scholarly journals Effect of Moringa leave Marinade on aflatoxin in fresh and smoked African catfish (Clarias gariepinus)

Food Research ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 185-194
Author(s):  
E.O. Oni ◽  
M.C. Adetunji ◽  
D.D. John-Babatunde ◽  
A.M. Omemu
Keyword(s):  
Clarias Gariepinus ◽  
Storage Period ◽  
Aflatoxin Contamination ◽  
African Catfish ◽  
Fresh Fish ◽  
Smoked Fish ◽  
Fish Samples ◽  
Aflatoxin Concentration ◽  
Bacteria And Fungi ◽  
And Control

This study aimed at evaluating the effect of Moringa leaves Marinade (MOM) on aflatoxin contamination of Clarias gariepinus. A total of thirty fresh fish samples (n = 30) grouped into four; Fresh fish without smoking and storage, smoked fish +1% and 2% Moringa oleifera Marinade (MOM) respectively and Control (0% MOM) were subjected to microbiological and HPLC analysis while storing for 0-3 months. Mean CFU/g of 2.8 and 3.1 x 102 CFU/g for bacteria and fungi were recovered, respectively. Furthermore, four bacteria and fungi genera each of which Aspergillus spp. was the most predominant (57%) were recovered from the fishes. Aflatoxin concentration increased progressively in 0% MOM smoked fish as the storage period increased, while aflatoxin concentration reduced in the fishes treated with 2% MOM (p≥0.05). On average, between 1-40% reduction in aflatoxin concentration and increased keeping quality was enhanced with 2% MOM treatment. These findings recommend the possibility of the use of moringa leaves in the treatment of commercially smoked fish.

scholarly journals Effect of Natural Preservatives (Lemon Grass and Bay Leaf Marinade) on the Microbial Load and Shelf Life of Smoke-dried Clarias gariepinus

2019 ◽  
pp. 1-10
Author(s):  
O. G. Benjamin ◽  
K. Omoruyi ◽  
A. E. Nneli
Keyword(s):  
Clarias Gariepinus ◽  
Microbial Load ◽  
Sample Treatment ◽  
Fish Sample ◽  
Fresh Fish ◽  
Lemon Grass ◽  
Ambient Storage ◽  
Fish Samples ◽  
Bacteria And Fungi ◽  
Natural Preservatives

This study was carried out to determine the effect of lemon grass marinade (sample A), bay leaf marinade (sample B) and a combination of both spices (sample C) on the microbial load of stored smoked-dried Clarias gariepinus. Comparative analysis of the microbial load of each treated fish samples during ambient storage was done biweekly for six (6) weeks and then samples of each treatment were taken to the laboratory periodically. The fish samples were analysed using Potato Dextrose Agar and Nutrient Agar for fungi and bacteria respectively. The initial microbial load before storage showed that the highest number of bacteria counts occurred in smoke-dried Clarias gariepinus treated with bay leaf marinade (4.7´103) while the lowest was in smoke-dried C. gariepinus treated with lemon grass marinade (1.3´103). The highest fungi count was observed in smoke-dried C. gariepinus treated with the combination of both spices (9.5´102) while the least fungi count was in fresh fish sample (1.4´103), although the fresh fish sample had the highest bacteria mean count (5.1´103) when compared with the treated samples. After 6 weeks of ambient storage, the result of smoke-dried C. gariepinus treated with bay leaf marinade was also significantly different from the mean population of smoke-dried C. gariepinus treated with lemon grass marinade and smoke-dried C. gariepinus treated with the combination of both spices. Smoke-dried C. gariepinus treated with bay leaf marinade again had the highest bacteria and fungi mean count of 1.7´105 and 2.3´104 respectively. Smoke-dried C. gariepinus treated with lemon grass marinade and smoke-dried C. gariepinus treated with the combination of both spices had similar bacteria mean count of 1.5´105 while smoke-dried C. gariepinus treated with lemon grass marinade had the lowest fungi mean count of 1.6´104. The study revealed that all three smoked-dried fish sample treatment had a relatively low bacterial and fungal count below the 5 x 105cfu/g recommended by the International Commission of Microbial Specification for Food and Food Products (ICMS, 2002).

scholarly journals Smoked African Catfish (Clarias gariepinus) Sampled from Two Markets and Occurrence of Microbes

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
John Abiodun Daramola ◽  
C. T. Kester ◽  
O. O. Allo
Keyword(s):  
Clarias Gariepinus ◽  
Storage Conditions ◽  
Plate Count ◽  
African Catfish ◽  
Smoked Fish ◽  
Ogun State ◽  
Ambient Storage ◽  
Fish Samples ◽  
Microbiological Parameters ◽  
Study Sites

The study was carried out to assess some microbiological qualities of smoked African catfish, Clarias gariepinus retailed in Sango and Ota markets in Ogun state, Nigeria. Six smoked fish retailers were randomly selected from the markets and 36 fish samples were collected. The samples of smoked catfish were assessed weekly for a period of six weeks for microbiological parameters such as Total plate count (TPC), Total coliforms count (TCC), Staphylococcus count and mould count. In this study, the microbial load of the smoked Clarias gariepinus samples retailed in the two study sites increased weekly during the period of storage. TPC increased from 4.75±0.014 log10 CFU/g for smoked samples from Ota market, retailer 1 (OMR1) in week 0 to 5.11 ±0.021 log10 CFU/g for samples from Ota market, retailer 3 (OMR3) in week 5.  TCC ranged from 1.47±0.001 log10 CFU/g to 2.43±0.049 log10 CFU/g; Staphylococcus from 2.15±0.070 log10 (CFU/g) to 2.66±0.014 log10 (CFU/g) and 1.82 ±0.070 log10 CFU/g to 2.92±0.071 log10 CFU/g for mould counts. The smoked Clarias gariepinus from the markets could not keep well till the 6th week. Hence, it can be inferred that smoked catfish with moisture content of about 10% or less will have a shelf life of about 4 - 5 weeks under ambient storage conditions before the effect of spoilage microorganisms.

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

Impact of Ogun River pollution on antioxidants and proximate composition of African catfish (Clarias gariepinus)

2019 ◽  
Vol 46 (3) ◽  
pp. 163-184
Author(s):  
B. O. Onunkwor ◽  
T. O. Akinpelu ◽  
O. J. Oladipupo ◽  
R. N. Ugbaja ◽  
O. J. Olaoye ◽  
...  
Keyword(s):  
Proximate Composition ◽  
Clarias Gariepinus ◽  
Chemical Properties ◽  
Oxygen Demand ◽  
Control Fish ◽  
Average Weight ◽  
African Catfish ◽  
Fish Samples ◽  
The Impact ◽  
Ogun River

Most rivers in Nigeria are polluted without conscious efforts to abate ensuant consequences. Thus, Clarias gariepinus, an aquatic commercial food from Ogun River, Nigeria, was studied to investigate the impact of pollution on antioxidants and proximate composition. During the rainy season, 50 C. gariepinus (average weight 426.53 ± 24.40g and length 37.73 ± 0.95cm) were site sampled, 10 from each of Ago-Odo, Ago-Ika, Oke-Sokori, and Quarry along Ogun River course. Ajegunle-Adao cultured pond, served as control. The parameters assayed for included: some heavy metals and physico-chemical properties of sampled water, antioxidants and proximate composition of the fish samples. Physicochemical parameters of river studied showed that temperature of all sites ranged from 26 to 29°C, pH: 6.9 to 7.8, dissolved oxygen: 9.5 to 21.6, biochemical oxygen demand: 3.60 to 14.10, and chemical oxygen demand: 60 to 650. Lead (Pb) concentration ranged from 4.0 to 17.0 μg/L and cadmium (Cd): 0.0 to 6.0 μg/L. Blood Pb, Cd, activities of: superoxide dismutase, catalase, glutathione-S-transferase, plasma xanthine oxidase, and lipid peroxidation level of fish samples from Ago-Ika were most significantly (p < 0.05) high, with significant (p < 0.05) lowest reduced glutathione concentration amongst the samples from polluted sites when compared to control fish. Proximate analysis showed that the fish samples from Ogun River had significantly (p < 0.05) lower protein, fat contents, and higher nitrogen-free extract while Ago-Ika, Oke-Sokori and Quarry sites had significantly (p < 0.05) lower moisture, ash and fibre contents when compared with control. It can be concluded that the impact of pollution induced oxidative stress and significantly diminished the nutritional value of C. gariepinus from Ogun River.

Description of Freshwater Fish Traditional Smoking in the Western Region, Cameroon

2021 ◽  
Vol 14 (1) ◽  
pp. 61-64
Author(s):  
Felix Meutchieye ◽  
Henri Grisseur Djoukeng ◽  
Youssouf Jamilou Ngouyamsa ◽  
Yacouba Manjeli
Keyword(s):  
Ethnic Group ◽  
Freshwater Fish ◽  
Oreochromis Niloticus ◽  
Clarias Gariepinus ◽  
Small Scale ◽  
African Catfish ◽  
Economic Activities ◽  
Fresh Fish ◽  
Hygiene Measures ◽  
Average Quantity

Western Cameroon is one of the regions most in needs of innovative and healthy solutions for freshwater fish conservation. This study aimed at evaluating the socio-economical characteristics of fish smokers and technical factors on the species smoked in the Noun Division. A total of 91 fish smokers were chosen. The socio-economic data were on age, gender, religion, number of dependents, marital status, ethnic group, education level, economic activities, smoking goals and workforce. The results of this study showed that fish smoking is practiced mainly by women (70%) which is common in Sub Sahara Africa small scale fish sector. The smokers are aged from 20 to 50 years (80%), married (92.2%) and taking care of a family of 1 to 10 persons (80%). These smokers are Muslim (74%), belonging to the Bamoun ethnic group (81%). They were also involved in other activities: agriculture (29%) and trade (1%). About 67% of the sampled smokers’ population had an experience of more than 10 years in the domain. The Purpose of smoking was largely sales (97%) and subsistence (3%). Nile tilapia (Oreochromis niloticus) was the most smoked species (36%), followed by African catfish (Clarias gariepinus) with a proportion of 32%. The average quantity of fresh fish smoked was 110 kg/day/smoker, with Oreochromis niloticus being more produced (60±4.74 kg/day/smoker). With regard to the smoking techniques used, hygiene measures were poorly respected. The major constraints revealed by the survey were that freshwater’s traditional fish smoking activities in Noun Division are influenced by gender, economic and cultural background. There is a lack of technical support material and financial means. Any implications for future investigations on health and food safety will be suitable for fresh fish smokers and the entire consumers.

scholarly journals Growth rate of african catfish (Clarias gariepinus) and plankton diversity in ponds under organic and inorganic fertilization

2021 ◽  
Vol 21 (02) ◽  
pp. 17545-17559
Author(s):  
PA Otieno ◽  
◽  
DO Owiti ◽  
PO Onyango ◽  
◽  
...  
Keyword(s):  
Growth Rate ◽  
Clarias Gariepinus ◽  
Chicken Manure ◽  
Average Weight ◽  
African Catfish ◽  
Phytoplankton Species ◽  
Inorganic Fertilizers ◽  
Average Growth ◽  
Plankton Diversity ◽  
And Control

Aquaculture offers the opportunity for safeguarding local and global food security in the face of declining capture fisheries. However, the form of aquaculture that is commonly practiced in Kenya is characterized by the use of agrochemicals such as fertilizers that negatively impact biodiversity especially when effluents from fish ponds drain into water bodies. This study aimed to determine differences in the growth rate of Clarias gariepinus, an important aquaculture fish in Kenya, to assess plankton diversity, and to identify phytoplankton species associated with pollution under organic and inorganic fertilization regimens using chicken manure, Diammonium phosphate, and urea, respectively. Average growth rate calculated per day was higher in the organically-fertilized ponds at 0.06 cm/day, followed by inorganically-fertilized ponds at 0.05cm/day and then, the control at 0.04 cm/day. Average weight gain was higher in organically-fertilized ponds at 0.08 g/day followed by ponds fertilized with inorganic fertilizer at 0.07 g/day and the control, at 0.06g/day. There were significant differences in growth rate across fertilization regimens (length: F2, 264= 24.06, p = 0.0399; weight: F2, 264 = 20.89, p = 0. 0457). Specifically, although differences in growth rate of fish in organically and inorganically fertilized ponds were not significant, fish in fertilized ponds were on average, longer and weighed more than those in the control pond. Jaccard’s similarity index for phytoplankton was highest (0.38) between organically-fertilized ponds and control but lowest (0.25) between inorganically-fertilized ponds and control. Use of chicken manure produced the highest diversity of zooplankton (Shannon-Weiner’s H in organically-fertilized pond = 1.886; inorganic = 1.044, and control = 0.935). The use of DAP and urea produced the highest proportion of phytoplankton species associated with pollution. These results do not support the commonly reported notion that ponds fertilized using inorganic fertilizers are more productive. Findings suggest that the use of inorganic fertilizers may threaten biodiversity in aquatic ecosystems through the production of toxic algae.

scholarly journals Effect of smoking, drying and the combination of smoking-drying on the nutritional and sensory attributes of catfish (Clarias gariepinus)

2018 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
A. O Akinsola ◽  
O. A Idowu ◽  
G.O Akanbi ◽  
M. A Taiwo-Oshin
Keyword(s):  
Clarias Gariepinus ◽  
Sensory Attributes ◽  
Plate Count ◽  
Crude Fibre ◽  
Fresh Fish ◽  
Smoked Fish ◽  
Total Plate Count ◽  
Significant Difference ◽  
Post Harvesting ◽  
Proximate Analyses

Poor post harvesting technology such as handling, preservation and processing can lead to an unhealthy situation through massive spoilage and waste. This study assessed the effect of smoking, drying and the combination of smoking-drying on the nutritional and sensory attributes of catfish. Six kilograms of average fresh catfish were washed, eviscerated, drained, and divided into four portions of 1.5 kg each. Sample A served as control (fresh fish), while samples B, C, D were dried, smoked, dried and smoked fish respectively. Samples were analyzed using standard methods. The results of proximate analyses of samples showed that the moisture content ranged from 20.1 to 75.0 %, the protein content from 21.7 to 63.0 %, the fat content from 0.5 to 8.6 %, while the crude fibre ranged from 0.0 to 0.04 %. The total ash ranged from 1.1 to 4.9 %, while Carbohydrate (NFE) ranged from 0.1 to 3.5 %. Significant difference (p>0.05) was observed in the free fatty acids and pH of sample C compared to other samples. The total plate count ranged from 2.4 to 4.3x10-5 CFU/g. The sensory attributes result showed that sample D was the most preferred and acceptable. The study showed that dried-smoked fish had a better quality and was more preferred for consumption than singly dried or smoked catfish

Histamine in Fish Products Randomly Collected in Southern Italy: A 6-Year Study

2020 ◽  
Vol 83 (2) ◽  
pp. 241-248 ◽  
Author(s):  
ANTONELLO CICERO ◽  
GAETANO CAMMILLERI ◽  
FRANCESCO GIUSEPPE GALLUZZO ◽  
ILARIA CALABRESE ◽  
ANDREA PULVIRENTI ◽  
...  
Keyword(s):  
Southern Italy ◽  
Street Vendors ◽  
Mean Values ◽  
Fresh Fish ◽  
Smoked Fish ◽  
Canned Tuna ◽  
Fish Samples ◽  
Fish Product ◽  
Array Detection ◽  
Ultrahigh Performance Liquid Chromatography

ABSTRACT In total, 4,615 fresh and processed fish samples collected from 2010 to 2015 were analyzed for histamine by ultrahigh-performance liquid chromatography with diode array detection. Histamine levels were detected in 352 (7.6%) samples, with a maximum of 4,110 mg kg−1 and mean values of 908.9 ± 1,226.79 and 344.01 ± 451.18 mg kg−1 for fresh and processed fish samples, respectively. No histamine levels were found in canned tuna and smoked fish samples in contrast to most of the data reported in the literature. A low percentage (2.79%) of noncompliant samples was found. The highest mean values were found during 2011 and 2015 for fresh and processed fish samples, respectively, showing a significant (P &lt; 0.05) difference between the sampling years. The histamine contents found in fresh fish samples were significantly higher (P &lt; 0.05) than those of processed samples. Most of the positive samples came from street vendors, suggesting the need to improve inspection measures in these commercial categories to ensure fish product safety. HIGHLIGHTS

Effects of pre-treatment with extracts of Ocimum gratissimum and Vermonia amygdalina on proximate and organoleptic properties of processed Clarias gariepinus (Burchell, 1822)

2020 ◽  
Vol 35 (1) ◽  
pp. 39-46
Author(s):  
S.A. Ojukannaiye ◽  
L.A. Agbabiaka ◽  
I.I. Adedokun
Keyword(s):  
Clarias Gariepinus ◽  
Storage Period ◽  
Live Weight ◽  
Herbal Extract ◽  
Vernonia Amygdalina ◽  
Organoleptic Properties ◽  
Leaf Extracts ◽  
Mean Values ◽  
Ocimum Gratissimum ◽  
Fish Samples

The study evaluated nutritional and organoleptic properties of Clarias gariepinus pre-treated with aqueous leave extracts of Ocimum gratissimum and Vernonia amygdalina before processing. Thirty-six (36) C. gariepinus (live weight = 1.2± 0.2kg) samples were procured and processed for the study. Fish samples were divided into three parts of twelve fish each. Each part was grouped into three treatments having four fish in each group using a completely randomized design. Treatment one (T1) had fish immersed in 5% brine without herbal extract (control). In Treatment two (T2), fish were soaked in mixture of 5% brine and 5% O. gratissium (scent leaf) extracts while Treatment three (T3) had fish soaked in mixture of 5% brine and 5% V. amygdalina (bitter leaf) extracts all for 30 minutes. Treated fish samples were smoke-dried using charcoal kiln for 5 hours at temperature of 80 – 90° C. Samples were then cooled under ambient condition (30+3oC) for 4 hours. The smoke-dried fish samples were labeled according to treatments and stored for 7 days under aseptic condition. Organoleptic properties of the samples were conducted at every 24-hour of storage using a 9-point hedonic scale. Mean values of proximate composition of samples were significantly different (p<0.05). Protein content (70.01%) in T3 was higher than 68.31% and 66.62% in T2 and T1 respectively. Similarly, fat (5.12%) and ash (4.68%) levels for T3 were higher than 4.85% and 4.46% fat with 4.36% and 3.98% ash contents for T2 and T1 respectively. Results of mean scores for each organoleptic property decreased significantly (p<0.05) during storage period with no consistent trend in score pattern. Keywords: Clarias gariepinus, extract pretreatment, Ocimum gratissimum, organoleptic, Vernonia amygdalina.

scholarly journals Microbial quality of fish along with the Tilapia, African catfish and Sardinella artisanal value chains in Kpong and James Town, Ghana

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
EURYDICE ABOAGYE ◽  
KWAKU TANO-DEBRAH ◽  
ANGELA PARRY-HANSON KUNADU
Keyword(s):  
Food Safety ◽  
Value Chain ◽  
Value Chains ◽  
African Catfish ◽  
Fresh Fish ◽  
Key Stakeholders ◽  
Fish Samples ◽  
Handling Practices ◽  
Safety Knowledge

Abstract. Aboagye E, Tano-Debrah K, Kunadu APH. 2018. Microbial quality of fish along with the Tilapia, African catfish and Sardinella artisanal value chains in Kpong and James Town, Ghana. Bonorowo Wetlands 10: 1-17. Fish from artisanal sources constitute the most critical animal protein in the Ghanaian diet. The availability and safety of fish on the Ghanaian market is, however now unpredictable owing to potential rapid microbial growth which results from high ambient temperatures and poor handling along the artisanal value chains. Little is know about the small-scale fish value chains as well as the food safety knowledge and processing practices of key stakeholders involved. This study aimed at mapping out the artisanal fish value chains of Tilapia (Oreochromis niloticus), African catfish (Clarias gariepinus) and sardinellas (Sardinella aurita), and assessing the food safety knowledge and handling practices of key stakeholders along the selected value chains. A survey using semi-structured questionnaires and involving 93 fishers, 40 retailers, 40 processors, and 120 consumers was carried out to investigate stakeholders’ knowledge and practices of food safety along the value chain. Samples of the selected fish species were taken along their respective value chains to test for the presence of safety indicators (Salmonella, Vibrio, and Listeria species), hygiene indicators (Staphylococcus aureus and Escherichia coli), and spoilage organisms (Pseudomonas spp. and Proteus spp.). The mean scores for food safety of retailers, processors, and consumers were found to be generally insufficient at 55%, 43%, and 67.3% respectively. The stakeholders also scored poorly in their handling practices with mean scores of 41.2%, 63.0% and 58.6% for fishers, processors, and consumers, respectively. Estimated fish losses were highest at the retailer and consumer stages of the value chain with reported injuries as high as 35 to 100%. Pathogens such as Clostridium perfringens, enteropathogenic Escherichia coli, Staphylococcus aureus, Listeria spp. And Aeromonas sobria were isolated from fresh and on processed ready-to-eat fish samples. Salmonella spp. and Vibrio spp. not detected on any of the samples tested. Mesophilic counts in the range of 7.96 ± 0.68 to 2.95 ± 0.23 log cfu/g reported from fresh fish samples, with similarly high fecal coliform counts averaging 3.11 log cfu/g. Processed fish samples had average total counts, fecal coliform counts, and yeasts and mold counts of 3.11, 2.27, and 2.45 log cfu/g respectively. Proteus vulgaris and Proteus mirabilis were the predominant spoilage organisms present in almost all the fresh fish samples. This study provided much-needed insight into the unsatisfactory safety and quality of artisanal fish on the Ghanaian market and the specific microorganisms associated with them along the value chain. It also established the link between the food safety knowledge and handling practices of stakeholders within the value chain, and the actual quality and safety of fish on the market.

Sign in / Sign up

Export Citation Format

Share Document