scholarly journals Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach

2021 ◽  
Vol 51 (3) ◽  
pp. 372-377
Author(s):  
Zeynep Doğan ◽  
Yasin Genç ◽  
Ümmühan Harput ◽  
Asuman Karadeniz Pekgöz ◽  
İclal Saraçoğlu
Keyword(s):  
Cytotoxic Activity ◽  
Aqueous Extract ◽  
Chemical Profiling

Cytotoxic activity of aqueous extract from Holodiscus discolor (Pursh) Maxim. leaves

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
M Mrižová ◽  
Ľ Laciková ◽  
M Haladová ◽  
E Eisenreichová ◽  
D Granèai ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Aqueous Extract

scholarly journals In vitro cytotoxic activity of aqueous extract of Delonix elata (L.) gamble (fabaceae) leaves on mcf-7 & hep g-2 cell lines

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Phani Kumar Kola ◽  
Shyam Prasad K ◽  
Lakshmi Sudeepthi N ◽  
R.CH.Sekhara Reddy D ◽  
Abdul Rahaman Sk ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Mcf 7

scholarly journals ­Chemical profiling and biological activity of Peperomia blanda (Jacq.) Kunth

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4839 ◽  
Author(s):  
Wafa M. Al-Madhagi ◽  
Najihah Mohd Hashim ◽  
Nasser A. Awad Ali ◽  
Abeer A. Alhadi ◽  
Siti Nadiah Abdul Halim ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Radical Scavenging ◽  
Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Standard Drug ◽  
Chemical Profiling ◽  
First Time ◽  
Mcf 7

Background Peperomia belongs to the family of Piperaceae. It has different uses in folk medicine and contains rare compounds that have led to increased interest in this genus. Peperomia blanda (Jacq.) Kunth is used as an injury disinfectant by Yemeni people. In addition, the majority of Yemen’s population still depend on the traditional remedy for serious diseases such as cancer, inflammation and infection. Currently, there is a deficiency of scientific evidence with regards to the medicinal plants from Yemen. Therefore, this study was performed to assess the chemical profile and in vitro antioxidant and cytotoxic activities of P. blanda. Methods Chemical profiling of P. blanda was carried out using gas chromatography mass spectrometry (GCMS) followed by isolation of bioactive compounds by column chromatography. DPPH• and FRAP assays were used to evaluate antioxidant activity and the MTT assay was performed to estimate the cytotoxicity activity against three cancer cell lines, namely MCF-7, HL-60 and WEHI-3, and three normal cell lines, MCF10A, WRL-68 and HDFa. Results X-ray crystallographic data for peperomin A is reported for the first time here and N,N′-diphenethyloxamide was isolated for the first time from Peperomia blanda. Methanol and dichloromethane extracts showed high radical scavenging activity with an IC50 of 36.81 ± 0.09 µg/mL, followed by the dichloromethane extract at 61.78 ± 0.02 µg/mL, whereas the weak ferric reducing activity of P. blanda extracts ranging from 162.2 ± 0.80 to 381.5 ± 1.31 µg/mL were recorded. In addition, petroleum ether crude extract exhibited the highest cytotoxic activity against all the tested cancer cell lines with IC50 values of 9.54 ± 0.30, 4.30 ± 0.90 and 5.39 ± 0.34 µg/mL, respectively. Peperomin A and the isolated mixture of phytosterol (stigmasterol and β-sitosterol) exhibited cytotoxic activity against MCF-7 and WE-HI cell lines with an IC50 of (5.58 ± 0.47, 4.62 ± 0.03 µg/mL) and (8.94 ± 0.05, 9.84 ± 0.61 µg/mL), respectively, compared to a standard drug, taxol, that has IC50 values of 3.56 ± 0.34 and 1.90 ± 0.9 µg/mL, respectively. Conclusion The activities of P. blanda extracts and isolated compounds recorded in this study underlines the potential that makes this plant a valuable source for further study on anticancer and antioxidant activities.

scholarly journals Erythrocyte Osmotic Fragility and Cytotoxic Activity of the Aqueous Extract of Leaves of Indigofera Suffruticosa Mill (Fabaceae)

2019 ◽  
Vol 11 (6) ◽  
pp. 1802-1809
Author(s):  
Tainá M. S. da Silva ◽  
Renatha C. B. Sobreira ◽  
Jéssica de A. G. Silva ◽  
Marcos A. S. da Costa ◽  
Maria I. de A. Lima ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Aqueous Extract ◽  
Osmotic Fragility ◽  
Erythrocyte Osmotic Fragility

scholarly journals UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Hanan M. Al-Yousef ◽  
Wafaa H. B. Hassan ◽  
Sahar Abdelaziz ◽  
Musarat Amina ◽  
Rasha Adel ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Aqueous Extract ◽  
Biological Activities ◽  
Biological Effects ◽  
Aqueous Extracts ◽  
Potent Effect ◽  
Esi Ms ◽  
Biological Studies ◽  
Hct 116

The aqueous extracts of Hibiscus calyphyllus (HcA), Hibiscus micranthus (HmA), and Hibiscus deflersii (HdA) growing in Saudi Arabia did not receive enough attention in phytochemical and biological studies. This inspired the authors to investigate the phytochemicals of these extracts for the first time using UPLC-ESI-MS/MS in negative and positive ionization modes. The analysis afforded the tentative identification of 103 compounds including phenolic compounds, flavonoids, and anthocyanins. Moreover, in vitro evaluations of their cytotoxic, antioxidant, antidiabetic, and antiobesity activities were carried out. The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. Hibiscus deflersii showed the most potent cytotoxic effect against A-549 (human lung carcinoma) with IC50 = 50 ± 5.1 μg/mL, and Hibiscus micranthus showed a close effect with IC50 = 60.4 ± 1.7 μg/mL. Hibiscus micranthus showed the most potent effect on HCT-116 (human colon carcinoma) with IC50 = 56 ± 1.9 μg/mL compared with cisplatin (IC50 = 7.53 ± 3.8 μg/mL). HcA and HdA extracts showed weak cytotoxic activity against A-549 and HCT-116 cell lines compared to the other extracts. Eventually, Hibiscus deflersii showed astonishing antidiabetic (IC50 = 56 ± 1.9 μg/mL) and antiobesity (IC50 = 95.45 ± 1.9 μg/mL) activities using in vitro α-amylase inhibitory assay (compared with acarbose (IC50 = 34.71 ± 0.7 μg/mL)) and pancreatic lipase inhibitory assay (compared with orlistat (IC50 = 23.8 ± 0.7 μg/mL)), respectively. In conclusion, these findings are regarded as the first vision of the phytochemical constituents and biological activities of different Hibiscus aqueous extracts. Hibiscus deflersii aqueous extract might be a hopeful origin of functional constituents with anticancer (on A-549 cell line), antidiabetic, and antiobesity activities. It might be a natural alternative remedy and nutritional policy for diabetes and obesity treatment without negative side effects. Isolation of the bioactive phytochemicals from the aqueous extracts of aerial parts of Hibiscus calyphyllus, Hibiscus micranthus, and Hibiscus deflersii and estimation of their biological effects are recommended in further studies.

scholarly journals Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study

2015 ◽  
Vol 16 (9) ◽  
pp. 709-714 ◽  
Author(s):  
Prachi Shivpuje ◽  
Renuka Ammanangi ◽  
Kishore Bhat ◽  
Sandeep Katti
Keyword(s):  
Oral Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Aqueous Extract ◽  
Cancer Cell Line ◽  
Ocimum Sanctum ◽  
Oral Cancer Cell ◽  
Oral Cancer Cell Line

ABSTRACT Background Cancer till today remains the leading cause of death in both developed and developing countries. Plants have been beacon of therapeutic sources for curing diseases from times immemorial. Hence, the present study aimed at evaluating the antiproliferative activity of extract of Ocimum sanctum leaves on oral cancer cell line. Objectives: • To evaluate the antiproliferative effect and to analyze dose dependent cytotoxic activity of aqueous extract of O. sanctum leaves on KB mouth cell line. • To compare the effectiveness among different variety of O. sanctum. Materials and methods KB cells (Mouth Epidermal Carcinoma Cells) were used for the present study. Aqueous and dry extract of O. sanctum with both dark (Krishna Tulsi) and light (Rama Tulsi) leaves were prepared in the institution. The antiproliferative and cytotoxic activity on KB cell line was evaluated by MTT assay. Statistical analysis with Mann-Whitney U-test and Wilcoxon matched pairs test was carried out. Results The aqueous extract of O. sanctum of both the leaves exhibited significant cytotoxic effect against oral cancer cell line. Conclusion Aqueous extract of O. sanctum leaves was effective as an antiproliferative agent which caused apoptosis in oral cancer cell line. Clinical significance Ocimum sanctum herb which is abundantly grown in India can be used for its anticancer properties for treating oral cancer. This will not only be cost-effective but will also have less or no side effects. How to cite this article Shivpuje P, Ammanangi R, Bhat K, Katti S. Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study. J Contemp Dent Pract 2015;16(9):709-714.

scholarly journals Interferon-γ-Mediated Natural Killer Cell Activation by an AqueousPanax ginsengExtract

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Kazuyoshi Takeda ◽  
Ko Okumura
Keyword(s):  
Cytotoxic Activity ◽  
Nk Cells ◽  
Natural Killer ◽  
Aqueous Extract ◽  
Cell Activation ◽  
Mononuclear Cells ◽  
Nk Cell ◽  
Killer Cell ◽  
Cell Activity

Panax ginsengextracts are used in traditional herbal medicines, particularly in eastern Asia, but their effect on natural killer (NK) cell activity is not completely understood. This study aimed to examine the effects ofP. ginsengextracts on the cytotoxic activity of NK cells. We orally administeredP. ginsengextracts or ginsenosides to wild-type (WT) C57BL/6 (B6) and BALB/c mice and to B6 mice deficient in either recombination activating gene 2 (RAG-2) or interferon-γ(IFN-γ). We then tested the cytotoxic activity of NK cells (of spleen and liver mononuclear cells) against NK-sensitive YAC-1 cells. Oral administration ofP. ginsengaqueous extract augmented the cytotoxicity of NK cells in WT B6 and BALB/c mice and in RAG-2-deficient B6 mice, but not in IFN-γ-deficient B6 mice. This effect was only observed with the aqueous extract ofP. ginseng. Interestingly, the ginsenosides Rb1 and Rg1 did not augment NK cell cytotoxicity. These results demonstrated that the aqueousP. ginsengextract augmented NK cell activationin vivovia an IFN-γ-dependent pathway.

scholarly journals Evaluation of Antibacterial, Anti-Oxidant and Cytotoxic Activity of Organic Extracts of Mahogany Seeds

2020 ◽  
pp. 1-7
Author(s):  
. Assaduzzaman ◽  
Md. Ziaul Amin ◽  
Mohammad Habibur Rahman ◽  
Md. Rasel Uddin ◽  
Md. Shohanuzzaman ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Aqueous Extract ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Aqueous Extracts ◽  
Brine Shrimp Lethality ◽  
Oxidative Stresses ◽  
Organic Extracts ◽  
Anti Oxidant

The perception of normal medicine is changing, and the use of traditional or herbal medicine increasing worldwide due to our harmonious nature of the biological system. Many parts of the plants possess an impressive array of medicinal benefits, mahogany seed is one of them. The aim of this study is to evaluate the antimicrobial, anti-oxidant and cytotoxic activity of organic extracts of mahogany seeds. For determination of antimicrobial and cytotoxic activity disk diffusion and brine shrimp lethality bioassay were used. DPPH free radical test were performed for evaluation of antioxidant activity. Ethanol, n-hexane and aqueous extracts were examined with the total number of 10 bacterial strain. Here, ethanol extract showed higher antimicrobial activity than n-hexane and aqueous extract for both gram positive and negative bacteria. In brine shrimp lethality bioassay LC50 values were 82 μg/ml, 95 μg/ml and 93 μg/ml for ethanol, n-hexane and aqueous extracts respectively. The scavenging DPPH free radicals were found to be concentration dependent. The LC50 value was measured at 140µg/ml, 160µg/ml and 180µg/ml for ethanol, n-hexane and aqueous extract. The results of this study indicated that the mahogany seeds can be used as an easily accessible source of natural antioxidants and Cytotoxic compounds which might be helpful in preventing the progress of various oxidative stresses and cell culture assay.

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