Bacterial Contamination of Acrylic Resin Complete Denture : In Vitro Study

2018 ◽  
Vol 30 (2) ◽  
pp. 1-4
Author(s):  
Cheman Abdulrahman Al Jmoor
Keyword(s):  
Bacterial Contamination ◽  
Health Hazard ◽  
In Vitro Study ◽  
Acrylic Resin ◽  
Complete Denture ◽  
Initial Stage ◽  
Group 2 ◽  
Mouth Wash ◽  
Two Stages

Background: Cross contamination of dental appliances in the dental clinics and laboratories may potentially be a health hazard to the dental team and the patient. This study aimed to evaluate bacterial contamination of acrylic complete denture as received from dental laboratory before delivery to the patient, and then to evaluate the effectiveness of disinfection with 2% chlorhexidine and Kin denture cleaner tablet. Materials and methods: 45 newly made upper complete dentures undergone biaacterial examination for contamination before delivered to the patient. Samples were examined in two stages, first after finishing and polishing; when collected from the laboratory and before inserting to the patient mouth, second; after the samples were immersed in 2 different disinfectant materials, 2% chlorhexidine mouth wash and Kin denture cleaner tablet. After initial stage, the dentures were divided into 3 groups. Group 1 immersed in Kin denture cleaner tablet for 10 minutes, group 2 immersed in 2% chlorhexidine mouth wash for 10 minutes and group 3 immersed for 20 minutes in 2% chlorhexidine. Data were analyzed with a computer-run statistical program (IBM SPSS Version 23). Results: High score of bacterial contamination was found initially in the sample collected from dental laboratory. Significant reduction in the colonies number was noticed after immersing the dentures in 2% Chlorhexidine and Kin denture cleaner tablets for10 minutes. There was nearly no contamination found with samples immersed in 2% chlorhexidine for 20 minutes. Conclusion: Dental laboratory is a main source of microbial contamination. Immersion of the dental prosthesis in disinfectant materials is essential before inserting into the patient mouth. 2%chlorhexidine mouth wash was more effective as disinfection material as compared to Kin denture cleaner tablet.

Implants With Internal Hexagon and Conical Implant-Abutment Connections: An In Vitro Study of the Bacterial Contamination

2014 ◽  
Vol 40 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Simonetta D'Ercole ◽  
Antonio Scarano ◽  
Vittoria Perrotti ◽  
Jorge Mulatinho ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bacterial Suspension ◽  
Inflammatory Reactions ◽  
Implant Abutment ◽  
Group 2 ◽  
Group 1 ◽  
Internal Connections

Prevention of microbial leakage at the implant-abutment junction is a major challenge for the construction of 2-stage implants in order to minimize inflammatory reactions and to maximize bone stability at the implant neck. The aim of the present in vitro study was an evaluation of the leakage observed over a period of 28 days in Cone Morse taper internal connections and in screwed-abutments connections. In the present study 10 specimens of Cone Morse (Group 1) and 10 of internal hexagon (Group 2) implants were used. The inner parts of 5 implants per group were inoculated with Pseudomonas aeruginosa (PS) suspension and 5 implants per group with Aggregatibacter actinomycetemcomitans (AA). The possible penetration of bacterial suspension into the surrounding solution was determined by the observation of turbidity of the broth. In Group 1, bacterial contamination was found in 3 out of 5 implant-abutment assemblies seeded with the PS and in 2 samples out of 5 in the assemblies seeded with AA, with a total of leaked assemblies in this group of 5 out of 10. In Group 2, bacterial contamination was found in 4 out of 5 implant-abutment assemblies seeded with the PS, and in 4 out of 5 samples seeded with AA, with a total of leaked assemblies of 8 out of 10. The present data confirm the reported high permeability to bacterial leakage of screw-retained abutment connections, and the lower infiltration rates—although not significantly—of Cone Morse taper internal connections.

scholarly journals Comparison of Fracture Resistance in Thermal and Self-Curing Acrylic Resins—An In Vitro Study

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1234
Author(s):  
António Sérgio Silva ◽  
Aurora Carvalho ◽  
Pedro Barreiros ◽  
Juliana de Sá ◽  
Carlos Aroso ◽  
...  
Keyword(s):  
Fracture Resistance ◽  
Research Work ◽  
Testing Machine ◽  
In Vitro Study ◽  
Dimensional Accuracy ◽  
Acrylic Resin ◽  
Thermosetting Resins ◽  
Acrylic Resins ◽  
The Difference

Thermal and self-curing acrylic resins are frequently and versatilely used in dental medicine since they are biocompatible, have no flavor or odor, have satisfactory thermal qualities and polishing capacity, and are easy and fast. Thus, given their widespread use, their fracture resistance behavior is especially important. In this research work, we comparatively analyzed the fracture resistance capacity of thermo and self-curing acrylic resins in vitro. Materials and Methods: Five prosthesis bases were created for each of the following acrylic resins: Lucitone®, ProBase®, and Megacryl®, which were submitted to different forces through the use of the CS® Dental Testing Machine, usually mobilized in the context of fatigue tests. To this end, a point was defined in the center of the anterior edge of the aforementioned acrylic resin bases, for which the peak tended until a fracture occurred. Thermosetting resins were, on average, more resistant to fracture than self-curable resins, although the difference was not statistically significant. The thermosetting resins of the Lucitone® and Probase® brands demonstrated behavior that was more resistant to fracture than the self-curing homologues, although the difference was not statistically significant. Thermosetting resins tended to be, on average, more resistant to fracture and exhibited the maximum values for impact strength, compressive strength, tensile strength, hardness, and dimensional accuracy than self-curing resins, regardless of brand.

scholarly journals An in-vitro study to evaluate the accuracy of dies obtained from dual arch impression trays using addition silicone impression material and tray combinations

2015 ◽  
Vol 12 (2) ◽  
pp. 111-117
Author(s):  
P K Parajuli ◽  
S George ◽  
V Shenoy
Keyword(s):  
In Vitro Study ◽  
Dimensional Accuracy ◽  
Acrylic Resin ◽  
Simultaneous Recording ◽  
T Test ◽  
Impression Material ◽  
Tooth Preparation ◽  
Made In ◽  
Plastic Trays

Background: Dual-arch impression technique allows the simultaneous recording of  tooth preparation, opposing anatomic tooth and maxillomandibular relationship. The  accuracy of reproduction of this easy and quick technique, however, has not been  studied in detail in past. Objective: To compare the accuracy of the impressions  made by using the same impression material in dual arch plastic trays, dual-arch  metal trays and acrylic resin custom trays. Methods: The dies obtained from the  addition silicone impressions made in dual-arch plastic trays, dual-arch metal trays  and full arch acrylic resin custom trays were compared for the dimensional accuracy  with the prepared typodont tooth as a control. Student’s paired t-test and unpaired  t-test were used for the data analyses using the Statistical Package for Social Studies  (SPSS) version 11.5. Results: The dies obtained from all the impression combinations  showed increased dimension (acrylic resin custom trays 9.4 mm±0.048, dual-arch  plastic trays 9.5 mm±0.035, dual-arch metal trays 9.41 mm±0.017) as compared to  the dimension of control (9.39 mm±0.007). Conclusion: All the tray-impression  material combinations showed variable accuracies. Full arch acrylic resin trays  resulted in greatest accuracy whereas dual-arch plastic trays the least accuracy.Health Renaissance 2014;12(2):  pp: 111-117

scholarly journals Effectiveness of a silicon-based root canal sealer for filling of simulated lateral canals

2007 ◽  
Vol 18 (1) ◽  
pp. 20-23 ◽  
Author(s):  
João Vicente Baroni Barbizam ◽  
Matheus Souza ◽  
Doglas Cecchin ◽  
Jakob Dabbel
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Root Canal Sealer ◽  
Human Teeth ◽  
Significance Level ◽  
Working Length ◽  
Group 3 ◽  
Group 2 ◽  
Silicon Based

The purpose of this in vitro study was to evaluate the ability of a silicon-based root canal sealer, compared to zinc oxide and eugenol and an epoxy resin-based sealers, for filling of simulated lateral canals. Thirty extracted single-rooted human teeth were selected, conventional access was made and the working length was established 1 mm from the apical foramen. Three simulated lateral canals, one in each root third (coronal, middle and apical) were prepared in both the mesial and distal surfaces of each tooth using a size 15 reamer adapted to a low-speed handpiece. Each root canal was instrumented using ProTaper rotary files up to file F3 at the working length, and then irrigated with 2.5% NaOCl followed by EDTA. The teeth were assigned to 3 groups (n=10), according to the root canal sealer: Roeko Seal (Group 1), Sealer 26 (group 2) and Grossman's sealer (Group 3). Gutta-percha cold lateral condensation technique was performed in all groups. Postoperative radiographs were taken and the images were projected for evaluation of the quality of lateral canal filling. Data were submitted to statistical analysis by Kruskal Wallis test at 5% significance level. The results showed that Grossman's sealer filled a larger number of lateral canals than Roeko Seal (p<0.05) and Sealer 26 (p<0.01). It may be concluded that Roeko Seal silicone-based root canal sealer was not as effective as the Grossman's sealer for filling of simulated lateral canals. The lateral canals localized in the apical third of the root were more difficult to be filled.

scholarly journals Comparative Evaluation of Cleaning Efficacy of Three Different Single File Systems: An In Vitro Study

2021 ◽  
pp. 26-31
Author(s):  
Deebah Choudhary
Keyword(s):  
File Systems ◽  
In Vitro Study ◽  
Single File ◽  
Working Length ◽  
Group 3 ◽  
Group 2 ◽  
Cleaning Efficacy ◽  
Scanning Electron ◽  
Group 1

Aim: The aim of this study was to evaluate the canal cleaning efficacy of these three file systems using scanning electron microscopy. Place and Duration of Study: The study was conducted in the Department of Conservative dentistry and Endodontics, Institute of Dental Sciences Sehora, between October 2020 and December 2020. Materials and Methods: Access cavity preparation was performed on sixty extracted human mandibular premolar teeth and working length was determined. The samples were randomly divided into three groups (n=20) depending upon the file system used i.e. Group 1 (Reciproc Blue), Group 2 (Waveone Gold) and Group 3 (F360). Samples were split into two halves by creating longitudinal grooves on the buccal and lingual surfaces. The samples were sputter-coated with gold and examined under scanning electron microscope at 5000X. The dentinal wall of root canal at coronal, middle and apical thirds of each sample were evaluated for the presence of determining the canal cleanliness and then analyzed using a five-score index. Results: The results of this study revealed that Group 1 (Reciproc Blue) exhibited better cleaning efficacy than samples of Group 2 (WaveOne Gold) and Group 3 (F360) at different locations in the canal i.e. coronal, middle and apical. The mean debris present was highest in coronal area for both group 2 and group 3 i.e. 2.1 and least was seen in apical area of group 1 i.e. 0.3. (p<0.05) Conclusion: Reciproc Blue single-file showed highest cleaning efficacy followed by Waveone Gold and F360. Reciproc file also showed effective cleaning in the apical third of the canal.

scholarly journals Biochemical Interactions Between Polymeric Resins Used for Occlusal Splints and Saliva A pilot study comparing the CAD/CAM technology and the conventional approach

2019 ◽  
Vol 56 (2) ◽  
pp. 409-412 ◽  
Author(s):  
Marina Melescanu-Imre ◽  
Mihaela Pantea ◽  
Alexandra Totan ◽  
Ana Maria Cristina Tancu ◽  
Maria Greabu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
In Vitro Study ◽  
Acrylic Resin ◽  
Occlusal Splints ◽  
Oral Environment ◽  
Cad Cam ◽  
3D Printed ◽  
Polymeric Resins

The CAD/CAM technology has been successfully integrated in clinical and laboratory aspects of dental medicine. The present in vitro study focuses on the biochemical interactions between saliva and three types of polymeric resins for occlusal splints. Dental material samples were produced from 3D printed, milled and self-cured resins and were incubated with saliva samples from 20 healthy volunteers. The results showed that the 3D printed and milled polymeric resins did not produce any significant changes in oxidative stress parameters (uric acid, TAC, GGT, OXSR-1) or inflammatory markers (IL-2, IL-6). On the other hand, the self-cured acrylic resin produced a significant decrease in the salivary TAC and uric acid, the most important antioxidants in saliva, affecting the capacity of saliva to protect the oral environment against oxidative stress.

scholarly journals Comparative Evaluation of Dimensional Accuracy, Surface Characteristics, and Load Deflection Attributes of 0.019" × 0.025" Stainless Steel Wires Sourced from Six Different Commercial Houses: An In Vitro Study

2020 ◽  
Vol 54 (4) ◽  
pp. 311-320
Author(s):  
Neeraj E Kolge ◽  
Vivek J Patni ◽  
Girish Karandikar ◽  
Ravindranath VK ◽  
Prateek Daga ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Frictional Resistance ◽  
Dimensional Accuracy ◽  
Surface Characteristics ◽  
Steel Wires ◽  
Group 5 ◽  
Group 2 ◽  
Deflection Rate ◽  
Group 1

Aims & Objectives: The aim of the study was to evaluate and compare dimensional accuracy, surface characteristics and load deflection attributes of SS wires sourced from six different commercial houses. Materials & Methods: 0.019” x 0.025” SS wire samples were divided into 6 groups and evaluated and compared for the aforementioned properties. Dimensional accuracy, surface roughness, frictional resistance, load deflection rate and ultimate tensile strength were evaluated and compared. Results: Dimensional accuracy did not vary significantly. Surface Profilometry proved 3M Unitek (Group 1) to be the wire with least irregularities. According to SEM, Modern Orthodontics (Group 6) and 3M Unitek (Group 1) in terms of ‘Sa’ and ‘Sq’ respectively had the smoothest surface finish. SS wires sourced from G&H Orthodontics (Group 2) exhibited least frictional resistance. Load deflection rates varied for all the groups for all three parameters under the study. UTS was best for SS wires sourced from Rabbit Force Orthodontics (Group 5). Conclusion: No wire from a single commercial house excels in all the parameters that were evaluated, each one having its aces and minuses.

Evaluation of physical and adhesive properties of enamel after a therapeutic dose of radiation and bonding of orthodontic metal brackets: an in vitro study

2020 ◽  
pp. 1-5
Author(s):  
A. Anushree ◽  
Ashutosh Shetty ◽  
Crystal Runa Soans ◽  
M. N. Kuttappa ◽  
Akhil Shetty ◽  
...  
Keyword(s):  
Testing Machine ◽  
In Vitro Study ◽  
Tooth Surface ◽  
Control Group ◽  
Adhesive Properties ◽  
Adhesive Resin ◽  
Adhesive Remnant Index ◽  
Group 3 ◽  
Group 2

Abstract Aim: The study aimed to evaluate the shear bond strength (SBS) of metal brackets and adhesive properties of bonded irradiated and non-irradiated teeth. Methods: Sixty-six extracted premolar samples were randomly divided into three groups—(a) Control group consisting of 22 non-irradiated, non-aged teeth (Group 1), (b) 22 non-irradiated, aged samples (Group 2) and (c) 22 irradiated, aged samples (Group 3). Irradiation was done using gamma irradiation with a fractionated dose of 60 Gy for 5 consecutive days per week over 6 weeks. Metal brackets were bonded on all samples with light cure adhesive and subjected to SBS test using universal testing machine. The samples were assessed under the scanning electron microscope to check for the adhesive remnant index (ARI) and tag depth. Results: There was a statistically significant decrease in the mean SBS of the irradiated samples compared to the non-irradiated teeth. The non-irradiated, aged samples showed a majority of ARI scoring 1 and 2. Whereas, the irradiated samples showed ARI scoring 2 and 3. Approximately, 77·3% of the non-irradiated samples showed no adhesive present on the tooth surface, and 27·2% of the irradiated samples had more than 50% adhesive present on the enamel surface. Conclusion: There is a statistically significant decrease in SBS of irradiated enamel compared to that of non-irradiated teeth. However, the SBS observed in the three groups was well above the ideal SBS for orthodontic bonding, that is, 5·6–6·8 MPa. The adhesive remnant was found on all samples of the irradiated group. Deeper adhesive resin tags were found in the irradiated group in the resin–enamel interface.

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