INFLUENCE OF YEAST RACE ON THE CONCENTRATION OF BIOGENIC AMINES IN WHITE TABLE WINE MATERIAL

2021 ◽  
Author(s):  
Н.М. АГЕЕВА ◽  
М.Г. МАРКОВСКИЙ ◽  
А.В. КЛОЧКО
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biogenic Amines ◽  
High Performance ◽  
Bacterial Cells ◽  
Grape Variety ◽  
Grape Must ◽  
Significant Difference ◽  
End Use ◽  
Grape Must Fermentation

Рассмотрено влияние расы дрожжей на концентрацию биогенных аминов в белом столовом виноматериале. Для эксперимента использовали стерильное сусло винограда сорта Алиготе, не содержавшее бактериальных клеток. Сусло сбраживали в стерильных условиях расами дрожжей российского производства и расами активных сухих дрожжей производства европейских фирм. Использованные расы дрожжей различались по целевому назначению. Брожение проводили в одинаковых для всех образцов условиях при температуре 18–20°С. По окончании брожения виноматериалы отделяли от дрожжевой гущи путем фильтрации и определяли концентрацию биогенных аминов методом высокоэффективной жидкостной хроматографии. Установлено существенное различие концентраций биогенных аминов в образцах белого столового виноматериала, изготовленных с использованием различных рас дрожжей: количество гистамина варьировало от 0,08 (Vinomax Yeast) до 4,26 мг/дм3 (Pro Elif); концентрация тирамина – от 0,36 (Oenoferm Rouge) до 2,21 мг/дм3 (ОК 2); содержание фенилэтиламина в образцах виноматериала составляло от 0,17 (Oenoferm Rouge) до 1,23 мг/дм3(Prise de Musso); концентрация путресцина – от 0,01 (Prima Vera) до 0,33 мг/дм3 (Виниферм). В образцах виноматериала, полученных с применением дрожжей рас Oenoferm Rouge, SP 49, Vinomax Yeast, кадаверин не обнаружен. Сбраживание сусла дрожжами рас Виниферм, L food I fantastici, Шампанская 7-10С, France Cuve, Prise de Musso привело к накоплению кадаверина в количестве 0,27 мг/дм3 и выше. Таким образом, доказано, что раса дрожжей, применяемая для сбраживания виноградного сусла, оказывает влияние на концентрацию биогенных аминов в виноматериале. The influence of the race of yeast on the concentration of biogenic amines in the white table materials was explored. The sterile must of a grape variety Aligote without bacterial cells was used for the experiment. The must was fermented in the sterile conditions using races of yeasts of Russian manufacture and races of the European produced active dry yeasts. Applied races of yeasts differ by the end use. Fermentation was carried out in the similar conditions for all samples at temperature 18–20°C. On completion of the fermentation wine materials were separated from the yeast sediment and the concentrations of biogenic amines were determined by a method of high-performance liquid chromatography. Significant difference in the biogenic amines concentrations has been established in the samples of white table materials developed with various races of yeasts: amount of histamine varied from 0,08 (Vinomax Yeast) to 4,26 mg/dm3 (Pro Elif); amount of tyramine varied from 0,36 (Oenoferm Rouge) to 2,21 mg/dm3 (OK 2); amount of phenylethylamine in the wine material varied from 0,17 (Oenoferm Rouge) to 1,23 mg/dm3(Prise de Musso); amount of putrescine varied from 0,01 (Oenoferm Rouge) to 0,33 mg/dm3(Viniferm). Cadaverine was not found in the wine samples obtained using races of yeasts Oenoferm Rouge, SP 49, Vinomax Yeast. Must fermentation using races of yeasts Viniferm, L food I fantastici, Shampanskaya 7-10C, France Cuve, Prise de Musso resulted in the cadaverine accumulation in amount greater than 0,27 mg/dm3. Therefore, it was proved that the race of yeast applied to the grape must fermentation makes an impact on the biogenic amines concentration in a wine material.

INFLUENCE OF WORT FERMENTATION CONDITIONS ON THE CONTENT OF BIOGENIC AMINES IN WHITE TABLE WINE MATERIALS

2021 ◽  
Author(s):  
Н.М. АГЕЕВА ◽  
А.А. ШИРШОВА ◽  
А.В. МАЦКУЛ
Keyword(s):  
Experimental Data ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biogenic Amines ◽  
High Performance ◽  
Grape Variety ◽  
Fermentation Conditions ◽  
Grape Must ◽  
Laboratory Conditions ◽  
Race Mixing

Изучено влияние условий и способа брожения виноградного сусла на концентрацию биогенных аминов в белых столовых виноматериалах. Виноматериалы приготовлены путем сбраживания сусла винограда сорта Шардоне расой дрожжей Pro Elif, обладающей наибольшей декарбоксилирующей способностью, и расой Судак VI-5 (контроль), обеспечившей наименьшее накопление биогенных аминов. Брожение сусла проводили в лабораторных условиях стационарным, непрерывным и дробно-доливным способами. Концентрации биогенных аминов в виноматериалах определены методом высокоэффективной жидкостной хроматографии. Представлены экспериментальные данные о содержании биогенных аминов – гистамина, метиламина, кадаверина, путресцина и фенилэтиламина в белых столовых виноматериалах, произведенных с применением различных способов брожения с перемешиванием бродящей среды и без него. Установлено, что существенное влияние на концентрацию биогенных аминов оказывает раса дрожжей. Применение расы Pro Elif привело к большему образованию биогенных аминов в сравнении с расой Судак VI-5. Перемешивание не оказало существенного влияния на количество биогенных аминов. Показано, что при дображивании увеличилась концентрация гистамина, метиламина и фенилэтиламина. Установлено, что наибольшее увеличение концентрации суммы биогенных аминов отмечается при выдержке виноматериала на дрожжевом осадке, особенно при отсутствии перемешивания. The influence of the conditions and method of fermentation of grape must on the concentration of biogenic amines in white table wine materials was studied. Wine materials prepared by fermenting the wort of the Chardonnay grape variety by the Pro Elif yeast race, which has the greatest decarboxylating ability, and the Sudak VI-5 race (control), which provided the least accumulation of biogenic amines. Fermentation of the wort was carried out under laboratory conditions in a stationary, continuous and fractional-topping way. The concentrations of biogenic amines in wine materials were determined by high-performance liquid chromatography. Experimental data on the content of biogenic amines – histamine, methylamine, cadaverine, putrescine and phenylethylamine in white table wine materials produced using various fermentation methods with and without stirring of the fermenting medium are presented. It was found that the yeast race has a significant effect on the concentration of biogenic amines. The use of the Pro Elif race led to a greater formation of biogenic amines in comparison with the Sudak VI-5 race. Mixing did not significantly affect the amount of biogenic amines. It was shown that the concentration of histamine, methylamine and phenylethylamine increased during fermentation. It was found that the greatest increase in the concentration of the sum of biogenic amines is observed when the wine material is aged on a yeast lees, especially in the absence of mixing.

scholarly journals Influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures

2014 ◽  
Vol 32 (1) ◽  
pp. 227-232 ◽  
Author(s):  
M.A.P. Oliveira ◽  
U.R. Antuniassi ◽  
E.D. Velini ◽  
R.B. Oliveira ◽  
J.F. Salvador ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mineral Oil ◽  
Mato Grosso ◽  
Significant Difference ◽  
Flight Height ◽  
Mixture Volume ◽  
Pasture Area ◽  
Experimental Plots

The objective of the present study was to analyze the influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures. The experimental plots were arranged in a pasture area in the district of Porto Esperidião (Mato Grosso, Brazil). In all of the treatments, the applications contained the herbicides aminopyralid and fluroxypyr (Dominum) at the dose of 2.5 L c.p. ha-1, including the adjuvant mineral oil (Joint Oil) at the dose of 1.0 L and a tracer to determine the deposition by high-performance liquid chromatography (HPLC) (rhodamine at a concentration of 0.6%). The experiment consisted of nine treatments that comprised the combinations of three spray volumes (20, 30 and 50 L ha-1) and three flight heights (10, 30 and 40 m). The results showed that, on average, there was a tendency for larger deposits for the smallest flight heights, with a significant difference between the heights of 10 and 40 m. There was no significant difference among the deposits obtained with the different spray mixture volumes.

scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

scholarly journals Determination of haemoglobin A1c levels using high-performance liquid chromatography of bloodstains

2019 ◽  
Vol 60 (1) ◽  
pp. 19-25
Author(s):  
Kemalettin Acar ◽  
Ayse Kurtulus Dereli ◽  
Esin Avci ◽  
Volkan Zeybek ◽  
Erdi Kutlu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Reliability ◽  
Receiver Operating Curve ◽  
Haemoglobin A1c ◽  
Receiver Operating Curve Analysis ◽  
Significant Difference ◽  
Glass Surfaces ◽  
Hba1c Levels

This study aimed to determine haemoglobin A1c (HbA1c) levels in bloodstains shed on glass and fabric surfaces on specified test dates. Blood samples were taken from 26 patients (13 diabetic and 13 non-diabetic). Initial HbA1c levels were detected by using high-performance liquid chromatography (HPLC), and bloodstains were created on both cotton fabric and glass surfaces. Samples were processed at different ages (0, 7, 14, 28 and 56 days) by diluting distilled water and then measuring HbA1c levels by HPLC again. In all stains, HbA1c levels could be determined by using HPLC, but there was a moderate rise in accordance with the age of the stains. A statistically significant difference was found for bloodstains on clothes compared to those on glass surfaces. Receiver operating curve analysis found a sensitivity of 1.0 and specificity of 0.923 (cut-off 6.55) for glass surfaces on the seventh day; a sensitivity of 1.0, a specificity of 0.846 (cut-off 6.45) for clothes on the seventh day; a sensitivity of 1.0 and a specificity of 0.923 (cut-off 6.85) for clothes on the 56th day; and a sensitivity of 1.0 and a specificity of 0.846 (cut-off 7.55) for glass surfaces on the 56th day. In conclusion, this study found that HbA1c levels could be measured with high reliability from forensic bloodstains by using HPLC. Thus, in cases where DNA data banks cannot identify individuals, it would make sense to turn to those who have a medical history of diabetes among the suspects with the results of high HbA1c levels.

scholarly journals Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

2016 ◽  
Vol 10 (01) ◽  
pp. 092-096 ◽  
Author(s):  
Huma Omurlu ◽  
Hacer Deniz Arisu ◽  
Evrim Eliguzeloglu Dalkilic ◽  
Ugur Tamer ◽  
Hilal Torul
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Bisphenol A ◽  
Root Canal ◽  
High Performance ◽  
Light Emitting Diode ◽  
Root Canal Sealer ◽  
Chromatography Analysis ◽  
Significant Difference ◽  
Time Periods

ABSTRACT Objective: The purpose of the current study was to determine the amount of urethane dimethacrylate (UDMA), bisphenol A-glycidyl methacrylate (Bis-GMA), poly (ethylene glycol) dimethacrylate (PEGDMA), bisphenol A ethoxylated dimethacrylate (Bis-EMA), and 2-hydroxyethyl methacrylate (HEMA) eluted from resin-based root canal sealer, epiphany, using high-performance liquid chromatography (HPLC). Materials and Methods: Epiphany was placed into the plastic molds and light-cured with a light emitting diode. After the curing process, each specimen in the first group (n = 12) was immersed in Eppendorf tubes containing a phosphate-buffered saline solution (PBS) and incubated for 45 s. In the second group, each specimen (n = 12) was immersed in Eppendorf tubes containing PBS and incubated for 24 h. Of the specimen extracts, 100 μL were subjected to HPLC. Analysis of data was accomplished with one-way analysis of variance (P < 0.05). Results: All of the samples eluted HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA. A significant difference was determined between the time periods of HEMA, UDMA, PEGDMA, and Bis-EMA (P < 0.05). Conclusion: The results of the current study showed that Epiphany releases HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA in both time periods.

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