Features of seminal plasma cytokine profile and an occurrence of pregnancy when carrying out a program of assisted reproductive technologies

GYNECOLOGY ◽  
2019 ◽  
Vol 21 (4) ◽  
pp. 9-13
Author(s):  
Tatiana V Dontsova ◽  
Alina A Babayan ◽  
Marina A Nikolaeva ◽  
Alla S Arefieva ◽  
Elena A Kalinina ◽  
...  
Keyword(s):  
Sexual Intercourse ◽  
Seminal Plasma ◽  
Transforming Growth Factor ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Female Reproductive Tract ◽  
Cytokine Profile ◽  
Sexual Life ◽  
Operating Characteristics ◽  
Diagnostic Significance

Aim. To asses an effectiveness of assisted reproductive technologies (ART) program given characteristics of the cytokine profile of seminal plasma (SP) entering the female reproductive tract during sexual intercourse. Outcomes and methods. 33 married couples who applied for a treatment of infertility by means of in vitro fertilization /ICSI (Intracytoplasmic Sperm Injection) were included in the prospective study. Patients were recommended to have sexual intercourse with no restrictions during treatment and to have the last sexual intercourse 3 days before an intended transvaginal puncture (ITP). Testing of cytokines (transforming growth factor (TGF)-b1, interferon (IFN)-g, interleukin (IL)-33, IL-6, IL-8, IL-23, IL-10, tumor necrosis factor (TNF)-a, IL-18, IL- 17A, IFN-a, IL-12, monocyte chemotactic protein-1) levels in samples of partners’ SP obtained on the day having ITP were carried out using multiplex analysis with LegendPlex kits (BioLegend, USA). Results. When comparing a cytokine profile of SP in couples who did not become pregnant (n=25) and couples who become pregnant (n=8) increased IL-18 and reduced IL-10 levels (p=0,017 and p=0, 01 respectively) were revealed in the group which got pregnant. To assess a clinical relevance of cytokine content in SP ROC (Receiver Operating Characteristics) curve was used. It was established that determining of IL-18 concentration in SP has the greatest diagnostic significance (the area under a curve was 0.792±0.107, test sensitivity - 62.5%, test specificity - 95.24% at threshold concentration>210.43 pg/ml). Incidence of pregnancy at IL-18 concentration above threshold levels was 83,3% while at lower concentrations of IL-18 pregnancy occurred only in 13.0% of women. Conclusions. Elevated IL-18 levels and decreased IL-10 levels in SP of female patients’ partners who don’t have restriction of sexual life when treating infertility with ART are favorable factors for a pregnancy to occur.

scholarly journals From One Ejaculate to Another: Transference of Sperm Traits via Seminal Plasma Supplementation in the Ram

Biology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 33
Author(s):  
Christine Green ◽  
Jessica P. Rickard ◽  
Simon P. de Graaf ◽  
Angela J. Crean
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Perceived Risk ◽  
Positive Relationship ◽  
Assisted Reproductive Technologies ◽  
Sperm Quality ◽  
Reproductive Technologies ◽  
Before And After ◽  
The Difference ◽  
Sperm Traits

Males can adjust sperm motility instantaneously in response to the perceived risk of sperm competition. The speed of this response suggests that sperm motility is regulated by changes in seminal plasma rather than changes in the sperm cells themselves. Hence, here we test whether inter-ejaculate variation in seminal plasma can be used to alter sperm quality prior to use in assisted reproductive technologies. We supplemented fresh ejaculates of Merino rams with seminal plasma collected from previous ‘donor’ ejaculates to test whether changes in sperm kinetics were related to the relative quality of donor to focal ejaculates. We found a positive relationship between the change in sperm traits before and after supplementation, and the difference in sperm traits between the donor and focal ejaculate. Hence, sperm motility can be either increased or decreased through the addition of seminal plasma from a superior or inferior ejaculate, respectively. This positive relationship held true even when seminal plasma was added from a previous ejaculate of the same ram, although the slope of the relationship depended on the identity of both the donor and receiver ram. These findings indicate that seminal plasma plays a key role in the control and regulation of sperm kinetics, and that sperm kinetic traits can be transferred from one ejaculate to another through seminal plasma supplementation.

scholarly journals Microphysiologic systems in female reproductive biology

2017 ◽  
Vol 242 (17) ◽  
pp. 1690-1700 ◽  
Author(s):  
Alexandria N Young ◽  
Georgette Moyle-Heyrman ◽  
J Julie Kim ◽  
Joanna E Burdette
Keyword(s):  
Reproductive Biology ◽  
Embryo Development ◽  
Reproductive Tract ◽  
New Technologies ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Female Reproductive Tract ◽  
The Body ◽  
Technological Advancement ◽  
Organ Systems

Microphysiologic systems (MPS), including new organ-on-a-chip technologies, recapitulate tissue microenvironments by employing specially designed tissue or cell culturing techniques and microfluidic flow. Such systems are designed to incorporate physiologic factors that conventional 2D or even 3D systems cannot, such as the multicellular dynamics of a tissue–tissue interface or physical forces like fluid sheer stress. The female reproductive system is a series of interconnected organs that are necessary to produce eggs, support embryo development and female health, and impact the functioning of non-reproductive tissues throughout the body. Despite its importance, the human reproductive tract has received less attention than other organ systems, such as the liver and kidney, in terms of modeling with MPS. In this review, we discuss current gaps in the field and areas for technological advancement through the application of MPS. We explore current MPS research in female reproductive biology, including fertilization, pregnancy, and female reproductive tract diseases, with a focus on their clinical applications. Impact statement This review discusses existing microphysiologic systems technology that may be applied to study of the female reproductive tract, and those currently in development to specifically investigate gametes, fertilization, embryo development, pregnancy, and diseases of the female reproductive tract. We focus on the clinical applicability of these new technologies in fields such as assisted reproductive technologies, drug testing, disease diagnostics, and personalized medicine.

Medical-biological and social significance of female infertility

2020 ◽  
pp. 29-38
Author(s):  
Essedulla M. OSMANOV ◽  
Aleksey Yu. PROKOPOV
Keyword(s):  
Birth Rate ◽  
Family Income ◽  
Assisted Reproductive Technologies ◽  
Pension System ◽  
Reproductive Technologies ◽  
Female Infertility ◽  
High Rate ◽  
Sexual Life ◽  
Social Significance ◽  
Demographic Situation

As a result of the decline in the population, Russia has faced a number of economic and social problems related to the reduction of the working-age population, as well as the need to reform the existing pension system, one of the measures aimed at improving the demographic situation is the increase in the birth rate. The aim of the study is to study the medical, biological and social significance of female infertility on the basis of a literary review. The results of the review showed a high level of infertility both in Russia and around the world. Among the main medical and biological risk factors for female infertility are the high prevalence of harmful habits, the earlier age of entry into sexual life of the high rate of sexually transmitted infections, gynaecological incidence, the high rate of abortion, the postponement of the birth of children to an older age. Social factors include low family income, poor quality of life, poor relationships with spouses and dissatisfaction with sexual intimacy. The increase in the number of families with infertility indicates an increasing need for assisted reproductive technologies. In developed countries, assisted reproductive technologies produce between 0.4 and 4.0 % children. In Russia, 160836 children were born with assisted reproductive technologies from 2007 to 2013, and the contribution of assisted reproductive technologies to annual fertility increased almost 40 times (from 0.04 to 1.55 %). The conclusion: the restoration of women's reproductive health is one of the promising directions in raising the birth rate and restoring the demographic situation.

The Reproductive Revolution

2017 ◽  
Author(s):  
Judith Daar
Keyword(s):  
Sexual Intercourse ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Culture Wars ◽  
Distinguishing Characteristic ◽  
Contemporary Culture ◽  
The World

This chapter discusses how the world of assisted reproductive technologies (ART) offers those who cannot reproduce the old-fashioned way various medical techniques aimed at achieving pregnancy by means other than sexual intercourse. By disaggregating sex from reproduction, ART is the story of both technical sophistication and social liberation. The shakeup of long-established medical, social, and familial norms has been one of ART's hallmarks, a distinguishing characteristic that often places it in the crossfire of contemporary culture wars. Though designed as mere medical techniques to overcome infertility, ART's increasing invocation by those historically deprived of reproductive opportunities invites scrutiny into its every use and its very existence.

Transforming growth factor-? (TGF?) in porcine seminal plasma

2011 ◽  
Vol 23 (6) ◽  
pp. 748 ◽  
Author(s):  
Sean O'Leary ◽  
David T. Armstrong ◽  
Sarah A. Robertson
Keyword(s):  
Growth Factor ◽  
Seminal Plasma ◽  
Reproductive Tract ◽  
Transforming Growth Factor ◽  
Seminal Fluid ◽  
Transforming Growth Factor Β ◽  
Interferon Γ ◽  
Female Reproductive Tract ◽  
Active Constituent ◽  
Sperm Density

Bioactive factors in seminal plasma induce cellular and molecular changes in the female reproductive tract after coitus. An active constituent of seminal plasma in mice and humans is the potent immune-modulating cytokine transforming growth factor-β (TGFβ). To investigate whether TGFβ is present in boar seminal plasma, TGFβ1 and TGFβ2 were measured by immunoassay. High levels of TGFβ1 and TGFβ2 were detected in 100% of seminal fluid samples from 73 boars. Both were predominantly in the active, not latent form. Interferon-γ (IFNγ) and lipopolysaccharide (LPS), agents that interact with TGFβ signalling, were detectable in 5% and 100% of samples, respectively. TGFβ1 and TGFβ2 concentrations varied widely between boars, but correlated with each other and with sperm density, and remained relatively constant within individual boars over a 6-month period. Frequent semen collection substantially diminished the concentration of both TGFβ isoforms. Using retrospective breeding data for 44 boars, no correlation between TGFβ content and boar reproductive performance by artificial insemination (AI) with diluted semen was found. It is concluded that TGFβ is abundant in boar seminal plasma, leading to the speculation that, in pigs, TGFβ may be a male–female signalling agent involved in immune changes in the female reproductive tract elicited by seminal fluid.

scholarly journals Ram seminal plasma and its functional proteomic assessment

Reproduction ◽  
2019 ◽  
Vol 157 (6) ◽  
pp. R243-R256 ◽  
Author(s):  
T Leahy ◽  
J P Rickard ◽  
N C Bernecic ◽  
X Druart ◽  
S P de Graaf
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Reproductive Tract ◽  
Reproductive Technologies ◽  
Female Reproductive Tract ◽  
Sperm Function ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

Ejaculation results in the confluence of epididymal spermatozoa with secretions of the accessory sex glands. This interaction is not a prerequisite for fertilisation success, but seminal factors do play a crucial role in prolonging the survival of spermatozoa bothin vitroandin vivoby affording protection from handling induced stress and some selective mechanisms of the female reproductive tract. Reproductive biologists have long sought to identify specific factors in seminal plasma that influence sperm function and fertility in these contexts. Many seminal plasma proteins have been identified as diagnostic predictors of sperm function and have been isolated and appliedin vitroto prevent sperm damage associated with the application of artificial reproductive technologies. Proteomic assessment of the spermatozoon, and its surroundings, has provided considerable advances towards these goals and allowed for greater understanding of their physiological function. In this review, the importance of seminal plasma will be examined through a proteomic lens to provide comprehensive analysis of the ram seminal proteome and detail the use of proteomic studies that correlate seminal plasma proteins with ram sperm function and preservation ability.

scholarly journals To understand the reproductive tract microbiome associated with infertility through metagenomics analysis

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Manisha Vajpeyee ◽  
Lokendra Bahadur Yadav ◽  
Shivam Tiwari ◽  
Parikshit Tank
Keyword(s):  
Reproductive Tract ◽  
Assisted Reproductive Technologies ◽  
Live Birth Rate ◽  
Reproductive Technologies ◽  
Female Reproductive Tract ◽  
Human Reproduction ◽  
Main Body ◽  
Fertility Treatments ◽  
Vaginal Tract ◽  
Human Illness

Abstract Background Knowledge of the microbiome is in its infancy in health and human illness, especially concerning human reproduction. We will be better able to treat dysbiosis of the reproductive tract clinically if it is better explained and understood. It has been shown that altered vaginal microbiota affects parturition, and its function is uncertain in assisted reproductive technologies. However, the effects of recognized microbes such as Mycoplasma tuberculosis, Chlamydia trachomatis, and Neisseria gonorrhoeae are well established, resulting in subclinical changes which are considered to be risk factors for infertility and poor reproductive outcomes. Main body Recent studies indicate that the vaginal tract comprises several different organisms of the microbiome. Some microbiota can play an important role not only in the reproductive tract but also in overall health. The microbiome of the female reproductive tract has been identified mainly based on studies that examine vaginal samples across many reproductive technologies, using a metagenomics approach. Conclusion Alteration of reproductive tract microbiota or presence of certain microbiota irrespective of the level of pathogenicity may interfere with fertilization, implantation, and subsequent embryo development. This may lead to failed fertility treatments and reduced live birth rate (LBR).

242. LIF expression is induced in the mouse oviduct following activation by seminal factors

2005 ◽  
Vol 17 (9) ◽  
pp. 96 ◽  
Author(s):  
M. J. Jasper ◽  
J. J. Bromfield ◽  
R. Skinner ◽  
S. A. Robertson
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Seminal Plasma ◽  
Early Pregnancy ◽  
Transforming Growth Factor ◽  
Female Reproductive Tract ◽  
Leukaemia Inhibitory Factor ◽  
Tissue Remodelling ◽  
Gm Csf ◽  
Epidermal Growth

A precisely regulated sequence of molecular and cellular changes occurs in the female reproductive tract in early pregnancy to facilitate development of the embryo and its successful implantation. Cytokine–leukocyte networks are integral in the tissue remodelling and immuno-regulatory processes underpinning successful implantation. Seminal factors are implicated in activating expression of cytokine genes and inflammatory leukocyte recruitment in the uterus, but whether semen-induced effects extend to the oviduct to influence blastocyst development has not been examined. The aim of this study was to quantitate the expression of mRNA encoding epidermal growth factor (EGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), heparin-binding epidermal growth factor-like growth factor (HB-EGF), insulin-like growth factor (IGFII), leukaemia inhibitory factor (LIF), tumour necrosis factor (TNFα), transforming growth factor (TGFα) and TGFβ from oviducts collected from mice at oestrus and on day 1 of pregnancy, after mating with intact, seminal plasma deficient (svx) and vasectomised (vas) mice. Total RNA was extracted, DNAse treated, reverse transcribed into cDNA, and quantified by real-time PCR using SYBR Green chemistry. All cytokine-specific primers were designed using GenBank sequences and data were normalised to β-actin mRNA expression. Expression of LIF mRNA was induced following mating with intact or vas males, but not svx males, showing that LIF mRNA is induced by factors present in seminal plasma. mRNAs encoding EGF, GM-CSF, HB EGF, IGFII, TNFα, TGFα and TGFβ were all detected in oviduct cDNA collected from oestrus and day 1 mice. These data support the proposal that cytokine-leukocyte networks shown previously to be operative in the uterus extend to the oviduct, and are influenced by exposure to seminal plasma at mating. The cytokines expressed in the oviduct during early pregnancy are likely to be key regulators of embryo growth and development.

scholarly journals Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies

2013 ◽  
Vol 45 (14) ◽  
pp. 577-589 ◽  
Author(s):  
S. Clay Isom ◽  
John R. Stevens ◽  
Rongfeng Li ◽  
William G. Spollen ◽  
Lindsay Cox ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Transforming Growth Factor ◽  
Assisted Reproductive Technologies ◽  
Expression Profiles ◽  
Reproductive Technologies ◽  
Fertilization In Vitro ◽  
Oocyte Activation ◽  
Attachment Development

Substantial mortality of in vitro manipulated porcine embryos is observed during peri-attachment development. Herein we describe our efforts to characterize the transcriptomes of embryonic disc (ED) and trophectoderm (TE) cells from porcine embryos derived from in vivo fertilization, in vitro fertilization (IVF), parthenogenetic oocyte activation (PA), and somatic cell nuclear transfer (SCNT) on days 10, 12, and 14 of gestation. The IVF, PA, and SCNT embryos were generated with in vitro matured oocytes and were cultured overnight in vitro before being transferred to recipient females. Sequencing of cDNA from the resulting embryonic samples was accomplished with the Genome Analyzer IIx platform from Illumina. Reads were aligned to a custom-built swine transcriptome. A generalized linear model was fit for ED and TE samples separately, accounting for embryo type, gestation day, and their interaction. Those genes with significant differences between embryo types were characterized in terms of gene ontologies and KEGG pathways. Transforming growth factor-β signaling was downregulated in the EDs of IVF embryos. In TE cells from IVF embryos, ubiquitin-mediated proteolysis and ErbB signaling were aberrantly regulated. Expression of genes involved in chromatin modification, gene silencing by RNA, and apoptosis was significantly disrupted in ED cells from SCNT embryos. In summary, we have used high-throughput sequencing technologies to compare gene expression profiles of various embryo types during peri-attachment development. We expect that these data will provide important insight into the root causes of (and possible opportunities for mitigation of) suboptimal development of embryos derived from assisted reproductive technologies.

scholarly journals Extracellular Vesicles in the Oviduct: Progress, Challenges and Implications for the Reproductive Success

2019 ◽  
Vol 6 (2) ◽  
pp. 32 ◽  
Author(s):  
Almiñana ◽  
Bauersachs
Keyword(s):  
Extracellular Vesicles ◽  
Reproductive Tract ◽  
Assisted Reproductive Technologies ◽  
Current Knowledge ◽  
Cell Communication ◽  
Reproductive Technologies ◽  
Early Embryo ◽  
Female Reproductive Tract ◽  
Functional Aspects ◽  
Reproductive Events

The oviduct is the anatomical part of the female reproductive tract where the early reproductive events take place, from gamete transport, fertilization and early embryo development to the delivery of a competent embryo to the uterus, which can implant and develop to term. The success of all these events rely upon a two-way dialogue between the oviduct (lining epithelium and secretions) and the gametes/embryo(s). Recently, extracellular vesicles (EVs) have been identified as major components of oviductal secretions and pointed to as mediators of the gamete/embryo-maternal interactions. EVs, comprising exosomes and microvesicles, have emerged as important agents of cell-to-cell communication by the transfer of biomolecules (i.e., mRNAs, miRNAs, proteins) that can modulate the activities of recipient cells. Here, we provide the current knowledge of EVs in the oviductal environment, from isolation to characterization, and a description of the EVs molecular content and associated functional aspects in different species. The potential role of oviductal EVs (oEVs) as modulators of gamete/embryo-oviduct interactions and their implications in the success of early reproductive events is addressed. Lastly, we discuss current challenges and future directions towards the potential application of oEVs as therapeutic vectors to improve pregnancy disorders, infertility problems and increase the success of assisted reproductive technologies.

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