Abrogation of Sox9 expression in the endothelium blocks aberrant vascular EndMT and fibrosis

2021 ◽  
Author(s):  
Jilai Zhao
Keyword(s):  
Sox9 Expression

scholarly journals RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3192
Author(s):  
Antoine Gleizes ◽  
Mouna Triki ◽  
Sandrine Bonnet ◽  
Naomi Baccari ◽  
Gabriel Jimenez-Dominguez ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Differentiation ◽  
Paneth Cell ◽  
Wnt Signaling Pathway ◽  
Cell Lineage ◽  
Human Colon ◽  
Luciferase Reporter ◽  
Loss Of Function ◽  
Sox9 Expression ◽  
Human Colon Cancer

RIP140 is a major transcriptional coregulator of gut homeostasis and tumorigenesis through the regulation of Wnt/APC signaling. Here, we investigated the effect of RIP140 on Paneth cell differentiation and its interplay with the transcription factor SOX9. Using loss of function mouse models, human colon cancer cells, and tumor microarray data sets we evaluated the role of RIP140 in SOX9 expression and activity using RT-qPCR, immunohistochemistry, luciferase reporter assays, and GST-pull down. We first evidence that RIP140 strongly represses the Paneth cell lineage in the intestinal epithelium cells by inhibiting Sox9 expression. We then demonstrate that RIP140 interacts with SOX9 and inhibits its transcriptional activity. Our results reveal that the Wnt signaling pathway exerts an opposite regulation on SOX9 and RIP140. Finally, the levels of expression of RIP140 and SOX9 exhibit a reverse response and prognosis value in human colorectal cancer biopsies. This work highlights an intimate transcriptional cross-talk between RIP140 and SOX9 in intestinal physiopathology.

scholarly journals Loss of SOX9 Expression Is Associated with PSA Recurrence in ERG-Positive and PTEN Deleted Prostate Cancers

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128525 ◽  
Author(s):  
Christoph Burdelski ◽  
Erzen Bujupi ◽  
Maria Christina Tsourlakis ◽  
Claudia Hube-Magg ◽  
Martina Kluth ◽  
...  
Keyword(s):  
Sox9 Expression ◽  
Psa Recurrence ◽  
Prostate Cancers

scholarly journals The Clinicopathological and Prognostic Significance of SOX9 Expression in Gastric Cancer: Meta-Analysis and TCGA Analysis

2021 ◽  
Vol 11 ◽  
Author(s):  
Guo Zu ◽  
Jiacheng Gao ◽  
Tingting Zhou
Keyword(s):  
Gastric Cancer ◽  
Meta Analysis ◽  
Prognostic Significance ◽  
Tnm Stage ◽  
The Cancer Genome Atlas ◽  
Depth Of Invasion ◽  
Sox9 Expression ◽  
Potential Prognostic Factor ◽  
Clinicopathological Significance ◽  
Tcga Dataset

BackgroundThe clinicopathological and prognostic significance of SRY-box transcription factor 9 (SOX9) expression in gastric cancer (GC) patients is still controversial. Our aim is to investigate the clinicopathological and prognostic value of SOX9 expression in GC patients.MethodsA systemic literature search and meta-analysis were used to evaluate the clinicopathological significance and overall survival (OS) of SOX9 expression in GC patients. The Cancer Genome Atlas (TCGA) dataset was used to investigate the relationship between SOX9 expression and OS of stomach adenocarcinoma (STAD) patients.ResultsA total of 11 articles involving 3,060 GC patients were included. In GC patients, the SOX9 expression was not associated with age [odds ratio (OR) = 0.743, 95% CI = 0.507–1.089, p = 0.128], sex (OR = 0.794, 95% CI = 0.605–1.042, p = 0.097), differentiation (OR = 0.728, 95% CI = 0.475–1.115, p = 0.144), and lymph node metastasis (OR = 1.031, 95% CI = 0.793–1.340, p = 0.820). SOX9 expression was associated with depth of invasion (OR = 0.348, 95% CI = 0.247–0.489, p = 0.000) and TNM stage (OR = 0.428, 95% CI = 0.308–0.595, p = 0.000). The 1-year OS (OR = 1.507, 95% CI = 1.167–1.945, p = 0.002), 3-year OS (OR = 1.482, 95% CI = 1.189–1.847, p = 0.000), and 5-year OS (OR = 1.487, 95% CI = 1.187–1.862, p = 0.001) were significantly shorter in GC patients with high SOX9 expression. TCGA analysis showed that SOX9 was upregulated in STAD patients compared with that in normal patients (p < 0.001), and the OS of STAD patients with a high expression of SOX9 is poorer than that in patients with low expression of SOX9, but the statistical difference is not obvious (p = 0.31).ConclusionSOX9 expression was associated with the depth of tumor invasion, TNM stage, and poor OS of GC patients. SOX9 may be a potential prognostic factor for GC patients but needs further study.Systematic Review RegistrationPROSPERO, ID NUMBER 275712.

scholarly journals Determination of SOX9 Gene Expression In Brain Tumours Among East Coast Malaysia Population

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Md Dzali NB ◽  
Wan Taib WR ◽  
Zahary MN ◽  
Abu Bakar NH ◽  
Abd Latif AZ ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Brain Tumour ◽  
Brain Tumours ◽  
East Coast ◽  
Rna Extraction ◽  
Slight Modification ◽  
Low Grade ◽  
Normal Brain ◽  
Sox9 Expression ◽  
Sox9 Gene

Introduction: SOX9, a members of SOX family, plays a significant roles in developmental processes during embryogenesis, including brain tissue. Few studies have shown that SOX9 has been involved in tumourigenesis of several types of cancer including brain tumour. However, such studies are still lacking in the Malaysian population. The aim of this study was to determine SOX9 expression level in several types of brain tumours in East Coast Malaysia. Materials and Methods: Five formalin-fixed pariffin-embedded brain tumour samples of Malay descendants were sectioned by using microtome. RNA extraction was performed with slight modification by adding Trizol during tissue lysis. The RNA was converted to cDNA using reverse transcription technique before SOX9 expression was detected using RT q-PCR assay in brain tumours normalized to non-neoplastic brain tissues. Results: Overall results displayed that SOX9 gene in all samples were up-regulated. SOX9 overexpression was found in both high and low grade glioma (anaplastic and pilocytic astrocytoma respectively). This is consistence with both low grade (benign) and atypical meningioma. Secondary brain tumour also showed up-regulation when compared to normal brain tissue. Conclusion: Up-regulation in SOX9 expression in selected brain tumours in Malay patients revealed its significant roles in brain tumourigenesis. Functional studies should be carried out to observe the SOX9 functions and mechanism whether they should reflect their diverse roles in Malaysia population.

scholarly journals Sox9 expression and ultrastructure of the endocardial cushions during the heart development in Xenopus laevís

2007 ◽  
Vol 31 (2) ◽  
pp. 81-89
Author(s):  
Won Seok Koh ◽  
Li Guang Li ◽  
In-Shik Kim ◽  
Young-Hoon Lee ◽  
Byung Seok Ko ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Heart Development ◽  
Endocardial Cushions ◽  
Sox9 Expression

Microbiota‐derived lipopolysaccharide retards chondrocyte hypertrophy in the growth plate through elevating Sox9 expression

2018 ◽  
Vol 234 (3) ◽  
pp. 2593-2605 ◽  
Author(s):  
Xin Cheng ◽  
Pei‐Zhi Li ◽  
Guang Wang ◽  
Yu Yan ◽  
Ke Li ◽  
...  
Keyword(s):  
Growth Plate ◽  
Sox9 Expression ◽  
Chondrocyte Hypertrophy

Hoxa-2 restricts the chondrogenic domain and inhibits bone formation during development of the branchial area

Development ◽  
1998 ◽  
Vol 125 (14) ◽  
pp. 2587-2597 ◽  
Author(s):  
B. Kanzler ◽  
S.J. Kuschert ◽  
Y.H. Liu ◽  
M. Mallo
Keyword(s):  
Bone Formation ◽  
Branchial Arch ◽  
Intramembranous Ossification ◽  
Expression Domain ◽  
Sox9 Expression ◽  
Molecular Analyses ◽  
Early Stages ◽  
Branchial Arches ◽  
Skeletal Formation ◽  
Dermal Bone

In Hoxa-2(−/−)embryos, the normal skeletal elements of the second branchial arch are replaced by a duplicated set of first arch elements. We show here that Hoxa-2 directs proper skeletal formation in the second arch by preventing chondrogenesis and intramembranous ossification. In normal embryos, Hoxa-2 is expressed throughout the second arch mesenchyme, but is excluded from the chondrogenic condensations. In the absence of Hoxa-2, chondrogenesis is activated ectopically within the rostral Hoxa-2 expression domain to form the mutant set of cartilages. In Hoxa-2(−/−)embryos the Sox9 expression domain is shifted into the normal Hoxa-2 domain. Misexpression of Sox9 in this area produces a phenotype resembling that of the Hoxa-2 mutants. These results indicate that Hoxa-2 acts at early stages of the chondrogenic pathway, upstream of Sox9 induction. We also show that Hoxa-2 inhibits dermal bone formation when misexpressed in its precursors. Furthermore, molecular analyses indicate that Cbfa1 is upregulated in the second branchial arches of Hoxa-2 mutant embryos suggesting that prevention of Cbfa1 induction might mediate Hoxa-2 inhibition of dermal bone formation during normal second arch development. The implications of these results on the patterning of the branchial area are discussed.

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