scholarly journals Hepatoprotective effect on methanolic extracts of Tagetes erecta leaves and Tridax procumbens against drug induced hepatic injury

2021 ◽  
Vol 12 (2) ◽  
pp. 1415-1421
Author(s):  
Aithamraju Satish Chandra ◽  
Shanmugapandiyan P
Keyword(s):  
Hepatic Injury ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Vehicle Control ◽  
Tagetes Erecta ◽  
Drug Induced ◽  
Methanolic Extracts ◽  
Tridax Procumbens ◽  
Biochemical Level ◽  
Hepatic Markers

The current study was undertaken to evaluate the preclinical efficacy of methanolic extract of Tagetes erecta (METE) and methanolic extract of Tridax procumbens (METP) against Isoniazid and Rifampicin (INH-RIF) induced hepatic injury. Animals were randomly divided into seven groups, vehicle (control) or INH-RIF (50 mg/kg, i.p.), METE (200 and 400mg/kg, p.o.), METP (200 and 400mg/kg, p.o.) and standard silymarin for 28 days. INH-RIF intoxicated rats displayed significant (p<0.05) elevation in serum hepatic markers, lipid peroxidation and decrease in antioxidants like SOD, CAT, Gpx and GSH in liver tissue. Treatment with METE & METP (200 and 400 mg/kg, p.o.) restored the altered biochemical level to normalcy. Thus, the outcome of the study reveals that METE & METP showed promising hepatoprotective activity in INH-RIF induced hepatic damage mediated by its membrane stabilizing and antioxidant effect. All the methanolic plant extracts produce significant hepatoprotective activity against drug induced hepatic injury.

scholarly journals Hepatoprotective Effect of Corrigiola telephiifolia Pourr. Root Methanolic Extracts against CCl4-Induced Hepatic Damage in Mice

2021 ◽  
Vol 12 (2) ◽  
pp. 2489-2502
Keyword(s):  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Hepatic Damage ◽  
Hepatoprotective Effect ◽  
Histopathological Analysis ◽  
Medicinal And Aromatic Plants ◽  
Bioactive Substances ◽  
Methanolic Extracts ◽  
Hepatic Cells

Liver disease is a dysfunction that affects all or part of the liver and can lead to death. While the use of medicinal and aromatic plants has been a source of bioactive substances with hepatoprotective properties. This study aims to evaluate the hepatoprotective effect of the methanolic extract of Corrigiola telephiifolia roots on hepatic damage induced by carbon tetrachloride (CCl4) in mice. Animals were treated daily with C. telephiifolia methanolic extract (CTME) at doses of 5 and 10 mg/kg b.w for 60 days. In addition, CCl4 was injected (1 mL/kg, i.p.) for its hepatotoxic effects. At the end of the experiment, the blood of all animals was collected to evaluate biochemical parameters and the liver for histopathological analysis. The administration of CTME showed significant hepatoprotective activity by improving the biochemical parameters and the histological appearance of hepatic cells induced by CCl4. Consequently, C. telephiifolia could be used in the prevention and/or treatment of liver intoxication.

scholarly journals In Vivo Studies of Inoculated Plants and In Vitro Studies Utilizing Methanolic Extracts of Endophytic Streptomyces sp. Strain DBT34 Obtained from Mirabilis jalapa L. Exhibit ROS-Scavenging and Other Bioactive Properties

2020 ◽  
Vol 21 (19) ◽  
pp. 7364 ◽  
Author(s):  
Ajit Kumar Passari ◽  
Vincent Vineeth Leo ◽  
Garima Singh ◽  
Loknath Samanta ◽  
Heera Ram ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Radical Scavenging ◽  
Vehicle Control ◽  
Antioxidant Potential ◽  
Ros Scavenging ◽  
Streptomyces Sp ◽  
Methanolic Extracts ◽  
Mirabilis Jalapa ◽  
Ic50 Values

Reactive oxygen species (ROS) and other free radicals cause oxidative damage in cells under biotic and abiotic stress. Endophytic microorganisms reside in the internal tissues of plants and contribute to the mitigation of such stresses by the production of antioxidant enzymes and compounds. We hypothesized that the endophytic actinobacterium Streptomyces sp. strain DBT34, which was previously demonstrated to have plant growth-promoting (PGP) and antimicrobial properties, may also have a role in protecting plants against several stresses through the production of antioxidants. The present study was designed to characterize catalase and superoxide dismutase (SOD), two enzymes involved in the detoxification of ROS, in methanolic extracts derived from six endophytic actinobacterial isolates obtained from the traditional medicinal plant Mirabilis jalapa. The results of a preliminary screen indicated that Streptomyces sp. strain DBT34 was the best overall strain and was therefore used in subsequent detailed analyses. A methanolic extract of DBT34 exhibited significant antioxidant potential in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assays. The cytotoxicity of DBT34 against liver hepatocellular cells (HepG2) was also determined. Results indicated that methanolic extract of Streptomyces sp. strain DBT34 exhibited significant catalase and SOD-like activity with 158.21 U resulting in a 55.15% reduction in ROS. The IC50 values of a crude methanolic extract of strain DBT34 on DPPH radical scavenging and ABTS radical cation decolorization were 41.5 µg/mL and 47.8 µg/mL, respectively. Volatile compounds (VOC) were also detected in the methanolic extract of Streptomyces sp. strain DBT34 using GC-MS analysis to correlate their presence with bioactive potential. Treatments of rats with DBT34 extract and sitagliptin resulted in a significant (p ≤ 0.001) reduction in total cholesterol, LDL-cholesterol, and VLDL-cholesterol, relative to the vehicle control and a standard diabetic medicine. The pancreatic histoarchitecture of vehicle control rats exhibited a compact volume of isolated clusters of Langerhans cells surrounded by acinies with proper vaculation. An in-vivo study of Streptomyces sp. strain DBT34 on chickpea seedlings revealed an enhancement in its antioxidant potential as denoted by lower IC50 values for DPPH and ABTS radical scavenging activity under greenhouse conditions in relative comparison to control plants. Results of the study indicate that strain DBT34 provides a defense mechanism to its host through the production of antioxidant therapeutic agents that mitigate ROS in hosts subjected to biotic and abiotic stresses.

scholarly journals HEPATOPROTECTIVE EFFECTS OF POLYHERBAL FORMULATION AGAINST CARBON TETRACHLORIDE-INDUCED HEPATIC INJURY IN ALBINO RATS: A TOXICITY SCREENING APPROACH

2016 ◽  
Vol 10 (1) ◽  
pp. 192 ◽  
Author(s):  
Bhumika Yogi ◽  
Ashutosh Mishra
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Calotropis Procera ◽  
Albino Rats ◽  
Gymnema Sylvestre ◽  
Toxicity Screening ◽  
Lawsonia Inermis ◽  
Polyherbal Formulation

ABSTRACTObjective: To investigate the hepatoprotective activity of aqueous methanolic extract of polyherbal formulation and individual extracts of Calotropisprocera, Gymnema sylvestre, and Lawsonia inermis leaves.Methods: The hepatoprotective activity of the formulation and extracts were assessed on the basis of histopathological changes in liver of carbontetrachloride-induced hepatotoxic rats.Results: The result of present studies strongly indicates that the hepatoprotective property of polyherbal formulation as evidenced by less damagedhepatocytes cells.Conclusion: Polyherbal formulation revealed excellent hepatoprotective profile as compared to individual plants due to synergistic effect producedby different phytochemicals present in the formulation.Keywords: Calotropis procera, Gymnema sylvestre, Lawsonia inermis, Carbon tetrachloride.

scholarly journals In-vivo Hepatoprotective and Antioxidant Activities of Sphaeranthus amaranthoides Burm.f. Against Anti-Tubercular Drugs Induced Hepatotoxicity in Rats

2021 ◽  
pp. 15-23
Author(s):  
M. R. Vinayakamurthi ◽  
J. Anbu Jeba Sunilson ◽  
A. V. Anita Gnana Kumari ◽  
U. Aathilakshmi
Keyword(s):  
Antioxidant Activity ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Liver Homogenate ◽  
Hepatoprotective Activity ◽  
Thiobarbituric Acid Reactive Substance ◽  
Enzymatic Antioxidants ◽  
Drug Induced

Aim: To evaluate the hepatoprotective and antioxidant activity of Sphaeranthus amaranthoides Burm.f. against isoniazid (INH) and rifampicin (RIF) induced hepatotoxicity in rats. Study Design: Experimental study. Place and Duration: Research lab, Department of Siddha Medicine, Tamil University, Thanjavur, India, between March 2018 and November 2019. Methodology: Liver toxicity was induced by antitubercular drugs (Isoniazid; INH+Rifampicin; RIF) at a dose level of 50+100 mg/kg each, p.o for 15 days. Petroleum ether, Chloroform, Methanol, Aqueous extracts of Sphaeranthus amaranthoides Burm.f. (S. amaranthoides) (200 and 400 mg/kg bt.wt.) were administered orally once daily for 15 days. The hepatoprotective activity was assessed using various biochemical parameters SGOT, SGPT, ALP, bilirubin, total protein, albumin, total cholesterol, total bilirubin, direct bilirubin and LDH. The antioxidant activities such as the enzymatic activity of superoxide dismutase (SOD), and catalase (CAT), and the level of lipidperoxidation as thiobarbituric acid reactive substance (TBA-RS) were measured in liver homogenates and histological examinations were carried out to assess hepatoprotective activity. For Statistical analysis, the values were subjected to one way analysis of variance (ANOVA) followed by Tukey multiple compare test. Results were considered statistically significant when P<0.05. Results: The treatment with methanolic extract (400 mg/kg bt.wt.) of S. amaranthoides significantly prevented drug-induced increase in serum levels of liver enzymes (P<0.001). The antioxidant activity of a dose of 400 mg/kg of S. amaranthoides significantly prevented the decreases in the activity of enzymatic antioxidants (CAT & SOD) (P<0.01 and P<0.001) and inhibited the elevation of lipid peroxidation (TBA-RS) in the liver homogenate. Histopathology of liver tissue showed that S. amaranthoides attenuated the hepatocellular necrosis, regeneration and repair of cells toward normal. Conclusion: The methanolic extract of S. amaranthoides showed significant hepatoprotectivity and antioxidant activity against INH + RIF Anti TB drugs.

Antioxidant and hepatoprotective activity of Tragopogon porrifolius methanolic extract

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
M Mroueh ◽  
C Daher ◽  
M El Sibai ◽  
C Tenkerian
Keyword(s):  
Methanolic Extract ◽  
Hepatoprotective Activity

Bergenin Exhibits Hepatoprotective Activity Against Ethanol-Induced Oxidative Stress in ICR Mice

2019 ◽  
Vol 18 (4) ◽  
pp. 297-302
Author(s):  
Sriset Yollada ◽  
Chatuphonprasert Waranya ◽  
Jarukamjorn Kanokwan
Keyword(s):  
Reactive Oxygen Species ◽  
Gallic Acid ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Hepatic Injury ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Hepatoprotective Activity ◽  
Oxygen Species ◽  
Hepatic Glutathione

Bergenin is a C-glucoside derivative of gallic acid but its antioxidant and hepatoprotective effects have not previously been compared with gallic acid. Male ICR mice were administered bergenin (10, 50, and 250 mg/kg/day) or gallic acid (100 mg/kg/day) for 7 consecutive days before a single administration of ethanol (5 g/kg). Liver sections were histopathologically examined. Aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde levels were determined in plasma. Total glutathione, reduced glutathione, and oxidized glutathione levels were determined in liver homogenates. Ethanol induced hepatic injury with prominent histopathological markers including nuclear pyknosis and necrotic areas and this accorded with increases in the plasma levels of aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde. Moreover, ethanol disturbed hepatic glutathione homeostasis by reducing glutathione stores. Hepatic injury in the ethanol-induced mice was prevented with bergenin and gallic acid by significant decreases in plasma aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde levels and restoration of the hepatic glutathione profile through an increase in the reduced glutathione/oxidized glutathione ratio. Bergenin at 10 mg/kg/day showed comparable hepatoprotective activity to gallic acid in an ethanol-induced mouse model of oxidative stress. Therefore, bergenin might be a promising candidate for further development as a novel hepatoprotective product.

Investigating the Antioxidant Potential of Garlic (Allium sativum) Extracts Through Different Extraction Modes

2019 ◽  
Vol 15 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Kanza A. Awan ◽  
Masood S. Butt ◽  
Iahtisham Ul Haq ◽  
Hafiz A.R. Suleria
Keyword(s):  
Allium Sativum ◽  
Total Phenolics ◽  
Methanolic Extract ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Time Intervals ◽  
Methanolic Extracts ◽  
Maximum Extraction ◽  
Acid Potential ◽  
The Ideal

Background: Garlic (Allium sativum) possesses health enhancing abilities due to the presence various phytoceutics moities. The current research was deigned to explore the phytochemicals and antioxidant capacity of Pakistani garlic. Methods: Garlic extracts were obtained using methanol, hexane and ethyl acetate at different time intervals (35, 50 and 65 min) followed by their polyphenols and flavonoid content determination. Afterwards, the antioxidant potential was also determined. Results: The outcomes revealed that the methanolic extracts obtained at 50 min extraction time showed maximum total phenolics as 60.38±0.23 mg GAE/100g and flavonoids as 58.45&amp;#177;1.24 mg/100g. Similarly, the highest DPPH activity (61.59±1.58%) and &amp;#946;-carotene and linoleic acid potential (64.96&amp;#177;1.72%) were also observed for methanolic extract. Conclusion: Inferences were made that Pakistani garlic contains myriad of phenolics and flavonoids but the extraction of these components depends upon the solvent/time combination. In this study, methanol proved to be the ideal solvent for the maximum extraction of phytochemicals from garlic.

scholarly journals Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Fariba Heshmati Afshar ◽  
Masumeh Zadehkamand ◽  
Zahra Rezaei ◽  
Abbas Delazar ◽  
Vahideh Tarhriz ◽  
...  
Keyword(s):  
Cell Line ◽  
Proliferative Activity ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Flavonoid Glycosides ◽  
Control Group ◽  
Chemical Compositions ◽  
Preparative Hplc ◽  
Cytotoxicity Activity ◽  
Methanolic Extracts

Abstract Background Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by 1H and 13C NMR. Results The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides. Conclusion Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

scholarly journals Drug-Induced Photosensitivity—From Light and Chemistry to Biological Reactions and Clinical Symptoms

2021 ◽  
Vol 14 (8) ◽  
pp. 723
Author(s):  
Justyna Kowalska ◽  
Jakub Rok ◽  
Zuzanna Rzepka ◽  
Dorota Wrześniok
Keyword(s):  
Defense Mechanisms ◽  
Clinical Symptoms ◽  
Chemical Properties ◽  
Additional Treatment ◽  
Cosmetic Products ◽  
Aesthetic Value ◽  
Drug Usage ◽  
Drug Induced ◽  
Biochemical Level ◽  
The Aesthetic

Photosensitivity is one of the most common cutaneous adverse drug reactions. There are two types of drug-induced photosensitivity: photoallergy and phototoxicity. Currently, the number of photosensitization cases is constantly increasing due to excessive exposure to sunlight, the aesthetic value of a tan, and the increasing number of photosensitizing substances in food, dietary supplements, and pharmaceutical and cosmetic products. The risk of photosensitivity reactions relates to several hundred externally and systemically administered drugs, including nonsteroidal anti-inflammatory, cardiovascular, psychotropic, antimicrobial, antihyperlipidemic, and antineoplastic drugs. Photosensitivity reactions often lead to hospitalization, additional treatment, medical management, decrease in patient’s comfort, and the limitations of drug usage. Mechanisms of drug-induced photosensitivity are complex and are observed at a cellular, molecular, and biochemical level. Photoexcitation and photoconversion of drugs trigger multidirectional biological reactions, including oxidative stress, inflammation, and changes in melanin synthesis. These effects contribute to the appearance of the following symptoms: erythema, swelling, blisters, exudation, peeling, burning, itching, and hyperpigmentation of the skin. This article reviews in detail the chemical and biological basis of drug-induced photosensitivity. The following factors are considered: the chemical properties, the influence of individual ranges of sunlight, the presence of melanin biopolymers, and the defense mechanisms of particular types of tested cells.

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