scholarly journals Experimental Estimation of Embryotoxic Risks of Cadmium Chloride with Isolated Administration and in Combination with Salts of Citrate and Nanocomposite on its Basis

2021 ◽  
Vol 6 (4) ◽  
pp. 179-185
Author(s):  
I. I. Kolosova ◽  
◽  
E. G. Slesarenko ◽  
Yu. P. Myasoyed ◽  
R. V. Kovtunenko ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Cadmium Chloride ◽  
Young Female ◽  
Embryonic Mortality ◽  
Combined Effect ◽  
Control Group ◽  
Intragastric Administration ◽  
Female Wistar Rats ◽  
Negative Effect ◽  
Living Organisms

Cadmium compounds that enter living organisms pose the potential for serious health effects as they exhibit mutagenic, carcinogenic, gonadotoxic, teratogenic, allergenic and embryotoxic effects. It is important to study the mechanisms of their toxic effect on embryonic development and structural and functional formation of organs of living organisms and to search for their bioantogonists. The purpose of the study was to highlight the results of intragastric administration of cadmium chloride (1.0 mg/kg) with isolated administration and in combination with metal citrates (cerium, germanium, zinc, and nanocomposite (iodine + sulfur + selenium citrates)) on the overall course of rat embryogenesis. Materials and methods. For the study, young female Wistar rats weighing 170-200 g were used. They were divided into 6 groups, depending on the test substances, which they received from the 1st to the 19th day of gestation. Results and discussion. The analysis of the results of the experimental study showed the negative effect of cadmium chloride on the analyzed indicators of embryogenesis (preimplantation mortality, postimplantation mortality, total embryonic mortality), indicators of intrauterine survival, morphological (anatomical) malformations, as well as general delay in fetal development), both on the 13th and 20th days of pregnancy relative to the control group. In particular, the indicators of total embryonic mortality significantly increased by 4.2 times (13th day) and 3.7 times (20th day), with significant increase preimplantation mortality by 6.5 times, postimplantation mortality by 3.0 times on the 13th day of embryonic development, and on the 20th day, the preimplantation mortality indicator was by 14.0 times higher (p <0.01), and the postimplantation mortality indicator was insignificantly by 2.5 times higher than the control group. In the groups of the combined effect of cadmium chloride with metal citrates, there was a proven decrease in the indicators of total embryonic mortality (18.18% - 38.10%), preimplantation mortality (21.43% - 53.85%), postimplantation mortality (20.0% - 66.7%), an increase in the number of fetuses per female (12.66% -36.0%). Conclusion. In groups of the combined effect of cadmium chloride with metal citrates, the obtained data indicate a decrease in the accumulation of cadmium under the influence of the studied citrates, which allows them to be considered as potential bioantagonists of cadmium chloride

scholarly journals Change of Embryotoxic Effects of Metal Citrates Depending on the Duration of Their Introduction

2021 ◽  
Vol 6 (6) ◽  
pp. 259-266
Author(s):  
I. I. Kolosova ◽  
◽  
L. Yu. Bogomolna ◽  
G. Yu. Chriss ◽  
N. N. Tereshchenko ◽  
...  
Keyword(s):  
Embryonic Mortality ◽  
Control Group ◽  
Intragastric Administration ◽  
Sterile Saline ◽  
Cadmium Compounds ◽  
Female Wistar Rats ◽  
Combined Action ◽  
Embryotoxic Effects ◽  
Experimental Group ◽  
Post Implantation

Given the increase in cadmium in the environment (air, soil, water) through modern industrial processes, the absorption of significant amounts from cigarette smoke is relevant to studying the effect of cadmium compounds on embryogenesis. The purpose of the study: experimental study and comparison of embryolethality and embryotoxicity of metal citrates depending on the duration of their intragastric administration (during 13 and 20 days of gestation). Materials and methods. The study was performed on 120 white adult female Wistar rats that weigh 170-200 g. They were divided into 6 groups due to the intragastric administration of solutions of the studied metals – rats treated with citrates: cadmium at a dose of 1.0 mg/kg – 1st group (nfemale = 20, nemb = 166); cadmium at a dose of 1.0 mg/kg and cerium (1.3 mg/kg) – 2nd group (nfemale = 20, nemb = 185); cadmium in a dose of 1.0 mg/kg and germanium (0.1 mg/kg) – 3rd group (nfemale = 20, nemb = 184); cadmium at a dose of 1.0 mg/kg and zinc (1.5 mg/kg) – 4th group (nfemale = 20, nemb = 179); cadmium at a dose of 1.0 mg/kg and nanocomposite (iodine + sulfur + selenium) at a dose of 2.0 mg/kg – 5th group (nfemale = 20, nemb = 180), 6th group – control (nfemale = 20, nemb = 212) – proportional volume of sterile saline in the same way. Females were divided into 2 subgroups of 10 animals each, depending on the duration of administration of test substances. The embryotoxic and embryolethal effects of the test substances were evaluated according to generally accepted criteria, which were calculated according to well-known formulas. Results and discussion. Cadmium compounds harm the embryogenesis of rats in the experimental groups and increase the rates of embryolethality. The most pronounced differences in these parameters concerning the control were found in animals with isolated exposure to cadmium citrate: the rates of total embryonic mortality increased by 4.0 times in both study periods of embryogenesis and 20% from the 13th to the 20th day; increased pre-implantation mortality by 6.0 times with an increase in post-implantation mortality by 3 times on the 13th day and by 15.0 times and 2.8 times on the 20th day of embryogenesis, respectively. Depending on the duration of introduction, the indicators of pre-implantation mortality increased by 25.0% with an increase in post-implantation mortality by 22.2% in the same period. The experimental group of exposure to cadmium citrate at a dose of 1.0 mg/kg recorded the lowest indicators of the number of live fetuses and intrauterine survival with the highest resorption rate studied of embryonic development. At the same time, in the groups of combined exposure to cadmium citrate with metal citrates, a decrease in the indicators of total embryonic mortality was 50.0% - 30.0%, pre-implantation mortality – 50.0% - 25.0%, post-implantation mortality – 60.0% - 44.4 % and increase in the number of fetuses per female – 12.7% - 25.3%. Conclusion. Analysis of the results shows a pronounced embryotoxic effect of cadmium citrate at a dose of 1.0 mg/kg on the processes of embryogenesis, which is a significant increase in overall embryonic mortality, preimplantation, and postimplantation mortality compared with the control group in all studied terms. In the groups of combined action of cadmium citrate with metal citrates, the data obtained indicate a decrease in the accumulation of cadmium under the influence of the studied citrates, which allows them to be considered as potential bioantagonists of cadmium citrate

scholarly journals The Effect of Thermal Conditioning on Thyroid Hormones, Hatchability and Embrionic Mortality of Broilers during the Incubation Period

2017 ◽  
Vol 66 (1-2) ◽  
pp. 32-37
Author(s):  
Zoran Ružić ◽  
Zdenko Kanački ◽  
Dragan Žikić ◽  
Gordana Ušćebrka
Keyword(s):  
Thyroid Gland ◽  
Embryonic Development ◽  
Incubation Period ◽  
Incubation Temperature ◽  
Embryonic Mortality ◽  
Control Group ◽  
Free T4 ◽  
Hormone Levels ◽  
Hatching Percentage ◽  
Positive Effect

SummaryThe aim of this study was to examine the effect of thermal conditioning on the embryonic mortality, on the time of embryonic mortality, as well as to examine the effect of this temperature regime on hatching and on thyroid gland hormone levels. 400 fertile eggs, randomly divided into two groups with two repetitions, were used in this research. The control group was exposed to the standard temperature (37.8°C) through the whole embryonic development. The second group was incubated under the standard conditions as the control group, up to 15thday of embryonic development. During 16th, 17thand 18thday of embryonic development, the incubation temperature was increased to 39°C for 3 hours. From 19thday up to the end of the incubation period the conditions of incubation were identical to those of the control group. Results of this study show that the applied treatment leads to an increase of concentration of free T3 hormone on the day 19 of embryonic development, as opposed to a decrease of free T4 hormone in the embryo’s blood compared to the control group. The percentage of hatchability was 82.2% in the control group and 83.3% in the second group. The result shows that this way of thermal manipulation did not affect the embryonic mortality, which was 9.5% in the control group and 8.9% in the experimental group. It can be concluded that in this manner thermal conditioning may manifest the positive effect on the thyroid gland hormone levels without increasing embryonic mortality or disturbing the hatching percentage.

scholarly journals EFFECT OF IONISED (ELECTROLYSED) WATER ON THE RAT EMBRYO DEVELOPMENT

2020 ◽  
Vol 28 (1) ◽  
pp. 28-35
Author(s):  
Jolita Stankevič ◽  
Aistė Audickaitė ◽  
Simona Šilovė ◽  
Valdas Šimčikas ◽  
Henrikas Cesiulis ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Tap Water ◽  
Wistar Rat ◽  
Female Rats ◽  
Control Group ◽  
Acidic Water ◽  
Domestic Water ◽  
Acidic Group ◽  
Halogenated Hydrocarbon ◽  
Negative Effect

The aim of this study was to investigate the effects ionised water has on embryonic development using Wistar rat animal model. For that purpose, alkaline and acidic water was prepared with a domestic water ioniser. It was found that the concentrations of Cl–, SO42– ions increased in acidic water, while in alkaline water, Ca2+ concentration decreased and halogenated hydrocarbon concentrations exceeded permitted levels. The animals were given test alkaline and acidic water, as well as tap water as control. After three months, female rats were mated. On the 21st day of gestation, they were euthanized and subjected to Caesarean sections; the number of live and dead fetuses was recorded. The fetuses were examined for external or visceral malformations and skeletal abnormalities. The data showed that embryo death was higher in acidic and alkaline experimental groups in comparison to the control group. The fetuses in both test groups were significantly shorter than in the control group. Long bones of fetal hind and front limbs were shorter in the acidic group in comparison to the control group. Retardation of limb osteogenesis was expressed in the acidic group fetuses. Therefore, in our model, ionised water had a negative effect on the embryonic development.

MODULATION OF TRIGLYCERIDES IN FEMALE RATTUS RATTUS WITH STEROID CONTRACEPTIVE ALGESTONE ACETOPHENIDE (DHPA)

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
ASHOK KUMAR ◽  
ALPANA PARMAR ◽  
ANAND KUMAR BAJPEYEE
Keyword(s):  
Mixed Type ◽  
Rattus Rattus ◽  
Young Female ◽  
Female Rats ◽  
Control Group ◽  
Black Rat ◽  
Long Acting ◽  
Steroid Contraceptive

Young female Black rat (Rattus rattus), were administered monthly long acting steroid contraceptive to induce hypertriglyceridemia. It was observed that by 3 weeks of the second injection of estrogen containing mixed type of contraceptive, female rats developed consistent and frank hyperglyceridemia . TG in the treated rats was 195.8 ± 7.44 mg /100 ml as compared to 91.5 ± 6.27 mg/100ml in plasma of the control group.

GENOTOXIC ACTIVITY OF THE PESTICIDE MIXTURES

2020 ◽  
pp. 9-13 ◽  
Author(s):  
N. A. Ilyushina ◽  
Yu. A. Revazova
Keyword(s):  
Public Health ◽  
Combined Effect ◽  
Literature Information ◽  
Genotoxic Effects ◽  
Antagonistic Effects ◽  
Pesticide Mixtures ◽  
The World ◽  
Living Organisms ◽  
Genetic Structures ◽  
Active Substances

In order to overcome resistance to individual pesticides and improve their effectiveness, formulations containing two or more active substances are constantly being developed and put on the market over recent years. Mixtures of residual amounts of pesticides can be present in water and food and enter the human and animal bodies. However, the combined effect of pesticides on living organisms, including genetic structures in cells, has not been studied enough and it is not yet possible to predict the genotoxic effects of their mixtures based on available data. The purpose of this review was to collect and summarize literature information on the genotoxicity of pesticide combinations obtained at different objects. The results of studies conducted in different countries of the world are discussed, examples of detected synergistic, additive and antagonistic effects are given, indicating the need for testing the genotoxicity of preparative forms of pesticides containing several active substances, as well as mixtures of jointly used pesticides in order to ensure the safe use of pesticides for public health.

scholarly journals Antioxidant activity of Lactobacillus plantarum NJAU-01 in an animal model of aging

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Qingfeng Ge ◽  
Bo Yang ◽  
Rui Liu ◽  
Donglei Jiang ◽  
Hai Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Lactobacillus Plantarum ◽  
Meat Product ◽  
The Other ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Intragastric Administration ◽  
Aging Effects ◽  
Sterile Saline

Abstract Background Excessive reactive oxygen species (ROS) can cause serious damage to the human body and may cause various chronic diseases. Studies have found that lactic acid bacteria (LAB) have antioxidant and anti-aging effects, and are important resources for the development of microbial antioxidants. This paper was to explore the potential role of an antioxidant strain, Lactobacillus plantarum NJAU-01 screened from traditional dry-cured meat product Jinhua Ham in regulating D-galactose-induced subacute senescence of mice. A total of 48 specific pathogen free Kun Ming mice (SPF KM mice) were randomly allocated into 6 groups: control group with sterile saline injection, aging group with subcutaneously injection of D-galactose, treatments groups with injection of D-galactose and intragastric administration of 107, 108, and 109 CFU/mL L. plantarum NJAU-01, and positive control group with injection of D-galactose and intragastric administration of 1 mg/mL Vitamin C. Results The results showed that the treatment group of L. plantarum NJAU-01 at 109 CFU/mL showed higher total antioxidant capacity (T-AOC) and the antioxidant enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) than those of the other groups in serum, heart and liver. In contrast, the content of the oxidative stress marker malondialdehyde (MDA) showed lower levels than the other groups (P < 0.05). The antioxidant capacity was improved with the supplement of the increasing concentration of L. plantarum NJAU-01. Conclusions Thus, this study demonstrates that L. plantarum NJAU-01 can alleviate oxidative stress by increasing the activities of enzymes involved in oxidation resistance and decreasing level of lipid oxidation in mice.

scholarly journals Effect of Honey and Syrup Diets Enriched with 1,3-1,6 β-Glucans on Honeybee Survival Rate and Phenoloxidase Activity (Apis mellifera L. 1758)

2021 ◽  
Vol 8 (7) ◽  
pp. 130
Author(s):  
Simona Sagona ◽  
Baldassare Fronte ◽  
Francesca Coppola ◽  
Elena Tafi ◽  
Matteo Giusti ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Survival Rate ◽  
Significant Variation ◽  
Innate Immune ◽  
Control Group ◽  
Phenoloxidase Activity ◽  
Immune Modulators ◽  
Negative Effect ◽  
Potential Negative Effect ◽  
Combined Action

β-glucans can activate the animal innate immune system by acting as immune-modulators and inducing various stimulatory effects. The aim of this study was to investigate the effect of 1,3-1,6 β-glucans administered orally for 96 h on Apis mellifera workers (newly emerged and nurse bees). β-glucans were included in honey and syrup. Survival rate and phenoloxidase activity were measured. In both newly emerged and nurse bees, β-glucans supplementation did not affect survival rate (p > 0.05). Conversely, phenoloxidase activity was higher in both newly emerged bees (p = 0.048) and nurse bees (p = 0.014) fed with a honey diet enriched with β-glucans compared to those fed with only honey. In both the newly emerged and nurse bees, no statistical differences in phenoloxidase activity were recorded between the group fed with a syrup-based diet enriched with β-glucans and the control group (p > 0.05). The absence of significant variation in survival suggests that the potential negative effect of β-glucans in healthy bees could be mitigated by their metabolism. Conversely, the inclusion of β-glucans in a honey-based diet determined an increase of phenoloxidase activity, suggesting that the effect of β-glucan inclusion in the diet of healthy bees on phenoloxidase activity could be linked to the type of base-diet. Further investigations on β-glucans metabolism in bees, on molecular mechanism of phenoloxidase activation by 1,3-1,6 β-glucans, and relative thresholds are desirable. Moreover, investigation on the combined action of honey and β-glucans on phenoloxidase activity are needed.

scholarly journals Folic Acid Affects Iron Status in Female Rats with Deficiency of These Micronutrients

2019 ◽  
Vol 195 (2) ◽  
pp. 551-558 ◽  
Author(s):  
Joanna Suliburska ◽  
Katarzyna Skrypnik ◽  
Agata Chmurzyńska
Keyword(s):  
Folic Acid ◽  
Iron Status ◽  
Iron Concentration ◽  
Folic Acid Supplementation ◽  
Female Rats ◽  
Control Group ◽  
Standard Diet ◽  
Study Group ◽  
Female Wistar Rats ◽  
Iron And Folic Acid

Abstract Although simultaneous supplementation with iron and folic acid is justified, the potential interactions between these micronutrients are unknown. The aim of this study was to determine the effects of oral iron and folic acid, administered together or separately, on iron concentration in tissues in rats with a deficiency of both these micronutrients. In the first stage of the experiment (28 days), 150 8-week-old female Wistar rats were randomly assigned to a control group (C; n = 30) fed the standard diet and to a study group (n = 120) fed a diet deficit in iron and folate. The study group was then randomly divided to four groups: D group fed a deficit diet, FE group fed a deficit diet with iron gluconate, the FOL group fed a deficit diet with folate acid, and the FEFOL group fed a deficit diet with iron gluconate and folate acid. After 2, 10, and 21 days of supplementation, ten animals from each group were killed. Morphological parameters were measured in whole blood. Iron concentration was assayed in serum, liver, spleen, pancreas, heart, and kidneys. Folic acid supplementation more significantly decreased iron concentrations in the pancreas and spleen than in the D group after 10 and 21 days of supplementation. Moreover, the combination of iron with folic acid markedly decreased iron levels in the liver and spleen, in comparison with iron alone, after 10 and 21 days of the experiment. In conclusion, folic acid affects iron status in female rats deficient in these micronutrients in moderate and long-term supplementation.

Effects of complex strength training with elastic band program on repeated change of direction in young female handball players: Randomized control trial

2021 ◽  
pp. 174795412110621
Author(s):  
Mehrez Hammami ◽  
Nawel Gaamouri ◽  
Yosser Cherni ◽  
Sabri Gaied ◽  
Mohamed Souhaiel Chelly ◽  
...  
Keyword(s):  
Strength Training ◽  
Young Female ◽  
Control Group ◽  
Countermovement Jump ◽  
Elastic Band ◽  
Squat Jump ◽  
Change Of Direction ◽  
Jump Test ◽  
Back Extensor ◽  
Experimental Group

This study examined the effect of 10-week complex strength training with elastic band program on fitness components in young female handball players. Thirty-eight handball players aged 15.8 ± 0.2 years were randomly assigned to an experimental group or control group. The experimental group performed complex strength training with elastic band twice a week over 10 weeks, which included 8 workshops of progressing set length (number of sets) and band resistance for each specific exercise. Sessions were lasted approximately 35 min. The control group maintained regular in-season training. Tests included handgrip; back extensor; medicine ball throw; 30 m sprint times; Modified Illinois change-of-direction (Illinois-MT); four jump tests (squat jump, countermovement jump, countermovement jump with arms and five jump test; static (stork test) and dynamic (Y balance test) balance; and repeated sprint T-test. The experimental group enhanced all strength performance (handgrip right ( p < 0.001), handgrip left ( p < 0.001), back extensor strength ( p < 0.001) and medicine ball throw ( p < 0.001) compared to the controls); sprint performance (5 m ( p<0.001), 10 m ( p < 0.001), 20 m ( p < 0.001), and 30 m ( p < 0.001)); the change of direction (Illinois-MT ( p < 0.001)); jump performance (squat jump ( p < 0.001), countermovement jump ( p < 0.001), countermovement jump with arms ( p < 0.001), and five jump test ( p < 0.01)); and the repeated sprint T-test scores ( p<0.001in all scores). In contrast, no significant difference in both static and dynamic balance performance between experimental group and control group. Ten weeks of complex strength training with elastic band improve fitness components measures in young female handball players then habitual training.

P–060 Dose- dependent mitigation of lead acetate toxicity in males on embryo development in female mice

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
P Dolati ◽  
M J Zamiri ◽  
A Akhlaghi ◽  
Z Jahromi
Keyword(s):  
Adverse Effects ◽  
Embryonic Development ◽  
Embryo Development ◽  
Lead Acetate ◽  
Endocrine System ◽  
Cell Number ◽  
The Other ◽  
Control Group ◽  
Male Mice ◽  
Female Mice

Abstract Study question Does quercetin (75 or 100 mg/kg BW/day) co-administration with lead acetate to male mice affects embryonic development in female mice? Summary answer The low-dose quercetin (75 mg/kg BW/day) ameliorated the adverse effects of lead acetate on mouse embryogenesis. What is known already Lead causes male infertility by impacting on endocrine system and spermatogenesis, and may exert undesirable effects on the offspring. The currently approved treatment for lead poisoning is the use of chelating agents, which form an insoluble complex with lead and shield it from biological targets; thus, reducing its toxicity. One of the main mechanisms of lead-induced toxicity is oxidative stress, and it has been reported that natural antioxidants can reduce the heavy metals toxicity. The aim of the present study was to examine the protective effects of quercetin on the toxicity induced by lead acetate on the embryogenesis in mice. Study design, size, duration Sexually mature (eight-week-old) NMRI male mice (n = 24) were randomly divided into four groups (n = 6 per group) receiving (i) distilled water (control group); (ii) lead acetate (150 mg/kg BW/day) dissolved in deionized water (LA); (iii) lead acetate (150 mg/kg BW/day) + quercetin (75 mg/kg BW/day) (LQ75); (IV) lead acetate (150 mg/kg BW/day) + quercetin (100 mg/kg BW/day) (LQ100). Treatments were applied daily as oral gavages for one cycle of the seminiferous epithelium (35 days). Participants/materials, setting, methods At the end of treatment administration, the males were joined with super-ovulated females, and the retrieved zygotes were cultured for evaluation of the embryo development (at 2-cell, 4-cell, 8-cell, and blastocyst stages), and blastocyst cell number using differential staining (propidium iodide and bisbenzimide). After incubation of capacitated sperm with oocytes, an ultraviolet light microscope was used following 3 min incubation with 25 µg⁄mL bisbenzamide solution for fertilization assessment. Main results and the role of chance Lead acetate (LA) treatment of male mice decreased the 2-cell stage compared with the control group (P &gt; 0.05). There was no difference between control and LQ75, and between LA and LQ100. The other stages of embryonic development were not significantly affected by the treatment. Overall, early embryonic development in the control and LQ75 mice were better than LQ100 and LA mice. The number of cells in the trophectoderm and inner-cell mass were not affected by treatments. However, the total blastocyst cell number in the control was higher than in the other groups; there was no significant difference between LQ100, LQ75 and LA groups. Fertilization rate was not affected by the treatments (P &lt; 0.05). Quercetin acts as a potent antioxidant at low doses, but at high doses exerts a pro-oxidant action. According to previous reports, higher concentrations of quercetin increased apoptosis and necrosis while decreasing the activities of the antioxidant enzymes. Also, it has been suggested that quercetin might disrupt the endocrine system and interfere with Sertoli cell function and sperm motility. Limitations, reasons for caution A limitation of this study is narrow dose selection; more studies are needed to determine the effective dose of quercetin in ameliorating the lead toxicity. There are also side effects of lead-quercetin chelates such as metal redistribution, essential metal loss, accumulation and persistency in intracellular sites, and peroxidation. Wider implications of the findings: Lead administration adversely impacted on the embryogenesis; on the other hand, paternal quercetin co-administration somewhat ameliorated the adverse effects of lead on mice embryogenesis. Trial registration number Not applicable

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