n. 188 (16): Dor On Line

Dor on line ◽  
2016 ◽  
Author(s):  
Paulo Barboni
Keyword(s):  
Urtica Dioica ◽  
On Line

Edição de Março de 2016 - Ano 16 - Número 188   Olá, leitores! Epigenética e dor é o tema de nosso editorial desse mês. Na seção de Divulgação Científica, trazemos alertas tratando de assuntos como o uso da pupilometria na avaliação da analgesia opioide; a relação do excesso de trabalho e a lombalgia; novas propostas de analgésicos, inclusive em serviços de música on line; e mais um artigo ligado ao “Ano de Combate à Dor Articular”. Na seção de Ciência e Tecnologia, trazemos alertas tratando da inibição da via da tetrahidrobiopterina; a identificação de um fator ligado à proliferação microglial em nervos lesionados; um alvo proteico ligado ao estresse e dor crônica e um novo tipo de priming hiperalgésico. Boa Leitura!   Editorial Participação de mecanismos epigenéticos na dor crônica: uma crescente área de investigação Andreza Urba de Quadros   Divulgação Científica Movimento da pupila pode predizer redução da dor pela administração de opioides. Pupilometria auxilia no tratamento da dor. Alexandre Hashimoto Pereira Lopes Dor nas costas aumenta em pessoas viciadas em trabalho. Você é um workaholic? Se sim, cuidado! Dênis Augusto Santana Reis Analgésicos potentes para o alívio da dor. Pesquisa mostra o efeito de análogos às endomorfinas administrados para aliviar a dor. Andressa Daiane de Carvalho Zaparolli Dorflex cria 'analgésico musical'. O uso de uma terapia musical pode funcional como um tratamento complementar ao tratamento farmacológico. Dênis Augusto Santana Reis Prescrição e efeito total da utilização de analgésicos, hipnóticos, antidepressivos e ansiolíticos. Resultado proveniente de uma população total de pacientes com artroplastia no quadril. Ana Carolina Alves M. de Moura   Ciência e Tecnologia Redução da dor inflamatória e neuropática através da inibição da via da tetrahidrobiopterina (BH4). Pesquisadores desenvolveram um inibidor da via de BH4 capaz de reduzir a dor crônica sem causar tolerância e efeitos adversos. Flávia Viana Santa-Ceclília CSF-1 derivado do nervo sensorial lesionado induz proliferação microglial e dor via dependente de DAP12. Neurônios sensoriais lesionados ativam a micróglia. Miriam das Dores Mendes Fonseca Avaliação in vivo e in vitro dos efeitos da Urtica dioica e natação em fatores do diabetes. A Urtiga é utilizada como planta medicinal para o tratamento de diversas patologias. Erika Ivanna Araya Pallarés Proteína FKBP51, envolvida na resposta do organismo ao estresse, envolvida na origem da dor crônica. Proteína FKBP51 como um possível alvo terapêutico para o tratamento da dor crônica. Sabrina Francesca de Souza Lisboa Agonista do receptor adenosina-a1 induz sensibilização hiperalgésica tipo II. Modelo de cronificação da dor. Thatiane Sandielen Lima Soares

scholarly journals Assessment of Conjugate Complexes of Chitosan and Urtica dioica or Equisetum arvense Extracts for the Control of Grapevine Trunk Pathogens

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 976
Author(s):  
Natalia Langa-Lomba ◽  
Laura Buzón-Durán ◽  
Pablo Martín-Ramos ◽  
José Casanova-Gascón ◽  
Jesús Martín-Gil ◽  
...  
Keyword(s):  
Urtica Dioica ◽  
Gas Chromatography Mass Spectrometry ◽  
Equisetum Arvense ◽  
Grapevine Trunk Diseases ◽  
Trunk Diseases ◽  
Synergistic Behavior ◽  
Reported Data ◽  
Basic Substances

In the work presented herein, we analyze the efficacy of three basic substances that comply with European Regulation (EC) No 1107/2009, namely chitosan, horsetail (Equisetum arvense L.) and nettle (Urtica dioica L.), for the control of grapevine trunk diseases (GTDs) in organic farming. The E. arvense and U. dioica aqueous extracts, prepared according to SANCO/12386/2013 and SANTE/11809/2016, have been studied by gas chromatography–mass spectrometry (GC-MS), identifying their main active constituents. The three basic substances, either alone or in combination (forming conjugate complexes), have been tested in vitro against eight Botryosphaeriaceae species, and in vivo, in grafted plants artificially inoculated with Neofusicoccum parvum and Diplodia seriata. A clear synergistic behavior between chitosan and the two plant extracts has been observed in the mycelial growth inhibition tests (resulting in EC90 values as low as 208 μg·mL−1 for some of the isolates), and statistically significant differences have been found in terms of vascular necroses lengths between treated and non-treated plants, providing further evidence of aforementioned synergism in the case of D. seriata. The reported data supports the possibility of extending the applications of these three basic substances in Viticulture beyond the treatment of mildew.

A Model for Non-Invasive Estimation of in Vivo Dialyzer Performances and Patient's Conductivity during Hemodialysis

1993 ◽  
Vol 16 (8) ◽  
pp. 585-591 ◽  
Author(s):  
T. Petitclerc ◽  
N. Goux ◽  
A.L. Reynier ◽  
B. Béné
Keyword(s):  
Strong Correlation ◽  
New Method ◽  
Conductivity Measurements ◽  
Urea Clearance ◽  
Non Invasive ◽  
Model Based ◽  
On Line ◽  
Conventional Methods

On-line monitoring of hemodialysis sessions requires a non-invasive estimation of the parameters concerning the patient's status and the dialyzer performances. We describe here a model based on a new method for non-invasive dialysance and patient conductivity measurements. In this technique the same probe measures alternately the conductivity at the dialysate inlet and outlet for two different dialysate conductivity values. From these data, an appropriate model allows to determine the patient's conductivity as well as the effective dialysance of ionised solutes, that is to say the dialysance corrected for recirculation. A strong correlation is evidenced between the effective dialysance measured by this method and the urea clearance measured by conventional methods (r=0.98 for in vitro solutions; r=0.82 in in vivo situations).

On-Line Hemodiafiltration with Pre- and Postdilution: A Comparison of Efficacy

1997 ◽  
Vol 20 (2) ◽  
pp. 81-90 ◽  
Author(s):  
P. Ahrenholz ◽  
R.E. Winkler ◽  
W. Ramlow ◽  
M. Tiess ◽  
W. Müller
Keyword(s):  
Molecular Weight ◽  
Theoretical Description ◽  
Convective Transport ◽  
Small Molecular Weight ◽  
Dialysate Flow ◽  
Significant Difference ◽  
On Line ◽  
The Impact

Since the introduction of on-line substituate preparation, high substituate rates (Qs) in pre- and postdilution for hemodiafiltration (HDF) procedures can be realized. During postdilution HDF (POD-HDF) and additional convective removal is possible, but in vivo Qs is limited to approx. 1/3Qb (bloodflow). With predilution HDF (PRD-HDF) higher Qs and therefore high convective transport rates by ultrafiltration can be reached. On the other hand the blood concentration is diminished by predilution. Further decrease of the diffusive transport is caused by reduced dialysate flow Qd due to separation of the substituate from the dialysate (Fresenius 4008 On-Line HDF, Gambro AK100 Ultra). The theoretical description of the combined diffusive-convective transport is limited to 1-dimensional models and small UF-rates. Therefore for practical and theoretical purposes the assessment of the efficacy of on-line PRD-HDF and POD-HDF in different molecular weight ranges is desirable. By means of in vitro experiments the effective clearances Keff of hemodialysis (HD, dialyzer: Fresenius F60) for urea, creatinine, vitamin B12 and inulin were compared with measured and theoretical Keff of POD- and PRD-HDF. The theoretical expectation is confirmed that Keff for small molecular weight substances decreases slightly with PRD-HDF and increases for larger molecules. In the case of POD-HDF Keff for small molecular weight substances increases slightly and strongly for larger molecules. In vivo experiments were performed to measure the real substance removal from patient's blood and to figure out the impact of dialysate flow (collection of the used dialysate during the 1. treatment hour and concentration measurements for urea, creatinine, phosphate, ß2-MG). The results show that the substraction of Qs from Qd reduces Keff for urea, creatinine and phosphate but not for ß2-MG. PRD-HDF with Qd = 500 ml/min is significantly less effective for small molecules than HD. There is no significant difference of Keff for urea, creatinine, phosphate during HD and PRD-HDF with Qd = 800 ml/min, but a significant increase of 10-15% for POD-HDF Keff for ß2-MG increases by 75% for PRD-HDF and 95% for POD-HDF compared with HD (Qd = 500 ml/min).

scholarly journals MYC-mediated upregulation of PNO1 promotes glioma tumorigenesis by activating THBS1/FAK/Akt signaling

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
Xu Chen ◽  
Zheng-Qian Guo ◽  
Dan Cao ◽  
Yong Chen ◽  
Jian Chen
Keyword(s):  
Cell Proliferation ◽  
Tissue Microarrays ◽  
Akt Signaling ◽  
Akt Pathway ◽  
Tumor Cell Growth ◽  
Tumor Promotor ◽  
On Line ◽  
Proliferation And Invasion

AbstractPNO1 has been reported to be involved in tumorigenesis, however, its role in glioma remains unexplored. In the present study, PNO1 expression in glioma from on-line databases, cDNA, and tissue microarrays was upregulated and associated with poor prognosis. PNO1 knockdown inhibits tumor cell growth and invasion both in vitro and in vivo; whereas PNO1 overexpression promoted cell proliferation and invasion in vitro. Notably, PNO1 interacted with THBS1 and the promotion of glioma by PNO1 overexpression could be attenuated or even reversed by simultaneously silencing THBS1. Functionally, PNO1 was involved in activation of FAK/Akt pathway. Moreover, overexpressing MYC increased PNO1 promoter activity. MYC knockdown decreased PNO1 and THBS1 expression, while inhibited cell proliferation and invasion. In conclusion, MYC-mediated upregulation of PNO1 contributes to glioma progression by activating THBS1/FAK/Akt signaling. PNO1 was reported to be a tumor promotor in the development and progression of glioma and may act as a candidate of therapeutic target in glioma treatment.

scholarly journals POKRZYWA ZWYCZAJNA (URTICA DIOICA L.) – CHARAKTERYSTYKA BOTANICZNA, BIOCHEMICZNA I WŁAŚCIWOŚCI PROZDROWOTNE

2016 ◽  
Vol 61 (2) ◽  
pp. 191 ◽  
Author(s):  
Karolina Jakubczyk ◽  
Katarzyna Janda ◽  
Sylwia Szkyrpan ◽  
Izabela Gutowska ◽  
Jolanta Wolska
Keyword(s):  
Urtica Dioica

Pokrzywa zwyczajna (Urtica dioica L.) należy do rodziny pokrzywowatych (Urticaceae). Rośnie w formie dzikiej w Azji, Europie, Ameryce Północnej i Afryce Północnej. Jako roślina ruderalna powszechnie znajduje się również w Polsce. Pokrzywa zwyczajna dzięki składowi bogatemu w związki biologicznie czynne uważana jest za jedną z najcenniejszych roślin stosowanych w fitoterapii. W modelach in vitro i in vivo wykazano jej właściwości antyoksydacyjne, przeciwzapalne, antyagregacyjne, hipoglikemiczne oraz hipocholesterolemiczne. Badania prowadzone w ostatnich latach wskazują na możliwość wykorzystania pokrzywy zwyczajnej w chemoprewencji, leczeniu cukrzycy, łagodnego rozrostu gruczołu krokowego oraz schorzeń urologicznych.

The Use of On-line and Off-line Chromatographic Extraction Techniques coupled with Mass Spectrometry for Support of In Vivo and In Vitro Assays in Drug Discovery

2001 ◽  
Vol 1 (5) ◽  
pp. 463-471 ◽  
Author(s):  
John A. Masucci ◽  
Gary W. Caldwell ◽  
William J. Jones ◽  
Stephen J. Juzwin ◽  
Patrick J. Sasso ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Discovery ◽  
In Vitro Assays ◽  
Extraction Techniques ◽  
On Line ◽  
Chromatographic Extraction

A Combined Heat Clearance Method for Tissue Blood Flow Measurement

1991 ◽  
Vol 113 (4) ◽  
pp. 438-445 ◽  
Author(s):  
Sarit Abramovich-Sivan ◽  
Vili Benary ◽  
Thalia Kaspi ◽  
Solange Akselrod
Keyword(s):  
Blood Flow ◽  
Pulse Method ◽  
Continuous Method ◽  
Tissue Blood Flow ◽  
Experimental Systems ◽  
In Vitro Measurements ◽  
On Line ◽  
Heat Clearance

Tissue Blood Flow is measured by applying a combined procedure of two independent approaches based on heat clearance: the Pulse Decay Method and the Continuous Method. The Pulse Method allows absolute assessment of tissue BF with no need for calibration, and can be applied only if the tissue BF is steady during the period of measurement. On the other hand, the Continuous Method enables the observation of rapid changes in tissue BF, and can be applied under non steady-state conditions. Using the combined method, a continuous quantitative measurement of transient changes in tissue BF can be obtained. For this purpose, we have developed two experimental systems consisting of independent electronic units: a Pulse Unit and a Continuous Unit. A micro-computer with dedicated software controls the operation of the electronic units and calculates tissue BF on-line. In vitro measurements are performed and demonstrate the reliability of the methods. In vivo measurements in rat brain tissue are also performed and include physiological and pharmacological changes of local tissue BF. The results of the two heat clearance methods correlate well with tissue BF values measured by a third independent method, the Hydrogen Clearance Method.

Cardiovascular effects of Urtica dioica L. (Urticaceae) roots extracts: in vitro and in vivo pharmacological studies

2002 ◽  
Vol 81 (1) ◽  
pp. 105-109 ◽  
Author(s):  
Lara Testai ◽  
Silvio Chericoni ◽  
Vincenzo Calderone ◽  
Giulia Nencioni ◽  
Paola Nieri ◽  
...  
Keyword(s):  
Cardiovascular Effects ◽  
Urtica Dioica ◽  
Pharmacological Studies

scholarly journals PNO1 promotes human cholangiocarcinoma tumorigenesis and chemosensitivity

2021 ◽  
Author(s):  
Chuang Chen ◽  
Shan Huang ◽  
Guobin Wu ◽  
Zongyu Wang ◽  
Dongyi Lin ◽  
...  
Keyword(s):  
Cell Viability ◽  
High Throughput Screening ◽  
Ribosome Biogenesis ◽  
Bortezomib Treatment ◽  
P53 Signaling ◽  
Global Protein Synthesis ◽  
On Line

Abstract Background The partner of NOB1 homolog (PNO1) is important for ribosome biogenesis and serves as an oncogene in several cancers. However, the role of PNO1 in cholangiocarcinoma (CHOL) remains largely unknown. Methods The method of mRNA microarray analysis, high-throughput screening technologies, on-line databases analysis, PDXs models, biochemistry and molecular biology have been utilized to reveal the role of PNO1 in the progression of CHOL. Results PNO1 was significantly upregulated in CHOL tissues and predicted poor prognosis. PNO1 could induce cell proliferation and metastasis in vitro and in vivo. In CHOL cells expressing wild-type p53, PNO1 knockdown increased expression of p53 and its downstream gene p21 and decreased cell viability; these effects were blocked by p53 knockout and attenuated by the p53 inhibitor PFT-a. Furthermore, PNO1 knockdown reduced global protein synthesis and inhibiting MDM2-mediated ubiquitination and p53 degradation. Moreover, PNO1 overexpression enhanced the sensitivity to bortezomib treatment in CHOL. In addition, MYC overexpression promoted PNO1 promoter activity while MYC knockdown decreased PNO1 mRNA and protein, which led to decreased cell viability and clone formation. The expression of MYC was found positively correlated with PNO1 in CHOL patients. Conclusions Collectively, upregulation of PNO1 by MYC promotes cholangiocarcinoma tumorigenesis through suppressing p53 signaling pathway and enhanced the sensitivity to bortezomib treatment. PNO1 serves as a candidate of therapeutic target in CHOL treatment and clinical chemotherapy regimen.

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