forming salts to protein molecules. Conclusion. Studying structures of the urine solid phase in patients with nephrolithiasis provided qualitatively new diagnostic information about the interaction between two main components of urine, the mineral and organic ones. The mechanism for nephrolithiasis onset and development can be attributed to formation of abnormally strong molecular relationships between organic and mineral components, which creates conditions for emergence of pathological organo-mineral aggregates. The method of cuneiform dehydration of biological fluids allows detecting these aggregates in the form of macrostructures available for visual analysis. The main clinical value of the Litos-System technology is its ability to accurately monitor the course of nephrolithiasis and to support development of most effective therapeutic programs.

scholarly journals Razvoj SPE-HPLC-DAD metode za određivanje florfenikola i florfenikol amina u cerebrospinalnoj tekućini svinja

2020 ◽  
Vol 51 (2) ◽  
pp. 129-138
Author(s):  
Ksenija Šandor ◽  
Svjetlana Terzić ◽  
Anja Vujnović ◽  
Eleonora Perak Junaković ◽  
Irena Žarković ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
High Performance ◽  
Solid Phase ◽  
Biological Fluids ◽  
Experimental Period ◽  
Intramuscular Administration ◽  
Array Detector ◽  
Control Group ◽  
Pharmacokinetic Studies ◽  
Experimental Conditions

A study of florfenicol (FF) and its metabo- lite florfenicol amine (FFA) in pig cerebrospinal fluid was conducted following repeated intramuscular administration of the original (reference) and a generic veterinary medicinal product (VMP) under the same experimental conditions (20 mg FF/kg body weight, 48-hour interval). Both VMPs are solutions for injection containing FF as an active substance in the concentration of 300 mg/mL and have been authorized in Croatia for use in cattle and pigs. In this study, clinically healthy pigs were randomly divided into three groups. The first group was treated with the reference VMP, the second with the generic VMP, while the third served as the control group. Animals were sacrificed at 216, 288 and 384 hours after the first drug administration. Cerebrospinal fluid samples were analysed by the optimized and validated high-performance liquid chromatography-diode array detector method (HPLC-DAD). The solid-phase extraction (SPE) technique was chosen for sample preparation. The HPLC-DAD method provides good linearity over the concentration range of 0.05 to 5.00 μg/mL for FF and FFA. Limits of detection were 0.0023 μg/mL for FF and 0.0100 μg/mL for FFA. Extraction recoveries of FF were from 86.6% to 111.8%, and of FFA from 91.7% to 98.8%. The SPE-HPLC-DAD method has been demonstrated to be a selective, sensitive and suitable analytical method for the determination of FF and FFA in cerebrospinal fluid. The present study was based on a preliminary study that quantified FF in pig plasma at 216 hours after the first application of reference or generic VMP. However, FF and FFA were not detected in any of the cerebrospinal fluid samples during the experimental period. According to the nature of biological fluids, the SPE-HPLC-DAD method can be suitable for further pharmacokinetic studies of FF in pig plasma and serum after intramuscular administration of VMPs.

History of formation and development of laboratory diagnostics

2020 ◽  
pp. 10-14
Author(s):  
Sergey Borisovich Panchenko
Keyword(s):  
Cerebrospinal Fluid ◽  
Visual Analysis ◽  
Clinical Laboratory ◽  
Biological Fluids ◽  
Clinical Decision ◽  
Ancient Egypt ◽  
Laboratory Research ◽  
Laboratory Diagnostics ◽  
Medical Diagnostic ◽  
History Of

Clinical laboratory diagnostics is a medical diagnostic specialty that provides a set of studies of the biomaterial of the human body with the aim of further comparing the results with the data of a clinical examination and establishing a diagnosis. Thanks to laboratory research, practical healthcare has the opportunity to receive about 70% of the volume of objective diagnostic information necessary for the timely adoption of the correct clinical decision, the appointment of appropriate treatment, and subsequently ensuring control over the effectiveness of its implementation. The object of research in laboratory diagnostics is an individual, and the subject of research is the biomaterial obtained from him (saliva, urine, semen, blood, serum, cerebrospinal fluid, exudate, etc.) [3]. The history of the development of laboratory diagnostics has more than one hundred years. The earliest information that has come down to us, which can be considered a prototype of laboratory diagnostics, refers to Ancient Egypt. In the “Surgical Papyrus”, the information was found on the possible conduct of a visual analysis of biological fluids - this paper provides a description of the state of cerebrospinal fluid in injured patients. At the same time, the first mentions of parasitology appeared — there is an assumption that Muslims do not eat pork, as many years ago they discovered the presence of parasites in it. The work “Forecast”, which belongs to the pen of Hippocrates, contains a rather detailed description of the properties and macroscopic characteristics of urine, feces and sputum in normal conditions and in various diseases. However, the emergence of laboratory diagnostics as an independent branch of medicine occurred much later, at the turn of the 19th and 20th centuries.

Marker structures in the liquor of patients with destructive processes in brain tissues

2020 ◽  
pp. 1-4
Keyword(s):  
Liquid Phase ◽  
Bacterial Meningitis ◽  
Solid Phase ◽  
Malignant Tumors ◽  
Biological Fluids ◽  
Brain Diseases ◽  
Phase Structures ◽  
Destructive Processes ◽  
Brain Tissues

The paper presents the results of studies the solid phase structures of liquor 140 patients’ with various types of destructive brain diseases (74 - neurosyphilis, 26 - bacterial meningitis, 7 - aneurysms, 9 - malignant tumors, 24 - discogenic radiculitis). The transfer of liquor into the solid phase was carried out by the method of marginal dehydration of biological fluids. This method allows to visualize the structure of metabolites of the liquid phase of liquor. The results of the study showed presence in the patients’ liquor marker structures, which could be used to clarify the diagnosis.

scholarly journals Metabolic Evaluation of Urine from Patients Diagnosed with High Grade (HG) Bladder Cancer by SPME-LC-MS Method

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2194
Author(s):  
Kamil Łuczykowski ◽  
Natalia Warmuzińska ◽  
Sylwia Operacz ◽  
Iga Stryjak ◽  
Joanna Bogusiewicz ◽  
...  
Keyword(s):  
Thin Film ◽  
Bladder Cancer ◽  
High Performance ◽  
Biomarker Discovery ◽  
Solid Phase ◽  
Biological Fluids ◽  
Reversed Phase ◽  
Control Group ◽  
Metabolic Evaluation ◽  
Metabolomic Profiling

Bladder cancer (BC) is a common malignancy of the urinary system and a leading cause of death worldwide. In this work, untargeted metabolomic profiling of biological fluids is presented as a non-invasive tool for bladder cancer biomarker discovery as a first step towards developing superior methods for detection, treatment, and prevention well as to further our current understanding of this disease. In this study, urine samples from 24 healthy volunteers and 24 BC patients were subjected to metabolomic profiling using high throughput solid-phase microextraction (SPME) in thin-film format and reversed-phase high-performance liquid chromatography coupled with a Q Exactive Focus Orbitrap mass spectrometer. The chemometric analysis enabled the selection of metabolites contributing to the observed separation of BC patients from the control group. Relevant differences were demonstrated for phenylalanine metabolism compounds, i.e., benzoic acid, hippuric acid, and 4-hydroxycinnamic acid. Furthermore, compounds involved in the metabolism of histidine, beta-alanine, and glycerophospholipids were also identified. Thin-film SPME can be efficiently used as an alternative approach to other traditional urine sample preparation methods, demonstrating the SPME technique as a simple and efficient tool for urinary metabolomics research. Moreover, this study’s results may support a better understanding of bladder cancer development and progression mechanisms.

scholarly journals Algorithm for detecting spherulite marker in human blood serum facies

2019 ◽  
pp. 109-113
Author(s):  
O E Malenova ◽  
L I Trubnikova ◽  
A S Yashina ◽  
M L Albutova
Keyword(s):  
Thin Film ◽  
Mammary Gland ◽  
Medical Diagnosis ◽  
Visual Analysis ◽  
Biological Fluids ◽  
Uterine Fibroids ◽  
Detection Methods ◽  
Atypical Form ◽  
Probable Presence ◽  
Characteristic Features

One of the effective methods of early medical diagnosis is the method of wedge dehydration. It is based on the analysis of facies images. Facia is a thin film of dried human biological fluids. The presence of special structures (markers) indicates various pathologies of the organism at their earliest stages. In this article, the algorithm for detecting spherulite marker on microscopic images of human serum facies is presented. The presence of spherulites on facies is the norm. However, the atypical form of spherulite is a marker of precancerous diseases: uterine fibroids, endometrial hyperplastic processes and the mammary gland. Due to the visual analysis of the marker, its characteristic features were identified. Then algorithmic detection methods for these features were developed. The decision on the probable presence of a marker was made if there was a combination of features of this marker. As a result of the application of the developed algorithm, most images of atypical spherulites were identified.

MONITORING OF MALIGNANT GROWTH DYNAMICS BY STRUCTURAL ANALYSIS OF NON-CELLULAR TISSUE

2021 ◽  
pp. 89-96
Author(s):  
V.N. Shabalin ◽  
S.N. Shatokhina ◽  
M.G. Dedova
Keyword(s):  
Blood Serum ◽  
Laryngeal Cancer ◽  
Solid Phase ◽  
Biological Fluids ◽  
Treatment Options ◽  
Developmental Phase ◽  
Malignant Growth ◽  
Active Growth ◽  
Main Research ◽  
Degree Of Anisotropy

The authors examined the composition of biocrystalline structures (anisomorphones) of blood serum in patients with laryngeal cancer. Such structures are formed when blood serum becomes solid, i.e. during its marginal dehydration. The revealed anisomorphones represent three types of marker structures: a marker of a malignant tumor active growth (the aggregation of macroferolite and granular microspherolite with the same degree of anisotropy); a marker of a degenerative-dystrophic process (the aggregation of a macrospherolite with a low degree of anisotropy and microspherolite with a high degree of anisotropy); a marker of a malignant growth progression (a wavy microspherolite without aggregation). The aim of the study is to identify diagnostic markers of the malignant process activity in the solid phase structures of the blood serum in patients with laryngeal cancer and to assess their importance for choosing an effective therapy. Materials and Methods. Marginal dehydration of blood serum was used as the main research method. It is a part of the "Litos-system" diagnostic technology (Marketing authorization FS No. 155, of 2009). Results. It has been shown that the developmental phase of laryngeal cancer (active growth or degenerative-dystrophic process) is an important criterion for choosing treatment options. Surgical treatment is the most effective during the degenerative-dystrophic tumor process, while radiation therapy is preferable during the active phase of malignant growth. Key words: laryngeal cancer, blood serum, marginal dehydration of biological fluids, markers of tumor growth activity. Исследован состав биокристаллических структур (анизоморфонов) сыворотки крови больных раком гортани, которые формируются при переходе сыворотки крови в твердую фазу в процессе ее краевой дегидратации. Выявленные анизоморфоны представляют собой три вида маркерных структур: маркер активного роста злокачественной опухоли – агрегация макросферолита и зернистого микросферолита с одинаковой степенью анизотропии; маркер дегенеративно-дистрофического процесса – агрегация макросферолита с низкой степенью анизотропии и микросферолита с высокой степенью анизотропии; маркер прогрессии злокачественного роста – волнистый микросферолит вне агрегации. Цель – выявить диагностические маркеры активности злокачественного процесса в структурах твердой фазы сыворотки крови больных раком гортани и оценить их значение для выбора эффективного вида лечения. Материалы и методы. В качестве основного метода исследования использован метод краевой дегидратации сыворотки крови, являющийся разделом диагностической технологии «Литос-система» (Разрешение ФС № 155 от 2009 г. на применение в клинической практике). Результаты. Показано, что фаза развития рака гортани (активный рост или дегенеративно-дистрофический процесс) служит важным критерием выбора вида лечения: в фазу дегенеративно-дистрофического процесса опухоли наиболее благоприятный эффект дает хирургическое лечение, а в период активной фазы злокачественного роста – лучевая терапия. Ключевые слова: рак гортани, сыворотка крови, краевая дегидратация биологических жидкостей, маркеры фазы активности опухолевого роста.

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